Resin Microscopy and On-Section Immunocytochemistry

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Publisher : Springer Science & Business Media
ISBN 13 : 3642974813
Total Pages : 225 pages
Book Rating : 4.6/5 (429 download)

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Book Synopsis Resin Microscopy and On-Section Immunocytochemistry by : Geoffrey R. Newman

Download or read book Resin Microscopy and On-Section Immunocytochemistry written by Geoffrey R. Newman and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.

Resin Microscopy and On-Section Immunocytochemistry

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 3642569307
Total Pages : 291 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Resin Microscopy and On-Section Immunocytochemistry by : Geoffrey R. Newman

Download or read book Resin Microscopy and On-Section Immunocytochemistry written by Geoffrey R. Newman and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since antibodies tagged with markers have been developed, immunocytochemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.

Microscopy, Immunohistochemistry, and Antigen Retrieval Methods

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Publisher : Springer Science & Business Media
ISBN 13 : 0306475995
Total Pages : 368 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Microscopy, Immunohistochemistry, and Antigen Retrieval Methods by : M.A. Hayat

Download or read book Microscopy, Immunohistochemistry, and Antigen Retrieval Methods written by M.A. Hayat and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

Biological Specimen Preparation for Transmission Electron Microscopy

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Publisher : Princeton University Press
ISBN 13 : 1400865026
Total Pages : 349 pages
Book Rating : 4.4/5 (8 download)

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Book Synopsis Biological Specimen Preparation for Transmission Electron Microscopy by : Audrey M. Glauert

Download or read book Biological Specimen Preparation for Transmission Electron Microscopy written by Audrey M. Glauert and published by Princeton University Press. This book was released on 2014-07-14 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Diagnostic Electron Microscopy

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Publisher : John Wiley & Sons
ISBN 13 : 1119973996
Total Pages : 492 pages
Book Rating : 4.1/5 (199 download)

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Book Synopsis Diagnostic Electron Microscopy by : John Stirling

Download or read book Diagnostic Electron Microscopy written by John Stirling and published by John Wiley & Sons. This book was released on 2013-01-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.

Autoradiography and Immunocytochemistry

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Publisher : Elsevier
ISBN 13 : 1483293874
Total Pages : 238 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Autoradiography and Immunocytochemistry by : M.A. Williams

Download or read book Autoradiography and Immunocytochemistry written by M.A. Williams and published by Elsevier. This book was released on 2013-10-22 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.

Immunohistochemistry and Immunocytochemistry

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Publisher : John Wiley & Sons
ISBN 13 : 1118717740
Total Pages : 248 pages
Book Rating : 4.1/5 (187 download)

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Book Synopsis Immunohistochemistry and Immunocytochemistry by : Simon Renshaw

Download or read book Immunohistochemistry and Immunocytochemistry written by Simon Renshaw and published by John Wiley & Sons. This book was released on 2017-02-15 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.

Correlative light and volume electron microscopy: Methods and applications

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Publisher : Frontiers Media SA
ISBN 13 : 283251605X
Total Pages : 161 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Correlative light and volume electron microscopy: Methods and applications by : Christopher Guerin

Download or read book Correlative light and volume electron microscopy: Methods and applications written by Christopher Guerin and published by Frontiers Media SA. This book was released on 2023-03-09 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant Microtechniques and Protocols

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Publisher : Springer
ISBN 13 : 3319199447
Total Pages : 576 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Plant Microtechniques and Protocols by : Edward Chee Tak Yeung

Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by Springer. This book was released on 2015-09-19 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Electron Microscopy Methods and Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592592015
Total Pages : 292 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri

Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Electron Microscopic Immunocytochemistry

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Author :
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 292 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Electron Microscopic Immunocytochemistry by : Julia M. Polak

Download or read book Electron Microscopic Immunocytochemistry written by Julia M. Polak and published by Oxford University Press, USA. This book was released on 1992 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.

