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Author : Gary L. Juskowiak II
Publisher : Gary L. Juskowiak II
ISBN 13 :
Total Pages : 446 pages
Book Rating : 4./5 ( download)
Download or read book Sequence-Specific Reactivity of Short Peptides written by Gary L. Juskowiak II and published by Gary L. Juskowiak II. This book was released on 2009-05-27 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Gary Lee Juskowiak (II.)
Publisher :
ISBN 13 : 9781109154429
Total Pages : pages
Book Rating : 4.1/5 (544 download)
Download or read book Sequence-specific Reactivity of Short Peptides written by Gary Lee Juskowiak (II.) and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation covers the development of combinatorial peptide library methodology to screen for short peptides with enhanced and sequence-specific reactivity; the design, synthesis, and screening of small molecule fluorescent probes for short peptide tags; and the identification of peptides with enhanced and sequence-specific reactivity. Chapter 1 covers short peptides identified from a one-bead one-compound (OBOC) library that become fluorescent under photooxidative conditions. Only one peptide (AKPWGGDA) reproduced its fluorogenic reactivity in solution phase and the fluorescent photoproduct of this peptide was identified as a 4-aminoquinoline-2-carboxylic acid, which was presumably derived from tryptophan. Chapter 2 reviews the literature on peptide tags used for fluorescently labeling proteins in living cells with a specific emphasis on the biarsenical tetracysteine system. Chapter 3 reviews the literature on the sequence-specific structure and reactivity of short peptides. It also presents elements that may be applicable to enhanced and sequence-specific reactivity between short peptide tags and small molecule probes. Chapter 4 covers OBOC and SPOT peptide library methodology developed in order to screen for the sequence-specific reactivity of short peptides with small molecule fluorescent probes. These methodological advancements were primarily in the synthesis, screening, and tandem mass spectrometry sequencing of OBOC peptide libraries, and, in particular, cysteine-rich OBOC libraries. Chapter 5 covers the design, synthesis, and screening of small molecule fluorescent probes that contained the dansyl fluorophore and esters that target lysine residues in short peptide tags. Short peptides were discovered that exhibited enhanced and sequence-specific reactivity with these fluorescent probes, and, in particular, with a tetrahydroisoquinoline N-hydroxysuccinimide ester probe in an OBOC library, in a SPOT library, and in solution phase. However, the sequence-specific reactivity of the short peptide tags is reduced outside of the bead environment. Chapter 6 covers the design, synthesis, and screening of small molecule fluorogenic probes. These fluorogenic probes consisted of FlAsH, amino coumarin alkyl esters, and benzoyl quinoline carboxyaldehydes. Short peptides were discovered that exhibited enhanced and sequence-specific reactivity for FlAsH and the amino coumarin alkyl ester probes.
Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Gregg B. Fields
Publisher : Academic Press
ISBN 13 :
Total Pages : 828 pages
Book Rating : 4.X/5 (4 download)
Download or read book Solid-Phase Peptide Synthesis written by Gregg B. Fields and published by Academic Press. This book was released on 1997-10-21 with total page 828 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volumehas been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 275 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchers in all fields of life sciences. Key Features * Solid-phase peptide synthesis * Applications of peptides for structural and biological studies * Characterization of synthetic peptides
Author : Hans-Georg Rammensee
Publisher : Springer Science & Business Media
ISBN 13 : 3662221624
Total Pages : 462 pages
Book Rating : 4.6/5 (622 download)
Download or read book MHC Ligands and Peptide Motifs written by Hans-Georg Rammensee and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is centered on a comprehensive list of MHC peptide motifs and ligands as known to date, together with selected T cell epitopes, arranged in an easy-to-read fashion. This information is put into context by chapters on MHC gene organization, MHC structure, T cell epitope prediction, antigen processing and T cell responses. In addition, the book provides a great deal of complementary information: amino acid sequences of MHC class I alpha1 and alpha2 domains and of class II alpha1 and beta1 domains, the established or predicted composition and specificity of MHC pockets, notes on MHC nomenclature including old assignments and reference to useful internet addresses. A handy reference manual that should be helpful for all those dealing with MHC-associated peptides.
Author : John D. Roberts
Publisher :
ISBN 13 : 9780805383218
Total Pages : 1596 pages
Book Rating : 4.3/5 (832 download)
Download or read book Basic Principles of Organic Chemistry written by John D. Roberts and published by . This book was released on 1977 with total page 1596 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction what is organic chemistry all about?; Structural organic chemistry the shapes of molecules functional groups; Organic nomenclature; Alkanes; Stereoisomerism of organic molecules; Bonding in organic molecules atomic-orbital models; More on nomenclature compounds other than hydrocarbons; Nucleophilic substitution and elimination reactions; Separation and purification identification of organic compounds by spectroscopic techniques; Alkenes and alkynes. Ionic and radical addition reactions; Alkenes and alkynes; Oxidation and reduction reactions; Acidity or alkynes.
Author : Luca D. D'Andrea
Publisher : John Wiley & Sons
ISBN 13 : 1119044103
Total Pages : 492 pages
Book Rating : 4.1/5 (19 download)
Download or read book Chemical Ligation written by Luca D. D'Andrea and published by John Wiley & Sons. This book was released on 2017-04-03 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting a wide array of information on chemical ligation – one of the more powerful tools for protein and peptide synthesis – this book helps readers understand key methodologies and applications that protein therapeutic synthesis, drug discovery, and molecular imaging. • Moves from fundamental to applied aspects, so that novice readers can follow the entire book and apply these reactions in the lab • Presents a wide array of information on chemical ligation reactions, otherwise scattered across the literature, into one source • Features comprehensive and multidisciplinary coverage that goes from basics to advanced topics • Helps researchers choose the right chemical ligation technique for their needs
Author : B.J. Chapman
Publisher : Elsevier
ISBN 13 : 9780080526133
Total Pages : 162 pages
Book Rating : 4.5/5 (261 download)
Download or read book Advances in DNA Sequence-specific Agents written by B.J. Chapman and published by Elsevier. This book was released on 2002-09-17 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series encompasses design, synthesis, application, and analytical methods (including clinical and in vitro) for the study of these critical interactions. As our understanding of the genome and proteome expands, general developments in the field of DNA sequence specific interaction are likely to play an increasingly important role. Accordingly, manuscripts have been solicited from experts covering a diverse range of fields, reflecting the cross-disciplinary and dynamic nature of the series. Volume 4 describes work on the modification of DNA by AT specific anticancer drugs, DNA alkylation events which involve metabolite generation, DNA sequence recognition by two selective binders, bulged DNA microenvironments as molecular targets, DNA sequence specific binding by short peptides and the analysis of DNA-protein interactions using DNase I footprinting methodology. Features include: • Expert contributors from the Biomedical world • Emerging areas of drug design and therapeutic applications • Nucleic acid-protein interactions • Color graphics of molecular modeling analyses • New and emerging methodologies
Author : Ved Srivastava
Publisher : Royal Society of Chemistry
ISBN 13 : 1788014332
Total Pages : 572 pages
Book Rating : 4.7/5 (88 download)
Download or read book Peptide Therapeutics written by Ved Srivastava and published by Royal Society of Chemistry. This book was released on 2019-08-28 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide therapy has become a key strategy in innovative drug development, however, one of the potential barriers for the development of novel peptide drugs in the clinic is their deficiencies in clearly defined chemistry, manufacturing and controls (CMC) strategy from clinical development to commercialization. CMC can often become a rate-limiting step due to lack of knowledge and lack of a formal policy or guidelines on CMC for peptide-based drugs. Regulators use a risk-based approach, reviewing applications on a case-by-case basis. Peptide Therapeutics: Strategy and Tactics for Chemistry, Manufacturing, and Controls covers efficient manufacturing of peptide drug substances, a review of the process for submitting applications to the regulatory authority for drug approval, a holistic approach for quality attributes and quality control from a regulatory perspective, emerging analytical tools for the characterisation of impurities, and the assessment of stability. This book is an essential reference work for students and researchers, in both academia and industry, with an interest in learning about CMC, and facilitating development and manufacture of peptide-based drugs.
Author : Paul Lloyd-Williams
Publisher : CRC Press
ISBN 13 : 9780849391422
Total Pages : 306 pages
Book Rating : 4.3/5 (914 download)
Download or read book Chemical Approaches to the Synthesis of Peptides and Proteins written by Paul Lloyd-Williams and published by CRC Press. This book was released on 1997-04-23 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications. This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.
Author : Gunnar Houen
Publisher : Humana
ISBN 13 : 9781493929986
Total Pages : 0 pages
Book Rating : 4.9/5 (299 download)
Download or read book Peptide Antibodies written by Gunnar Houen and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.
Author : Yawen Bai
Publisher : Springer Science & Business Media
ISBN 13 : 1597451894
Total Pages : 332 pages
Book Rating : 4.5/5 (974 download)
Download or read book Protein Folding Protocols written by Yawen Bai and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a broad range of techniques to study, predict, and analyze the protein folding process. Protein Folding Protocols also provides sample approaches toward the prediction of protein structure starting from the amino acid sequence, in the absence of overall homologous sequences.
