Recent Advances In Immunoassays

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Recent Advances in Immunoassays

Author : Samar K. Kundu
Publisher : Createspace Independent Publishing Platform
ISBN 13 : 9781500552312
Total Pages : 130 pages
Book Rating : 4.5/5 (523 download)

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Book Synopsis Recent Advances in Immunoassays by : Samar K. Kundu

Download or read book Recent Advances in Immunoassays written by Samar K. Kundu and published by Createspace Independent Publishing Platform. This book was released on 2014-07-16 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassay is a biochemical method that measures the presence or concentration of an analyte referred as an antigen in a solution through the use of an antibody or immunoglobulin. Immunoassays are now very widely used in the clinical laboratory, either because no other type of assay system is feasible or because they are often the most effective and suitable of the possible analytical methods. The major trend has been away from liquid-phase assays involving radioisotopic labels, towards fast homogeneous or solid-phase assays capable of operation anywhere; and towards precise and reliable non-isotopic, automated or semi-automated laboratory assays, often with detection limits measured in pico- or attomoles. The use of monoclonal antibodies is now widespread, and the methodologies of labels and of solid-phase components are much more sophisticated. New assay formulations, novel homogeneous systems, immunosensors, free-analyte assays, ultra-sensitive detection systems are being developed. Recent trends has been to enhance the sensitivity of the assays that may also have applications in measurement of other tumor markers, providing earlier detection of cancer or of relapse, Alzheimer's disease and in measurement of certain cardiac markers. More importantly, it is believed that high-sensitivity assays are expected to enable about a 50-fold expansion in the number of protein markers that can be analyzed in the clinical lab. At present, due to the sensitivity limitations of existing immunoassays of about 10-15 molar, only about 2,000 proteins are routinely analyzed by clinical labs. However, about 100,000 proteins are believed to exist in human blood, most of which are not analyzed due to limitations of existing assays. New high-sensitivity tests could potentially drive a significant expansion of the immunoassay market by enabling an entirely new set of markers to be analyzed. The present book is intended to overview different immunoassays including the most recent development trend

Advances in Immunoassay Technology

Author : Norman Chiu
Publisher : BoD – Books on Demand
ISBN 13 : 9535104403
Total Pages : 194 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Advances in Immunoassay Technology by : Norman Chiu

Download or read book Advances in Immunoassay Technology written by Norman Chiu and published by BoD – Books on Demand. This book was released on 2012-03-23 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the basic in vitro study of a specific biomolecule to the diagnosis or prognosis of a specific disease, one of the most widely used technology is immunoassays. By using a specific antibody to recognize the biomolecule of interest, relatively high specificity can be achieved by immunoassays, such that complex biofluids (e.g. serum, urine, etc.) can be analyzed directly. In addition to the binding specificity, the other key features of immunoassays include relatively high sensitivity for the detection of antibody-antigen complexes, and a wide dynamic range for quantitation. Over the past decade, the development and applications of immunoassays have continued to grow exponentially. This book focuses on some of the latest technologies for the development of new immunoassays.

Recent Advances in Immunoassays

Author : Dr Samar K Kundu Ph D
Publisher : CreateSpace
ISBN 13 : 9781500744397
Total Pages : 134 pages
Book Rating : 4.7/5 (443 download)

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Book Synopsis Recent Advances in Immunoassays by : Dr Samar K Kundu Ph D

Download or read book Recent Advances in Immunoassays written by Dr Samar K Kundu Ph D and published by CreateSpace. This book was released on 2014-08-04 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassay is a biochemical method that measures the presence or concentration of an analyte referred as an antigen in a solution through the use of an antibody or immunoglobulin. Immunoassays are now very widely used in the clinical laboratory, either because no other type of assay system is feasible or because they are often the most effective and suitable of the possible analytical methods. The major trend has been away from liquid-phase assays involving radioisotopic labels, towards fast homogeneous or solid-phase assays capable of operation anywhere; and towards precise and reliable non-isotopic, automated or semi-automated laboratory assays, often with detection limits measured in pico- or attomoles. The use of monoclonal antibodies is now widespread, and the methodologies of labels and of solid-phase components are much more sophisticated. New assay formulations, novel homogeneous systems, immunosensors, free-analyte assays, ultra-sensitive detection systems are being developed. Recent trends has been to enhance the sensitivity of the assays that may also have applications in measurement of other tumor markers, providing earlier detection of cancer or of relapse, Alzheimer's disease and in measurement of certain cardiac markers. More importantly, it is believed that high-sensitivity assays are expected to enable about a 50-fold expansion in the number of protein markers that can be analyzed in the clinical lab. At present, due to the sensitivity limitations of existing immunoassays of about 10-15 molar, only about 2,000 proteins are routinely analyzed by clinical labs. However, about 100,000 proteins are believed to exist in human blood, most of which are not analyzed due to limitations of existing assays. New high-sensitivity tests could potentially drive a significant expansion of the immunoassay market by enabling an entirely new set of markers to be analyzed. The present book is intended to overview different immunoassays including the most recent development trend.

