Production of Abe (acetone-butanol-ethanol) from Pome by Clostridium Beijerinckii

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Total Pages : 66 pages
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Book Synopsis Production of Abe (acetone-butanol-ethanol) from Pome by Clostridium Beijerinckii by : Nur Akmalina Azeman

Download or read book Production of Abe (acetone-butanol-ethanol) from Pome by Clostridium Beijerinckii written by Nur Akmalina Azeman and published by . This book was released on 2013 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaysia was one of the biggest producers and exporters of palm oil and palm oil products (Malaysian Palm Oil Council, 2012). Thus, the total oil palm cover has increased with a corresponding increase in palm oil production. As a result, palm oil waste which was a by-product of the milling process will also increase. Due to the presence of high total solids in palm oil mill effluent (POME), attempts have been made to convert this waste into valuable products such as feed stock (Rupani et al., 2010). Consequently, purpose of this research was to study about the production of acetone-butanol-ethanol (ABE) solvents using POME by Clostridia beijerinckii. These ABE solvents which are consist of acetone, butanol and ethanol have each one benefits such as butanol used for biofuel, acetone used for making plastic and ethanol can be used as disinfectant. The culture was grown in RCA and then 10% v/v of the culture was transferred to RCM for preparation of inoculum medium. After that, 10% v/v of inoculum medium was transferred into fermentation medium which consists of 90% of POME. In this research, POME would be used as substrate medium. Then, the fermentation medium was run to find the optimum pH for fermentation medium (initial pH 5.4 until 6.2), concentration of POME (80% to 100% v/v) and temperature of fermentation (27°C to 47°C). The highest value of total ABE obtained was 0.771 g/L with 90% concentration at condition of pH 5.8 of 37°C.

Solvent Production from Palm Oil Mill Effluent (POME) by Clostridium Beijerinckii

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Total Pages : 54 pages
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Book Synopsis Solvent Production from Palm Oil Mill Effluent (POME) by Clostridium Beijerinckii by : Ma Umaira Suhaddha Zainal Abidin

Download or read book Solvent Production from Palm Oil Mill Effluent (POME) by Clostridium Beijerinckii written by Ma Umaira Suhaddha Zainal Abidin and published by . This book was released on 2012 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: Acetone, butanol and ethanol have widely been used in many industries. butanol and ethanol have been recognize as potential biofuel. While acetone is important as fuel tracer in field of combustion and use as suspension agent in pharmaceutical preparation. In 1990s, the increasing price of sugarcane have increased the production cost of ABE fermentation and the only factor that causing ABE fermentation could not survive was due to the cost of raw materials of substrate By using POME as substrate for fermentation process of acetone, butanol and ethanol (ABE), production is at advantage due to its lower cost and readily available. This study is to determine the effect of concentration of substrate, modified glucose concentration on substrate and initial pH of substrate towards enhancing solvent production from palm oil mill effluent (POME) by Clostridium beijerinckii. The experiment has been carried out in a batch culture system. The study of substrate pH effect ranged from 4.7, 5.2, 5.7 to 6.2. Meanwhile, for study of substrate concentration ranged from 60%, 70%, 80%, 90% to 100%. The study of modified substrate concentration effect were ranging from 20g/L, 30g/L, 40g/L, 50g/L to 60g/L. The fermentation of POME which produced acetone, butanol and ethanol, was analysed using gas chromatography flame ionization detector (GC-FID) and isobutanol was used as the internal standard. The experiment was using one factor at one time (OFAT) method to determine the best range values. From the parameters stated, sedimented POME at 70% concentration with initial pH increased to 5.7 is most suitable for solvent production 70% with 1.025g/L solvent produced. While 50g/L is the optimal concentration of modified glucose that enhanced the solvent production with supported by operating temperature at 37°c and 150rpm which 1.241g/L solvent produced. Apart from that, growth cell at pH5.7 maximize the solvent production where 0.967g/L solvent produced. In addition, DNS test for glucose consumption of all parameters studies can be conclude that higher glucose consumption resulted in higher amount of total solvent produced. The results obtained from these experiments showed that POME could be a viable media for ABE fermentation.