Author : M. Zouhair Atassi
Publisher : Springer Science & Business Media
ISBN 13 : 1468460005
Total Pages : 298 pages
Book Rating : 4.4/5 (684 download)
Download or read book Immunobiology of Proteins and Peptides VI written by M. Zouhair Atassi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The articles in this volume represent papers delivered by invited speakers at the 6th International Symposium on the Immunobiology of Proteins and Peptides. In addition, a few of the abstracts submitted by participants were scheduled for minisymposia and some of the authors, whose presentations were judged by the Scientific Council to be of high quality, were invited to submit papers for publication in this volume. This symposium was established in 1976 for the purpose of bringing together, once every two or three years, active investigators in the forefront of contemporary immunology, to present their findings and discuss t heir significance in the light of current concepts and to identify important new directions of investigation. The founding of the symposium was stimulated by the achievement of major breakthroughs in the understanding of the immune recognition of proteins and peptides. We believed that these breakthroughs will lead to the creation of a new generation of peptide reagents which should have enormous potential in biological, therapeutic and basic applications. This anticipated explosion has in fact since occurred and many applications of these pep tides are now being realized.
Author : Egisto Boschetti
Publisher : Newnes
ISBN 13 : 0124045960
Total Pages : 365 pages
Book Rating : 4.1/5 (24 download)
Download or read book Low-Abundance Proteome Discovery written by Egisto Boschetti and published by Newnes. This book was released on 2013-03-26 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics. Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progress Includes detailed protocols that address real-life needs in laboratory practice Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceutics Compiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins
Author : Simon Renshaw
Publisher : Scion Publishing Ltd
ISBN 13 : 1907904417
Total Pages : 289 pages
Book Rating : 4.9/5 (79 download)
Download or read book Immunohistochemistry written by Simon Renshaw and published by Scion Publishing Ltd. This book was released on 2006-12-20 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. Introduction (S Renshaw) 2. Antibodies for immunochemistry (C Onley) 3. The selection of reporter labels (S Mardle) 4. Immunochemical staining techniques (S Renshaw) 5. Multiple immunochemical staining techniques (I Jones) 6. Confocal microscopy and immunohistochemistry (M Cuttle) 7. Ultrastructural immunochemistry (J Skepper) 8. Image capture, analysis and quantification (D Tannahill) 9. Quality assurance in immunohistochemistry (P Jackson) 10. Automated immunochemistry (E Schenck) List of suppliers; Index
Author : Günther Jung
Publisher : John Wiley & Sons
ISBN 13 : 3527614907
Total Pages : 571 pages
Book Rating : 4.5/5 (276 download)
Download or read book Combinatorial Peptide and Nonpeptide Libraries written by Günther Jung and published by John Wiley & Sons. This book was released on 2008-09-26 with total page 571 pages. Available in PDF, EPUB and Kindle. Book excerpt: With combinatorial chemistry millions of organic compounds can be produced simultaneously, quickly, and in most cases by automated procedures. These compound libraries are a cost-effective resource for the pharmaceutical industry in their search for biologically active lead structures. Furthermore simultaneous parallel synthesis of single peptides and peptide libraries solve the problem of the worldwide increasing demand for peptides. The synthetic methods described here in detail contribute to a forward-looking technology that has a high impact for industrial and academic research. Fast and efficient analytical techniques are essential for using the complicated product mixtures and detecting by-products. Various synthetic approaches and technologies, mass spectrometry, and screening assays are discussed extensively. This book is a must and an indispensible source of information for every researcher in this rapidly developing field, which spans organic synthesis, biochemistry, biotechnology, pharmaceutical, medicinal, and clinical chemistry.
Author : Marc H. V. Van Regenmortel
Publisher : CRC Press
ISBN 13 : 9780849388651
Total Pages : 430 pages
Book Rating : 4.3/5 (886 download)
Download or read book Sructure of Antigens written by Marc H. V. Van Regenmortel and published by CRC Press. This book was released on 1992-02-03 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structure of Antigens discusses a variety of topics dealing with the structural basis of antigenicity. Topics include the analytical methods used to elucidate the structure of antigens, the structure of antibodies, the principles underlying modern immunoassays and the measurement of antibody binding affinity, and physicochemical principles and methodological aspects. The book also considers major groups of antigens distinguished by their functional activity and biological role (e.g., drugs, autoantigens, snake toxins, allergens) or by their association with particular biological systems (e.g., antigens of microorganisms). Structure of Antigens will provide a current, useful, reference for researchers and graduate students in all fields of biological science who need an overview of antigenic specificity. VOLUME 1