Immunoassays

Author : Richard O'Kennedy
Publisher : CRC Press
ISBN 13 : 1351797344
Total Pages : 362 pages
Book Rating : 4.3/5 (517 download)

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Book Synopsis Immunoassays by : Richard O'Kennedy

Download or read book Immunoassays written by Richard O'Kennedy and published by CRC Press. This book was released on 2017-06-26 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept behind this book is to provide a detailed and practical overview of the development and use of immunoassays in many different areas. Immunoassays are analytical tests that utilise antibodies to measure the amount, activity or identity of an analyte. This book is designed to provide a critical and helpful insight into the subject and to give the user practical information that may be of assistance in assay format selection, antibody generation/selection and choice of appropriate detection strategies. It is comprised of 13 chapters written by highly experienced researchers in the fields of antibody-based research, immunoassay development, assay validation, diagnostics and microfluidics. Beginning with a comprehensive survey of antibodies, immunoassay formats and signalling systems, the book elucidates key topics related to the development of an ideal antibody-based sensor, focuses on the important topic of surface modification, explores key parameters in the immobilisation of antibodies onto solid surfaces, discusses the move to ‘lab-on-a-chip’-based devices and investigates the key parameters necessary for their development. Three of the chapters are dedicated to the areas of clinical diagnostics, infectious disease monitoring and food security, where immunoassay-based applications have become highly valuable tools. The future of immunoassays, including next-generation immunoassays, electrochemical-immunoassays and ‘lab-on-a-chip’-based systems, is also discussed. The book also covers the use of optical detection systems (with a focus on surface plasmon resonance) in immunoassays, provides a compilation of important, routinely used immunoassay protocols and addresses problems that may be encountered during assay development.

Enzyme Immunoassays

Author : S.S. Deshpande
Publisher : Springer Science & Business Media
ISBN 13 : 1461311691
Total Pages : 473 pages
Book Rating : 4.4/5 (613 download)

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Book Synopsis Enzyme Immunoassays by : S.S. Deshpande

Download or read book Enzyme Immunoassays written by S.S. Deshpande and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Handbook of Immunoassay Technologies

Author : Sandeep K. Vashist
Publisher : Academic Press
ISBN 13 : 012811794X
Total Pages : 498 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Handbook of Immunoassay Technologies by : Sandeep K. Vashist

Download or read book Handbook of Immunoassay Technologies written by Sandeep K. Vashist and published by Academic Press. This book was released on 2018-01-02 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. - Provides a valuable increase of understanding of cellular and biomedical functions - Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings - Discusses all multifarious aspects of immunoassays - Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications - Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays

The Immunoassay Handbook

Author : David Wild
Publisher : Newnes
ISBN 13 : 0080970389
Total Pages : 1037 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis The Immunoassay Handbook by : David Wild

Download or read book The Immunoassay Handbook written by David Wild and published by Newnes. This book was released on 2013-01-21 with total page 1037 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com

Advances in Immunoassay Technology

Author : Norman H. L. Chiu
Publisher :
ISBN 13 : 9789535152576
Total Pages : 192 pages
Book Rating : 4.1/5 (525 download)

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Book Synopsis Advances in Immunoassay Technology by : Norman H. L. Chiu

Download or read book Advances in Immunoassay Technology written by Norman H. L. Chiu and published by . This book was released on 2012 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the basic in vitro study of a specific biomolecule to the diagnosis or prognosis of a specific disease, one of the most widely used technology is immunoassays. By using a specific antibody to recognize the biomolecule of interest, relatively high specificity can be achieved by immunoassays, such that complex biofluids (e.g. serum, urine, etc.) can be analyzed directly. In addition to the binding specificity, the other key features of immunoassays include relatively high sensitivity for the detection of antibody-antigen complexes, and a wide dynamic range for quantitation. Over the past decade, the development and applications of immunoassays have continued to grow exponentially. This book focuses on some of the latest technologies for the development of new immunoassays.

Biosensing for the 21st Century

Author : Fred Lisdat
Publisher : Springer
ISBN 13 : 3540752013
Total Pages : 515 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Biosensing for the 21st Century by : Fred Lisdat

Download or read book Biosensing for the 21st Century written by Fred Lisdat and published by Springer. This book was released on 2007-11-20 with total page 515 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts

Biosensors Based on Sandwich Assays

Author : Fan Xia
Publisher : Springer
ISBN 13 : 9811078351
Total Pages : 220 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Biosensors Based on Sandwich Assays by : Fan Xia

Download or read book Biosensors Based on Sandwich Assays written by Fan Xia and published by Springer. This book was released on 2018-01-09 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.

Evidence-Based Medical Monitoring

Author : Paul P. Glasziou
Publisher : John Wiley & Sons
ISBN 13 : 0470695617
Total Pages : 376 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Evidence-Based Medical Monitoring by : Paul P. Glasziou

Download or read book Evidence-Based Medical Monitoring written by Paul P. Glasziou and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monitoring is a major component of management of chronic diseases such as diabetes, cardiovascular disease, arthritis and depression. Yet poor monitoring means healthcare costs are rising. This book discusses how monitoring principles adopted in other spheres such as clinical pharmacology and evidence-based medicine can be applied to chronic disease in the global setting. With contributions from leading experts in evidence-based medicine, it is a ground-breaking text for all involved in delivery of better and more effective management of chronic illnesses.

Lateral Flow Immunoassay

Author : Raphael Wong
Publisher : Springer Science & Business Media
ISBN 13 : 1597452408
Total Pages : 224 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Lateral Flow Immunoassay by : Raphael Wong

Download or read book Lateral Flow Immunoassay written by Raphael Wong and published by Springer Science & Business Media. This book was released on 2008-12-16 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.

Nonisotopic Immunoassay

Author : That T. Ngo
Publisher : Springer Science & Business Media
ISBN 13 : 1468454668
Total Pages : 487 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Nonisotopic Immunoassay by : That T. Ngo

Download or read book Nonisotopic Immunoassay written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.

Advanced Techniques in Immunoassays

Author : Jim Wang
Publisher :
ISBN 13 : 9781632390318
Total Pages : 0 pages
Book Rating : 4.3/5 (93 download)

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Book Synopsis Advanced Techniques in Immunoassays by : Jim Wang

Download or read book Advanced Techniques in Immunoassays written by Jim Wang and published by . This book was released on 2015-01-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassays are introduced in this book in a very sophisticated and concise manner. From the elementary in vitro analysis of a particular biomolecule to the diagnosis or prognosis of a particular disease, one of the most broadly practiced technologies is immunoassays. With the help of a particular antibody to identify the biomolecule of interest, comparatively high specificity can be attained by immunoassays, so that complex biofluids (e.g. serum, urine, etc.) can be examined directly. Along with the binding specificity, the other essential characteristics of immunoassays are relatively high sensitivity for the detection of antibody-antigen complexes, and a broad dynamic spectrum for quantitation. This book targets some of the recent technologies for the advancement of new immunoassays which, over the past decade, have continued to grow aggressively in their advancement and applications.

New Developments in Immunoassays

Author : Hans Schönfeld
Publisher :
ISBN 13 :
Total Pages : 157 pages
Book Rating : 4.:/5 (488 download)

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Book Synopsis New Developments in Immunoassays by : Hans Schönfeld

Download or read book New Developments in Immunoassays written by Hans Schönfeld and published by . This book was released on 1979 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mycotoxins in Fruits and Vegetables

Author : R. Barkai-Golan
Publisher : Elsevier
ISBN 13 : 0080557856
Total Pages : 407 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Mycotoxins in Fruits and Vegetables by : R. Barkai-Golan

Download or read book Mycotoxins in Fruits and Vegetables written by R. Barkai-Golan and published by Elsevier. This book was released on 2011-09-02 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mycotoxins are toxins produced by aerobic, microscopic fungus under special conditions of moisture and temperature. They colonize in a variety of foods from harvest to the grocer. Mycotoxins have gained world wide interest in recent years with the revelation of the effect of these toxins on health. A current example is the presence of ochratoxin A, a human carcinogen and nephrotoxin, in wines. The increased concern about fruit safety has led to increased studies throughout the world and enhanced awareness for stringent regulations governing mycotoxin limits in food.Presented in three defined sections, this is the first book to provide comprehensive analysis of the main mycotoxins contaminating fruits and vegetables and their derived products. The first section provides a safety evaluation of mycotoxins in fruits and vegetables, details regarding factors affecting mycotoxin production and diffusion in the fruit tissue, and recent methods for detection of mycotoxigenic fungi and mycotoxins produced by the fungi. The second part takes a critical look at the main individual mycotoxins and the third section focuses on approaches for prevention and control. - The first book dedicated to mycotoxins in fruits and vegetables - Presents mycological, mycotoxicological and phytopathological aspects of fruits and vegetables - Includes an analysis of detection, prevention and control methods for mycotoxigenic fungi and the mycotoxins they produce - Provides a complete risk assessment and safety evaluation of mycotoxins in perishable produce

Immunoassay and Other Bioanalytical Techniques

Author : Jeanette M. van Emon
Publisher : CRC Press
ISBN 13 : 1420020692
Total Pages : 536 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Immunoassay and Other Bioanalytical Techniques by : Jeanette M. van Emon

Download or read book Immunoassay and Other Bioanalytical Techniques written by Jeanette M. van Emon and published by CRC Press. This book was released on 2016-04-19 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Taking an interdisciplinary approach that emphasizes the adaptability of immunochemical and related bioanalytical methods to a variety of matrices, Immunoassay and Other Bioanalytical Techniques describes the strength and the versatility of these methods in a wide range of environmental and biological measurement applications. With contribut