Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis

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ISBN 13 :
Total Pages : 430 pages
Book Rating : 4.:/5 (479 download)

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Book Synopsis Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis by : Woei G. Tan

Download or read book Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis written by Woei G. Tan and published by . This book was released on 2000 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Capillary Electrophoresis of Nucleic Acids

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Publisher : Springer Science & Business Media
ISBN 13 : 1592590551
Total Pages : 481 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Capillary Gel Electrophoresis

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Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry

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ISBN 13 :
Total Pages : 89 pages
Book Rating : 4.:/5 (684 download)

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Book Synopsis DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry by :

Download or read book DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry written by and published by . This book was released on 1998 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first demonstration of the laser in the 1960's, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization/ionization interface and individual mammalian cells were analyzed. First, DNA Sanger fragments were separated by capillary gel electrophoresis. A separation speed of 20 basepairs per minute was demonstrated with a mixed poly(ethylene oxide) (PEO) sieving solution. In addition, a new capillary wall treatment protocol was developed in which bare (or uncoated) capillaries can be used in DNA sequencing. Second, a temperature programming scheme was used to separate DNA Sanger fragments. Third, flow cytometry and mass spectrometry were coupled with a laser vaporization/ionization interface.

Capillary Electrophoresis

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Publisher : Springer Science & Business Media
ISBN 13 : 1588295397
Total Pages : 808 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Capillary Electrophoresis by : Philippe Schmitt-Kopplin

Download or read book Capillary Electrophoresis written by Philippe Schmitt-Kopplin and published by Springer Science & Business Media. This book was released on 2008-02-25 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.

Capillary Electrophoresis of Nucleic Acids

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Publisher : Humana Press
ISBN 13 : 9781617371868
Total Pages : 484 pages
Book Rating : 4.3/5 (718 download)

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Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Humana Press. This book was released on 2010-10-28 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Genomic Signal Processing and Statistics

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Publisher : Hindawi Publishing Corporation
ISBN 13 : 9775945070
Total Pages : 456 pages
Book Rating : 4.7/5 (759 download)

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Book Synopsis Genomic Signal Processing and Statistics by : Edward R. Dougherty

Download or read book Genomic Signal Processing and Statistics written by Edward R. Dougherty and published by Hindawi Publishing Corporation. This book was released on 2005 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in genomic studies have stimulated synergetic research and development in many cross-disciplinary areas. Processing the vast genomic data, especially the recent large-scale microarray gene expression data, to reveal the complex biological functionality, represents enormous challenges to signal processing and statistics. This perspective naturally leads to a new field, genomic signal processing (GSP), which studies the processing of genomic signals by integrating the theory of signal processing and statistics. Written by an international, interdisciplinary team of authors, this invaluable edited volume is accessible to students just entering this emergent field, and to researchers, both in academia and in industry, in the fields of molecular biology, engineering, statistics, and signal processing. The book provides tutorial-level overviews and addresses the specific needs of genomic signal processing students and researchers as a reference book. The book aims to address current genomic challenges by exploiting potential synergies between genomics, signal processing, and statistics, with special emphasis on signal processing and statistical tools for structural and functional understanding of genomic data. The first part of this book provides a brief history of genomic research and a background introduction from both biological and signal-processing/statistical perspectives, so that readers can easily follow the material presented in the rest of the book. In what follows, overviews of state-of-the-art techniques are provided. We start with a chapter on sequence analysis, and follow with chapters on feature selection, classification, and clustering of microarray data. We then discuss the modeling, analysis, and simulation of biological regulatory networks, especially gene regulatory networks based on Boolean and Bayesian approaches. Visualization and compression of gene data, and supercomputer implementation of genomic signal processing systems are also treated. Finally, we discuss systems biology and medical applications of genomic research as well as the future trends in genomic signal processing and statistics research.

Single Cell Analysis by Capillary Electrophoresis

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Publisher :
ISBN 13 :
Total Pages : 270 pages
Book Rating : 4.:/5 (479 download)

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Book Synopsis Single Cell Analysis by Capillary Electrophoresis by : Zheru Zhang

Download or read book Single Cell Analysis by Capillary Electrophoresis written by Zheru Zhang and published by . This book was released on 2000 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cumulated Index Medicus

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ISBN 13 :
Total Pages : 1828 pages
Book Rating : 4.3/5 (243 download)

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Book Synopsis Cumulated Index Medicus by :

Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1828 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Rapid Cycle Real Time PCR

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Publisher : Springer Science & Business Media
ISBN 13 : 9783540206293
Total Pages : 240 pages
Book Rating : 4.2/5 (62 download)

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Book Synopsis Rapid Cycle Real Time PCR by : Carl Wittwer

