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Production Of Bioethanol From Bitter Cassava Optimization Of Simultaneous Saccharification Fermentation Process
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Book Synopsis Production of Bioethanol from Bitter Cassava: Optimization of Simultaneous Saccharification & Fermentation Process by : Muaz Mohd Zaini Makhtar
Download or read book Production of Bioethanol from Bitter Cassava: Optimization of Simultaneous Saccharification & Fermentation Process written by Muaz Mohd Zaini Makhtar and published by . This book was released on 2012 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bioethanol Production from Lignocellulosic Feedstock Using Aqueous Ammonia Pretreatment and Simultaneous Saccharification and Fermentation (SSF) by : Xuan Li
Download or read book Bioethanol Production from Lignocellulosic Feedstock Using Aqueous Ammonia Pretreatment and Simultaneous Saccharification and Fermentation (SSF) written by Xuan Li and published by . This book was released on 2010 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Simultaneous Liquefaction, Saccharification and Fermentation Process at Very High Gravity Using Cassava Flour for Ethanol Production and Valorization of Its By-product by Solid State Fermentation for Animal Feeds by : Dinh Vuong Mai
Download or read book Simultaneous Liquefaction, Saccharification and Fermentation Process at Very High Gravity Using Cassava Flour for Ethanol Production and Valorization of Its By-product by Solid State Fermentation for Animal Feeds written by Dinh Vuong Mai and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A simultaneous liquefaction, saccharification and fermentation (SLSF) process at very high gravity of cassava flour for potable ethanol production was developed at lab (1 L) and pilot scale (15 L and 1000 L). Cassava flour (CF) was mixed with tap water to obtain a concentration of 270 g/L dry matter. Sequentially, the mixture of a native starch hydrolyzing enzyme containing a mixture of alpha-amylase and gluco-amylase (Stargen 002 at 2565 GAU/kg CF), gluco-amylase (Amigase Mega L at 0.105% w/w), active dry yeast Saccharomyces cerevisiae (Red Ethanol at 3,5 × 10^7 cells/ml), KH2PO4 (9,9 mM) and urea (16.0 mM) was added into cassava slurry. The SLSF-VHG process including three steps of liquefaction, saccharification and fermentation was simultaneousely carried out in a same bioreactor, at 30°C. Under these conditions, the SLSF process finished after 144h fermentation with the ethanol concentration of 14,4% v/v corresponding to the theoretical ethanol yield of 86,0%. This SLSF-VHG process at very high gravity at pilot scale-1000 L was scaled up in order to evaluate the ethanol yield, energy consumption, production cost and environmental impact (greenhouse gas emissions). The results showed that the ethanol concentration of 13,9% v/v corresponding to a yield of 83,1% of the theoretical ethanol yield was obtained. The GHG emissions of cassava based SLSF process were 186,281 kg CO2eq. By using Response Surface Methodology, the quantity of Stargen 002; Amigase Mega L and yeast inoculation cells was reduced 13,1; 26,6 and 18,0%, respectively. Under these optimized conditions, for SLSF-VHG process at pilot scale 15L, the ethanol concentration obtained was 14,1% v/v corresponding to a yield of 84,0% of the theoretical ethanol yield.Cassava-based distillers dried grains (CDDG) obtained from SLSF-VHG process and from the ethanol plants using conventional process in Vietnam was determined the nutritional compositions. The results showed that, the CDDG produced from the SLSF-VHG and from conventional process had a high content of crude fiber (29,3 and 34,4%, respectively) and a low content of protein (13,2 and 11,9%, respectively). More value can be added to ethanol by-product through solid-state fermentation (SSF) using mold and yeast for animal feed. SSF were conducted with CDDG in 8 and 5 days of fermentation, respectively. Under optimal conditions, the crude protein fraction of CDDG fermented by Trichoderma harzianum BiomaTH1 or Yarrowia lipolytica W29 was increased from 11,84% DM for unfermented sample to 15,29 and 14,06% DM, respectively. In addition, the total amino acids of fermented samples using T. harzianum and Y. lipolytica was increased from 11,01 % DM to 13,86 % DM and 12,39 % DM along with an increase in the essential amino acids content which enhanced by 55% and 22%, respectively, including limiting amino acids in pig feeds. The in vitro protein digestibility was improved significantly from 82,5% to 89,2 and 86,9% for T. harzianum and Y. lipolytica fermentation, respectively.
Book Synopsis Lignocellulose Conversion by : Vincenza Faraco
Download or read book Lignocellulose Conversion written by Vincenza Faraco and published by Springer Science & Business Media. This book was released on 2013-06-12 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol has been recognized as a potential alternative to petroleum-derived transportation fuels. Even if cellulosic biomass is less expensive than corn and sugarcane, the higher costs for its conversion make the near-term price of cellulosic ethanol higher than that of corn ethanol and even more than that of sugarcane ethanol. Conventional process for bioethanol production from lignocellulose includes a chemical/physical pre-treatment of lignocellulose for lignin removal, mostly based on auto hydrolysis and acid hydrolysis, followed by saccharification of the free accessible cellulose portions of the biomass. The highest yields of fermentable sugars from cellulose portion are achieved by means of enzymatic hydrolysis, currently carried out using a mix of cellulases from the fungus Trichoderma reesei. Reduction of (hemi)cellulases production costs is strongly required to increase competitiveness of second generation bioethanol production. The final step is the fermentation of sugars obtained from saccharification, typically performed by the yeast Saccharomyces cerevisiae. The current process is optimized for 6-carbon sugars fermentation, since most of yeasts cannot ferment 5-carbon sugars. Thus, research is aimed at exploring new engineered yeasts abilities to co-ferment 5- and 6-carbon sugars. Among the main routes to advance cellulosic ethanol, consolidate bio-processing, namely direct conversion of biomass into ethanol by a genetically modified microbes, holds tremendous potential to reduce ethanol production costs. Finally, the use of all the components of lignocellulose to produce a large spectra of biobased products is another challenge for further improving competitiveness of second generation bioethanol production, developing a biorefinery.
Book Synopsis Bio-Ethanol from sweet sorghum by : Sirinuch Chindaruska
Download or read book Bio-Ethanol from sweet sorghum written by Sirinuch Chindaruska and published by Lampi di stampa. This book was released on 2008-09-01 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the recent years there is a growing worldwide interest in the use of biofuels as they can reduce the CO 2 emissions of fuels used in transportation, diversify energy procurement, contribute to a longterm replacement for oil and offer new earning opportunuties for the rural sector. This book by dr. Sirinuch Chindaruksa and dr. Michela Pin represents a humble, but effective and timely, contribution to assessing the role that biofuels can play. It is devoted to a careful discussion of sweet sorghum as a suitable (profitable, promising) raw material for bioethanol production, including the analysis of a small scale plant. Sirinuch Chindaruksa e Michela Pin in modo semplice, danno un contributo all’attualissimo e diffuso argomento dell’uso di biocarburanti nei mezzi di trasporto al fine di ridurre l’emissione di CO2. Nel libro è esposta un’argomentazione accurata sul sorgo zuccherino come materia prima idonea ed economicamente vantaggiosa nella produzione di bioetanolo.
Book Synopsis Bioethanol Production from Office Waste Using Saccharomyces Cerevisiae Via Simultaneous Saccharification Fermentation (SSF) by :
Download or read book Bioethanol Production from Office Waste Using Saccharomyces Cerevisiae Via Simultaneous Saccharification Fermentation (SSF) written by and published by . This book was released on 2012 with total page 61 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Enzyme Hydrolysis of Cassava Peels for Ethanol Production by : Ikuba John Ona
Download or read book Enzyme Hydrolysis of Cassava Peels for Ethanol Production written by Ikuba John Ona and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The enzyme hydrolysis of cassava peels for ethanol production provides an interesting research opportunity to convert starch rich lignocellulose waste into renewable fuel production. The research involved the pretreatment of cassava peels with steam explosion and hot water pretreatment processes as well as combining both amylolytic and cellulolytic enzymes to produce simple sugars. This research compared different enzyme treatment strategies; a separate hydrolysis that involved the treatment of the peels with either cellulolytic enzymes or amylolytic enzymes, a consecutive hydrolysis process which is a follow up of the separate hydrolysis in which sugars were washed from the initial enzyme treatment (amylase or cellulase treatment) and the cassava peels resuspended for further enzyme treatment was also investigated. Another treatment strategy employed in this study was the simultaneous hydrolysis by amylases and cellulases of the cassava peels. The hydrolysis rate and yield were compared for each process. Minor changes that incorporated steam explosion pretreatment and hot water pretreatment were also studied. A separate hydrolysis of milled cassava peels treated by amylolytic and cellulolytic enzymes yielded a maximum reducing sugar of 0.41g (as glucose) per gram of peels and 0.31g per gram of peels respectively. Also steam exploded cassava peels treated by amylolytic and cellulolytic enzymes yielded maximum reducing sugars of 0.24g per gram of peels and 0.37g per gram of peels respectively. Results also showed that a consecutive treatment that incorporates an initial hydrolysis by cellulolytic enzymes followed by a subsequent treatment by amylolytic treatment yielded reducing sugars of 0.64g per gram of milled cassava peels. A reverse treatment where the cellulolytic enzymes were used to first treat the peels before a second treatment by amylolytic enzymes yielded 0.61g reducing sugar per gram of milled cassava peels. A simultaneous hydrolysis by both cellulolytic and amylolytic enzymes produced a maximum reducing sugar of 0.58g per gram of milled cassava peels. A modification that incorporates hot water pretreatment, simultaneous and consecutive treatment was carried out. The milled cassava peels treated with hot water at 1000C and amylase enzymes for 2 hours were further subjected to a simultaneous saccharification by cellulases and glucoamylase enzymes yielded a reducing sugar of 0.62g per gram of peels. Fermentation experiments were also carried out with Kluyveromyces marxianus at 400C and results showed a maximum ethanol yield of 0.12g ethanol per g of cassava peels for a separate hydrolysis and fermentation process and 0.18g ethanol per g of cassava peels for the simultaneous saccharification and fermentation process. It was concluded that cassava peels presents a very good source of sugars for bioethanol production.
Book Synopsis Simultaneous Saccharification and Fermentation and Factors Influencing Ethanol Production in SSF Process by : Manikandan Kanagasabai
Download or read book Simultaneous Saccharification and Fermentation and Factors Influencing Ethanol Production in SSF Process written by Manikandan Kanagasabai and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ethanol production from agricultural products mainly corn, wheat, sweat potato and residue are gaining importance and requires an industrially viable novel technology namely simultaneous saccharification and fermentation process. This process has an advantage of carrying out saccharification using enzyme and fermentation using yeast in a single fermenter. The investment cost for industrial ethanol production using cheap agricultural residues can be well achieved using SSF process. The success of SSF process greatly depends upon the pretreatment methods using different enzymes to break the complex carbohydrates to simple sugars. Optimization of key process variables is essential to maximize the ethanol yield from suitable substrates. The key process variables affecting the SSF process are pH, temperature, fermentation time, enzyme concentration and substrate concentration. The medium components are to be screened for effective nitrogen, potassium and phosphorous sources to increase the ethanol yield.
Book Synopsis Second-Generation Bioethanol Production Through a Simultaneous Saccharification-Fermentation Process Using Kluyveromyces Marxianus Thermotolerant Yeast by : Jorge A. Mejía-Barajas
Download or read book Second-Generation Bioethanol Production Through a Simultaneous Saccharification-Fermentation Process Using Kluyveromyces Marxianus Thermotolerant Yeast written by Jorge A. Mejía-Barajas and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the present renewable fuels demand increase, reduction of second-generation bioethanol production cost is pursued, since it is considered the most promising biofuel, but not yet economically viable. A proposed solution is its production through a simultaneous saccharification and fermentation process (SSF); however, it is necessary to apply temperatures above 40°C, which reduce the viability of traditional ethanologenic yeasts. As consequence, the use of thermotolerant ethanologenic yeast has been suggested, among which the yeast Kluyveromyces marxianus stands out. This chapter addresses the production of second-generation bioethanol through the SSF process, emphasizing the potential of K. marxianus to transform lignocellulosic biomass as agave bagasse. As result, it is proposed to direct the second-generation bioethanol production to the SSF process employing thermotolerant yeasts, to increase process productivity, and addressing the economic barriers.
Book Synopsis Bioethanol Production from Cassava by Fermentation Process Using Saccharomyces Cerevisiae by : Kanagaraj Rajandran
Download or read book Bioethanol Production from Cassava by Fermentation Process Using Saccharomyces Cerevisiae written by Kanagaraj Rajandran and published by . This book was released on 2013 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol can be produced by fermentation of cassava, which carried out in conical flask by using Saccharomyces Cerevisiae. Objective of this experiment is to find out the effect of agitation speed, temperature and substrate concentrations on the bioethanol production, cell growth and the glucose consumption. At the end of this experiment, the best substrate concentrations of cassava, which leads to highest production of bioethanol and the highest cell growth can be identified. In this study substrate concentration varied as 25g (6.25%w/v), 50g (12.5% w/v), 75g (18.75% w/v), 100g (25% w/v) and 150g (32.5%w/v). Follows that, the effect of various fermentation temperature will be analyze, by using temperature varies as 20oC, 30OC, 40OC and 50oC. From this temperature vary the growth of yeast and the bioethanol production will be analyzed. Follows that, third parameter of this experiment is agitation speed of fermentation shake flask. Agitation speed varies from 0 rpm, 150rpm, 200 rpm and 250 rpm. For this part, effect of agitation speed on bioethanol production and cell growth of yeast studied. As for the optimum condition, temperature 30oC maintained, agitation speed fixed at 200 rpm, pH maintained at 4.5 and substrate concentration fixed about 100g (25% w/v). This experiment begins with inoculum preparation step, medium preparation step, following by transferring the inoculum into medium into 1L conical flask, fermentation of cassava in incubator shaker and lastly end with sample analysis. From the experiment, agitation speed 200 rpm, substrate concentration of 150g and temperature 40OC gives highest bioethanol production, highest cell growth and highest glucose consumption.