Effect of Substrate Concentration and Agitation Rate on Butanol Production from POME by Clostridium Beijerinckii

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ISBN 13 :
Total Pages : 63 pages
Book Rating : 4.:/5 (953 download)

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Book Synopsis Effect of Substrate Concentration and Agitation Rate on Butanol Production from POME by Clostridium Beijerinckii by : Ellie Fazlin Mohd Sarian

Download or read book Effect of Substrate Concentration and Agitation Rate on Butanol Production from POME by Clostridium Beijerinckii written by Ellie Fazlin Mohd Sarian and published by . This book was released on 2010 with total page 63 pages. Available in PDF, EPUB and Kindle. Book excerpt: Palm oil mill effluent (POME) is the largest by product generated from palm oil mills, about 50 million tonnes annually. POME has a potential substrate for acetone, butanol and ethanol production using Clostridium beijerinckii. This research mainly studied on the direct use of POME as fermentation medium for solvent fermentation by Clostridium beijerinckii ATCC 51743. This research also investigated the growth profile rate and the consumption of glucose by Clostridium beijerinckii during fermentation. Fermentation were conducted for 72 hours at 370C by using palm oil mill effluent and reinforced clostridia medium as a growth medium in batch culture. Investigations were carried out on the effect of subsrate (POME) concentration and the agitation rate into the production of butanol. The POME concentration that had been used in this investigation were 90%, 80% and 70% while the agitation rate that had been used were 0 rpm, 100 rpm, 175 rpm and 250 rpm respectively. The results showed that the highest yield of butanol produced was 3.12 g/L by 70% POME at 100 rpm of agitation rate.

Acetone-butanol-ethanol Fermentation by Engineered Clostridium Beijerinckii and Clostridium Tyrobutyricum

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Total Pages : pages
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Book Synopsis Acetone-butanol-ethanol Fermentation by Engineered Clostridium Beijerinckii and Clostridium Tyrobutyricum by : Wei-Lun Chang

Download or read book Acetone-butanol-ethanol Fermentation by Engineered Clostridium Beijerinckii and Clostridium Tyrobutyricum written by Wei-Lun Chang and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A pilot scale FBB was constructed for the evaluation of the feasibility of large scale butanol production in the FBB. Two batch fermentations were operated and the results of ABE fermentation were similar to those at lab scale. ABE fermentation at pilot scale was able to produce butanol at ~9.1 g/L with media containing glucose and butyric acid.

Effect of Fermentation Time on Butanol and Ethanol Production from Palm Oil Mill Effluent by Clostridium Acetobutylicum

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ISBN 13 :
Total Pages : 75 pages
Book Rating : 4.:/5 (953 download)

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Book Synopsis Effect of Fermentation Time on Butanol and Ethanol Production from Palm Oil Mill Effluent by Clostridium Acetobutylicum by : Muhammad Zaini Othman

Download or read book Effect of Fermentation Time on Butanol and Ethanol Production from Palm Oil Mill Effluent by Clostridium Acetobutylicum written by Muhammad Zaini Othman and published by . This book was released on 2010 with total page 75 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofuels production through fermentation from renewable waste has been put more to focus in mid 20th century till now. Main focus of the research is to determine optimum fermentation time to obtain maximum concentration of butanol and ethanol via ABE fermentation. The ABE fermentation is a two phase chemical production which is from acidogenesis into solventogenesis. The fermentation is caused by clostridia bacteria and in this case of study is Clostridium acetobutylicum pattered NCIMB 13357. The main substrate for nutrient in this study is Palm Oil Mill Effluent (POME) which freshly taken from Lepar Hilir, Pahang. Through the research, POME was found to contain majorly of pentose and carbohydrate substance such as galactose, glucose, fructose, sucrose and lactose. The research parameter is fermentation time at 72, 80, 48 and 60 hours. The experiments start from medium preparation, cultivation of bacteria, inoculation, fermentation and lastly analysis of product. All standard solution is made in order to standardize the reading of concentration of product and substance analyzed. The condition for bacteria environment is set to anaerobic and optimum pH at 5.8, temperature at 37 0C and agitation speed is set at 200 rpm along ABE fermentation. Final result shown that at fermentation time of 40 hours, the maximum value of 0.07 g/L of butanol was produced, while for ethanol is 83.499 g/L at 20 hour fermentation time. POME is encouragingly valid for use as fermentation media as it contain many utilizable substrates for Clostridium acetobutylicum.

Engineering Microbial Fermentations

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ISBN 13 :
Total Pages : 130 pages
Book Rating : 4.:/5 (957 download)

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Book Synopsis Engineering Microbial Fermentations by : Zachary Cardwell Baer

Download or read book Engineering Microbial Fermentations written by Zachary Cardwell Baer and published by . This book was released on 2014 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: The batch fermentation of simple sugars to ethanol has thus far been the most successful process to displace transportation fuel consumption worldwide. However, the physical properities of ethanol limit its application in most of the world beyond 10-15 vol% blends with conventional gasoline. Additionally, ethanol is too volatile to be blended at an appreciable level with jet and diesel fuels. Alternative technologies are necessary to produce compounds that can be blended in all forms of transportation fuel and to higher levels. The biological production of longer chain oxygenates or hydrocarbons has been purposed to address this challenge. However, the increased toxicity of these molecules has limited their usefulness to-date. Alternatively, chemocatalytic routes to produce these fuel molecules do not suffer from toxicity issues but the highly functional saccharide-based feedstocks result in undesirable by-product formation and yield losses. We propose that by effectively combining these biological and chemocatalytic approaches efficient production of all classes of transportation fuels can be achieved. Clostridium acetobutylicum is a well-studied industrial bacterium capable of fermenting a wide-variety of saccharides into a mixture of acetone, butanol, and ethanol (ABE). These mixed products contain functionalities that are amenable to C-C bond formation through transition-metal catalysis. Thus enabling the production of a suite of long-chain oxygenates from a variety of sugar substrates. This dissertation describes three major topics associated with the integration biological and chemocatalytic processes for fuel production: (1) the initial discovery and evaluation of such a combined process, (2) tailoring the fermentation products and catalyst to favor diesel production, and (3) engineering tunable ethanol and acetone co-production in Escherichia coli. Additionally, a thorough and reproducible protocol describing both biological and chemocatalytic procedures are provided. Acetone, a product of the acetone-butanol-ethanol (ABE) fermentation, harbors a nucleophilic [alpha]-carbon, which is amenable to C-C bond formation with the electrophilic alcohols (ethanol and n-butanol) produced in ABE fermentation. This functionality enables the formation of higher molecular weight hydrocarbons similar to those found in current gasoline, jet, and diesel fuels. Using a palladium-catalyzed alkylation reaction efficient conversion of ABE fermentation products into ketones, ranging from 2-pentanone to 6-undecanone was achieved. Tuning of the reaction conditions permits production of either predominately gasoline or jet and diesel precursors. Glyceryl tributyrate was identified as a suitable extractant for selective in situ removal of both acetone and alcohols. Enabling simple integration of ABE fermentation and water sensitive chemical catalysis, while reducing the energy demand of the overall process. Additionally, extractive fermentation with glyceryl tributyrate removed several lignocellulosic-derived pretreatment inhibitors completely restoring solvent production titers. Tailoring both the biological fermentation and chemocatalytic reaction conditions predictably increased the production of sustainable diesel blendstocks from lignocellulosic sugar. More specifically, engineering the metabolism of C. acetobutylicum to produce isopropanol-butanol-ethanol (IBE) as opposed to ABE by expression of the secondary alcohol dehydrogenase (sadh) from C. beijerinckii strain B593. Coupled with the optimization of a more water-resistant alkylation catalyst, hydrotalcite-supported copper(II) or palladium (0). Furthermore, in silco predictions of extraction efficiency using COSMO-RS were employed to identify oleyl alcohol as a superior extractant for in situ removal of IBE. To achieve highly controllable acetone and ethanol production both metabolic and fermentation engineering approaches were employed. Strains capable of producing acetone without carboxylic acid reassimilation through a newly described pathway were developed. Furthermore, a more oxidized sugar-acid (gluconic acid) was used to drive production of acetone through redox balancing of NADH, predicatively reducing the molar ethanol:acetone ratio. Increases in the molar ethanol:acetone ratio were also described by the co-expression of either the aldehyde/alcohol dehydrogenase (adhE) from E. coli MG1655 or pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) from Z. mobilis. Acetone titers reached wild-type C. acetobutylicum levels by controlling the fermentation sparge rate and pH in a bioreactor. Finally, catalytic strategies to upgrade the co-produced ethanol and acetone at both low and high molar ratios are described. By leveraging the advantages of both biological fermentation and chemocatalytic reactions of fermentation products with well-defined functionality we are able to efficiently convert a variety of saccharides into long-chain oxygenates. These long-chain oxygenates can be blended with all forms of transportation fuels. Additionally, tuning the microorganism's metabolism, fermentation conditions, catalysis reaction, or all of the above can optimize the production for a specific class of fuel. The integration of mixed-product fermentation with chemical catalysis is thus a novel and potentially enabling route for the economical conversion of biomass into liquid transportation fuels.

Fermentative Production of Butanol from Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii

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ISBN 13 :
Total Pages : 57 pages
Book Rating : 4.:/5 (953 download)

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Book Synopsis Fermentative Production of Butanol from Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii by : Farhanah Amanulla Khan

Download or read book Fermentative Production of Butanol from Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii written by Farhanah Amanulla Khan and published by . This book was released on 2013 with total page 57 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaysia is the one of world's largest producer of palm oil. Waste from palm oil production factories has been increasing rapidly and one of the serious problems in the palm oil fruit processing is managing the waste generated by the processes. Recent studies showed that butanol can be used as excellent renewable liquid transportation fuel. In this study, oil palm trunk sap is used as the substrate. The objective of this study is to compare the production of biobutanol from biomass waste (oil palm trunk sap) via fermentation process using different microorganisms. The fermentation type done was submerged fermentation. Fermentation was conducted for five days at 37 0C. Samples were taken for every 6 hours for 3 days, then for every 12 hours. Samples taken were analyzed using gas chromatography-flame ionization detector (GC-FID) and high performance liquid chromatography (HPLC) was used to analyze sugar composition in OPT sap. UV Spectrophotometer is used to analyze biomass. Oil palm trunk (OPT) sap contains sucrose as its dominant sugar followed by glucose, fructose and galactose. Based on the analysis, acetone, butanol and ethanol were produced. Comparisons between the two strains Clostridium acetobutylicum and Clostridium beijerinckii were made, and C. beijerinckii showed higher production of acetone, butanol and ethanol. The highest yield for butanol production by C. acetobutylicum and C. beijerinckii were 13.910 g/L and 15.301 g/L and highest production of ethanol were 11.17 g/L and 12.09 g/L. 4.13 g/L and 4.49 g/L acetone was produced by C. acetobutylicum and C. beijerinckii. Based on this study, it is concluded that OPT sap is preferable substrate to produce butanol by using C. beijerinckii.

Butanol Production from Lignocellulosic Feedstocks by Acetone-butanol-ethanol Fermentation with Integrated Product Recovery

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (747 download)

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Book Synopsis Butanol Production from Lignocellulosic Feedstocks by Acetone-butanol-ethanol Fermentation with Integrated Product Recovery by : Congcong Lu

Download or read book Butanol Production from Lignocellulosic Feedstocks by Acetone-butanol-ethanol Fermentation with Integrated Product Recovery written by Congcong Lu and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: n-Butanol has been attracting research attention as a liquid biofuel recently, in addition to its current application as an industrial chemical and solvent. With the concerns of diminishing fossil reserves, environmental issues caused by greenhouse gas emission, and unstable supply and price spike of crude oil, renewed interest has returned to pursue biobutanol production through acetone-butanol-ethanol (ABE) fermentation as opposed to petrochemically-derived butanol. However, the conventional ABE fermentation suffers from many limitations, including low butanol titer, high cost of traditional food-based raw materials, end-product inhibition and high butanol recovery cost by distillation, which negatively impacts the process efficiency and economics. Fortunately, these hurdles are being overcome by technological advances on ABE fermentation in the past few decades. Research on genetic modifications and chemical mutation of solventogenic Clostridia has focused on obtaining mutant strains with enhanced butanol producing ability. Adequate research success in utilizing renewable and sustainable lignocellulosic biomass has identified a novel group of cost-effective feedstocks for ABE fermentation in replacement of the traditional costly starch and sugar-based substrates. Novel fed-batch and continuous fermentation processes with cell immobilization and cell recycle have been developed for more efficient substrate conversion and butanol production. When further integrated with alternative energy-efficient butanol recovery techniques, such as gas stripping and pervaporation, the integrated ABE fermentation process can achieve high overall butanol production, reactor productivity, sugar conversion, and simplified downstream separation. Therefore, the overall goal of this project was to develop a process to produce butanol through ABE fermentation by hyper-butanol-producing mutants using lignocellulosic biomass, and integrate online product recovery to achieve enhanced overall butanol production and process efficiency. Corn fiber, cassava bagasse, wood pulp and sugarcane bagasse were investigated as potential feedstocks for butanol production from ABE fermentation, and gas stripping as the online butanol recovery technique was evaluated and integrated with ABE fermentation. In batch fermentations, immobilized mutant strain C. beijerinckii JB 200 produced 12.7 g/L and 15.4 g/L ABE from corn fiber hydrolysate and cassava bagasse hydrolysate in a fibrous bed bioreactor, respectively. C. beijerinckii CC101 produced 11.35 g/L ABE from wood pulp hydrolysate, and its recombinant mutant CC101-SV6 produced 9.44 g/L ABE from sugarcane bagasse hydrolysate in free-cell batch fermentations. ABE production from wood pulp hydrolysate was further enhanced to 17.73 g/L in a gas stripping integrated ABE batch fermentation process, with a higher ABE yield of 0.44 g/g compared with 0.39 g/g from non-integrated batch process. Concentrated cassava bagasse hydrolysate containing 584.4 g/L glucose was utilized by C. beijerinckii JB 200 in an integrated fed-batch ABE fermentation process, and 90.3 g/L ABE were produced with a productivity of 0.53 g/L. h, which was further improved to 108.5 g/L with nutrient supplementation. This project demonstrated that butanol can be produced from various lignocellulosic feedstocks, from agricultural biowastes to woody biomass residues. By employing mutant strains of solventogenic Clostridia bacteria, different fermentation modes, and online product recovery, an integrated process was developed for the production of n-butanol that can potentially replace petroleum-based butanol.

Effect of Time and Temperature on Butanol Production from Palm Oil Mill Effluent (POME) by Anaerobic Fermentation Using Clostridium Beijerinckii

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ISBN 13 :
Total Pages : 48 pages
Book Rating : 4.:/5 (953 download)

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Book Synopsis Effect of Time and Temperature on Butanol Production from Palm Oil Mill Effluent (POME) by Anaerobic Fermentation Using Clostridium Beijerinckii by : Siti Hazriah Hamzah

Download or read book Effect of Time and Temperature on Butanol Production from Palm Oil Mill Effluent (POME) by Anaerobic Fermentation Using Clostridium Beijerinckii written by Siti Hazriah Hamzah and published by . This book was released on 2010 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt: Owing to the increasing volume of palm oil mill effluent (POME) wastewater generated, disposal remains as perennial problem and its bioconversion has been considered as an option for pollution control (C.N. Hipolito et al., 2008). Thus, the availability of an inexpensive raw material; POME which will used in this research is essential if solvent fermentation is to become economically viable. In Malaysia, POME represents an alternative cheap carbon source for fermentation processes that is attractive in both economic and geographical considerations. The mainly research purpose is to study the production of butanol from the anareobic fermentation of solventogenic bacteria (clostridium beijerinkii, ATCC 51743) by using POME as the fermentation media. The scope of the research is to study the effect of fermentation time and temperature to the butanol production. The fermentation is based on batch fermentation in schott bottle. It is an anaerobic fermentation and the strain, Clostridium Beijerinckii (ATCC 51743) was treated strictly in an anaerobic condition. Optimum conditions that were maintained in this research are the substrate concentration and agitation rate. POME and Reinforced Clostridia Media (RCM) were used as the growth medium in this batch culture. The fermentation temperature that were used in this study are 35°C, 40°C and 45°C while the fermentation time that were used are 48 to 72 hours respectively. The results indicated that the concentration of butanol will decreases as the temperature increases. The highest concentration of butanol that produced by POME was 0.224 g L-1 at 35°C by 72 hours of fermentation. From this study, it is showed that POME can produce butanol at optimum fermentation temperature at 35°C in 72 hours of fermentation time. Hence, the result also showed that POME is viable to use as growth medium to produce butanol.

Advances in Gram-Positive Endospore-Forming Bacteria Research and Application: 2013 Edition

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Publisher : ScholarlyEditions
ISBN 13 : 1481670573
Total Pages : 99 pages
Book Rating : 4.4/5 (816 download)

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Book Synopsis Advances in Gram-Positive Endospore-Forming Bacteria Research and Application: 2013 Edition by :

Download or read book Advances in Gram-Positive Endospore-Forming Bacteria Research and Application: 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 99 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Gram-Positive Endospore-Forming Bacteria Research and Application: 2013 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about ZZZAdditional Research in a concise format. The editors have built Advances in Gram-Positive Endospore-Forming Bacteria Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about ZZZAdditional Research in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Gram-Positive Endospore-Forming Bacteria Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Developments in Sustainable Chemical and Bioprocess Technology

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Publisher : Springer Science & Business Media
ISBN 13 : 1461462088
Total Pages : 405 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Developments in Sustainable Chemical and Bioprocess Technology by : Ravindra Pogaku

Download or read book Developments in Sustainable Chemical and Bioprocess Technology written by Ravindra Pogaku and published by Springer Science & Business Media. This book was released on 2013-11-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Environmental sustainability and development is of critical importance. Technological advances in the production of new energy sources are making their way into our lives in more and more depth every day. However, there is an urgent need to address the technological challenges and advancement of the various chemical and bio-processes to maintain the dynamic sustainability of our energy needs. Toward that end, an attempt is being made to look at recent advances, key issues still faced and where possible, offer suggestions on alternative technologies to optimize sustainable processes. Still considered a new area of science, energy sources themselves are still being 'discovered'...meaning, what is financially viable in the current marketplace is changing. For example, energy from plants has not been financially viable in the past because of the high cost of growing, harvesting, breaking down cell walls, disposal of waste products, etc. Materials used to derive energy from sustainable resources is changing, making previously high-cost processes more efficient. It is crucial that the industry as a while works in tandem to develop crops that new technological advances make financially feasible. This book will cover recent advances in the chemicals, bioprocesses and other materials used in growing and extracting energy from sustainable products. Membrane/cell wall digestion issues will also be covered as well as recovering mamixal amounts of energy from sources to limit waste. Finally a section on safety and control will be presented with has been poorly covered in other publications. ​

Application of Factorial Design in Enhanced Bio-Butanol Production from Corn Stover Fermentation

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ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (134 download)

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Book Synopsis Application of Factorial Design in Enhanced Bio-Butanol Production from Corn Stover Fermentation by : Dylan M. LaFerriere

Download or read book Application of Factorial Design in Enhanced Bio-Butanol Production from Corn Stover Fermentation written by Dylan M. LaFerriere and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this study acetone, butanol and ethanol (ABE) production was attempted from Corn Stover in small cultures using Clostridium Beijerinckii NCIMB 8052. Prior experiments using C. Beijerinckii have yielded ABE solvents in a range of 14.6g/L to approximately 165.1g/L (conducted in fed batch cultures); With about 60% of solution being butanol. From this study, we successfully produced n-butanol, and ethanol from corn stover fermentation. We produced approximately 4mL of butanol and 1mL of ethanol from a 1L (86g) corn stover solution. We had a productivity of about 3.37% (m/m) n-butanol. Through Gas chromatography and Infrared Spectroscopy, we could identify and confirm the presence of butanol and ethanol from fermentation. We then applied the idea of factorial design to the experiment to identify key factors influencing bio-butanol production from fermentation of agricultural waste. With a holistic approach, we attempted to identify the effects of bacterial strain and pH of the system on production of biofuels. Previous research has shown that two key clostridium strains have been able to successfully digest yeast by-products from agricultural waste, and through fermentation, produce bio-fuels. However, upon analyses of data provided by Minitab, and the application of factorial design, we found no clear correlation between factors of bacterial strain and/or pH. Bio-butanol production is not dependent on either factor. Therefore, we can conclude that further research in butanol production through fermentation of agricultural waste is needed. It would be ideal to consider factors external to bacterial strain that may influence the successful digestion and fermentation of yeast extracted from agricultural waste --

