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Author : Ziming Weng
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (139 download)
Download or read book Replication and Recombination of the Red Clover Necrotic Mosaic Virus (PHD). written by Ziming Weng and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Toba Abo El-Sood Mohamed Osman
Publisher :
ISBN 13 :
Total Pages : 496 pages
Book Rating : 4.:/5 (94 download)
Download or read book Biological Functions of the RNA Components of Red Clover Necrotic Mosaic Virus written by Toba Abo El-Sood Mohamed Osman and published by . This book was released on 1989 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Veronica Roshani Basnayake
Publisher :
ISBN 13 :
Total Pages : 207 pages
Book Rating : 4.:/5 (66 download)
Download or read book Identification and Characterization of the Red Clover Necrotic Mosaic Virus Origin of Assembly written by Veronica Roshani Basnayake and published by . This book was released on 2005 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: icosahedral, RNA, plant virus, packaging scheme, origin of assembly, Red clover necrotic mosaic virus.
Author : Władysław Błaszczak
Publisher :
ISBN 13 :
Total Pages : 8 pages
Book Rating : 4.:/5 (83 download)
Download or read book Some Properties of Red Clover Necrotic Mosaic Virus written by Władysław Błaszczak and published by . This book was released on 1980 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Dustin Michael Lockney
Publisher :
ISBN 13 :
Total Pages : 219 pages
Book Rating : 4.:/5 (756 download)
Download or read book Red Clover Necrotic Mosaic Virus written by Dustin Michael Lockney and published by . This book was released on 2011 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Li Na Loo
Publisher :
ISBN 13 :
Total Pages : 103 pages
Book Rating : 4.:/5 (297 download)
Download or read book Development of Red Clover Necrotic Mosaic Virus as a Multifunctional Nanoparticle written by Li Na Loo and published by . This book was released on 2007 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: nanoparticle, development of Red clover necrotic mosaic virus, drug delivery tool.
Author :
Publisher :
ISBN 13 :
Total Pages : 458 pages
Book Rating : 4.:/5 (94 download)
Download or read book Gene Expression and in Planta Movement of Red Clover Necrotic Mosaic Virus written by and published by . This book was released on 1998 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Stanton Martin Lowell
Publisher :
ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.:/5 (871 download)
Download or read book Structural Characterization of Red Clover Necrotic Mosaic Virus by Small Angle Neutron Scattering and X-ray Crystallography written by Stanton Martin Lowell and published by . This book was released on 2012 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
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ISBN 13 :
Total Pages : 538 pages
Book Rating : 4.:/5 (94 download)
Download or read book The Movement Protein and Symptomatology of Red Clover Necrotic Mosaic Virus written by and published by . This book was released on 1993 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Zhongguo Xiong
Publisher :
ISBN 13 :
Total Pages : 168 pages
Book Rating : 4.:/5 (193 download)
Download or read book Nucleotide Sequence and Organization of the Red Clover Necrotic Mosaic Virus Genome written by Zhongguo Xiong and published by . This book was released on 1988 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Leila L. Paje-Manalo
Publisher :
ISBN 13 :
Total Pages : 134 pages
Book Rating : 4.:/5 (186 download)
Download or read book Independent Replication of Red Clover Necrotic Mosaic Virus RNA-1 in Electroporated Host and Nonhost Nicotiana Sp. Protoplasts written by Leila L. Paje-Manalo and published by . This book was released on 1988 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Andrew Adipongs Vaewhongs
Publisher :
ISBN 13 :
Total Pages : 150 pages
Book Rating : 4.:/5 (327 download)
Download or read book The Movement of Red Clover Necrotic Mosaic Virus in Nicotiana Benthamiana Plants Transformed with the Movement Protein written by Andrew Adipongs Vaewhongs and published by . This book was released on 1995 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of the Red Clover Necrotic Mosaic Virus Origin of Assembly written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Red clover necrotic mosaic virus (RCNMV) is a single-stranded positive-sense RNA plant virus of the Dianthovirus genus, family Tombusviridae. Each RCNMV virion contains 180 subunits of the 37 kDa capsid protein (CP) forming a non-enveloped, isometric particle of T=3 quasi symmetry, 30-35 nm in diameter. The RCNMV genome consists of two RNAs, RNA-1 and RNA-2. RNA-1 codes for three proteins: i) p88, the RNA-dependent RNA polymerase, ii) p27, the replicase related protein and iii) the CP. RNA-2 is monocistronic and codes for the movement protein (MP). Currently, the RNA complement within RCNMV virions has not been determined. Density gradient centrifugation data has suggested the probability of a single type of particle, whereas the viral RNA profile from virions suggests otherwise. My thesis research has determined the RNA content within RCNMV virions after exposure to either heat or UV-irradiation. Both treatments result in the formation of a stable RNA-1: RNA-2 heterodimer. This leads to the conclusion that RCNMV virions co-package RNA-1 and RNA-2. Upon either treatment, RNA-2 multimers are also observed, suggesting the presence of particles packaging RNA-2 exclusively. These observations suggest that the RCNMV virion population consists of two distinct types of particles having similar densities. Based on the above RNA complement findings, I proceeded to delineate the specific viral sequences that determine the RNA content of an RCNMV virion. During virion assembly, the RCNMV CP must be able to distinguish and package both RNA-1 and RNA-2 while excluding heterologous host RNAs present in the cell. Viral CP subunits recognize specific cognate genomic sequences and/or structures that are unique to each viral genome. These are designated as origin of assembly sequences or packaging signals. To elucidate the assembly mechanism of RCNMV, I searched for the presence of distinct packaging signals on each of the genomic RNAs. Various constructs of RNA-1 and RNA-2 were test.
