Protoplast

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Protoplast Technology

Author : Kan Wang
Publisher : Humana
ISBN 13 : 9781071621660
Total Pages : 0 pages
Book Rating : 4.6/5 (216 download)

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Book Synopsis Protoplast Technology by : Kan Wang

Download or read book Protoplast Technology written by Kan Wang and published by Humana. This book was released on 2023-03-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

Tissue Culture of Trees

Author : John H. Dodds
Publisher : Springer Science & Business Media
ISBN 13 : 1468466917
Total Pages : 157 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Tissue Culture of Trees by : John H. Dodds

Download or read book Tissue Culture of Trees written by John H. Dodds and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually described as an un organised proliferating mass of tissue. Although callus cultures have a great deal of potential in the biotechnological aspects of tissue culture, i.e. secondary product formation, they are not very suitable for plant propagation. The key reason for their unsuitability is that genetic aber rations occur during mitotic divisions in callus growth (D'amato.l965). The aberrations can be of a major type, such as aneuploidy or endo reduplication. It follows therefore that the genetic status of the re generated plants is different from that of the parent type. In general terms this genetic instability is undesirable, but there are occasions when a callus stage can be purposely included to diversify the genetic base of the crop.

Protoplast Fusion

Author : Y. Y. Gleba
Publisher : Springer
ISBN 13 : 9783642822476
Total Pages : 0 pages
Book Rating : 4.8/5 (224 download)

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Book Synopsis Protoplast Fusion by : Y. Y. Gleba

Download or read book Protoplast Fusion written by Y. Y. Gleba and published by Springer. This book was released on 2011-12-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.

Introduction to Plant Biotechnology

Author : H. S. Chawla
Publisher : Science Publishers
ISBN 13 : 9781578082285
Total Pages : 568 pages
Book Rating : 4.0/5 (822 download)

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Book Synopsis Introduction to Plant Biotechnology by : H. S. Chawla

Download or read book Introduction to Plant Biotechnology written by H. S. Chawla and published by Science Publishers. This book was released on 2002 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.

Fungal Protoplasts

Author : J. Peberdy
Publisher : CRC Press
ISBN 13 : 9780824771126
Total Pages : 378 pages
Book Rating : 4.7/5 (711 download)

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Book Synopsis Fungal Protoplasts by : J. Peberdy

Download or read book Fungal Protoplasts written by J. Peberdy and published by CRC Press. This book was released on 1985-02-20 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.

PLANT PROTOPLASTS

Author : Fowke
Publisher : Boca Raton, Fla. : CRC Press
ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis PLANT PROTOPLASTS by : Fowke

Download or read book PLANT PROTOPLASTS written by Fowke and published by Boca Raton, Fla. : CRC Press. This book was released on 1985-10-04 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protoplast isolation and culture; Regeneration of plants; Protoplasts for studies of cell organelles; Protoplast fusion, Plant protoplasts and the cell cycle; Isolated protoplasts as laboratory tools in the study of cell wall deposition; Cytodifferentiation; The use of protoplasts for studies on membrane transport in plants; Protoplasts in virology; Plant protoplasts and genetic variation; Plant mutant isolation via protoplasts; Hybridization by somatic cell fusion; Plant protoplast transformation by Agrobacterium in relation to plant biotechnology.

Plant Protoplasts

Author : Tessa Bengochea
Publisher : Springer Science & Business Media
ISBN 13 : 9400940955
Total Pages : 97 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Plant Protoplasts by : Tessa Bengochea

Download or read book Plant Protoplasts written by Tessa Bengochea and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.

Progress in Plant Protoplast Research

Author : K.J. Puite
Publisher : Springer Science & Business Media
ISBN 13 : 9400927886
Total Pages : 415 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Progress in Plant Protoplast Research by : K.J. Puite

Download or read book Progress in Plant Protoplast Research written by K.J. Puite and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987

Plant Protoplasts and Genetic Engineering V

Author : Professor Dr. Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3662093669
Total Pages : 432 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering V by : Professor Dr. Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering V written by Professor Dr. Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

The Physiological Properties of Plant Protoplasts

Author : Paul-Emile Pilet
Publisher : Springer Science & Business Media
ISBN 13 : 3642701442
Total Pages : 296 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis The Physiological Properties of Plant Protoplasts by : Paul-Emile Pilet

Download or read book The Physiological Properties of Plant Protoplasts written by Paul-Emile Pilet and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea for the p~esent book arose from a 3-day seminar which I organized in March 1984 for young research workers in plant physiology. Participants came from several universities of the French-speaking part of Switzerland and speakers from Basel, Mtinchen, Nottingham, Perpignan, Regensburg, Sheffield, Toulouse, Yale, ZUrich ... and Lausannne. The theme of the seminar was chosen from the range of research fields of our Institute. Meanwhile, feeling it was important to bear in mind that some of our hearers were not specialists in the chosen topic, I wished to pro vide a subject that would be of scientific, methodological and epistemologi cal interest. The critical analysis of the structural and functional characteristics of plant protoplasts exactly met these criteria. There exists ample material for discussion of the techniques of protoplast preparation, methods used in morphological, biological and biochemical studies, and for the comparison of protoplasts with the cells from which they are obtained.

Plant Cell, Tissue and Organ Culture

Author : Oluf Gamborg
Publisher : Springer Science & Business Media
ISBN 13 : 3642790488
Total Pages : 367 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Cell, Tissue and Organ Culture by : Oluf Gamborg

Download or read book Plant Cell, Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Plant Protoplasts

Author : Fowke
Publisher : CRC Press
ISBN 13 : 1351084224
Total Pages : 254 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Plant Protoplasts by : Fowke

Download or read book Plant Protoplasts written by Fowke and published by CRC Press. This book was released on 2018-01-18 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.

Plant Protoplasts

Author : Kenneth L. Giles
Publisher : Elsevier
ISBN 13 : 1483218392
Total Pages : 320 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Plant Protoplasts by : Kenneth L. Giles

Download or read book Plant Protoplasts written by Kenneth L. Giles and published by Elsevier. This book was released on 2013-10-15 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Protoplasts covers the techniques involved with, and uses of, protoplast technology. The book discusses isolation, fusion, and culture of higher plant protoplasts, lower plant protoplasts, and blue-green algal protoplast. The text also describes the production of haploid protoplasts from developing pollen grains; the use of protoplasts in mutant selection schemes, and the development of protoplast systems for use with monocotyledonous plants. The book will be invaluable to plant technologists, botanists, biochemists, research workers as well as advanced students interested in gaining a background knowledge of the field.

Plant Protoplasts and Genetic Engineering VII

Author : Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3662093685
Total Pages : 339 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering VII by : Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering VII written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Twenty-seven chapters deal with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. This volume is of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general plant biotechnology.

Plant Protoplasts and Genetic Engineering II

Author : Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642744540
Total Pages : 516 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering II by : Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering II written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fungal Protoplasts

Author : John F. Peberdy
Publisher : CRC Press
ISBN 13 : 1000110591
Total Pages : 369 pages
Book Rating : 4.0/5 (1 download)

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Book Synopsis Fungal Protoplasts by : John F. Peberdy

Download or read book Fungal Protoplasts written by John F. Peberdy and published by CRC Press. This book was released on 2020-10-28 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.

Plant Protoplasts and Genetic Engineering IV

Author : Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642780377
Total Pages : 406 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering IV by : Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering IV written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.