Download or read book Rapid Cycle Real Time PCR written by Carl Wittwer and published by Springer Science & Business Media. This book was released on 2004 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier! TOC: Methods 1 Onno Bakker, Academic Medical Centre Amsterdam, NL Housekeeping Genes: A Gold Standard? 2 Weisser/ Schnittger, Klinikum Grosshadern München, Germany The choice of house keeping genes in MRD-quantification of t(8;21) positive AML 3 Ronald H. Lekanne-Deprez, Dep of Anatomie & Embryologie, Amsterdam, The Netherlands Quantification of mRNA Using Linear Regression of Log- Linear PCR Data-Points as an Alternative for the Standard Curve Approach 4 Jochen Wilhelm, University Giessen Estimation of Genome Sizes by Quantitative Real-Time PCR Applications Regulation and Development 5 N. Neubauer, University of Copenhagen, Biokemisk Afd., Copenhagen, Denmark Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I 6 Jürgen Loeffler, Medizinische Klinik, Abt. II, Otfried-Müller-Str. 10, 72076 Tübingen, Germany Quantification of T-Cell Receptor Excision Circle DANN Using Fluorescence Resonance Energy Transfer and the LightCycler System 7 Jim Whelan, Plant Molecular Biology Group, University of Western Australia, Crawley, Australia Investigation of Mitochondrial Biogenesis in Plants using Quantitative Real-Time PCR 8 E. Veistinen, Turku University, Dept. Medical Microbiology, Kiinamyllynkatu 13, FIN 20520 Turku Quantification of Ikaros Family Isoforms by Real-Time PCR 9 P. Stordeur, Dep. Immunologie- Hematologie-Transfusion, Hopital Erasme, Brussels, Belgium Methods to quantify cytokine gene expression by Real-Time PCR Oncology 10 Dr. Bernard, Idahotech, Salt Lake City, USA quantitative profiling for breast cancer using DNA and RNA markers 11 Melanie Königshoff, University Giessen Quantification of HER-2/NEU Gene Copy Number in Breast Cancer Tissue 12 Remedios Castelló Cros, Dpto. Bioquímica. Centro de Investigación, Hospital la Fe, Av. Campanar, 21, 46009 Valencia, Spain Quantitative real-time reverse transcription-PCR assay for urokinase plasminogen activator, plasminogen activator inhibitor type 1, and tissue metalloproteinase inhibitor type 1 gene expressions in primary breast cancer 13 C. H. W. Klaasen, C. Wilhelmina Hospital, Dep. Of Med. Microbiology & Infectious Diseases Nijmegen, NL Relative Quantification of Human DNA in Feaces (stool) 14 Chung-Che (Jeff) Chang, Assistant Professor, Director, Hematopathology Fellowship and Molecular/Pharmacogenetics Lab., Dep of Pathology, Medical College of Wisconsin, 9200 W. Wisconsin Ave., Milwaukee, WI 53226 real- time quatification of tumor load (t(14;18))in follicular lymphoma patients 15 P. Bolufer, Laboratorio de Biologia Molecular, Universitario La Fe, Valencia, Spain Real time quantification of AML rearrangements (AML1/ETO and TEL/AML1 ) in the diagnosis and monitoring of acute leukemia Genetics 16 Francisco Barros, INGO, Santiago de Compostela Gene Dosage Determination by Real Time PCR 17 Elaine Lyon, ARUP Laboratories, Salt Lake City, USA deletions and duplications of the cytochrome p450 2D6 gene using a reference gene and competitor (Alison Millson) 18 Karin Berg, Pathology, John Hopkins Medical Inst, Baltimore, USA Analysis of Bone Marrow Engraftment Following in Utero Bone Marrow Transplantation in a Can

Index Medicus

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Publisher :
ISBN 13 :
Total Pages : 1666 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Index Medicus by :

Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1666 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics

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Publisher : Elsevier Health Sciences
ISBN 13 : 0323548482
Total Pages : 1888 pages
Book Rating : 4.3/5 (235 download)

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Book Synopsis Tietz Textbook of Clinical Chemistry and Molecular Diagnostics by : Nader Rifai

