Sub Cellular Proteomics

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Sub-cellular Proteomics

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Author : Harvey Millar
Publisher : Frontiers E-books
ISBN 13 : 2889193020
Total Pages : 255 pages
Book Rating : 4.8/5 (891 download)

Book Synopsis Sub-cellular Proteomics by : Harvey Millar

Download or read book Sub-cellular Proteomics written by Harvey Millar and published by Frontiers E-books. This book was released on 2015-01-05 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whilst significant advances have been made in whole organismal proteomics approaches, many researchers still rely on combinations of tissue selection and subcellular prefractionation methods to reduce the complexity of protein extracts from plants prior to proteomic analysis. Often this will allow identification of many lower abundance proteins of the target proteome and it may involve the selection of specific organs, cell types or the isolation of specific subcellular components. These subcellular proteomes provide insight into functions following various treatments and also contribute to the wider understanding of the entire organismal proteome by cataloguing a series of sub-proteome contents. The aim of this Research Topic is to bring together knowledge of sub cellular components in different plant species to provide a basis for accelerated research. It aims to provide a mini-review for each proposed section that summarizes the current understanding of a particular proteome, with the anticipation that every 5 - 10 years we can update these definitive publications.

Subcellular Proteomics

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Author : Eric Bertrand
Publisher : Springer Science & Business Media
ISBN 13 : 1402059434
Total Pages : 390 pages
Book Rating : 4.4/5 (2 download)

Book Synopsis Subcellular Proteomics by : Eric Bertrand

Download or read book Subcellular Proteomics written by Eric Bertrand and published by Springer Science & Business Media. This book was released on 2007-08-29 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.

Advances in Chemical Proteomics

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Author : Xudong Yao
Publisher : Elsevier
ISBN 13 : 012821435X
Total Pages : 270 pages
Book Rating : 4.1/5 (282 download)

Book Synopsis Advances in Chemical Proteomics by : Xudong Yao

Download or read book Advances in Chemical Proteomics written by Xudong Yao and published by Elsevier. This book was released on 2021-10-22 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Chemical Proteomics provides essential concepts and recent applications on probes, tool compounds and concepts for chemical proteomics and then moves on to applications, including solid-phase reagents, fragment screening, designer compounds and protein lipidation. As the second volume in the Developments in Organic Chemistry series, each chapter is written by experts in the field. Users will find this to be a valuable reference for organic chemists and chemical biologists who are interested in developing tool compounds and reagents to measure and interrogate proteome, develop drug leads, and measure off-target effects and drug toxicity. Analytical chemists who are interested in better understanding organic chemistry behind commonly used reagents for quantitative proteomics and tools compounds in the emerging field of chemical proteomics will also benefit from this comprehensive resource on the topics presented. Provides an ideal, introductory book to chemical proteomics for organic chemists, pharmaceutical chemists and chemical biologists Includes advanced, recent applications and reviews in chemical proteomics Presents valuable work by a global team of experts from the field of proteomics

Quantitative Proteomics

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Author : Claire E Eyers
Publisher : Royal Society of Chemistry
ISBN 13 : 1849738084
Total Pages : 391 pages
Book Rating : 4.8/5 (497 download)

Book Synopsis Quantitative Proteomics by : Claire E Eyers

Download or read book Quantitative Proteomics written by Claire E Eyers and published by Royal Society of Chemistry. This book was released on 2014 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a component of post-genome science, the field of proteomics has assumed great prominence in recent years. Whereas quantitative analyses focussed initially on relative quantification, a greater emphasis is now placed on absolute quantification and consideration of proteome dynamics. Coverage of the topic of quantitative proteomics requires consideration both of the analytical fundamentals of quantitative mass spectrometry and the specific demands of the problem being addressed. Quantitative Proteomics aims to outline the state of the art in mass spectrometry-based quantitative proteomics, describing recent advances and current limitations in the instrumentation used, together with the various methods employed for generating high quality data. Details on both strategies describing how stable isotope labelling can be applied and methods for performing quantitative analysis of proteins in a label-free manner are given. The utility of these strategies to understanding cellular protein dynamics are then exemplified with chapters looking at spatial proteomics, dynamics of protein function as determined by quantifying changes in protein post-translational modification and protein turnover. Finally, a key application of these techniques to biomarker discovery and validation is presented, together with the rapidly developing area of quantitative analysis of protein-based foodstuffs. This exemplary book is essential reading for analytical and biological mass spectrometrists working in proteomics research, as well as those undertaking either fundamental or clinical-based investigations with an interest in understanding protein dynamics and/or biomarker assessment.

Organelle Proteomics

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Author : Delphine Pflieger
Publisher : Humana Press
ISBN 13 : 9781617377723
Total Pages : 0 pages
Book Rating : 4.3/5 (777 download)

Book Synopsis Organelle Proteomics by : Delphine Pflieger

Download or read book Organelle Proteomics written by Delphine Pflieger and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to examine organelle proteomics in depth. It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

Plant Proteomics

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Author : Jozef Samaj
Publisher : Springer Science & Business Media
ISBN 13 : 3540726179
Total Pages : 369 pages
Book Rating : 4.5/5 (47 download)

Book Synopsis Plant Proteomics by : Jozef Samaj

Download or read book Plant Proteomics written by Jozef Samaj and published by Springer Science & Business Media. This book was released on 2007-09-09 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Proteomics highlights rapid progress in this field, with emphasis on recent work in model plant species, sub-cellular organelles, and specific aspects of the plant life cycle such as signaling, reproduction and stress physiology. Several chapters present a detailed look at diverse integrated approaches, including advanced proteomic techniques combined with functional genomics, bioinformatics, metabolomics and molecular cell biology, making this book a valuable resource for a broad spectrum of readers.

Difference Gel Electrophoresis

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Author : Kay Ohlendieck
Publisher : Springer Nature
ISBN 13 : 1071628313
Total Pages : 477 pages
Book Rating : 4.0/5 (716 download)

Book Synopsis Difference Gel Electrophoresis by : Kay Ohlendieck

Download or read book Difference Gel Electrophoresis written by Kay Ohlendieck and published by Springer Nature. This book was released on 2022-11-15 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Plant Proteomics

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Author : Jesus V Jorrin-Novo
Publisher : Humana
ISBN 13 : 9781071605301
Total Pages : 417 pages
Book Rating : 4.6/5 (53 download)

Book Synopsis Plant Proteomics by : Jesus V Jorrin-Novo

Download or read book Plant Proteomics written by Jesus V Jorrin-Novo and published by Humana. This book was released on 2021-06-11 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mass Spectrometry-Based Chemical Proteomics

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Author : W. Andy Tao
Publisher : John Wiley & Sons
ISBN 13 : 1118970217
Total Pages : 448 pages
Book Rating : 4.1/5 (189 download)

Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao

Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Proteomic Biology Using LC/MS

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Author : Nobuhiro Takahashi
Publisher : John Wiley & Sons
ISBN 13 : 0470149647
Total Pages : 275 pages
Book Rating : 4.4/5 (71 download)

Book Synopsis Proteomic Biology Using LC/MS by : Nobuhiro Takahashi

Download or read book Proteomic Biology Using LC/MS written by Nobuhiro Takahashi and published by John Wiley & Sons. This book was released on 2007-08-31 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is one of the first books to focus on the dynamic aspect of proteomes. The book introduces proteomics to the newcomer, reviews the theoretical aspects of proteomics and its state-of-the art technologies, along with a number of biological applications using "classical" proteomic technology. The book also presents a new concept, the Dynamome, or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events.

2-D Proteome Analysis Protocols

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Author : Andrew J. Link
Publisher : Springer Science & Business Media
ISBN 13 : 1592595847
Total Pages : 585 pages
Book Rating : 4.5/5 (925 download)

Book Synopsis 2-D Proteome Analysis Protocols by : Andrew J. Link

Download or read book 2-D Proteome Analysis Protocols written by Andrew J. Link and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

Molecular Biology of The Cell

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Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

Book Synopsis Molecular Biology of The Cell by : Bruce Alberts

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Proteomics in Biomedicine and Pharmacology

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Author :
Publisher : Academic Press
ISBN 13 : 0128005955
Total Pages : 376 pages
Book Rating : 4.1/5 (28 download)

Book Synopsis Proteomics in Biomedicine and Pharmacology by :

Download or read book Proteomics in Biomedicine and Pharmacology written by and published by Academic Press. This book was released on 2014-07-03 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 95 of Advances in Protein Chemistry and Structural Biology focuses on advances in proteomic techniques and their application in biomedicine and pharmacology. This volume describes in detail the applications of these techniques in studying a number of diseases and specific protein modifications and profiles, including phosphoproteomics, glycoproteomics, and more. Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology series is the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics. Describes advances in application of powerful techniques in translational research (biomedicine and pharmacology) Chapters are written by authorities in their field Targeted to a wide audience of researchers, specialists, and students The information provided in the volume is well supported by a number of high quality illustrations, figures, and tables

Neuroproteomics

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Author : Ka Wan Li
Publisher : Humana
ISBN 13 : 9781493996612
Total Pages : 218 pages
Book Rating : 4.9/5 (966 download)

Book Synopsis Neuroproteomics by : Ka Wan Li

Download or read book Neuroproteomics written by Ka Wan Li and published by Humana. This book was released on 2019-08-06 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.

Proteogenomics

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Author : Ákos Végvári
Publisher : Springer
ISBN 13 : 3319423169
Total Pages : 184 pages
Book Rating : 4.3/5 (194 download)

Book Synopsis Proteogenomics by : Ákos Végvári

Download or read book Proteogenomics written by Ákos Végvári and published by Springer. This book was released on 2016-09-29 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights key technologies and identifies areas for further development in proteogenomics. The utility and usefulness of very large Omics data sets (Next Gen Sequencing of DNA, RNA-seq, ribosome profiling, mass spectrometry- and antibody-based proteomics) is discussed and opportunities and challenges of related bioinformatics applications are outlined. The reader will be able to appreciate the interdisciplinary nature of the continuously evolving area of proteogenomics, which has already grown beyond its original concept of verifying gene annotations by proteomics. The chapters presented in this book are arranged to offer a general overview, rather than to provide detailed descriptions of technologies. The selected applications will provide useful insight into the level of detail that can be obtained in relation to certain diseases areas, including cancer biology and personalized medicine. The readers will find that each chapter delivers a comprehensive approach to proteogenomics, each from the point of view of a specific application. Research scientists interested in innovative processes that can offer a unique and at the same time a more complete access to technological developments and concepts that in turn can contribute to a better understand biological functions should read this book.

Proteomics of Subcellular Systems

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Author : Victor Darley Usmar
Publisher :
ISBN 13 : 9781588292384
Total Pages : pages
Book Rating : 4.2/5 (923 download)

Book Synopsis Proteomics of Subcellular Systems by : Victor Darley Usmar

Download or read book Proteomics of Subcellular Systems written by Victor Darley Usmar and published by . This book was released on 1999-05-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Proteomic Profiling

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Author : Anton Posch
Publisher :
ISBN 13 : 9781071611883
Total Pages : pages
Book Rating : 4.6/5 (118 download)

Book Synopsis Proteomic Profiling by : Anton Posch

Download or read book Proteomic Profiling written by Anton Posch and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: