Proteomics Assisted Discovery Of Novel Proteins In Assembly And Regulation Of Photosystem Ii

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Proteomics Assisted Discovery of Novel Proteins in Assembly and Regulation of Photosystem II

Author : Kimberly Marie Wegener
Publisher :
ISBN 13 :
Total Pages : 227 pages
Book Rating : 4.:/5 (756 download)

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Book Synopsis Proteomics Assisted Discovery of Novel Proteins in Assembly and Regulation of Photosystem II by : Kimberly Marie Wegener

Download or read book Proteomics Assisted Discovery of Novel Proteins in Assembly and Regulation of Photosystem II written by Kimberly Marie Wegener and published by . This book was released on 2010 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria, the only prokaryotes capable of oxygenic photosynthesis, are present in diverse ecological niches and play crucial roles in global carbon and nitrogen cycles. To proliferate in nature, cyanobacteria utilize a host of stress responses to maintain photosynthesis under periodic changes in environmental conditions. Recent advances in proteomic study have enabled a systems-level analysis of cellular functions in many systems. Because proteins are directly responsible for cellular functions, measurements of protein abundances provide significant clues to the modulation of cellular functions during different environmental perturbations. A detailed knowledge of the composition of, as well as the dynamic changes in, the proteome is necessary to gain fundamental insights into such stress responses. Toward this goal, we have performed a large-scale proteomic analysis of the widely studied model cyanobacterium Synechocystis sp. PCC 6803 under 33 different environmental conditions. Photosystem II (PSII) is a large membrane protein complex that performs the water oxidation reactions of the photosynthetic electron transport chain in cyanobacteria, algae, and plants. Subsequently, we also performed an accurate mass tag (AMT) high-sensitivity proteomic analysis of PSII complexes purified from the cyanobacterium Synechocystis sp. PCC 6803. Taken together, these proteomics studies revealed novel information into the function and assembly of Photosystem II. We identified six PSII associated proteins that are encoded by a single operon containing nine genes, slr0144 to slr0152. This operon encodes proteins that are not essential components of the PSII holocomplex but accumulate to high levels in precomplexes lacking any of the lumenal proteins PsbP, PsbQ, or PsbV. Genetic deletion of this operon shows that removal of these protein products does not alter photoautotrophic growth or PSII fluorescence properties. Nonetheless these proteins confer fitness under competition in high light intensities. However, the deletion mutation does result in decreased PSII-mediated oxygen evolution and an altered distribution of the S states of the catalytic Mn cluster. PSII complexes isolated from [delta]slr0144 - slr0152 also show decreased photosynthetic capacity and altered polypeptide composition. These data demonstrate that the proteins encoded by the genes in this operon are necessary for optimal function of PSII and function as accessory proteins during assembly of the PSII complex. Based on these results, we have named the products of the slr0144 - slr0152 operon Pap (photosystem II assembly proteins). Additionally, through this proteomics study, we identified the protein sll1390, which we have named Psb32. To investigate its function, we analyzed subcellular localization of Psb32 and the impact of genetic deletion of the psb32 gene on PSII. Psb32 is an integral membrane protein, primarily located in the thylakoid membranes. Although not required for cell viability, Psb32 protects cells from oxidative stress and additionally confers a selective fitness advantage in mixed culture experiments. Specifically, Psb32 protects PSII from photodamage and accelerates its repair. Thus, we propose that Psb32 plays an important role in minimizing the effect of photoinhibition on PSII. Together, the proteins of the pap operon and Psb32 represent new components in PSII assembly and function.

Assembly of the Photosystem II Membrane-Protein Complex of Oxygenic Photosynthesis

Author : Julian J. Eaton-Rye
Publisher : Frontiers Media SA
ISBN 13 : 2889452336
Total Pages : 317 pages
Book Rating : 4.8/5 (894 download)

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Book Synopsis Assembly of the Photosystem II Membrane-Protein Complex of Oxygenic Photosynthesis by : Julian J. Eaton-Rye

Download or read book Assembly of the Photosystem II Membrane-Protein Complex of Oxygenic Photosynthesis written by Julian J. Eaton-Rye and published by Frontiers Media SA. This book was released on 2017-08-08 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Photosystem II is a 700-kDa membrane-protein super-complex responsible for the light-driven splitting of water in oxygenic photosynthesis. The photosystem is comprised of two 350-kDa complexes each made of 20 different polypeptides and over 80 co-factors. While there have been major advances in understanding the mature structure of this photosystem many key protein factors involved in the assembly of the complex do not appear in the holoenzyme. The mechanism for assembling this super-complex is a very active area of research with newly discovered assembly factors and subcomplexes requiring characterization. Additionally the ability to split water is inseparable from light-induced photodamage that arises from radicals and reactive O2 species generated by Photosystem II chemistry. Consequently, to sustain water splitting, a “self repair” cycle has evolved whereby damaged protein is removed and replaced so as to extend the working life of the complex. Understanding how the biogenesis and repair processes are coordinated is among several important questions that remain to be answered. Other questions include: how and when are the inorganic cofactors inserted during the assembly and repair processes and how are the subcomplexes protected from photodamage during assembly? Evidence has also been obtained for Photosystem II biogenesis centers in cyanobacteria but do these also exist in plants? Do the molecular mechanisms associated with Photosystem II assembly shed fresh light on the assembly of other major energy-transducing complexes such as Photosystem I or the cytochrome b6/f complex or indeed other respiratory complexes? The contributions to this Frontiers in Plant Science Research Topic are likely to reveal new details applicable to the assembly of a range of membrane-protein complexes, including aspects of self-assembly and solar energy conversion that may be applied to artificial photosynthetic systems. In addition, a deeper understanding of Photosystem II assembly — particularly in response to changing environmental conditions — will provide new knowledge underpinning photosynthetic yields which may contribute to improved food production and long-term food security.

Functional Genomics and Evolution of Photosynthetic Systems

Author : Robert Burnap
Publisher : Springer Science & Business Media
ISBN 13 : 9400715331
Total Pages : 426 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Functional Genomics and Evolution of Photosynthetic Systems by : Robert Burnap

Download or read book Functional Genomics and Evolution of Photosynthetic Systems written by Robert Burnap and published by Springer Science & Business Media. This book was released on 2011-09-18 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: New possibilities have been brought about by the stunning number of genomic sequences becoming available for photosynthetic organisms. This new world of whole genome sequence data spans the phyla from photosynthetic microbes to algae to higher plants. These whole genome projects are intrinsically interesting, but also inform the variety of other molecular sequence databases including the recent 'meta-genomic' sequencing efforts that analyze entire communities of organisms. As impressive as they are, are obviously only the beginning of the effort to decipher the biological meaning encoded within them. This book aims to highlight progress in this direction. This book aims toward a genome-level understanding of the structure, function, and evolution of photosynthetic systems and the advantages accrued from the availability of phyletically diverse sets of gene sequences for the major components of the photosynthetic apparatus. While not meant to be fully comprehensive in terms of the topics covered, it does provide detailed views of specific cases and thereby illustrates important new directions that are being taken in this fast-moving field—a field that involves the integration of bioinformatics, molecular biology, physiology, and ecology.

Photosystem I

Author : John H. Golbeck
Publisher : Springer Science & Business Media
ISBN 13 : 1402042566
Total Pages : 744 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Photosystem I by : John H. Golbeck

Download or read book Photosystem I written by John H. Golbeck and published by Springer Science & Business Media. This book was released on 2007-05-20 with total page 744 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes recent advances made in the biophysics, biochemistry, and molecular biology of the enzyme known as Photosystem I, the light-induced plastocyanin: ferredoxin oxidoreductase. The volume provides a unique compilation of chapters that includes information highlighting controversial issues to indicate the frontiers of research and places special emphasis on methodology and practice for new researchers.

Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling

Author : William A. Cramer
Publisher : Springer
ISBN 13 : 9401774811
Total Pages : 763 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling by : William A. Cramer

Download or read book Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling written by William A. Cramer and published by Springer. This book was released on 2016-06-14 with total page 763 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Introduction that describes the origin of cytochrome notation also connects to the history of the field, focusing on research in England in the pre-World War II era. The start of the modern era of studies on structure-function of cytochromes and energy-transducing membrane proteins was marked by the 1988 Nobel Prize in Chemistry, given to J. Deisenhofer, H. Michel, and R. Huber for determination of the crystal structure of the bacterial photosynthetic reaction center. An ab initio logic of presentation in the book discusses the evolution of cytochromes and hemes, followed by theoretical perspectives on electron transfer in proteins and specifically in cytochromes. There is an extensive description of the molecular structures of cytochromes and cytochrome complexes from eukaryotic and prokaryotic sources, bacterial, plant and animal. The presentation of atomic structure information has a major role in these discussions, and makes an important contribution to the broad field of membrane protein structure-function.

Biohydrogen III

Author : Matthias Rogner
Publisher : Elsevier
ISBN 13 : 0080472117
Total Pages : 189 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Biohydrogen III by : Matthias Rogner

Download or read book Biohydrogen III written by Matthias Rogner and published by Elsevier. This book was released on 2004-02-19 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogen is an almost ideal fuel and its wider use will result in an improvement in the environment due to factors including decreased air pollution. Hydrogen is the element of greatest abundance in the universe; however, its production from renewable resources remains a major challenge. The papers presented within this volume enhance and expand upon presentations made at the "Workshop on Biohydrogen 2002". Biohydrogen III evaluates the current status of Biohydrogen research worldwide and consider future research directions. Important research on new fuel opportunities 15 contributions from the world's leading experts

Endocytosis in Plants

Author : Jozef Šamaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642324622
Total Pages : 337 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis Endocytosis in Plants by : Jozef Šamaj

Download or read book Endocytosis in Plants written by Jozef Šamaj and published by Springer Science & Business Media. This book was released on 2012-10-02 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.

Photosynthesis Research Protocols

Author : Robert Carpentier
Publisher : Springer Science & Business Media
ISBN 13 : 1592597998
Total Pages : 341 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Photosynthesis Research Protocols by : Robert Carpentier

Download or read book Photosynthesis Research Protocols written by Robert Carpentier and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Photosynthesis is one of the most important biological phenomena on earth. The conversion of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Photosynthesis is not only at the heart of plant bioenergetics, it is also fundamental to plant prod- tivity and biomass. Photosynthetic carbon fixation and oxygen evolution - rectly intervene in many environmental, including the global atmospheric CO 2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Several biochemical methods of isolation, treatment, and analysis have been developed to fulfill the needs of photosynthesis research. Photosynthesis Research Protocols contains a broad range of general and fundamental me- ods that are commonly used by plant biochemists, physiologists, and mole- lar biologists. This book is thus intended as a source of information for scientists working on any of the multiple aspects of photosynthesis, and should be of great interest to a multidisciplinary field of research involving agric- ture, biochemistry, biotechnology, botany, cell biology, environmental s- ences, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, plant physiology, plant stress, etc.

Plastid Biology

Author : Steven M. Theg
Publisher : Springer
ISBN 13 : 1493911368
Total Pages : 585 pages
Book Rating : 4.4/5 (939 download)

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Book Synopsis Plastid Biology by : Steven M. Theg

Download or read book Plastid Biology written by Steven M. Theg and published by Springer. This book was released on 2014-09-19 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plastids are the sites of conversion of solar energy into the chemical energy usable to sustain life. They are also responsible for the production of the vast majority of the oxygen in the atmosphere. Through these activities they play a unique role in the biosphere, producing two critical products upon which life on Earth depends. It covers in 21 chapters nearly all actively investigated areas of plastid biology, from biosynthesis to function to their uses in biotechnology. The editors have compiled an extensive list of international experts from whom to solicit chapters. As is evident from the suggested Table of Contents, the book will start with a discussion of genetic material and its expression, followed by differentiation and development of different plastid types and internal organization. This is followed by an in depth look at biogenesis and assembly of plastid proteins and protein complexes and then by the important metabolic functions in plastids. The book will end with two chapters discussing the role of plastid biology in protein expression biotechnology and in hydrogen and biofuel production.

Chlorophyll a Fluorescence

Author : G.C. Papageorgiou
Publisher : Springer Science & Business Media
ISBN 13 : 1402032188
Total Pages : 853 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Chlorophyll a Fluorescence by : G.C. Papageorgiou

Download or read book Chlorophyll a Fluorescence written by G.C. Papageorgiou and published by Springer Science & Business Media. This book was released on 2007-11-12 with total page 853 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring quantitatively various processes of photosynthesis. The book explains the mechanisms with which plants defend themselves against environmental stresses (excessive light, extreme temperatures, drought, hyper-osmolarity, heavy metals and UV). It also includes discussion on fluorescence imaging of leaves and cells and the remote sensing of Chl fluorescence from terrestrial, airborne, and satellite bases. The book is intended for use by graduate students, beginning researchers and advanced undergraduates in the areas of integrative plant biology, cellular and molecular biology, plant biology, biochemistry, biophysics, plant physiology, global ecology and agriculture.

Oxygenic Photosynthesis: The Light Reactions

Author : Donald R. Ort
Publisher : Springer Science & Business Media
ISBN 13 : 0306481278
Total Pages : 681 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Oxygenic Photosynthesis: The Light Reactions by : Donald R. Ort

Download or read book Oxygenic Photosynthesis: The Light Reactions written by Donald R. Ort and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 681 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structure and function of the components of the photosynthetic apparatus and the molecular biology of these components have become the dominant themes in advances in our understanding of the light reactions of oxygenic photosynthesis. Oxygenic Photosynthesis: The Light Reactions presents our current understanding of these reactions in thylakoid membranes. Topics covered include the photosystems, the cytochrome b6-f complex, plastocyanin, ferredoxin, FNR, light-harvesting complexes, and the coupling factor. Chapters are also devoted to the structure of thylakoid membranes, their lipid composition, and their biogenesis. Updates on the crystal structures of cytochrome f, ATP synthase and photosystem I are presented and a section on molecular biology and evolution of the photosynthetic apparatus is also included. The chapters in this book provide a comprehensive overview of photosynthetic reactions in eukaryotic thylakoids. The book is intended for a wide audience, including graduate students and researchers active in this field, as well as those individuals who have interests in plant biochemistry and molecular biology or plant physiology.