Download or read book Tietz Textbook of Clinical Chemistry and Molecular Diagnostics written by Nader Rifai and published by Elsevier Health Sciences. This book was released on 2017-01-16 with total page 1888 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th Edition provides the most current and authoritative guidance on selecting, performing, and evaluating the results of new and established laboratory tests. This classic clinical chemistry reference offers encyclopedic coverage detailing everything you need to know, including: analytical criteria for the medical usefulness of laboratory tests, variables that affect tests and results, laboratory medicine, applications of statistical methods, and most importantly clinical utility and interpretation of laboratory tests. It is THE definitive reference in clinical chemistry and molecular diagnostics, now fully searchable and with quarterly content updates, podcasts, clinical cases, animations, and extended content online through Expert Consult. Analytical criteria focus on the medical usefulness of laboratory procedures. Reference ranges show new approaches for establishing these ranges — and provide the latest information on this topic. Lab management and costs gives students and chemists the practical information they need to assess costs, allowing them to do their job more efficiently and effectively. Statistical methods coverage provides you with information critical to the practice of clinical chemistry. Internationally recognized chapter authors are considered among the best in their field. Two-color design highlights important features, illustrations, and content to help you find information easier and faster. NEW! Internationally recognized chapter authors are considered among the best in their field. NEW! Expert Consult features fully searchable text, quarterly content updates, clinical case studies, animations, podcasts, atlases, biochemical calculations, multiple-choice questions, links to Medline, an image collection, and audio interviews. You will now enjoy an online version making utility of this book even greater. UPDATED! Expanded Molecular Diagnostics section with 12 chapters that focus on emerging issues and techniques in the rapidly evolving and important field of molecular diagnostics and genetics ensures this text is on the cutting edge and of the most value. NEW! Comprehensive list of Reference Intervals for children and adults with graphic displays developed using contemporary instrumentation. NEW! Standard and international units of measure make this text appropriate for any user — anywhere in the world. NEW! 22 new chapters that focus on applications of mass spectrometry, hematology, transfusion medicine, microbiology, biobanking, biomarker utility in the pharmaceutical industry and more! NEW! Expert senior editors, Nader Rifai, Carl Wittwer and Rita Horvath, bring fresh perspectives and help ensure the most current information is presented. UPDATED! Thoroughly revised and peer-reviewed chapters provide you with the most current information possible.

Ultrasensitive One- and Two- Dimensional Capillary Electrophoresis for Single Cell Analysis

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ISBN 13 :
Total Pages : 434 pages
Book Rating : 4.:/5 (733 download)

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Book Synopsis Ultrasensitive One- and Two- Dimensional Capillary Electrophoresis for Single Cell Analysis by : Md Abul Fazal

Download or read book Ultrasensitive One- and Two- Dimensional Capillary Electrophoresis for Single Cell Analysis written by Md Abul Fazal and published by . This book was released on 2005 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Dissertation Abstracts International

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ISBN 13 :
Total Pages : 960 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Dissertation Abstracts International by :

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2007 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection

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Publisher :
ISBN 13 :
Total Pages : 298 pages
Book Rating : 4.:/5 (356 download)

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Book Synopsis Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection by : Sheri Joanne Lillard

Download or read book Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection written by Sheri Joanne Lillard and published by . This book was released on 1996 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Encyclopedia of Forensic Sciences

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Publisher : Academic Press
ISBN 13 : 0123821665
Total Pages : 2253 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Encyclopedia of Forensic Sciences by :

Download or read book Encyclopedia of Forensic Sciences written by and published by Academic Press. This book was released on 2012-12-28 with total page 2253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic science includes all aspects of investigating a crime, including: chemistry, biology and physics, and also incorporates countless other specialties. Today, the service offered under the guise of "forensic science’ includes specialties from virtually all aspects of modern science, medicine, engineering, mathematics and technology. The Encyclopedia of Forensic Sciences, Second Edition, Four Volume Set is a reference source that will inform both the crime scene worker and the laboratory worker of each other’s protocols, procedures and limitations. Written by leading scientists in each area, every article is peer reviewed to establish clarity, accuracy, and comprehensiveness. As reflected in the specialties of its Editorial Board, the contents covers the core theories, methods and techniques employed by forensic scientists – and applications of these that are used in forensic analysis. This 4-volume set represents a 30% growth in articles from the first edition, with a particular increase in coverage of DNA and digital forensics Includes an international collection of contributors The second edition features a new 21-member editorial board, half of which are internationally based Includes over 300 articles, approximately 10pp on average Each article features a) suggested readings which point readers to additional sources for more information, b) a list of related Web sites, c) a 5-10 word glossary and definition paragraph, and d) cross-references to related articles in the encyclopedia Available online via SciVerse ScienceDirect. Please visit www.info.sciencedirect.com for more information This new edition continues the reputation of the first edition, which was awarded an Honorable Mention in the prestigious Dartmouth Medal competition for 2001. This award honors the creation of reference works of outstanding quality and significance, and is sponsored by the RUSA Committee of the American Library Association

Investigations Into the Use of Capillary Electrophoresis for the Analysis of Nuclear Preparations and the Nuclear Localization of Fusion Proteins at the Single Cell Level

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ISBN 13 :
Total Pages : 328 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Investigations Into the Use of Capillary Electrophoresis for the Analysis of Nuclear Preparations and the Nuclear Localization of Fusion Proteins at the Single Cell Level by : Nilhan Gunasekera

Download or read book Investigations Into the Use of Capillary Electrophoresis for the Analysis of Nuclear Preparations and the Nuclear Localization of Fusion Proteins at the Single Cell Level written by Nilhan Gunasekera and published by . This book was released on 2003 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: