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Resolution Improvement In Optical Microscopy By Use Of Multi Beam Interferometric Illumination
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Book Synopsis Resolution Improvement in Optical Microscopy by Use of Multi-beam Interferometric Illumination by : Jekwan Ryu
Download or read book Resolution Improvement in Optical Microscopy by Use of Multi-beam Interferometric Illumination written by Jekwan Ryu and published by . This book was released on 2003 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using a conventional system with a 0.98 NA objective. Further, changing the NA of the actual objective from 0.2 to 0.1 causes negligible change in resolution, demonstrating that resolution is primarily determined by the illumination produced by the cone of beams. Another restriction of conventional systems is removed by the use of reflective elements. The illumination system, which determines resolution, can be constructed using only reflective elements. Ultraviolet or x-ray illumination can be used with fluorescing targets to obtain resolution beyond what is possible using visible light. The well-known problems of refractive optics at short wavelengths can be avoided.
Book Synopsis Resolution Improvement in Fluorescence and Phase Optical Microscopy by : Emeric Mudry
Download or read book Resolution Improvement in Fluorescence and Phase Optical Microscopy written by Emeric Mudry and published by . This book was released on 2012 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Various fields of experimental science are constantly requiring smaller resolution for optical microscopy. In this thesis are presented several works for improving resolution in fluorescence microscopy and in Tomographic Diffraction Microscopy (TDM), an emerging phase microscopy technique. In the first part it is shown that one can improve the axial resolution in depositing the sample on a mirror. In confocal fluorescence microscopy, this is done by shaping the illumination beam with a Spatial Light Modulator. In TDM this is done by adapting the reconstruction method. Then algorithms are proposed for reconstructing high-resolution images from structured illumination measurements with unknown illumination fields, both in fluorescence imaging (blind-SIM algorithm) and in TDM. This allows a dramatical simplification of the experimental set-ups in fluorescence structured illumination and the image reconstruction of high optical index samples in TDM.
Book Synopsis Superresolution Optical Microscopy by : Barry R. Masters
Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
Book Synopsis Coherent Light Microscopy by : Pietro Ferraro
Download or read book Coherent Light Microscopy written by Pietro Ferraro and published by Springer Science & Business Media. This book was released on 2011-02-09 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.
Book Synopsis Optical Interferometry, 2e by : P. Hariharan
Download or read book Optical Interferometry, 2e written by P. Hariharan and published by Academic Press. This book was released on 2003-09-22 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology, sensor and measurement industries depend on these advances in optical interferometry for accuracy and profitability.
Book Synopsis Super Resolution Optical Imaging and Microscopy by : Junle Qu
Download or read book Super Resolution Optical Imaging and Microscopy written by Junle Qu and published by John Wiley & Sons. This book was released on 2023-12-11 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.
Book Synopsis Ultra-high Resolution Fluorescence Microscopy and Its Application in Biology by : Lin Shao
Download or read book Ultra-high Resolution Fluorescence Microscopy and Its Application in Biology written by Lin Shao and published by . This book was released on 2005 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several new resolution enhancement schemes for fluorescence microscopy are experimentally implemented and tested using beads and biological samples. The first scheme, 3D structured illumination microscopy (SIM), makes use of the simple fact that fluorescence emission is the multiplication of the illumination and the sample's dye structure. If the illumination is structured such as a sinusoidal pattern, the recorded image would contain high-frequency components encoded in terms of low frequencies. In so doing, normally undetectable high-resolution information is given a chance to appear in the observable region, and is thus made effectively detectable. We are able to double the resolution both laterally and axially of the conventional microscope with 3D SIM. The second scheme, called I5S, also uses structured illumination, but with a Sagnac-like interferometer setup consisting of two opposing objective lenses used for illumination and fluorescence detection. Interference in both illumination, which gives rise to more complicated structured illumination pattern than 3D SIM, and detection can be achieved. The combined interferometric effect is seven-fold enhancement in axial resolution as well as doubled lateral resolution. Finally, we introduce a more complicated I5S scheme in which both emission wavefronts, instead of just one of them, are recorded by two CCD cameras simultaneously. The complementary phase relationship between the two wavefronts can be utilized in estimating and then correcting the phase error often difficult to avoid in I5S experiments.
Book Synopsis Multi-view Microscopy and Multi-beam Manipulation for High-resolution Optical Imaging by : Jan Huisken
Download or read book Multi-view Microscopy and Multi-beam Manipulation for High-resolution Optical Imaging written by Jan Huisken and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Super-Resolution Microscopy by : Udo J. Birk
Download or read book Super-Resolution Microscopy written by Udo J. Birk and published by John Wiley & Sons. This book was released on 2017-07-21 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Book Synopsis Optics at the Nanometer Scale by : M. Nieto-vesperinas
Download or read book Optics at the Nanometer Scale written by M. Nieto-vesperinas and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optics at the Nanometer Scale: Imaging and Storing with Photonic Near Fields deals with the fundamentals of and the latest developments and applications of near-field optical microscopy, giving basic accounts of how and under what circumstances superresolution beyond the half- wavelength Rayleigh limit is achieved. Interferometric and fluorescence techniques are also described, leading to molecular and even atomic resolution using light. The storage of optical information at this level of resolution is also addressed.