Biological Field Emission Scanning Electron Microscopy

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Publisher : John Wiley & Sons
ISBN 13 : 1118663241
Total Pages : 752 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Biological Field Emission Scanning Electron Microscopy by : Roland A. Fleck

Download or read book Biological Field Emission Scanning Electron Microscopy written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-02-06 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Bancroft's Theory and Practice of Histological Techniques

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Publisher : Elsevier Health Sciences
ISBN 13 : 0702042269
Total Pages : 655 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Bancroft's Theory and Practice of Histological Techniques by : Kim S Suvarna

Download or read book Bancroft's Theory and Practice of Histological Techniques written by Kim S Suvarna and published by Elsevier Health Sciences. This book was released on 2012-10-26 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.

Bancroft's Theory and Practice of Histological Techniques E-Book

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Author :
Publisher : Elsevier Health Sciences
ISBN 13 : 0702068861
Total Pages : 672 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Bancroft's Theory and Practice of Histological Techniques E-Book by : Kim S Suvarna

Download or read book Bancroft's Theory and Practice of Histological Techniques E-Book written by Kim S Suvarna and published by Elsevier Health Sciences. This book was released on 2018-02-27 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: For 40 years, Bancroft’s Theory and Practice of Histological Techniques has established itself as the standard reference for histotechnologists and laboratory scientists, as well as histopathologists. With coverage of the full range of histological techniques used in medical laboratories and pathology departments, it provides a strong foundation in all aspects of histological technology – from basic methods of section preparation and staining, to advanced diagnostic techniques such as immunocytochemistry and molecular testing. This revised and updated 8th Edition by Kim S. Suvarna, Christopher Layton, and John D. Bancroft is a one-stop reference for all those involved with histological preparations and applications, from student to highly advanced laboratory professional.

Electron Microscopy

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Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763701925
Total Pages : 702 pages
Book Rating : 4.7/5 (19 download)

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Book Synopsis Electron Microscopy by : John J. Bozzola

Download or read book Electron Microscopy written by John J. Bozzola and published by Jones & Bartlett Learning. This book was released on 1999 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

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Publisher : Springer Science & Business Media
ISBN 13 : 146120139X
Total Pages : 277 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences by : Julian E. Beesley

Download or read book Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences written by Julian E. Beesley and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.

Molecular Medical Microbiology

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Publisher : Academic Press
ISBN 13 : 0123977630
Total Pages : 2214 pages
Book Rating : 4.1/5 (239 download)

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Book Synopsis Molecular Medical Microbiology by : Yi-Wei Tang

Download or read book Molecular Medical Microbiology written by Yi-Wei Tang and published by Academic Press. This book was released on 2014-09-14 with total page 2214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The molecular age has brought about dramatic changes in medical microbiology, and great leaps in our understanding of the mechanisms of infectious disease. Molecular Medical Microbiology is the first book to synthesise the many new developments in both molecular and clinical research in a single comprehensive resource. This timely and authoritative three-volume work is an invaluable reference source of medical bacteriology. Comprising more than 100 chapters, organized into 17 major sections, the scope of this impressive work is wide-ranging. Written by experts in the field, chapters include cutting-edge information, and clinical overviews for each major bacterial group, in addition to the latest updates on vaccine development, molecular technology and diagnostic technology. Topics covered include bacterial structure, cell function, and genetics; mechanisms of pathogenesis and prevention; antibacterial agents; and infections ranging from gastrointestinal to urinary tract, centrtal nervous system, respiratory tract, and more. The first comprehensive and accessible reference on molecular medical microbiology Full color presentation througout In-depth discussion of individual pathogenic bacteria in a system-oriented approach Includes a clinical overview for each major bacterial group Presents the latest information on vaccine development, molecular technology, and diagnostic technology More than 100 chapters covering all major groups of bacteria Written by an international panel of authors who are experts in their respective disciplines