Book Synopsis Intensification of Bioethanol Production by Simultaneous Saccharification and Fermentation in an Oscillatory Baffled Reactor by : Joseph Ikwebe
Download or read book Intensification of Bioethanol Production by Simultaneous Saccharification and Fermentation in an Oscillatory Baffled Reactor written by Joseph Ikwebe and published by . This book was released on 2013 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Process Design of Ethanol Production System from a Semi-solid Mixture of Raw Cassava Starch. Rhizopus Sp. and S.Cerevisiae by Simultaneous Saccharification, Fermentation and Stripping by : F. B. Elegado
Download or read book Process Design of Ethanol Production System from a Semi-solid Mixture of Raw Cassava Starch. Rhizopus Sp. and S.Cerevisiae by Simultaneous Saccharification, Fermentation and Stripping written by F. B. Elegado and published by . This book was released on 1988 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: The general objective of developing a laboratory scale process of ethanol production system from raw cassava starch has been attained. The specific objectives have likewise been satisfied such as the screening of the best fungal microorganism for raw starch saccharification, optimazation of air-agitated saccharification of semi-solid raw cassava starch and the designing of a laboratory scale bioreactor, column and other devices. The bench sale data obtained showed that the simultaneous saccharification and fermentation process coupled with ethanol stripping and rectifying system has been found to be feasible at laboratory scale and was reasonably efficient based on the starch to ethanol conversion, concentration of the ethanol in the final product and ethanol productivity. Further studies are seen as necessary in order to thoroughly assess the applicability of the process either at village-scale technology or for industrial applications.
Book Synopsis Production of Ethanol from Apple Pomace Using Saccharomyces Cerevisiae, Trichoderma Reesei and Myceliophthora Thermophila Fungi by : Anantveer Kailay
Download or read book Production of Ethanol from Apple Pomace Using Saccharomyces Cerevisiae, Trichoderma Reesei and Myceliophthora Thermophila Fungi written by Anantveer Kailay and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "In recent years, fuels from renewable sources have been gaining importance as an alternative to conventional fuels to reduce greenhouse gases and avail long term sustainability. Biofuels from fruit pomaces are gaining attention due to their richness in sugar content and their accessibility and low cost for the production of biofuels. The aim of this study is to provide a viable solution to the environmental problems caused by apple pomace waste through the optimization of bioethanol production from this available biomass. Two fungal strains, Trichoderma reesei, and Myceliophthora thermophila were used for saccharification of apple pomace and the sugars obtained from the saccharification process were fermented using the fungus Saccharomyces cerevisiae for the production of ethanol. The optimization steps for efficient saccharification were performed in order to study the significant factors such as temperature, moisture level, incubation time and the effect of supplementation of apple pomace with peptone as a nitrogen source. According to the results of the optimization process for saccharification, the highest values of sugar content were observed at a moisture content (wet basis) of 90% for M. thermophila and 85% for T. reesei. Based on the statistical analysis, the effect of temperature was not significant on sugar yield however the maximum yield was obtained at 28°C. 1% w/w peptone was found to be the most effective dose to increase the sugar yield for both the fungal strains tested for saccharification. The apple pomace treated with T. reesei and M. thermophila yielded 6.11 % (v/w) and 3.96% (v/w) amount of ethanol, respectively, after fermentation using S. cerevisiae. However, only 3.72% alcohol was obtained from the apple pomace treated with only S. cerevisiae as a control. Furthermore, the results revealed that using T. reesei with S. cerevisiae is an effective way for the production of bioethanol from apple pomace. Fermentation of apple sludge using S. cerevisiae was also carried out. Fermentation of apple filtered sludge resulted in 6.54% (v/w) amount of ethanol using S. cerevisiae and fermentation of unfiltered sludge with similar conditions resulted in 8.05% (v/w) of ethanol at 72 hours." --
Book Synopsis Bioethanol: A Green Energy Substitute for Fossil Fuels by : Eriola Betiku
Download or read book Bioethanol: A Green Energy Substitute for Fossil Fuels written by Eriola Betiku and published by Springer Nature. This book was released on 2023-08-28 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book looks deeply into the prospects for using ethanol as a greener alternative to fossil fuels and the technical and scientific issues that surround them. Ethanol, with its numerous advantages, has emerged as a promising contender to replace gasoline as a fuel source. Currently, it is commercially available as a blend with gasoline, commonly known as E10 and E25, utilizing various ratios of ethanol. Despite its clear benefits over gasoline, the widespread adoption of ethanol as a fuel remains hindered by its limited availability. In this insightful book, we aim to explore the multifaceted challenges surrounding ethanol's full integration into our energy landscape, employing a comprehensive approach through review manuscripts. Leading worldwide experts, known for their deep understanding of ethanol as a fuel, have contributed to the book. Their valuable insights and contributions enrich the book's content, offering readers a comprehensive exploration of the subject matter. This book is a compelling resource for researchers, energy professionals, and anyone interested in understanding the challenges and opportunities associated with the integration of ethanol as a substitute for gasoline.
