Single-Pollen Genotyping

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 4431539018
Total Pages : 134 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis Single-Pollen Genotyping by : Yuji Isagi

Download or read book Single-Pollen Genotyping written by Yuji Isagi and published by Springer Science & Business Media. This book was released on 2010-11-03 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pollination is one of the most important processes in plant reproduction. It directly influences reproductive success and fitness and the genetic structure of the plant population. Methods exist to infer the pattern and distance of pollen dispersal, but direct observation of the movements of individual pollen grains during pollination is not feasible owing to their small size. Single-pollen genotyping is a novel technique for genotyping a single pollen grain. In this book, the principles, the experimental protocol, and several applications of this method in studies of plant ecology, reproductive biology, and evolutionary genetics have been described. More specifically, the information is useful for the analysis of linkage disequilibrium, intraspecific genetic variation, chromosome mapping, and the origins of polyploidy. It is also essential for achieving sustainable and optimal crop yield and is vital to agriculture and forestry. Written by pioneer researchers, the book provides novel research approaches that are proving useful in a growing number of fields. This volume is intended to encourage new and continued applications of single-pollen genotyping among many disciplines in the future.

Development and Application of Crystal Digital PCR-based Single Pollen Nucleus Genotyping to Measure Meiotic Recombination Rates in Barley (Hordeum Vulgare) in High- Throughput

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (131 download)

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Book Synopsis Development and Application of Crystal Digital PCR-based Single Pollen Nucleus Genotyping to Measure Meiotic Recombination Rates in Barley (Hordeum Vulgare) in High- Throughput by : Yun-Jae Ahn

Download or read book Development and Application of Crystal Digital PCR-based Single Pollen Nucleus Genotyping to Measure Meiotic Recombination Rates in Barley (Hordeum Vulgare) in High- Throughput written by Yun-Jae Ahn and published by . This book was released on 2022* with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Barley, crossover, Crystal Digital PCRTM, Hordeum vulgare, meiosis, meiotic recombination, single pollen nucleus genotyping, single-cell analysis.

Assessment of Pre-PCR Whole Genome Amplification of Single Pollen Grains Using Flowering Dogwood (Cornus Florida)

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (51 download)

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Book Synopsis Assessment of Pre-PCR Whole Genome Amplification of Single Pollen Grains Using Flowering Dogwood (Cornus Florida) by : Candace Elizabeth Dillon

Download or read book Assessment of Pre-PCR Whole Genome Amplification of Single Pollen Grains Using Flowering Dogwood (Cornus Florida) written by Candace Elizabeth Dillon and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of gene flow in natural plant populations often focus on either historical or abiotic dispersal methods (e.g. wind, water, gravity), but there is little information available on contemporary, animal-mediated pollen dispersal patterns. Emerging molecular laboratory techniques allow unprecedented insights into spatial patterns of pollen-mediated gene flow. However, to date, technical challenges have limited their widespread application. The genome of a pollen grain can be amplified via whole genome amplification (WGA) prior to traditional amplification via polymerase chain reaction (PCR) to prevent the stochastic effects associated with low copy number amplification. Even still, WGA can suffer from low success rates or poor repeatability. The present study examined the extent to which WGA can be used to aid in understanding insect-mediated pollen flow in Cornus florida (flowering dogwood) within Virginia Commonwealth University's Inger and Walter Rice Center for Environmental Life Sciences. Initial amplification of DNA isolated from frozen grains was successful, until the pollen had been stored longer than 120 days at -20°C. After this time point, the PCR targets failed to amplify. The percent success of downstream PCR amplification on fresh pollen grains varied from 20% to 100%, with an average of 62% success. The addition of a common molecular crowder, polyethylene glycol, produced consistent amplification, regardless of input DNA concentration and eliminated the need for triplicate samples. Successful pollination and subsequent reproduction of flowering plants has a substantial ecological and agricultural importance that warrants increased understanding into how insects move pollen across the landscape. Determining the haploid profiles of a single pollen grain will allow scientists to elucidate dispersal patterns of pollen grains and track the movement and efficiency of biotic pollinators.

Safety of Genetically Engineered Foods

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Publisher : National Academies Press
ISBN 13 : 0309166152
Total Pages : 254 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Safety of Genetically Engineered Foods by : National Research Council

Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Plant Genotyping

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Author :
Publisher : CABI
ISBN 13 : 9780851998930
Total Pages : 352 pages
Book Rating : 4.9/5 (989 download)

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Book Synopsis Plant Genotyping by : Robert J. Henry

Download or read book Plant Genotyping written by Robert J. Henry and published by CABI. This book was released on 2001-08-17 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant genotyping, or DNA fingerprinting of plants, is a technology that has matured and is poised for widespread practical application in the fields of breeding, commerce and research. This book examines the technologies available and their application in the analysis of:Wild plant populationsGermplasm collections Plant breedingContributors include leading research workers in this field from North America, Europe and Australasia.

