Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Radiation Proteomics
Download Radiation Proteomics full books in PDF, epub, and Kindle. Read online Radiation Proteomics ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Radiation Proteomics by : Dariusz Leszczynski
Download or read book Radiation Proteomics written by Dariusz Leszczynski and published by Springer Science & Business Media. This book was released on 2013-02-03 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of proteomics have been shown to be powerful tools in search of target proteins – proteins that respond in cells to an internal or an external stimulus. Proteomics is widely used in biomedical research. However, in radiation biology research, following exposures of living matter to low doses of either ionizing or non-ionizing radiation, proteomics approach is only very slowly gaining support. This book, by presenting the current status of the use of proteomics in radiation biology, will help to attract attention to the field of radiation proteomics.
Book Synopsis Synchrotron Radiation and Structural Proteomics by : Eugenia Pechkova
Download or read book Synchrotron Radiation and Structural Proteomics written by Eugenia Pechkova and published by CRC Press. This book was released on 2011-11-11 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of the current state of research in both synchrotron radiation and structural proteomics from different laboratories worldwide. The book presents recent research results in the most advanced methods of synchrotron radiation analysis, protein micro- and nano crystallography, X-ray scattering and X-ray optics, coherent
Book Synopsis Proteomics and Nanocrystallography by : Eugenia Pechkova
Download or read book Proteomics and Nanocrystallography written by Eugenia Pechkova and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book addresses the most recent developments in structural and functional proteomics underlying the recent contributions given in these areas by our laboratory to the instrumentations, the methods and the procedures as mutuated from the nanoscale sciences and technologies. These developments introduced in the last few years make now possible protein massive identification (mass spectrometry and biomolecular arrays down to nanoamounts) and protein structural characterization in solution and in crystals down to the atomic scale to an extent and to a degree so far unmatched. Emphasis is placed in the growth by nanobiofilm template of protein crystals of any type and size from millimeter to micron, leading in combination with microfocus synchrotron technology and atomic force microscopy to the definition of a new field called nanocrystallography. The few useful examples being shown, concerning yet structurally unsolved proteins, point this very promising approach nanotechnology-based in structural proteomics using highly focused X-rays. This has not to be confused with the important study of nanocrystals, both organic and inorganic, and novel diamond like nanocomposite materials and devices having 3D protein crystals as matrices to be equilibrated with nanoparticles/gold/silver to be utilized in the most diversified electronic applications here also summarized. vii Acknowledgments We are particularly grateful to Giuseppe Zanotti at the University of Padova for his fundamental collaboration during all the crystallographic studies.
Book Synopsis High Throughput Analysis of Proteome Perturbations Induced by Radiation, Radiomitigators and Chemotherapeutics by : Jonathan Erde
Download or read book High Throughput Analysis of Proteome Perturbations Induced by Radiation, Radiomitigators and Chemotherapeutics written by Jonathan Erde and published by . This book was released on 2012 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics has flourished as a result of major advances in computational power, data storage technologies, and network architecture, all of which enable the acquisition, handling, and processing of enormous data sets. As mass spectrometers continue to break resolution and sensitivity barriers, the limitations of qualitative and quantitative proteomic experiments will no longer result from instrumentation constraints, but rather from the quality and availability of robust sample preparation methods and post-processing algorithms. My dissertation focuses on the development of technologies in experimental proteomic workflows that precede and that follow mass spectrometric analysis - areas that are integral to the future of proteomics. Enhanced Filter Aided Sample Preparation (eFASP) incorporates Tween-20 for plastic passivation, and n-octylglucoside and deoxycholic acid for digestion enhancement. In E. coli, eFASP reduces sample losses associated with normal FASP by almost 300%, and significantly increases hydrophobic protein representation when compared to normal FASP and to a routine precipitation-based