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Protein Fractionation By Aqueous Two Phase Systems And Differential Ammonium Sulfate Precipitation
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Book Synopsis Protein Fractionation by Aqueous Two-phase Systems and Differential Ammonium Sulfate Precipitation by : Terasut Sookkumnerd
Download or read book Protein Fractionation by Aqueous Two-phase Systems and Differential Ammonium Sulfate Precipitation written by Terasut Sookkumnerd and published by . This book was released on 2000 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamental studies on aqueous two-phase systems and differential ammonium sulfate precipitation have been performed in this dissertation. The significant findings in this dissertation are: (1) A semi empirical correlation relating the partition coefficient of phase-forming polymers in aqueous two-phase systems to the differences in phase-forming polymer concentrations between the top and bottom phases has been developed. Good agreement between the correlation and the experimental results was obtained. (2) The partitioning behavior of PEG-protein conjugates in PEG/potassium phosphate and PEG/dextran systems has been investigated. A correlation that relates the partition coefficient of PEG protein conjugates to the partition coefficient of PEG and protein, the number of PEG conjugating to the protein, and the hydrophobicity changes due to a covalent bond and the protein's structural change is derived and tested with the experimental results. (3) Countercurrent distribution in aqueous two-phase systems and differential ammonium sulfate precipitation have been successfully applied to fractionate PEG-protein conjugates. (4) Regarding centrifugal precipitation chromatography, a mathematical model of ammonium sulfate gradient formation in the separation channel of centrifugal precipitation chromatography has been developed and solved numerically. To demonstrate the ability of the technique to purify proteins, mixtures of proteins were subjected to the technique. From the experiment, the resolution was a function of the degree of saturation of ammonium sulfate solution fed into the salt channel. (5) A mathematical model of protein retention in centrifugal precipitation chromatography has been developed and solved numerically. Using lysozyme as a protein model, the numerical results are compared with the experimental result and good agreements between the numerical and experimental results are achieved. (6) The effect of centrifugal precipitation chromatography on enzymatic activities of proteins has been investigated. Using alpha-chymotrypsin as a protein model, it is observed that the effect of fluid mixing in the separation channel of centrifugal precipitation chromatography on the enzymatic activity of alpha-chymotrypsin is minimal. Therefore, most proteins should remain active after being subjected to centrifugal precipitation chromatography. A better channel design and operating centrifugal precipitation chromatography at low temperature should reduce the losses in enzymatic activity of proteins further.
Book Synopsis Effects of Salts on the Phase Behavior of Proteins and Protein Mixtures by : Yu-Chia Cheng
Download or read book Effects of Salts on the Phase Behavior of Proteins and Protein Mixtures written by Yu-Chia Cheng and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Separating proteins in aqueous solutions through fractional precipitation by addition of salts is one of the oldest protein separation methods, and it remains the only purification step for some industrial enzymes and an initial purification step for higher-value therapeutic proteins. However, despite its widespread application, the fundamental characteristics of protein precipitation by salt are still incompletely understood. The primary focus of this thesis is the experimental exploration of the phase behavior of single and binary protein systems during protein precipitation in concentrated ammonium sulfate and sodium chloride solutions, and the effect of solution conditions on protein interactions. Protein precipitation phase behavior of single protein systems was measured as a function of solution conditions for lysozyme and ovalbumin. X-ray powder diffraction (XRD) and optical microscopy were used to characterize the structure of the protein precipitates. These studies showed that crystallization after initial precipitation is feasible even at high ionic strengths if sufficient mixing time is allowed, and such behavior may be a more general phenomenon although different crystallization pathways may be found in some systems and under different conditions; for instance, different pathways are seen in ovalbumin-ammonium sulfate systems as a function of pH. A more important development is the interpretation that emerges of precipitation as a manifestation of a metastable liquid-liquid separation, with the precipitate being a kinetically trapped dense liquid phase. Furthermore, the diversity of possible dense phases (amorphous precipitate, crystal phases or gel) associated with liquid-liquid phase separation shows that understanding the effects of protein interactions of different kinds and at different ranges could have important consequences for the systematic control of protein phase behavior. The phase behavior in selective precipitation of lysozyme-ovalbumin binary mixtures was investigated at various conditions. The best combination of selectivity and recovery of lysozyme was achieved in pH 7 NaCl solutions. At an initial concentration of 30 mg/g H 2 O of lysozyme and 30 mg/g H 2 O of ovalbumin, more than 80% of the lysozyme was collected in precipitate form with 93% purity at ionic strengths above 3 m, while ovalbumin was collected in the supernatant at 80% purity. The purities of both proteins were therefore improved by at least 30% in one step. Moreover, crystallization following initial precipitation was observed in some binary precipitation experiments and indicates that binary precipitation, like single protein precipitation, is a metastable equilibrium state. The observations of selective precipitation behavior were correlated with values of the osmotic second virial coefficient, measured by cross-interaction chromatography (CIC). The results show that interaction measures such as protein self- and cross-interactions can be used as a predictor for general trends and/or inconsistencies in the separation behavior and for optimizing working conditions. Such a mechanistic relationship can obviate the need for extensive trial-and-error methods in order to optimize conditions for protein separations.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2004 with total page 882 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Intensification of Biobased Processes by : Andrzej Gorak
Download or read book Intensification of Biobased Processes written by Andrzej Gorak and published by Royal Society of Chemistry. This book was released on 2018-06-18 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book dedicated entirely to this area, Intensification of Biobased Processes provides a comprehensive overview of modern process intensification technologies used in bioprocessing.
Book Synopsis Recombinant Protein Purification Using Thermoseparating Aqueous Two-phase Systems by : Josefine Persson
Download or read book Recombinant Protein Purification Using Thermoseparating Aqueous Two-phase Systems written by Josefine Persson and published by . This book was released on 1999 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Aqueous Two-Phase Systems by : Rajni Hatti-Kaul
Download or read book Aqueous Two-Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Book Synopsis Three Phase Partitioning by : Munishwar Nath Gupta
Download or read book Three Phase Partitioning written by Munishwar Nath Gupta and published by Elsevier. This book was released on 2021-08-11 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. Written by researchers who have been pioneers in developing and utilizing three phase partitioning Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols
Book Synopsis A Guide to Protein Isolation by : C. Dennison
Download or read book A Guide to Protein Isolation written by C. Dennison and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of "fundamentalness" appropriate to the task in hand. For example, an in-depth study of the mechanics of motor cars would tell one nothing about the dynamics of traffic. Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible.
Book Synopsis Guide to Protein Purification by : Murray P. Deutscher
Download or read book Guide to Protein Purification written by Murray P. Deutscher and published by Gulf Professional Publishing. This book was released on 1990 with total page 936 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
Book Synopsis Liquid Biphasic System by : Pau Loke Show
Download or read book Liquid Biphasic System written by Pau Loke Show and published by Elsevier. This book was released on 2020-11-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings. Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. Provides information and examples of advanced separations in a single source Includes detailed descriptions of novel bioseparation systems Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries
Book Synopsis Protein Purification by : Robert K. Scopes
Download or read book Protein Purification written by Robert K. Scopes and published by Springer Advanced Texts in Chemistry. This book was released on 1994 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide providing a brief account of the main protein fractionation methods, with some simple theoretical and thermodynamic explanations of the events occurring, for students and researchers involved in the process of isolating an enzyme, from whatever source. A basic background in biochemistry and protein chemistry is assumed.
Book Synopsis A Model for Phase Stability and Protein Partitioning in Aqueous Two-phase Systems by : Paul D. Grossman
Download or read book A Model for Phase Stability and Protein Partitioning in Aqueous Two-phase Systems written by Paul D. Grossman and published by . This book was released on 1987 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Ionic-Liquid-Based Aqueous Biphasic Systems by : Mara G. Freire
Download or read book Ionic-Liquid-Based Aqueous Biphasic Systems written by Mara G. Freire and published by Springer. This book was released on 2016-10-05 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.
Book Synopsis Protein Purification by : Michael R. Ladisch
Download or read book Protein Purification written by Michael R. Ladisch and published by . This book was released on 1990 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical volume gives real-life examples of how separation techniques are used in industry with recombinant proteins and emphasizes which techniques might be more useful than others. An introductory chapter ties together the various topics and associates the methods used with examples of large-scale methodologies. The emphasis of the volume is on how series can be applied to developing strategies and appropriate approaches for difficult protein separation problems. This valuable book provides an update on some of the existing technology used in industry and presents separation examples of several commercially important and recently developed proteins.
Book Synopsis Biocatalysis and biotransformation guided by protein engineering by : Hui-Min Qin
Download or read book Biocatalysis and biotransformation guided by protein engineering written by Hui-Min Qin and published by Frontiers Media SA. This book was released on 2023-03-23 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Separation of Protein Mixtures by Extraction by : Charles Allen Haynes
Download or read book Separation of Protein Mixtures by Extraction written by Charles Allen Haynes and published by . This book was released on 1992 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Basic Biotechniques for Bioprocess and Bioentrepreneurship by : Arvind Kumar Bhatt
Download or read book Basic Biotechniques for Bioprocess and Bioentrepreneurship written by Arvind Kumar Bhatt and published by Academic Press. This book was released on 2023-02-03 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Biotechniques for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from the basic laboratory techniques to scale-up, process development, demonstration, and finally its commercialization. The book compiles currently scattered materials on this topic and updates this information based on practical experience and requirements. The book will be an ideal source for new entrepreneurs who wish to start their own commercial units. Offers guidance for readers/researchers/start-ups/entrepreneurs on how to develop new microbiological and biotechnical processes Focuses on basic knowledge and possible solutions to the practical difficulties at all levels in one place through understanding of basic techniques in lab, during bioprocess development, commercialization, technology transfer, marketing, and others which is presently not available in the field Provides multifaceted coverage, with industry insights from experienced practitioners and leaders in the field Gives possible best solutions to the practical difficulties at all levels, i.e. lab, scaleup, and commercial stage Addresses ethical and other regulatory issues