Genetics and Genomics of Plant Reproduction for Crop Breeding

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Author :
Publisher : Frontiers Media SA
ISBN 13 : 2889638871
Total Pages : 377 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis Genetics and Genomics of Plant Reproduction for Crop Breeding by : Gianni Barcaccia

Download or read book Genetics and Genomics of Plant Reproduction for Crop Breeding written by Gianni Barcaccia and published by Frontiers Media SA. This book was released on 2020-07-28 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Advances in Plant Meiosis: From Model Species to Crops

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Publisher : Frontiers Media SA
ISBN 13 : 2889634280
Total Pages : 267 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis Advances in Plant Meiosis: From Model Species to Crops by : Tomás Naranjo

Download or read book Advances in Plant Meiosis: From Model Species to Crops written by Tomás Naranjo and published by Frontiers Media SA. This book was released on 2020-01-28 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Integrative Observations and Assessments

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Publisher : Springer
ISBN 13 : 4431547835
Total Pages : 429 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis Integrative Observations and Assessments by : Shin-ichi Nakano

Download or read book Integrative Observations and Assessments written by Shin-ichi Nakano and published by Springer. This book was released on 2014-06-04 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on new trends in monitoring biodiversity in the Asia-Pacific region, one of the most rapidly changing areas in the world. It provides reviews of the challenges in studying the spatial variability of biodiversity across various ecosystems. This book also describes newly developed concepts and methods for biodiversity observation including ubiquitous genotyping, systematic conservation, monitoring of the functions and services of ecosystems and biodiversity informatics. These contributions will lead to establishing integrative observations and assessments of biodiversity, essential for reporting the current status and for the effective conservation and sustainable use of biodiversity. This work will interest biodiversity researchers not only in the Asia-Pacific region but also across the entire globe.

Paleogenomics

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Publisher : Springer
ISBN 13 : 3030047539
Total Pages : 427 pages
Book Rating : 4.0/5 (3 download)

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Book Synopsis Paleogenomics by : Charlotte Lindqvist

Download or read book Paleogenomics written by Charlotte Lindqvist and published by Springer. This book was released on 2019-01-07 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in genome-scale DNA sequencing technologies have revolutionized genetic research on ancient organisms, extinct species, and past environments. When it is recoverable after hundreds or thousands of years of unintended preservation, “ancient DNA” (or aDNA) is often highly degraded, necessitating specialized handling and analytical approaches. Paleogenomics defines the field of reconstructing and analyzing the genomes of historic or long-dead organisms, most often through comparison with modern representatives of the same or similar species. The opportunity to isolate and study paleogenomes has radically transformed many fields, spanning biology, anthropology, agriculture, and medicine. Examples include understanding evolutionary relationships of extinct species known only from fossils, the domestication of plants and animals, and the evolution and geographical spread of certain pathogens. This pioneering book presents a snapshot view of the history, current status, and future prospects of paleogenomics, taking a broad viewpoint that covers a range of topics and organisms to provide an up-to-date status of the applications, challenges, and promise of the field. This book is intended for a variety of readerships, including upper-level undergraduate and graduate students, professionals and experts in the field, as well as anyone excited by the extraordinary insights that paleogenomics offers.

Flow Cytometry with Plant Cells

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Publisher : John Wiley & Sons
ISBN 13 : 3527610936
Total Pages : 479 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Flow Cytometry with Plant Cells by : Jaroslav Dolezel

Download or read book Flow Cytometry with Plant Cells written by Jaroslav Dolezel and published by John Wiley & Sons. This book was released on 2007-06-27 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.

Plant Meiosis

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Author :
Publisher : Humana
ISBN 13 : 9781493998173
Total Pages : 410 pages
Book Rating : 4.9/5 (981 download)

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Book Synopsis Plant Meiosis by : Mónica Pradillo

Download or read book Plant Meiosis written by Mónica Pradillo and published by Humana. This book was released on 2019-10-23 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field.