Author :
Publisher :
ISBN 13 :
Total Pages : 380 pages
Book Rating : 4.:/5 (94 download)
Download or read book Aspects of the Biology and Molecular Biology of Red Clover Necrotic Mosaic Virus written by and published by . This book was released on 1992 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Red Clover Necrotic Mosaic Virus as a Multifunctional Nanoparticle written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Red clover necrotic mosaic virus (RCNMV) is a member of the genus Dianthovirus and the family Tombusviridae. RCNMV is a plant virus with 180 identical capsid proteins (CP) arranged to form a T = 3 icosahedral symmetry. The capsid, with an outer diameter of 36 nm and an inner diameter of 17 nm, is enclosed to package two ssRNA: RNA-1 (3.9 kb) and RNA-2 (1.5 kb). In nature, a 20 nucleotide hairpin structure within the RNA-2 sequence trans-activator region forms a kissing loop complex with 8 nucleotides of the RNA-1 to serve as the origin of assembly (OAS). This pseudo-knot structure selectively recruits CP to initiate the virus assembly. In one of the research directions, an oligonucleotide mimic of the OAS sequence was attached to Au, CoFe2O4, and CdSe nanoparticles ranging from 3-15 nm, to trigger virus-like assembly by RCNMV CP. The formation of virus-like particles encapsidated nanoparticles was comparable in size to the native RCNMV. Attempts to encapsidate nanoparticles with diameters> 17 nm did not result in well-formed particles. The versatility of RCNMV capsid to package smaller cargo was explored. RCNMV experiences reversible swelling transition in a pH- and divalent ion-dependent manner. This transition leads to the reversible opening of pores (~13 Å) that permit infusion of molecules into the interior cavity. Comparison of three dyes, Rhodamine (positive charge), Luminarosine (neutral) and Fluorescein (negative charge) shows that infusion is controlled by electrostatic interactions. This method was applied to package an anticancer drug, Doxorubicin, within the capsid. The capability of the RCNMV capsid to deliver cargo into cancer cells was explored. Peptides from various serotypes of adenovirus were conjugated to the capsid to determine the internalization efficiency. The modified RCNMV was prepared with packaged molecules in the interior cavity and targeting peptide on the exterior surface. Intracellular uptake was studied both in terms of RCNMV interna.
Author : Katherine Anne Turner
Publisher :
ISBN 13 :
Total Pages : 139 pages
Book Rating : 4.:/5 (59 download)
Download or read book Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells written by Katherine Anne Turner and published by . This book was released on 2002 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: plant, remodeling, protein fusions, GFP, ER, viral polymerase, confocal microscopy.
Download or read book Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The replication of positive strand RNA viruses requires association with host membranes and frequently results in membrane proliferation and rearrangement. The fact that widely divergent virus families share form and function of replication strategies is likely due to common evolutionary origin and suggests that elucidating the reproduction strategies of one virus provides insight to virus life cycles in general. Red clover necrotic mosaic virus (RCNMV) is a positive-strand RNA virus in the Dianthovirus genus, Tombusviridae family. RCNMV encodes N-terminally overlapping proteins of 27 kDa and 88 kDa (p27 and p88). p88 contains motifs characteristic of RNA-dependent RNA polymerases, and while the function of p27 is unclear, it is very likely to be part of the RCNMV replication complex. I have investigated the localization of the RCNMV replicase proteins in plants using green fluorescent protein (GFP) fusions observed in live cells using confocal microscopy. The GFP:p27 and GFP:p88 fusions were expressed in N. benthamiana epidermal cells and protoplasts. GFP:p27 consistently localized to the endoplasmic reticulum (ER) and caused membrane restructuring and proliferation. Fractionation of virus-inoculated leaves demonstrated the association of p27 with isolated ER membranes. Additionally, GFP:p27 co-localized with ER-targeted YFP. GFP:p88 also localized to the ER and co-localized with GFP:p27. In addition, GFP:p27 and GFP:p88 were associated with invaginations of the nuclear membrane. These grooves and channels of ER crossed the nucleus, giving the appearance of fluorescence within the nucleus, while the signal from GFP:p27/p88 was actually associated with ER. GFP:p88 co-localized with GFP:p27 in two expression systems. This and the fact that GFP:p27 localized to the cortical ER, the nuclear envelope, and cytoplasmic ER domains in the presence of viral genomes as well as in the absence of any viral proteins suggests that the two proteins co-localize to the ER during inf.