sample preparation. eFASP is validated in the analysis of the ultra-stable proteinaceous sheath of Methanospirillum hungatei. eFASP and label-free quantitative analysis revealed a 40.7 kDa polypeptide (Q2FRN9) encoded by Mhun_2271 as the predominant sheath component by 10-fold. eFASP is combined with sample fractionation methods and software tools for the quantitative analysis of bacterial and human samples. Hydrophilic interaction chromatography (HILIC) enables small-scale fractionation for less complex samples: Syntrophus aciditrophicus, Anaerobaculum hydrogeniformans, and melanoma-derived exosomes. S. aciditrophicus and A. hydrogeniformans are profiled for proteome shifts resulting changes in carbon source. Treatment of melanoma cells with B-Raf inhibitor, PLX-4720, results in the packaging of two tyrosine kinases, VEGFR and PDGFR-B, into secreted exosomes. Upregulation of these receptor tyrosine kinases has been found to be a result of the release of feedback inhibition following treatment with MAPK targeted therapeutics. Complex whole-cell proteome analysis is effected by high-resolution electrostatic-repulsion hydrophilic interaction chromatography (ERLIC) fractionation of eFASP-processed samples. eFASP-ERLIC facilitated the quantitative analysis of normal and ATM-deficient human lymphoblastoid cell lines (LCLs) exposed to ionizing radiation (IR) and to YEL2, a potent radiomitigator. Exposure to IR resulted in the durable upregulation of NRF2-oxidative stress response components in Normal LCLs, while thrombin signaling and pro-survival proteins were upregulated in ATM-deficient LCLs. YEL2 treatment upregulated DNA repair components and enriched pro-survival pathways, which implicate the involvement of multiple processes in YEL2-mediated radioresistance.
Book Synopsis Proteomics Analysis of Human Endothelial Cells After Short-term Exposure to Mobile Phone Radiation by : Reetta Nylund
Download or read book Proteomics Analysis of Human Endothelial Cells After Short-term Exposure to Mobile Phone Radiation written by Reetta Nylund and published by . This book was released on 2011 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Radiation and the Immune System: Current Knowledge and Future Perspectives by : Katalin Lumniczky
Download or read book Radiation and the Immune System: Current Knowledge and Future Perspectives written by Katalin Lumniczky and published by Frontiers Media SA. This book was released on 2018-05-03 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: For long, high dose ionizing radiation was considered as a net immune suppressing agent, as shown, among others, by the exquisite radiosensitivity of the lymphoid system to radiation-induced cell killing. However, recent advances in radiobiology and immunology have made this picture more complex. For example, the recognition that radiation-induced bystander effects, share common mediators with various immunological signalling processes, suggests that they are at least partly immune mediated. Another milestone was the finding, in the field of onco-immunology, that local tumor irradiation can modulate the immunogenicity of tumor cells and the anti-tumor immune responsiveness both locally, in the tumor microenvironment, and at systemic level. These observations paved the way for studies exploring optimal combinations of radiotherapy and immunotherapy in order to achieve a synergistic effect to eradicate tumors. However, not all interactions between radiation and the immune system are beneficial, as it was recognized that many of radiation-induced late side effects are also of immune and inflammatory nature. Currently perhaps the most studied field of research in radiation biology is focused around the biological effects of low doses, where many of the observed pathophysiological endpoints are due to mechanisms other than direct radiation-induced cell killing and are immune-related. Finally, it must not be forgotten that the interactions between the ionizing radiations and the immune system are bi-directional, and activation of the immune system also influences the outcome of radiation exposure. This Research Topic brings together 23 articles and aims to give an overview of the complex and very often contradictory nature of the interactions between ionizing radiation and the immune system. Due to its increasing penetrance in the population both through medical diagnostic or environmental sources or during cosmic travel low dose ionizing radiation exposure is becoming a major epidemiological concern world-wide. Several of the articles within the Research Topic specifically address potential long-term health consequences and the underlying mechanisms of low dose radiation exposure. A major intention of the Editors was also to draw the attention of the non-radiobiological scientific community on the fact that ionizing radiation is by far more than purely an immune suppressing agent.