Pollen and Spores

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Publisher : CRC Press
ISBN 13 : 1439843376
Total Pages : 412 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Pollen and Spores by : S N Agashe

Download or read book Pollen and Spores written by S N Agashe and published by CRC Press. This book was released on 2019-10-16 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Palynology finds applications in various fields. Some of them are taxonomy, plant evolution, plant breeding programmes, biotechnology, microbiology of water, soil and air, the pharmaceutical industry, cosmetic industry, energy food industry, forensic science, aerobiology, allergy, epidemiology, meteorology, fossil fuel exploration and biodiversity.

Plant Genotyping

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Author :
Publisher : Springer Nature
ISBN 13 : 1071630245
Total Pages : 464 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Plant Genotyping by : Yuri Shavrukov

Download or read book Plant Genotyping written by Yuri Shavrukov and published by Springer Nature. This book was released on 2023-02-13 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.

Proceedings of iMMM 2019 – International Molecular Mycorrhiza Meeting

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Publisher : Frontiers Media SA
ISBN 13 : 2889665011
Total Pages : 171 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis Proceedings of iMMM 2019 – International Molecular Mycorrhiza Meeting by : Paola Bonfante

Download or read book Proceedings of iMMM 2019 – International Molecular Mycorrhiza Meeting written by Paola Bonfante and published by Frontiers Media SA. This book was released on 2021-02-24 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Temperate Nuts

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Publisher : Springer Nature
ISBN 13 : 9811994978
Total Pages : 393 pages
Book Rating : 4.8/5 (119 download)

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Book Synopsis Temperate Nuts by : Mohammad Maqbool Mir

Download or read book Temperate Nuts written by Mohammad Maqbool Mir and published by Springer Nature. This book was released on 2023-05-02 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the production technology of temperate nuts. It explores cultivar and plant improvement, development and selection of rootstocks, plant–water relations and irrigation, canopy architecture, and postharvest packaging of nut fruits. It also deals with organic approaches, biotechnological interventions, diseases, and pest management. Contributing authors address nut fruits such as walnut, pecan nut, chestnut, and hazel nut on different recent aspects. The development achieved in the fruit sector is indicative of the fact that there is growing demand of fruit produce. Temperate nuts with health benefits and market acceptability have best sources of fat, fiber, protein, and mineral. They provide dietary fiber, potassium, and a variety of health-protective bioactive compounds. Apart from the health benefits, production of temperate nuts improves the economy of the country as these are very good source of income and employment. In addition, there is wide diversification in the production pattern of nuts globally. Increased production technology such as hybrids, high-yielding selections/ varieties, improved propagation techniques, and increased use of fertilizers and manures have brought spectacular increase in the production of nut fruits. This book is of interest to teachers, scientists, researchers, and scholars. Also, the book serves as additional reading material for undergraduate and postgraduate students of agriculture and horticulture. Horticulture nurseries and orchard industries also find this to be a useful read.

Genetics and Breeding for Productivity Traits in Forage and Bioenergy Grasses

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Author :
Publisher : MDPI
ISBN 13 : 3038425427
Total Pages : 213 pages
Book Rating : 4.0/5 (384 download)

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Book Synopsis Genetics and Breeding for Productivity Traits in Forage and Bioenergy Grasses by : John W. Forster

Download or read book Genetics and Breeding for Productivity Traits in Forage and Bioenergy Grasses written by John W. Forster and published by MDPI. This book was released on 2018-03-20 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Genetics and Breeding for Productivity Traits in Forage and Bioenergy Grasses" that was published in Agronomy

Advances in Plant Breeding Strategies: Fruits

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Publisher : Springer
ISBN 13 : 331991944X
Total Pages : 988 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis Advances in Plant Breeding Strategies: Fruits by : Jameel Al-Khayri

Download or read book Advances in Plant Breeding Strategies: Fruits written by Jameel Al-Khayri and published by Springer. This book was released on 2018-07-20 with total page 988 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the development of innovative modern methodologies towards augmenting conventional plant breeding for the production of new crop varieties, under the increasingly limiting environmental and cultivation factors, to achieve sustainable agricultural production and enhanced food security. Two volumes of Advances in Plant Breeding Strategies were published in 2015 and 2016, respectively; Volume 1: Breeding, Biotechnology and Molecular Tools and Volume 2: Agronomic, Abiotic and Biotic Stress Traits. This is Volume 3: Fruits, which is focused on advances in breeding strategies for the improvement of individual fruit crops. It consists of 23 chapters grouped into three parts, according to distribution classification of fruit trees: Part I, Temperate Fruits, Part II, Subtropical Fruits, and Part III, Tropical Fruits. Each chapter comprehensively reviews the modern literature on the subject and reflects the authors' own experience.