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Probing Translational Control During Drosophila Oogenesi
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Book Synopsis Probing Translational Control During Drosophila Oogenesi by : Yingshi Peng
Download or read book Probing Translational Control During Drosophila Oogenesi written by Yingshi Peng and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Translational control provides a key mechanism for the spatial and temporal regulation of eukaryotic gene expression. It plays a particularly important role in early embryonic development in organisms that rely heavily on maternally supplied mRNAs. The Drosophila developing oocyte serves as a valuable model for studying translational control. The Drosophila posterior determinant, nanos (nos), is translationally repressed throughout the oocyte cytoplasm except at the posterior pole. A multi-functional RNA-binding protein Glorund (Glo), the homolog of the mammalian hnRNP F/H family of proteins, represses translation of nos during oogenesis by targeting both translation initiation and translation elongation. To elucidate the molecular mechanism by which Glo regulates nos, I identified dFMRP as a Glo-interacting protein. By biochemically dissecting repression of nos translation in vitro, I demonstrated that dFMRP specifically inhibits translation elongation. Furthermore, I combined mutational analysis and in vivo and in vitro binding assays to show that Glo's qRRM2 domain specifically and directly interacts with dFMRP, suggesting that Glo's RNA-binding domains can also function as protein-protein interaction interfaces critical for its regulatory functions. Additionally, I applied ribosome footprint profiling to the Drosophila ovary at different developmental stages to identify maternal transcripts regulated during the translation elongation phase. A footprint peak-finding tool has been developed to detect transcriptome-wide ribosome stalling sites. My preliminary results suggest that developmentally regulated ribosome stalling sites may be widely present on Drosophila maternal transcripts. An A-/P-site mapping algorithm is currently under development to facilitate mechanistic analysis of ribosome stalling during oogenesis.
Book Synopsis Translational Regulation of Oskar Mrna During Drosophila Oogenesis by : Yung S. Lie
Download or read book Translational Regulation of Oskar Mrna During Drosophila Oogenesis written by Yung S. Lie and published by . This book was released on 2000 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Translational control of oskar mRNA during oogenesis in Drosophila melanogaster by : Stefania Castagnetti
Download or read book Translational control of oskar mRNA during oogenesis in Drosophila melanogaster written by Stefania Castagnetti and published by . This book was released on 2001 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Translational Control of Protein Synthesis in Drosophila by : Matthew Peter Scott
Download or read book Translational Control of Protein Synthesis in Drosophila written by Matthew Peter Scott and published by . This book was released on 1980 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Translation Control Tunes Drosophila Oogenesis by : Elliot T. Martin
Download or read book Translation Control Tunes Drosophila Oogenesis written by Elliot T. Martin and published by . This book was released on 2022 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis TCE-mediated Translational Control and TCE Function During Drosophila Development by : Heather Keryn Duchow
Download or read book TCE-mediated Translational Control and TCE Function During Drosophila Development written by Heather Keryn Duchow and published by . This book was released on 2003 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Translational Control Mechanisms in Drosophila by : Sara Elizabeth Perlaky
Download or read book Translational Control Mechanisms in Drosophila written by Sara Elizabeth Perlaky and published by . This book was released on 1998 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Translational Control in Drosophila Development by : Marshal Evan Lieberfarb
Download or read book Translational Control in Drosophila Development written by Marshal Evan Lieberfarb and published by . This book was released on 1996 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Comparative Methods Approach to the Study of Oocytes and Embryos by : Joel D. Richter
Download or read book A Comparative Methods Approach to the Study of Oocytes and Embryos written by Joel D. Richter and published by Oxford University Press, USA. This book was released on 1999-01 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past twenty years, biologists have made remarkable progress in understanding each step as a single fertilized egg develops into a mature organism, and the research draws on a tremendous array of experimental techniques. A Comparative Methods Approach to the Study of Oocytes and Embryos takes a unique approach to the field, providing a hands-on guide to three of the most important experimental subjects--frogs, fruitflies, and mice. By collecting reliable techniques in a single volume, the book is both an invaluable resource for new researchers and an ideal platform for comparing these seemingly disparate biological systems. The volume contains work from many leaders in the field and spans research in genetics, molecular and cellular biology, and morphology. It includes methods for oocyte and embryo culture, microinjection, in situ hybridization, immunohistochemistry, time-lapse microscopy, intracellular ion measurements, and embryonic dissections.
Book Synopsis Translational Control of Gene Expression by : Nahum Sonenberg
Download or read book Translational Control of Gene Expression written by Nahum Sonenberg and published by CSHL Press. This book was released on 2001 with total page 1034 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control.
Book Synopsis S-adenosylmethionine-dependent Methyltransferases: Structures And Functions by : Robert M Blumenthal
Download or read book S-adenosylmethionine-dependent Methyltransferases: Structures And Functions written by Robert M Blumenthal and published by World Scientific. This book was released on 1999-07-23 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: This invaluable volume, written by an international group of scientists, presents an overview of the AdoMet-dependent methyltransferases, with special emphasis on structure-function relationships.S-adenosyl-L-methionine (AdoMet) is the second most commonly used enzyme cofactor after ATP. The AdoMet-dependent methyltransferases act on a wide variety of target molecules, including DNA, RNA, protein, polysaccharides, lipids and a range of small molecules.The well-conserved architecture of these enzymes, and the implications of this conservation for their evolutionary history, are major themes of this book. The thirteen chapters describe in detail the structures, enzyme kinetics and biological roles of the AdoMet-dependent methyltransferases from a wide range of cell types: plant, animal, bacterial and archaeal.
Download or read book Vectors written by Raymond L. Rodriguez and published by Butterworth-Heinemann. This book was released on 2014-05-20 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.
Download or read book Oocytes written by Malgorzata Kloc and published by Springer. This book was released on 2017-08-02 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book combines the most recent knowledge on the maternal, i.e. oocyte/egg-specific, molecules and processes. The volume covers the most recent advances in a plethora of subjects such as: maternal transfer of immunity, localized RNAs functions and mechanisms of RNA localization, transcriptional repression of maternal messages, maternal inheritance and maternal role of CRISPR/Cas9-based genome editing, chromatin remodeling and epigenetic modifications, maternal function of nucleosomes, maternal mitochondria and energy supply, role of bacterial symbionts and their maternal transmission, acquisition of oocyte polarity and evolution of maternal effect genes, germ plasm and oosome origin and functions, mechanisms of oocyte activation and soma germ cells communication. Currently, no other book on the market combines such a comprehensive list of subjects in one volume. Moreover, the information provided is a cross-section through oocytes from various invertebrate and vertebrate species, which is another unique feature of this book. The readers, therefore, get a completely new and invaluable perspective on all covered subjects.
Book Synopsis Germline Stem Cells by : Michael Buszczak
Download or read book Germline Stem Cells written by Michael Buszczak and published by Humana. This book was released on 2016-10-13 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.
Book Synopsis Maternal Control of Development in Vertebrates by : Florence Louise Marlow
Download or read book Maternal Control of Development in Vertebrates written by Florence Louise Marlow and published by Morgan & Claypool Publishers. This book was released on 2010 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eggs of all animals contain mRNAs and proteins that are supplied to or deposited in the egg as it develops during oogenesis. These maternal gene products regulate all aspects of oocyte development, and an embryo fully relies on these maternal gene products for all aspects of its early development, including fertilization, transitions between meiotic and mitotic cell cycles, and activation of its own genome. Given the diverse processes required to produce a developmentally competent egg and embryo, it is not surprising that maternal gene products are not only essential for normal embryonic development but also for fertility. This review provides an overview of fundamental aspects of oocyte and early embryonic development and the interference and genetic approaches that have provided access to maternally regulated aspects of vertebrate development. Some of the pathways and molecules highlighted in this review, in particular, Bmps, Wnts, small GTPases, cytoskeletal components, and cell cycle regulators, are well known and are essential regulators of multiple aspects of animal development, including oogenesis, early embryogenesis, organogenesis, and reproductive fitness of the adult animal. Specific examples of developmental processes under maternal control and the essential proteins will be explored in each chapter, and where known conserved aspects or divergent roles for these maternal regulators of early vertebrate development will be discussed throughout this review. Table of Contents: Introduction / Oogenesis: From Germline Stem Cells to Germline Cysts / Oocyte Polarity and the Embryonic Axes: The Balbiani Body, an Ancient Oocyte Asymmetry / Preparing Developmentally Competent Eggs / Egg Activation / Blocking Polyspermy / Cleavage/ Mitosis: Going Multicellular / Maternal-Zygotic Transition / Reprogramming: Epigenetic Modifications and Zygotic Genome Activation / Dorsal-Ventral Axis Formation before Zygotic Genome Activation in Zebrafish and Frogs / Maternal TGF-β and the Dorsal-Ventral Embryonic Axis / Maternal Control After Zygotic Genome Activation / Compensation by Stable Maternal Proteins / Maternal Contributions to Germline Establishment or Maintenance / Perspective / Acknowledgments / References
Book Synopsis Scientific Frontiers in Developmental Toxicology and Risk Assessment by : National Research Council
Download or read book Scientific Frontiers in Developmental Toxicology and Risk Assessment written by National Research Council and published by National Academies Press. This book was released on 2000-12-21 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
Book Synopsis Translation Mechanisms by : Jacques Lapointe
Download or read book Translation Mechanisms written by Jacques Lapointe and published by Springer Science & Business Media. This book was released on 2003-07-31 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Translation Mechanisms provides investigators and graduate students with overviews of recent developments in the field of protein biosynthesis that are fuelled by the explosive and synergic growth of structural biology, genomics, and bioinformatics. The outstanding progress in our understanding of the structure, dynamics, and evolution of the prokaryotic and eukaryotic translation machinery, as well as applications in medicine and biotechnology, are described in 26 chapters covering recent discoveries on: -the subtleties of tRNA aminoacylation with natural and unnatural amino acids. -the control of mRNA stability, a key step of gene regulation. -ribosome structure and function, in the era of the atomic-crystal resolution of the ribosome. -the regulation of the biosynthesis of the translational machinery components. -the action of a variety of inhibitors of translation and the prospect for clinical studies.