Book Synopsis Current Developments in Biotechnology and Bioengineering by : Mohammad Taherzadeh
Download or read book Current Developments in Biotechnology and Bioengineering written by Mohammad Taherzadeh and published by Elsevier. This book was released on 2022-11-18 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: Filamentous Fungi Biorefinery, the latest release in the Current Developments in Biotechnology and Bioengineering series, builds on knowledge on the classification of filamentous fungi and presence and roles played in ecosystems. The importance of filamentous fungi is then further corroborated through a description of their present applications in biotechnological processes. Knowledge on fungal biology is extended through discussion on structure and composition together with a description of growth potentialities of filamentous fungi in/on a wide range of substrates. In addition, the morphology of filamentous fungi is then described and its implications during integration in industrial processes is discussed. The book then provides an overview on the use of filamentous fungi for the production of a wide range of value-added products, including feed and food products, alcohols, organic acids, pigments, enzymes, antibiotics and biopolymers. All provided state-of-arts are extended to a description of the present degree of application of filamentous fungi towards the production of those products using low-value substrates, identification of research gaps, and proposes future research avenues. - Presents the first book dedicated to the use of filamentous fungi for process development within waste management - Discusses the transfer of research knowledge into industrial processes and marketable products - Includes industrial applications of filamentous fungi towards valorization of low-value substrates - Provides up-to-date knowledge on research and application fields that can benefit from the integration of filamentous fungi
Book Synopsis Bioethanol Production from Food Crops by : Ramesh C. Ray
Download or read book Bioethanol Production from Food Crops written by Ramesh C. Ray and published by Academic Press. This book was released on 2018-08-20 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol Production from Food Crops: Sustainable Sources, Interventions and Challenges comprehensively covers the global scenario of ethanol production from both food and non-food crops and other sources. The book guides readers through the balancing of the debate on food vs. fuel, giving important insights into resource management and the environmental and economic impact of this balance between demands. Sections cover Global Bioethanol from Food Crops and Forest Resource, Bioethanol from Bagasse and Lignocellulosic wastes, Bioethanol from algae, and Economics and Challenges, presenting a multidisciplinary approach to this complex topic. As biofuels continue to grow as a vital alternative energy source, it is imperative that the proper balance is reached between resource protection and human survival. This book provides important insights into achieving that balance. - Presents technological interventions in ethanol production, from plant biomass, to food crops - Addresses food security issues arising from bioethanol production - Identifies development bottlenecks and areas where collaborative efforts can help develop more cost-effective technology
Book Synopsis Handbook on Bioethanol by : Charles Wyman
Download or read book Handbook on Bioethanol written by Charles Wyman and published by Routledge. This book was released on 2018-05-02 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol is a versatile transportation fuel and fuel additive that offers excellent performance and reduced air pollution compared to conventional fuels. Its production and use adds little, if any, net release of carbon dioxide to the atmosphere, dramatically reducing the potential for global climate change. Through a sustained research program and an emerging economic competitiveness, the technology for bioethanol production is poised for immediate widespread commercial applications. Written by engineers and scientists providing a technical focus, this handbook provides the up-to-date information needed by managers, engineers, and scientists to evaluate the technology, market, and economics of this fuel, while examining the development of production required to support its commercial use.