Book Synopsis Nuclear Analytical Techniques for Metallomics and Metalloproteomics by : Chunying Chen
Download or read book Nuclear Analytical Techniques for Metallomics and Metalloproteomics written by Chunying Chen and published by Royal Society of Chemistry. This book was released on 2010 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear analytical techniques have many advantages over other techniques, such as high sensitivity and precision. They couple powerful selective separation with sensitive element-specific detection. The uses of metalloproteomics studies are restricted to the fields of analytical and nuclear chemistry. They also have great potential to elucidate the origins of certain diseases and assist in their diagnosis and treatment via the development of new drugs. Nuclear Analytical Techniques for Metallomics and Metalloproteomics provides readers with a comprehensive view of this relatively new and exciting area of bioanalytical and inorganic chemistry. It contains contributions from experts in disciplines as diverse as analytical chemistry, nuclear chemistry, environmental science, molecular biology and medicinal chemistry. Various nuclear analytical techniques are covered including neutron activation analysis, X-ray fluorescence, isotope tracer, M÷ssbauer spectrometry, X-ray absorption spectrometry, and neutron scattering and diffraction. They provide useful information both for chemical speciation analysis and structural characterization of metalloproteins and metals in biological systems. Consequently, the book is not only relevant for chemists involved in nuclear techniques and speciation, but also environmental, nutritional and clinical researchers and drug developers. The book includes many illustrations, tables and documents to support the coverage of the latest developments. It also offers a well-organized bibliography to facilitate further reading.
Author :Gustavo García Gómez-Tejedor Publisher :Springer Science & Business Media ISBN 13 :9400725639 Total Pages :508 pages Book Rating :4.4/5 (7 download)
Book Synopsis Radiation Damage in Biomolecular Systems by : Gustavo García Gómez-Tejedor
Download or read book Radiation Damage in Biomolecular Systems written by Gustavo García Gómez-Tejedor and published by Springer Science & Business Media. This book was released on 2012-01-05 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of X-rays and radioactivity, ionizing radiations have been widely applied in medicine both for diagnostic and therapeutic purposes. The risks associated with radiation exposure and handling led to the parallel development of the field of radiation protection. Pioneering experiments done by Sanche and co-workers in 2000 showed that low-energy secondary electrons, which are abundantly generated along radiation tracks, are primarily responsible for radiation damage through successive interactions with the molecular constituents of the medium. Apart from ionizing processes, which are usually related to radiation damage, below the ionization level low-energy electrons can induce molecular fragmentation via dissociative processes such as internal excitation and electron attachment. This prompted collaborative projects between different research groups from European countries together with other specialists from Canada, the USA and Australia. This book summarizes the advances achieved by these research groups after more than ten years of studies on radiation damage in biomolecular systems. An extensive Part I deals with recent experimental and theoretical findings on radiation induced damage at the molecular level. It includes many contributions on electron and positron collisions with biologically relevant molecules. X-ray and ion interactions are also covered. Part II addresses different approaches to radiation damage modelling. In Part III biomedical aspects of radiation effects are treated on different scales. After the physics-oriented focus of the previous parts, there is a gradual transition to biology and medicine with the increasing size of the object studied. Finally, Part IV is dedicated to current trends and novel techniques in radiation reserach and the applications hence arising. It includes new developments in radiotherapy and related cancer therapies, as well as technical optimizations of accelerators and totally new equipment designs, giving a glimpse of the near future of radiation-based medical treatments.
Book Synopsis Advances in Radiation Biology by : John T. Lett
Download or read book Advances in Radiation Biology written by John T. Lett and published by Academic Press. This book was released on 2013-10-22 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Radiation Biology, Volume 9, provides an overview of the state of knowledge in radiation biology. The book contains nine chapters and begins with a study on the ways in which physical and chemical agents might trigger "regulatory dysfunction" and how these agents might interact with each other. This is followed by separate chapters on the mechanisms underlying changes in vascular function after doses of radiation in the therapeutic range and their role in the development of late effects in normal tissues; the future of hypoxic cell sensitizers in the clinical setting; DNA strand break formation by ionizing radiation; and major pathways which result in radiation-induced loss of cellular proliferative capacity. Subsequent chapters deal with the solid-state radiation chemistry of DNA; radiosensitivity of proliferating mammalian cells; the use of microwave/radiofrequency energy cancer treatment; and the decline of basic radiobiology.
Book Synopsis Molecular Radiation Biology by : Hermann Dertinger
Download or read book Molecular Radiation Biology written by Hermann Dertinger and published by Springer Science & Business Media. This book was released on 2013-11-09 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: There can hardly be any doubt that radiation will continue to be an im portant factor in our lives. Present and future advances in atomic tech nology urgently require further work on research and development in the field of radiation biology if the maximum benefit is to be obtained at minimal risk from the various kinds of radiation that form a major by product of nuclear processes. Consequently, it is also necessary to prepare students and younger scientists for doing such work. The present book originates from teaching experience gained in lectures, seminars, and discussion groups started by the undersigned in 1957 and more recently held together with Drs. Dertinger and Jung. The friendly comments given to the German edition made us feel that it might be worth while to put the results of our efforts at the disposal of those to whom English is more familiar. In agreement with the view, based on well-known facts, that most if not aIl of the more striking practical achievements have resulted from patient and careful investigations into some basic problem, the book aims at introducing the reader to the methods of thought and experiment used in molecular radiation biology as weIl as to the results obtained thereby.
Book Synopsis Genomics and Proteomics Engineering in Medicine and Biology by : Metin Akay
Download or read book Genomics and Proteomics Engineering in Medicine and Biology written by Metin Akay and published by John Wiley & Sons. This book was released on 2006-10-09 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current applications and recent advances in genomics and proteomics Genomics and Proteomics Engineering in Medicine and Biology presents a well-rounded, interdisciplinary discussion of a topic that is at the cutting edge of both molecular biology and bioengineering. Compiling contributions by established experts, this book highlights up-to-date applications of biomedical informatics, as well as advancements in genomics-proteomics areas. Structures and algorithms are used to analyze genomic data and develop computational solutions for pathological understanding. Topics discussed include: Qualitative knowledge models Interpreting micro-array data Gene regulation bioinformatics Methods to analyze micro-array Cancer behavior and radiation therapy Error-control codes and the genome Complex life science multi-database queries Computational protein analysis Tumor and tumor suppressor proteins interactions
Book Synopsis Microwave Assisted Proteomics by : Jennie R. Lill
Download or read book Microwave Assisted Proteomics written by Jennie R. Lill and published by Royal Society of Chemistry. This book was released on 2009-05-15 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an introduction to microwave-assisted bio-analytical methods with an emphasis on sample preparation for proteomic analyses. It includes a practical protocol section, allowing researchers to carry out the experiments in their own laboratory.
Book Synopsis Advancing Nuclear Medicine Through Innovation by : National Research Council
Download or read book Advancing Nuclear Medicine Through Innovation written by National Research Council and published by National Academies Press. This book was released on 2007-09-11 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nearly 20 million nuclear medicine procedures are carried out each year in the United States alone to diagnose and treat cancers, cardiovascular disease, and certain neurological disorders. Many of the advancements in nuclear medicine have been the result of research investments made during the past 50 years where these procedures are now a routine part of clinical care. Although nuclear medicine plays an important role in biomedical research and disease management, its promise is only beginning to be realized. Advancing Nuclear Medicine Through Innovation highlights the exciting emerging opportunities in nuclear medicine, which include assessing the efficacy of new drugs in development, individualizing treatment to the patient, and understanding the biology of human diseases. Health care and pharmaceutical professionals will be most interested in this book's examination of the challenges the field faces and its recommendations for ways to reduce these impediments.
Book Synopsis Protein Physics by : Alexei V. Finkelstein
Download or read book Protein Physics written by Alexei V. Finkelstein and published by Elsevier. This book was released on 2016-06-22 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Physics: A Course of Lectures covers the most general problems of protein structure, folding and function. It describes key experimental facts and introduces concepts and theories, dealing with fibrous, membrane, and water-soluble globular proteins, in both their native and denatured states. The book systematically summarizes and presents the results of several decades of worldwide fundamental research on protein physics, structure, and folding, describing many physical models that help readers make estimates and predictions of physical processes that occur in proteins. New to this revised edition is the inclusion of novel information on amyloid aggregation, natively disordered proteins, protein folding in vivo, protein motors, misfolding, chameleon proteins, advances in protein engineering & design, and advances in the modeling of protein folding. Further, the book provides problems with solutions, many new and updated references, and physical and mathematical appendices. In addition, new figures (including stereo drawings, with a special appendix showing how to use them) are added, making this an ideal resource for graduate and advanced undergraduate students and researchers in academia in the fields of biophysics, physics, biochemistry, biologists, biotechnology, and chemistry. - Fully revised and expanded new edition based on the latest research developments in protein physics - Written by the world's top expert in the field - Deals with fibrous, membrane, and water-soluble globular proteins, in both their native and denatured states - Summarizes, in a systematic form, the results of several decades of worldwide fundamental research on protein physics and their structure and folding - Examines experimental data on protein structure in the post-genome era
Book Synopsis Towards Unravelling Biological Mechanisms Behind Radiation-induced Oral Mucositis Via Mass Spectrometry-based Proteomics by : Prabal Subedi
Download or read book Towards Unravelling Biological Mechanisms Behind Radiation-induced Oral Mucositis Via Mass Spectrometry-based Proteomics written by Prabal Subedi and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteomics written by Tsz Kwong Man and published by BoD – Books on Demand. This book was released on 2012-02-10 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical research has entered a new era of characterizing a disease or a protein on a global scale. In the post-genomic era, Proteomics now plays an increasingly important role in dissecting molecular functions of proteins and discovering biomarkers in human diseases. Mass spectrometry, two-dimensional gel electrophoresis, and high-density antibody and protein arrays are some of the most commonly used methods in the Proteomics field. This book covers four important and diverse areas of current proteomic research: Proteomic Discovery of Disease Biomarkers, Proteomic Analysis of Protein Functions, Proteomic Approaches to Dissecting Disease Processes, and Organelles and Secretome Proteomics. We believe that clinicians, students and laboratory researchers who are interested in Proteomics and its applications in the biomedical field will find this book useful and enlightening. The use of proteomic methods in studying proteins in various human diseases has become an essential part of biomedical research.
Book Synopsis Mass Spectrometry-based Proteomics Analysis of Secreted Proteins by : Emmanuel K Cudjoe (Jr)
Download or read book Mass Spectrometry-based Proteomics Analysis of Secreted Proteins written by Emmanuel K Cudjoe (Jr) and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Secreted proteins play important roles in many cellular functions and molecular processes. Because secreted proteins potentially enter the blood stream, they can serve as valuable measures of health and disease useful for disease diagnosis and prognosis, therapeutic target identification, and patient stratification in personalized medicine. Consequently, significant interest exists in secreted protein analysis within complex biospecimens, particularly blood but significant bioanalytical challenges including the wide protein dynamic range>10 orders of magnitude remain. The cellular secretome therefore represents a viable alternative to direct biomarker discovery in biofluids. Finally, cellular systems are amenable to labeling for the production of intact stable isotope labeled (SIL) proteins that can be used as global internal standards for quantitative proteomics. In this dissertation, two secretome-focused studies were undertaken. The first study involving candidate biomarker discovery in radiation-induced autophagy utilized the p53-null and inducible H1299 non-small cell lung cancer (NSCLC) secretome. The study identified 364 secreted proteins that were mainly associated with exosomes (N=224) and chaperone activity (N=21). CHGB and SCG2 were identified as potential population-based biomarkers (for patient stratification) due to their consistent overexpression in p53-null H1299 cell secretomes compared to p53-wt cells before and after radiation. FAM3C, CANX, EIF5A, GPI, and TXNRD1 were identified as candidate biomarkers for patient prognosis following radiotherapy due to their differential expression only in response to radiation treatment. In the second study, a comprehensive glycoproteomics characterization of the SILAC-labeled HepG2 secretome was undertaken. 1635 SIL proteins, 492 of which were major plasma proteins including 192 cancer biomarkers were identified with high sequence coverage spanning six orders of magnitude. EDTA plasma spiked with the SIL secretomes yielded 63 proteins that were quantified with H/L ratios in all samples out of 1405 total proteins identified. Additionally, LC-M.S./MS analysis of the Con A and WGA enriched 72h secretome:plasma sample afforded an opportunity to clearly distinguish between glycoproteins in plasma and the HepG2 secretome that share/differ in N-glycan structures. Collectively, the two studies reveal the suitability of the H1299 cancer cell secretome as an experimental model for biomarker studies and support the HepG2 secretome as a viable platform for producing SIL glycoproteins.