Proteins

Author : David Whitford
Publisher : John Wiley & Sons
ISBN 13 : 1118685725
Total Pages : 544 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Proteins by : David Whitford

Download or read book Proteins written by David Whitford and published by John Wiley & Sons. This book was released on 2013-04-25 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins. An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.

Carotenoids

Author : John T. Landrum
Publisher : CRC Press
ISBN 13 : 1420052314
Total Pages : 562 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Carotenoids by : John T. Landrum

Download or read book Carotenoids written by John T. Landrum and published by CRC Press. This book was released on 2009-12-21 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carotenoids are of great interest due to their essential biological functions in both plants and animals. However, the properties and functions of carotenoids in natural systems are surprisingly complex. With an emphasis on the chemical aspects of these compounds, Carotenoids: Physical, Chemical, and Biological Functions and Properties presents a b

Sea Plants

Author :
Publisher : Elsevier
ISBN 13 : 012408107X
Total Pages : 593 pages
Book Rating : 4.1/5 (24 download)

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Book Synopsis Sea Plants by :

Download or read book Sea Plants written by and published by Elsevier. This book was released on 2014-05-21 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. The series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume, number 71, features reviews on sea plants. Its chapters cover topics such as the role of algae in sustainability; the status of kelp exploitation and marine agronomy; potential applications for enzymatic recovery of metabolites from seaweeds; and many more. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences Features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology Volume features reviews on sea plants

Regulation of Heat Shock Protein Responses

Author : Alexzander A A Asea
Publisher : Springer
ISBN 13 : 3319747150
Total Pages : 471 pages
Book Rating : 4.3/5 (197 download)

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Book Synopsis Regulation of Heat Shock Protein Responses by : Alexzander A A Asea

Download or read book Regulation of Heat Shock Protein Responses written by Alexzander A A Asea and published by Springer. This book was released on 2018-05-01 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: This books provides the most up-to-date reviews on current advances in our understanding of the regulation of heat shock protein responses. Key basic scientists and clinical research laboratories from major universities, academic medical centers and pharmaceutical companies around the world have contributed chapters that review present research activity and importantly project this field into the future. For easy readability, the book is sub divided into four sections, including, Section I - HSP and Stress Responses; Section II - Chaperone Functions of HSP; Section III - HSP in Human Diseases; Section IV - Prognosis & Diagnosis of HSP. The book is a must read for researchers involved in biomedical research, drug discovery and design to improve human health.

Metabolic Engineering

Author : Sang Yup Lee
Publisher : John Wiley & Sons
ISBN 13 : 352782345X
Total Pages : 1075 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Metabolic Engineering by : Sang Yup Lee

Download or read book Metabolic Engineering written by Sang Yup Lee and published by John Wiley & Sons. This book was released on 2021-06-02 with total page 1075 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Molecular Biology and Biotechnology of Plant Organelles

Author : Henry Daniell, Ph.D.
Publisher : Springer Science & Business Media
ISBN 13 : 1402031661
Total Pages : 671 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Molecular Biology and Biotechnology of Plant Organelles by : Henry Daniell, Ph.D.

Download or read book Molecular Biology and Biotechnology of Plant Organelles written by Henry Daniell, Ph.D. and published by Springer Science & Business Media. This book was released on 2007-11-04 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have taught plant molecular biology and biotechnology at the undergraduate and graduate level for over 20 years. In the past few decades, the field of plant organelle molecular biology and biotechnology has made immense strides. From the green revolution to golden rice, plant organelles have revolutionized agriculture. Given the exponential growth in research, the problem of finding appropriate textbooks for courses in plant biotechnology and molecular biology has become a major challenge. After years of handing out photocopies of various journal articles and reviews scattered through out the print and electronic media, a serendipitous meeting occurred at the 2002 IATPC World Congress held in Orlando, Florida. After my talk and evaluating several posters presented by investigators from my laboratory, Dr. Jacco Flipsen, Publishing Manager of Kluwer Publishers asked me whether I would consider editing a book on Plant Organelles. I accepted this challenge, after months of deliberations, primarily because I was unsuccessful in finding a text book in this area for many years. I signed the contract with Kluwer in March 2003 with a promise to deliver a camera-ready textbook on July 1, 2004. Given the short deadline and the complexity of the task, I quickly realized this task would need a co-editor. Dr. Christine Chase was the first scientist who came to my mind because of her expertise in plant mitochondria, and she readily agreed to work with me on this book.