Book Synopsis Multi-dimensional Imaging by : Bahram Javidi
Download or read book Multi-dimensional Imaging written by Bahram Javidi and published by John Wiley & Sons. This book was released on 2014-03-26 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a broad overview of advanced multidimensional imaging systems with contributions from leading researchers in the field Multi-dimensional Imaging takes the reader from the introductory concepts through to the latest applications of these techniques. Split into 3 parts covering 3D image capture, processing, visualization and display, using 1) a Multi-View Approach and 2.) a Holographic Approach, followed by a 3rd part addressing other 3D systems approaches, applications and signal processing for advanced 3D imaging. This book describes recent developments, as well as the prospects and challenges in advances in imaging sciences and engineering such as 3D image sensing, 3D holographic imaging, imaging applications for bio-photonics and 3D image recognition. Advanced imaging systems incorporate knowledge from various fields. It is a complex technology that combines physics, optics, signal processing, and image capture techniques. Provides a broad overview of advanced multidimensional imaging systems with contributions from leading researchers in the field. Integrates the background, introductory material with new advances in 3D imaging and applications. Covers the most recent technologies such as high speed digital holography, compressive sensing, real-time 3D integral imaging, 3D TV, photon counting imaging. To be available as an enhanced ebook with added functionality of colour films showing the effects of advanced 3D applications such as 3D microscopy, 3D biomedical imaging and 3D for security and defense applications. Acts as a single source reference to the rapidly developing field of 3D imaging technology. Provides supplementary material on a companion website including video clips, examples, numerical simulations, and experimental results to show the theoretical concepts. With contributions from leading researchers from across these fields, Multi-dimensional Imaging is a comprehensive reference for the imaging technology research community.
Book Synopsis New Techniques of Optical Microscopy and Microspectroscopy by : Cherry
Download or read book New Techniques of Optical Microscopy and Microspectroscopy written by Cherry and published by CRC Press. This book was released on 1991 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.
Author :Tomasz S. Tkaczyk Publisher :SPIE-International Society for Optical Engineering ISBN 13 : Total Pages :162 pages Book Rating :4.F/5 ( download)
Book Synopsis Field Guide to Microscopy by : Tomasz S. Tkaczyk
Download or read book Field Guide to Microscopy written by Tomasz S. Tkaczyk and published by SPIE-International Society for Optical Engineering. This book was released on 2010 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers major system components, including light sources, illumination layouts, microscope optics, and image detection electronics. This guide also provides a comprehensive overview of microscopy techniques, including bright field and dark field imaging, contrast enhancement methods (such as phase and amplitude contrast), DIC, polarization, and fluorescence microscopy. In addition, it describes scanning techniques (such as confocal and multiphoton imaging points); new trends in super-resolution methods (such as 4Pi microscopy, STED, STORM, and structured illumination); and array microscopy, CARS, and SPIM.
Book Synopsis Super-Resolution Microscopy by : Udo J. Birk
Download or read book Super-Resolution Microscopy written by Udo J. Birk and published by John Wiley & Sons. This book was released on 2017-12-04 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Book Synopsis Increasing Resolution and Light Efficiency in Fluorescence Microscopy by : Kai Wicker
Download or read book Increasing Resolution and Light Efficiency in Fluorescence Microscopy written by Kai Wicker and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structured illumination microscopy (SIM) can be used to enhance the lateral resolution and the sectioning capability in microscopic imaging. As a wide-field technique it may have advantages over scanning approaches such as stimulated emission depletion (STED) microscopy regarding acquisition time and bleaching. Similar to the generation of moire patterns, the structured illumination transposes high spatial frequency information in the sample into low spatial frequency information, which can then be recorded by the microscope and computationally recovered in order to generate high resolution images. However, numerous experimental factors can lead to the generation of artefacts in the final image. Therefore, sophisticated algorithms are needed for the reconstruction of high quality images from raw data. This thesis discusses the experi¬mental challenges of structured illumination microscopy and newly developed methods of addressing these in the reconstruction process. The final images show good quality and a resolution improvement of about a factor of two. -- Confocal microscopy is another technique used for achieving optical sectioning of biological specimen. By using a pinhole in the detection pathway out-of-focus light is blocked, leading to the desired sectioning effect. Closing the pinhole further also allows an enhancement of the lateral resolution; however this comes at the cost of strongly reduced light efficiency, as less light passes through the pinhole. Adding an interferom¬eter with relative image inversion optics between its two arms to the descanned output of a confocal microscope makes it possible to surpass the lateral resolution limit (closed pinhole) of confocal microscopes for large pinholes, significantly increasing the light efficiency of such a microscope. This thesis presents the theoretical description of this method as well as experimental results confirming it.
Book Synopsis Fluorescence Microscopy and Fluorescent Probes by : J. Slavík
Download or read book Fluorescence Microscopy and Fluorescent Probes written by J. Slavík and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Book Synopsis Coherent Light Microscopy by : Pietro Ferraro
Download or read book Coherent Light Microscopy written by Pietro Ferraro and published by Springer. This book was released on 2011-07-20 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.
