Quantitative Surface And Sub Surface Fluorescence Spatial Frequency Domain Imaging For The Enhanced Resection Of Glioma

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Quantitative Surface and Sub-Surface Fluorescence Spatial Frequency Domain Imaging for the Enhanced Resection of Glioma

Author : Mira Sibai
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)

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Book Synopsis Quantitative Surface and Sub-Surface Fluorescence Spatial Frequency Domain Imaging for the Enhanced Resection of Glioma by : Mira Sibai

Download or read book Quantitative Surface and Sub-Surface Fluorescence Spatial Frequency Domain Imaging for the Enhanced Resection of Glioma written by Mira Sibai and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The extent of resection is now considered a significant prognostic factor; yet, safe complete resection is achieved in only 30% glioma patients. The challenge of surgery arises from the diffusive nature of gliomas known to infiltrate normal parenchyma beyond the resection cavity, which are left undetected. To better visualize glioma, intra-operative fluorescence-guided resection (FGR) has been a practical solution, providing real-time tumor contrast. In its most widely used form, FGR is mediated by the preferential overproduction of the fluorophore protoporphyrin IX (PpIX) in malignant tissue after an oral dose of its precursor 5-Aminolevulinic Acid (ALA). ALA-PpIX-FGR has been shown to significantly increase extent of resection. However, the visual assessment and the variable intrinsic optical attenuation of tissue limit this technique to delineating only high-grade tumors that display strong fluorescence. To this end, the work described in this thesis outlines the development of intraoperative quantitative fluorescence imaging (qFI) to quantify tissue fluorescence from superficial and from sub-surface tumors, by exploiting PpIX's secondary absorption peak at 635 nm. Both developments were facilitated by enabling the quantitative optical-property-mapping and depth-resolving capabilities of a technique called Spatial Frequency Domain Imaging (SFDI). SFDI is a unique form of diffuse optical imaging. Light is spatially modulated at predetermined spatial frequencies and spatially-resolved diffuse reflectance images are collected. Recovering 2D maps of tissue optical properties is a prerequisite for both qFI and depth imaging, thus, a custom-built SFDI system was developed and optimized for both techniques. By determining tissue optical properties at both the absorption and emission wavelengths of PpIX, the raw hyperspectral fluorescence images are converted to 2D maps of PpIX concentration, [PpIX], after applying a quantitative fluorescent model. qFI is now being integrated on a surgical microscope for future clinical trials. While SFD-enabled-qFI proved to be a practical extension to the quantitative probe, both are still blind to sub-surface tumors, due to the limited penetration depth of the excitation light. This limitation prompted the development of quantitative sub-surface fluorescence imaging, where tumor depth and [PpIX] can be recovered to assist the surgeon in making an informed decision on whether to continue resection.

Quantitative and Depth-resolved Fluorescence Guidance for the Resection of Glioma

Author : Anthony Taywon Kim
Publisher :
ISBN 13 : 9780494721858
Total Pages : 346 pages
Book Rating : 4.7/5 (218 download)

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Book Synopsis Quantitative and Depth-resolved Fluorescence Guidance for the Resection of Glioma by : Anthony Taywon Kim

Download or read book Quantitative and Depth-resolved Fluorescence Guidance for the Resection of Glioma written by Anthony Taywon Kim and published by . This book was released on 2010 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The clinical management of glioma remains a challenge. The prognosis is poor--for glioblastoma multiforme, the most virulent of these brain cancers, survival is only ∼1 year. Surgical resection of the tumor is the first line of defense. Several studies demonstrate a survival advantage in patients who undergo near-complete tumor resection; however, achieving complete resection is limited by the difficulty of visualizing residual tumor after de-bulking. Intraoperative fluorescence guidance is a promising candidate to better visualize residual tumor. The most clinically developed form uses protoporphyrin IX fluorescence, the precursor to heme in its biosynthesis which preferentially accumulates in tumor cells after the administration of 5-aminolevulinic acid. Challenges remain in quantitatively assessing the fluorescence to reduce variability of outcome and improve tumor detection specificity, and in observing sub-surface tumor fluorescence. To these ends, this work outlines the development of intraoperative techniques to 1) quantify tissue fluorescence using a handheld fiberoptic probe and 2) improve detection by reconstructing the depth-resolved fluorescence topography of sub-surface tumor.As a critical component to achieve these objectives, a technique to measure the tissue optical properties was developed. This technique used diffuse reflectance measurements mediated by a handheld fiberoptic probe to derive the tissue optical properties. The handheld fiberoptic probe was further developed to include fluorescence spectroscopy. A novel algorithm to combine the fluorescence measurement and the tissue optical properties was derived in order to extract the quantitative fluorescence spectrum, i.e. fluorescence without confounding effects of tissue optical properties. The concentration of fluorescent tumor biomarker can then be extracted. The quantitative fluorescence work culminated in deployment of the fiberoptic probe in clinical trials for the resection of intracranial tumors. The quantitative fluorescence probe out-performed a state-of-the-art fluorescence surgical microscope for a broad range of brain tumor pathologies.A novel technique for depth-resolved fluorescence detection was developed utilizing multi-excitation fluorescence imaging. An algorithm to extract depth information from the multi-excitation images was derived, with validation in phantoms and a rat brain tumor model. This demonstrates the potential for depth-resolved fluorescence imaging, which there is a clear need for in tumor resection guidance.

Glioma Imaging

Author : Whitney B. Pope
Publisher : Springer Nature
ISBN 13 : 3030273598
Total Pages : 286 pages
Book Rating : 4.0/5 (32 download)

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Book Synopsis Glioma Imaging by : Whitney B. Pope

Download or read book Glioma Imaging written by Whitney B. Pope and published by Springer Nature. This book was released on 2019-11-11 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers physiologic, metabolic and molecular imaging for gliomas. Gliomas are the most common primary brain tumors. Imaging is critical for glioma management because of its ability to noninvasively define the anatomic location and extent of disease. While conventional MRI is used to guide current treatments, multiple studies suggest molecular features of gliomas may be identified with noninvasive imaging, including physiologic MRI and amino acid positron emission tomography (PET). These advanced imaging techniques have the promise to help elucidate underlying tumor biology and provide important information that could be integrated into routine clinical practice. The text outlines current clinical practice including common scenarios in which imaging interpretation impacts patient management. Gaps in knowledge and potential areas of advancement based on the application of more experimental imaging techniques will be discussed. In reviewing this book, readers will learn: current standard imaging methodologies used in clinical practice for patients undergoing treatment for glioma and the implications of emerging treatment modalities including immunotherapy the theoretical basis for advanced imaging techniques including diffusion and perfusion MRI, MR spectroscopy, CEST and amino acid PET the relationship between imaging and molecular/genomic glioma features incorporated in the WHO 2016 classification update and the potential application of machine learning about the recently adopted and FDA approved standard brain tumor protocol for multicenter drug trials of the gaps in knowledge that impede optimal patient management and the cutting edge imaging techniques that could address these deficits

Localization of Subsurface Fluorescent Lesions Using Surface Spectral Measurements

Author : Kolbein K. Kolste
Publisher :
ISBN 13 :
Total Pages : 262 pages
Book Rating : 4.:/5 (931 download)

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Book Synopsis Localization of Subsurface Fluorescent Lesions Using Surface Spectral Measurements by : Kolbein K. Kolste

Download or read book Localization of Subsurface Fluorescent Lesions Using Surface Spectral Measurements written by Kolbein K. Kolste and published by . This book was released on 2015 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: In neurosurgical tumor resection, the current standard protocol is to use pre-operative MR image combined with tracking equipment to provide a map for intraoperative guidance. This technology has improved surgical outcomes, but is limited by deformation of the brain during the surgery. A potential supplement to image-guidance is fluorescence-guidance. To date, ALA is being researched as a technique to guide tumor resection by inducing the accumulation of the endogenous fluorophor PpIX. Most research has focused on the use of blue light excitation of PpIX to visual the tumor. However, due to the high attenuation of blue light by in vivo chromophores, such as oxy- and deoxy- hemoglobin, the source of collected fluorescence emissions is confined to the top layer of cells, and the signal is subject to masking by blood on the surface of the surgical field of view. This issue is particularly a problem at the end of the resection, when the surgeon is evaluating the margin for remaining tumor, but the blue-signal is insensitive to residual tumor that may be located several millimeters beneath the surface. PpIX has an absorption band in the near infrared (NIR), where the absorption due to blood is orders of magnitude lower, enabling the excitation of a fluorophore at depth. In this work, we created a hyperspectral imaging system that attaches to a neurosurgical microscope and is capable of detecting PpIX fluorescence that has been excited at 635 nm. We utilize a dual-waveband technique from the hyperspectral to estimate depth of fluorescence origin and characterize the inherent limitations of the estimated depth. One of the major benefits of this technique is that the estimation is independent of the concentration and size of the fluorophore. This is first demonstrated in phantom studies, where the depths of multiple separate inclusions at various depths are accurately estimated. The technique is verified in animal tumor models and translated into the clinical theater, with pilot data showing the first estimation of depth of subsurface tumors during intracranial tumor resection.

Absorption, Fluorescence, and Dynamic Scattering Contrast in Spatial Frequency Domain Imaging (SFDI)

Author : Amaan Mazhar
Publisher :
ISBN 13 : 9781124208541
Total Pages : 105 pages
Book Rating : 4.2/5 (85 download)

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Book Synopsis Absorption, Fluorescence, and Dynamic Scattering Contrast in Spatial Frequency Domain Imaging (SFDI) by : Amaan Mazhar

Download or read book Absorption, Fluorescence, and Dynamic Scattering Contrast in Spatial Frequency Domain Imaging (SFDI) written by Amaan Mazhar and published by . This book was released on 2010 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative characterization of tissue structure and function is a challenge due to structural heterogeneity and functional diversity across multiple spatial scales. Optical technologies are an inexpensive and safe method to visualize tissue at all spatial scales. Spatial Frequency Domain Imaging (SFDI) is a non-invasive, scan-free, and scalable field of view technology used to characterize tissue. Three SFDI instruments were developed to explore optical contrast mechanisms due to absorption, dynamic scattering, and fluorescence in tissue. For each platform, spectral and spatial frequency parameters were investigated in phantoms and in vivo to measure absorption for fast hemodynamics, fluorescence for accurate margin delineation, and dynamic light scattering for tissue perfusion. Based on these studies, a large field of view SFDI system was designed and constructed for intra-operative imaging. System validation was performed in pre-clinical models for free-flap measurement and sentinel lymph node mapping, and in a human subject during reconstructive surgery.

Angiogenesis Assays

Author : Carolyn A. Staton
Publisher : John Wiley & Sons
ISBN 13 : 047002934X
Total Pages : 410 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Angiogenesis Assays by : Carolyn A. Staton

Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.

Physical Properties of Tissues

Author : Francis Duck
Publisher : Academic Press
ISBN 13 : 1483288420
Total Pages : 357 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Physical Properties of Tissues by : Francis Duck

Download or read book Physical Properties of Tissues written by Francis Duck and published by Academic Press. This book was released on 2013-10-22 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique reference book describes quantitatively the measured and predicted values of all the physical properties of mammalian tissue. Reported measurements are thoroughly documented and are complemented by a range of empirical mathematical models which describe the observed physical behavior of tissue.**Intended as a broad-ranging reference, this volume gives the bioengineer, physicist, radiologist, or physiologist access to a literature which may not be known in detail. It will also be of value for those concerned with the study of a range of environmental radiation hazards. Most extensive compilation of values of physical properties of tissue**Presents data for thermal, optical, ultrasonic, mechanical, x-ray, electrical, and magnetic resonance properties**Comprehensive bibliography

Fluorescence Correlation Spectroscopy

Author : R. Rigler
Publisher : Springer Science & Business Media
ISBN 13 : 3642595421
Total Pages : 503 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Fluorescence Correlation Spectroscopy by : R. Rigler

Download or read book Fluorescence Correlation Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.

Intraoperative Imaging

Author : M. Necmettin Pamir
Publisher : Springer Science & Business Media
ISBN 13 : 3211996516
Total Pages : 253 pages
Book Rating : 4.2/5 (119 download)

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Book Synopsis Intraoperative Imaging by : M. Necmettin Pamir

Download or read book Intraoperative Imaging written by M. Necmettin Pamir and published by Springer Science & Business Media. This book was released on 2010-10-20 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intraoperative imaging technologies have taken an ever-increasing role in the daily practice of neurosurgeons and the increasing attention and interest necessitated international interaction and collaboration. The Intraoperative Imaging Society was formed in 2007. This book brings together highlights from the second meeting of the Intraoperative Imaging Society, which took place in Istanbul-Turkey from June 14 to 17, 2009. Included within the contents of the book is an overview of the emergence and development of the intraoperative imaging technology as well as a glimpse on where the technology is heading. This is followed by in detail coverage of intraoperative MRI technology and sections on intraoperative CT and ultrasonography. There are also sections on multimodality integration, intraoperative robotics and other intraoperative technologies. We believe that this book will provide an up-to date and comprehensive general overview of the current intraoperative imaging technology as well as detailed discussions on individual techniques and clinical results.

Imaging in Photodynamic Therapy

Author : Michael R. Hamblin
Publisher : CRC Press
ISBN 13 : 1315278162
Total Pages : 502 pages
Book Rating : 4.3/5 (152 download)

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Book Synopsis Imaging in Photodynamic Therapy by : Michael R. Hamblin

Download or read book Imaging in Photodynamic Therapy written by Michael R. Hamblin and published by CRC Press. This book was released on 2017-02-03 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the broad field of cellular, molecular, preclinical, and clinical imaging either associated with or combined with photodynamic therapy (PDT). It showcases how this approach is used clinically for cancer, infections, and diseases characterized by unwanted tissue such as atherosclerosis or blindness. Because the photosensitizers are also fluorescent, the book also addresses various imaging systems such as confocal microscopy and small animal imaging systems, and highlights how they have been used to follow and optimize treatment, and to answer important mechanistic questions. Chapters also discuss how imaging has made important contributions to clinical outcomes in skin, bladder, and brain cancers, as well as in the development of theranostic agents for detection and treatment of disease. This book provides a resource for physicians and research scientists in cell biology, microscopy, optics, molecular imaging, oncology, and drug discovery.

Guide to Best Practices for Ocean Acidification Research and Data Reporting

Author :
Publisher :
ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.3/5 (21 download)

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Book Synopsis Guide to Best Practices for Ocean Acidification Research and Data Reporting by :

Download or read book Guide to Best Practices for Ocean Acidification Research and Data Reporting written by and published by . This book was released on 2011 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Small Animal Imaging

Author : Fabian Kiessling
Publisher : Springer
ISBN 13 : 3319422022
Total Pages : 871 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Small Animal Imaging by : Fabian Kiessling

Download or read book Small Animal Imaging written by Fabian Kiessling and published by Springer. This book was released on 2017-05-22 with total page 871 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook is a practical guide to the use of small animal imaging in preclinical research that will assist in the choice of imaging modality and contrast agent and in study design, experimental setup, and data evaluation. All established imaging modalities are discussed in detail, with the assistance of numerous informative illustrations. While the focus of the new edition remains on practical basics, it has been updated to encompass a variety of emerging imaging modalities, methods, and applications. Additional useful hints are also supplied on the installation of a small animal unit, study planning, animal handling, and cost-effective performance of small animal imaging. Cross-calibration methods and data postprocessing are considered in depth. This new edition of Small Animal Imaging will be an invaluable aid for researchers, students, and technicians involved in research into and applications of small animal imaging.

Anion Sensing

Author : Eric V. Anslyn
Publisher : Springer Science & Business Media
ISBN 13 : 9783540232476
Total Pages : 252 pages
Book Rating : 4.2/5 (324 download)

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Book Synopsis Anion Sensing by : Eric V. Anslyn

Download or read book Anion Sensing written by Eric V. Anslyn and published by Springer Science & Business Media. This book was released on 2005-05-06 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: with contributions by numerous experts

Human Health and Performance Risks of Space Exploration Missions

Author : Jancy C. McPhee
Publisher : U. S. National Aeronautics & Space Administration
ISBN 13 :
Total Pages : 396 pages
Book Rating : 4.3/5 ( download)

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Book Synopsis Human Health and Performance Risks of Space Exploration Missions by : Jancy C. McPhee

Download or read book Human Health and Performance Risks of Space Exploration Missions written by Jancy C. McPhee and published by U. S. National Aeronautics & Space Administration. This book was released on 2009 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fundamentals of Fluorescence Imaging

Author : Guy Cox
Publisher : CRC Press
ISBN 13 : 1351129384
Total Pages : 430 pages
Book Rating : 4.3/5 (511 download)

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Book Synopsis Fundamentals of Fluorescence Imaging by : Guy Cox

Download or read book Fundamentals of Fluorescence Imaging written by Guy Cox and published by CRC Press. This book was released on 2019-04-23 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Diffuse Low-Grade Gliomas in Adults

Author : Hugues Duffau
Publisher : Springer Science & Business Media
ISBN 13 : 1447122135
Total Pages : 498 pages
Book Rating : 4.4/5 (471 download)

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Book Synopsis Diffuse Low-Grade Gliomas in Adults by : Hugues Duffau

Download or read book Diffuse Low-Grade Gliomas in Adults written by Hugues Duffau and published by Springer Science & Business Media. This book was released on 2013-03-19 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest research pertaining to the diagnosis, therapy and management of diffuse low-grade gliomas (DLGG) in adults, with a particular focus on the path towards individualised therapy for this kind of tumour. Recent research on the natural history of DLGGs and their interaction with the brain has led to new diagnostic and therapeutic strategies which increase survival and quality of life of the patient, and these methods are described in this book.

In Vivo Fluorescence Imaging

Author : Mingfeng Bai
Publisher : Humana
ISBN 13 : 9781493937196
Total Pages : 0 pages
Book Rating : 4.9/5 (371 download)

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Book Synopsis In Vivo Fluorescence Imaging by : Mingfeng Bai

Download or read book In Vivo Fluorescence Imaging written by Mingfeng Bai and published by Humana. This book was released on 2016-06-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking, image-guided treatment, and fluorescence imaging in the second NIR window, as well as protocols for evaluation methods before and after in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerous fields who wish to become more familiar with in vivo fluorescence imaging techniques.