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Serological Detection Of Tomato Spotted Wilt Virus Tswv Proteins And Identification Of Components Of Tswv Acquisitions By Thrips
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Book Synopsis Serological Detection of Tomato Spotted Wilt Virus (TSWV) Proteins and Identification of Components of TSWV Acquisitions by Thrips by : Muralidhar Bandla
Download or read book Serological Detection of Tomato Spotted Wilt Virus (TSWV) Proteins and Identification of Components of TSWV Acquisitions by Thrips written by Muralidhar Bandla and published by . This book was released on 1997 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Tomato Spotted Wilt Virus Acquisition by Thrips by : Anna E. Whitfield
Download or read book Tomato Spotted Wilt Virus Acquisition by Thrips written by Anna E. Whitfield and published by . This book was released on 2004 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification, Cloning and Chracterization of a Putative Receptor for Tomato Spotted Wilt Virus in Its Primary Insect Vector by : Ricardo Brilhante de Medeiros
Download or read book Identification, Cloning and Chracterization of a Putative Receptor for Tomato Spotted Wilt Virus in Its Primary Insect Vector written by Ricardo Brilhante de Medeiros and published by . This book was released on 2000 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Epidemiological Analysis of Tomato Spotted Wilt Virus (TSWV) and Its Thrips Vectors in Crop Plants and Weed Hosts by : Ana Liza Carlobos Lopez
Download or read book Epidemiological Analysis of Tomato Spotted Wilt Virus (TSWV) and Its Thrips Vectors in Crop Plants and Weed Hosts written by Ana Liza Carlobos Lopez and published by . This book was released on 2007 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Tomato Spotted Wilt Virus by : Min Wang
Download or read book Tomato Spotted Wilt Virus written by Min Wang and published by . This book was released on 1990 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Induction and Suppression of Antiviral RNA Silencing by Tomato Spotted Wilt Virus by : Sergio M. Gabriel Peralta
Download or read book Induction and Suppression of Antiviral RNA Silencing by Tomato Spotted Wilt Virus written by Sergio M. Gabriel Peralta and published by . This book was released on 2017 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tomato spotted wilt virus (TSWV) is an emerging pathogen with wide host range and one of the most important viruses of plants. Information regarding processing of negative single stranded RNA viruses such as TSWV in the RNA silencing pathway remains limited. In nature TSWV is only transmitted by thrips as vectors and since infection occurs in both thrips and plants, an experimental system to transmit using thrips and the detection of TSWV were established. In order to understand the processing of TSWV in the RNA silencing pathway, Arabidopsis thaliana as a model plant was used in the genetic analysis against TSWV. Core components of the RNA silencing machinery tested were Dicer-like proteins(DCLs), RNA dependent RNA Polymerases (RDRs), and Argonaute proteins (AGOs). Results suggest DCL4 and DCL2 proteins are involved in the recognition and processing of TSWV. RDR1 is needed for the amplification of siRNAs derived from TSWV. AGOs protein levels remain unaffected after TSWV inoculation compared to mock inoculated plants suggesting that degradation by TSWV does not occur. It is well known that plant viruses encode suppressors of RNA silencing to facilitate plant infection and TSWV encodes the Non-Structural-small protein (NSs) whose mechanism of suppression has not been elucidated. NSs protein from TSWV was cloned from viral RNA and tagged with a 6HIS-3xFLAG tag at the C terminus for detection (NSs-HF). In transient assays activity of NSs as a suppressor of RNA silencing was corroborated by preventing silencing of green fluorescent protein (GFP) at three days post infiltration (dpi). Infectious clones of TSWV are not available and to determine the mechanism of suppression, NSs-HF was introduced in a previously described inactive model virus Turnip mosaic virus (TuMV-GFP-AS9) which has an inactive suppressor a does not infect wild type plants. The rescue of pathogenicity of this virus was observed after GFP detection under UV light and the presence of the NSs protein by western blotting. These experiments establish a foundation to study TSWV within the RNA silencing pathway and are the basis for future experiments to understand the mechanism of suppression of RNA silencing by TSWV.
Book Synopsis Genetic Determinants for Thrips Transmission of Tomato Spotted Wilt Virus by :
Download or read book Genetic Determinants for Thrips Transmission of Tomato Spotted Wilt Virus written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Tomato spotted wilt virus (TSWV) is the type species of the genus Tospovirus in the family Bunyaviridae and is exclusively transmitted by thrips in nature. To determine the viral RNA segment(s) responsible for thrips transmissibility, a viral genetic system that included reassortment and segment-specific RFLP analysis was developed. A complete set of reassortants between TSWV-RG2, a thrips transmissible isolate, and TSWV-D, a thrips nontransmissible isolate was generated. Thrips transmission assays of the reassortants showed that reassortants containing the M RNA from TSWV-D, a thrips nontransmissible isolate, were not transmissible, whereas reassortants with the M RNA from TSWV-RG2, a thrips transmissible isolate, were transmissible, irrespective of the origin of the L and S RNA. This is compelling evidence that thrips transmissibility of TSWV resides on the M RNA. Initial attempts to delineate specific regions of the M RNA associated with thrips transmission and characterize genetic changes on the M RNA of the TSWV following repeated mechanical or thrips transmission were made by comparing the nucleotide sequence of the M RNAs of the 3 poorly-transmissible TSWV isolates and transmissibility-restored isolates. After 24 transmission cycles by mechanical means or by thrips, whole M RNA sequences were determined by RT-PCR and sequencing. The mean mutation frequency of the NSm open reading frame (ORF) for the three isolates per passage was 9.2 X 10−5 nucleotide substitutions per base per mechanical or thrips passage. A similar pattern was observed in GN/GC ORF with 1.1 X 10−4 and 1.3 X 10−4 nucleotide substitutions per base per mechanical or thrips passage, respectively. Interestingly, two NS mutations and one synonymous S mutation in the G[subscript N]/G[subscript C] ORF were conserved in the three isolates after the series of mechanical transmission. Two NS mutations consist of a substitution of C86 to U (C86U) changing amino acid.
Book Synopsis Viruses and Virus Diseases of Vegetables in the Mediterranean Basin by : Gad Loebenstein
Download or read book Viruses and Virus Diseases of Vegetables in the Mediterranean Basin written by Gad Loebenstein and published by Academic Press. This book was released on 2012-06-25 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Advances in Virus Research focuses on mycoviruses. The authors and reviews represent the most current and cutting-edge research in the field. A broad range of research is presented from research experts. Contributions from leading authorities Informs and updates on all the latest developments in the field
Book Synopsis Changes in Thrips Pathogenicity and Transmissibility of Tomato Spotted Wilt Virus (TSWV) Following Repeated Mechanical Passage by : Brian Charles McNulty
Download or read book Changes in Thrips Pathogenicity and Transmissibility of Tomato Spotted Wilt Virus (TSWV) Following Repeated Mechanical Passage written by Brian Charles McNulty and published by . This book was released on 2001 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Distribution of Tomato Spotted Wilt Virus (TSWV) in Relation to Wild Weedy Hosts and Susceptible Crops Over a Large Agricultural Landscape by :
Download or read book Distribution of Tomato Spotted Wilt Virus (TSWV) in Relation to Wild Weedy Hosts and Susceptible Crops Over a Large Agricultural Landscape written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Tomato spotted wilt virus (TSWV) is an important agronomic and horticultural pest in NC and other parts of the world. Many have speculated that management of weedy species that host the virus may aid in decreasing TSWV occurrence on a farm. In May of 2003 and 2004, weedy species were sampled around six fields, two fields per replication, at the Center for Environmental Farming Systems (CEFS) in Goldsboro, NC, using a 15 m x 15 m grid to map the overwintering pattern of TSWV on the farm. Weeds were tested for TSWV infection and weed density ratings were assigned in each grid cell. Indicator plants (tomato and peanut) were used to map TSWV occurrence within the fields. Thrips movement was monitored with sticky traps to reveal spatial patterns of movement across the farm and, along with indictor plants, were evaluated biweekly through the summers of 2003 and 2004 to monitor temporal movement/occurrence. Plots were located at field edges and in field centers to detect the differences between trivial movement of infected thrips and movement up to 60 m from bordering weedy hosts. TSWV occurrence was equally likely to be found in the center versus the edge plots, revealing that complete eradication of host species up to 60 m from a field may not aid in decreasing TSWV in a field. TSWV occurrence in and around the three replications that were spread across the farm revealed an effect of replication in 2003 when more virus was found in the southern-most replication. Ranunculus sardous (hairy buttercup) tested positive most often compared to all other weed species tested and was also found in the highest density surrounding the southern-most replication.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1998 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Unveiling and Blocking the Interaction Between Tomato Spotted Wilt Virus and Its Insect Vector, Frankliniella Occidentalis by : Mauricio Montero Astúa
Download or read book Unveiling and Blocking the Interaction Between Tomato Spotted Wilt Virus and Its Insect Vector, Frankliniella Occidentalis written by Mauricio Montero Astúa and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Tomato spotted wilt virus (TSWV) is an economically important plant virus dependent on insects (thrips) for transmission to plant hosts. Like many animal-infecting viruses, TSWV replicates in the cells of its insect vector. The virus is an emergent disease threatening food and fiber crops worldwide. The aim of this work was to develop novel control strategies against TSWV through a better understanding of the virus-vector interaction. Previously, the TSWV GN protein was shown to be the viral attachment protein, a molecule mediating attachment of virus particles to the midgut epithelial cells of vector thrips. The specific goals of my research were to further examine the utility of disrupting the virus-vector interaction for effective virus control by exploiting GN properties, and to track the route of TSWV in thrips using confocal microscopy. To achieve these goals, I expressed soluble and insoluble forms of GN fused to green fluorescent protein (GFP) transiently and transgenically and examined their cellular localization in planta. GN::GFP recombinant protein localized to Golgi stacks throughout the cells as indicated by a punctate pattern or co-localization to a Golgi marker. In contrast, the soluble form of GN, GN-S::GFP, localized to the ER and apparently also to the cytoplasm. Virus acquisition and transmission assays with GN-S::GFP transgenic tomato plants demonstrated that transmission of TSWV by F. occidentalis was reduced by 35 to 100%. These results indicated that transgenic expression of GN-S in tomato plants may have the potential to prevent secondary spread of the virus. Novel features of the morphology of principal (PSGs) and tubular salivary glands (TSGs) of the insect vector F. occidentalis and of their infection with TSWV were described. The virus colonized different cell types and regions within the PSGs with variable intensity and distribution; and accumulated at the lumen of individual cells. The TSGs of F. occidentalis are proposed as a route for TSWV infection into the PSGs. The transgenic plants and the new knowledge of the virus vector interaction are promising tools to control TSWV and a model approach for the control of other vector-borne viruses.
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1997 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Principles of Molecular Virology by : Alan Cann
Download or read book Principles of Molecular Virology written by Alan Cann and published by Elsevier. This book was released on 2005-07-26 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Principles of Molecular Virology, Fourth Edition" provides an essential introduction to modern virology in a clear and concise manner. It is a highly enjoyable and readable text with numerous illustrations that enhance the reader's understanding of important principles. It contains new material on virus structure, virus evolution, zoonoses, bushmeat, SARS and bioterrorism. The standard version includes a CD-ROM with Flash animations, virtual interactive tutorials and experiments, self-assessment questions, useful online resources, along with the glossary, classification of subcellular infectious agents and history of virology.
Author :Richard Ellis Ford Matthews Publisher :Gulf Professional Publishing ISBN 13 :9780123611604 Total Pages :1084 pages Book Rating :4.6/5 (116 download)
Book Synopsis Matthews' Plant Virology by : Richard Ellis Ford Matthews
Download or read book Matthews' Plant Virology written by Richard Ellis Ford Matthews and published by Gulf Professional Publishing. This book was released on 2002 with total page 1084 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been ten years since the publication of the third edition of this seminal text on plant virology, during which there has been an explosion of conceptual and factual advances. The fourth edition updates and revises many details of the previous editon, while retaining the important older results that constitute the field's conceptual foundation. Key features of the fourth edition include: * Thumbnail sketches of each genera and family groups * Genome maps of all genera for which they are known * Genetic engineered resistance strategies for virus disease control * Latest understanding of virus interactions with plants, including gene silencing * Interactions between viruses and insect, fungal, and nematode vectors * New plate section containing over 50 full-color illustrations.
Download or read book The Potato Crop written by Hugo Campos and published by Springer Nature. This book was released on 2019-12-03 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY 4.0 license. This book provides a fresh, updated and science-based perspective on the current status and prospects of the diverse array of topics related to the potato, and was written by distinguished scientists with hands-on global experience in research aspects related to potato. The potato is the third most important global food crop in terms of consumption. Being the only vegetatively propagated species among the world’s main five staple crops creates both issues and opportunities for the potato: on the one hand, this constrains the speed of its geographic expansion and its options for international commercialization and distribution when compared with commodity crops such as maize, wheat or rice. On the other, it provides an effective insulation against speculation and unforeseen spikes in commodity prices, since the potato does not represent a good traded on global markets. These two factors highlight the underappreciated and underrated role of the potato as a dependable nutrition security crop, one that can mitigate turmoil in world food supply and demand and political instability in some developing countries. Increasingly, the global role of the potato has expanded from a profitable crop in developing countries to a crop providing income and nutrition security in developing ones. This book will appeal to academics and students of crop sciences, but also policy makers and other stakeholders involved in the potato and its contribution to humankind’s food security.
Book Synopsis Virus Diseases of Ornamental Plants by : S. K. Raj
Download or read book Virus Diseases of Ornamental Plants written by S. K. Raj and published by Springer Nature. This book was released on 2021-11-13 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book elucidates the evolution of plant virus, genomic structure, diversity, plant-virus interaction, subcellular movement etc. The book reviews the biological machineries which allow the emergence of virus populations adapted by plant. The main objective of this book is the demonstration of a clear synergistic effect of plant viruses, an effect that was unexpectedly as important as applied alone. Ornamental plants are very popular and economically important worldwide. The international market of ornamentals is constantly expanding. Viruses and viroids can significantly reduce both decorative value and quality of propagated material of ornamentals. Due to the wide range of ornamental plant species and cultivars and their wide geographical distribution, the diversity of viruses that infect them is also high. The new emerging viruses are the causal agent for the economic loss of many important ornamental plants. Therefore, this book also adds value to current knowledge of virus stress response in ornamental plants and will provide the groundwork necessary for building future strategies for product enhancement. This book is of interest to teachers, researchers, capacity builders and policymakers. It can serve as additional reading material for undergraduate and graduate students of virology, agriculture and plant sciences.