Microbial Biotechnology in Horticulture

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Publisher : CRC Press
ISBN 13 : 1482280434
Total Pages : 566 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Microbial Biotechnology in Horticulture by : R C Ray

Download or read book Microbial Biotechnology in Horticulture written by R C Ray and published by CRC Press. This book was released on 2006-01-05 with total page 566 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of microbial biotechnology to horticulture is of great importance, because it has the potential to increase productivity, to enhance quality and shelf-life of the produce and to develop novel techniques in food processing and for conversion of horticultural wastes into renewal energy sources. In addition, a wide array of scientific

Study of the Continuous Acetone-butanol-ethanol (ABE) Fermentation Using Immobilized Clostridium Aceto-butylicum

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ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.:/5 (244 download)

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Book Synopsis Study of the Continuous Acetone-butanol-ethanol (ABE) Fermentation Using Immobilized Clostridium Aceto-butylicum by : Rifatul Widjhati

Download or read book Study of the Continuous Acetone-butanol-ethanol (ABE) Fermentation Using Immobilized Clostridium Aceto-butylicum written by Rifatul Widjhati and published by . This book was released on 1990 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Acetone-butanol-ethanol Fermentation and Pervaporation by Clostridium Acetobutylicum B18

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ISBN 13 :
Total Pages : 372 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Acetone-butanol-ethanol Fermentation and Pervaporation by Clostridium Acetobutylicum B18 by : Qinghuang Geng

Download or read book Acetone-butanol-ethanol Fermentation and Pervaporation by Clostridium Acetobutylicum B18 written by Qinghuang Geng and published by . This book was released on 1995 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Patterns of Acetone-butanol-ethanol (ABE) Fermentation from NYPA (Nypa Fruticans Wurmb) Juide

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ISBN 13 :
Total Pages : 3 pages
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Book Synopsis Patterns of Acetone-butanol-ethanol (ABE) Fermentation from NYPA (Nypa Fruticans Wurmb) Juide by : E. Gumbira-Sa'id

Download or read book Patterns of Acetone-butanol-ethanol (ABE) Fermentation from NYPA (Nypa Fruticans Wurmb) Juide written by E. Gumbira-Sa'id and published by . This book was released on 1994 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt: As one of the most potential non-conventional fermentable sugar sources, Nypa Juice is suitable for acetone-butanol-ethnol (ABE) fermentation. Using Clostridium acetobutylicum P262 and Nypa juice, 14.07, 2.59 and 0.79 g/l butanol, acetone and ethanol were produced during 88 h of fermentation. [Authors' abstract].

Improvement of Fermentation of Dried Distillers' Grains and Solubles (DDGS) Hydrolysates to Acetone Butanol and Ethanol (ABE) with Hydrolysate-adapted Clostridium Beijerinckii BA 101

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Book Rating : 4.:/5 (71 download)

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Book Synopsis Improvement of Fermentation of Dried Distillers' Grains and Solubles (DDGS) Hydrolysates to Acetone Butanol and Ethanol (ABE) with Hydrolysate-adapted Clostridium Beijerinckii BA 101 by :

Download or read book Improvement of Fermentation of Dried Distillers' Grains and Solubles (DDGS) Hydrolysates to Acetone Butanol and Ethanol (ABE) with Hydrolysate-adapted Clostridium Beijerinckii BA 101 written by and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: