Principles Of Animal Cell Culture

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Culture of Animal Cells

Author : R. Ian Freshney
Publisher : Wiley-Liss
ISBN 13 : 9780471589662
Total Pages : 510 pages
Book Rating : 4.5/5 (896 download)

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Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 1993-12-29 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Animal Cell Culture Methods

Author :
Publisher : Academic Press
ISBN 13 : 0080859550
Total Pages : 389 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Animal Cell Culture Methods by :

Download or read book Animal Cell Culture Methods written by and published by Academic Press. This book was released on 1998-07-03 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.Key Features* Reviews basic principles of cell culture* Gives options and techniques on how to look at cells

Principles and Practice of Animal Tissue Culture (Second Edition)

Author :
Publisher : Universities Press
ISBN 13 : 8173717192
Total Pages : 248 pages
Book Rating : 4.1/5 (737 download)

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Book Synopsis Principles and Practice of Animal Tissue Culture (Second Edition) by :

Download or read book Principles and Practice of Animal Tissue Culture (Second Edition) written by and published by Universities Press. This book was released on with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Animal Cell Culture and Technology

Author : Michael Butler
Publisher : Garland Science
ISBN 13 : 1135325375
Total Pages : 299 pages
Book Rating : 4.1/5 (353 download)

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Book Synopsis Animal Cell Culture and Technology by : Michael Butler

Download or read book Animal Cell Culture and Technology written by Michael Butler and published by Garland Science. This book was released on 2004-08-02 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Animal Cell Bioreactors

Author : Chester S. Ho
Publisher : Butterworth-Heinemann
ISBN 13 : 1483289478
Total Pages : 511 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Animal Cell Bioreactors by : Chester S. Ho

Download or read book Animal Cell Bioreactors written by Chester S. Ho and published by Butterworth-Heinemann. This book was released on 2013-10-22 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. It addresses engineering aspects such as mass transfer, instrumentation, and control ensuring successful design and operation of animal cell bioreactors. The goal is to provide a comprehensive analysis and review in the advancement of the bioreactor systems for large-scale animal cell cultures. The book is organized into four parts. Part I traces the historical development of animal cell biotechnology. It presents examples of work in progress that seeks to make animal cell biotechnology processes as productive on a cost per unit of product basis as that achieved by other microbial systems. Part II includes chapters dealing with the implications of cell biology in animal cell biotechnology; protein-bound oligosaccharides and their structures; the development of serum-free media and its use in the production of biologically active substances; and the metabolism of mammalian cells. Part III focuses on animal cell cultivation, covering topics such as the fixed bed immobilized culture; three-dimensional microcarriers; and hydrodynamic phenomena in microcarrier cultures. Part IV discusses the design, operation, and control of animal cell bioreactors.

Medicines from Animal Cell Culture

Author : Glyn N. Stacey
Publisher : John Wiley & Sons
ISBN 13 : 9780470723784
Total Pages : 696 pages
Book Rating : 4.7/5 (237 download)

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Book Synopsis Medicines from Animal Cell Culture by : Glyn N. Stacey

Download or read book Medicines from Animal Cell Culture written by Glyn N. Stacey and published by John Wiley & Sons. This book was released on 2007-06-29 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicines from Animal Cell Culture focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells. Medicines from Animal Cell Culture: Provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterisation of both the cells and the derived products Describes the preparation of stem cells and others for use in cell-based therapies – an area of burgeoning research Includes experimental examples to indicate expected results Covers regulatory issues from the UK, the EU and the USA and reviews how these are developing around the world Addresses the key issues of standardisation and validation with chapters on GLP and GMP for cell culture processes Delivering insight into the exciting world of biological medicines and directions for further investigation into specific topics, Medicines from Animal Cell Culture is an essential resource for researchers and technicians at all levels using cell culture within the pharmaceutical, biotechnology and biomedical industries. It is of value to laboratory managers in these industries and to all those interested in this topic alike.

Principles Of Animal Cell Culture: Student Compendium. Textbook Student Edition

Author : Sinha Basant K.
Publisher : IBDC Publishers
ISBN 13 : 9788181892485
Total Pages : 310 pages
Book Rating : 4.8/5 (924 download)

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Book Synopsis Principles Of Animal Cell Culture: Student Compendium. Textbook Student Edition by : Sinha Basant K.

Download or read book Principles Of Animal Cell Culture: Student Compendium. Textbook Student Edition written by Sinha Basant K. and published by IBDC Publishers. This book was released on 2008 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Animal Cell Technology

Author : Leda Castilho
Publisher : Garland Science
ISBN 13 : 0203895169
Total Pages : 506 pages
Book Rating : 4.2/5 (38 download)

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Book Synopsis Animal Cell Technology by : Leda Castilho

Download or read book Animal Cell Technology written by Leda Castilho and published by Garland Science. This book was released on 2008-03-01 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.

Animal Cell Biotechnology

Author : Hansjörg Hauser
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110278960
Total Pages : 718 pages
Book Rating : 4.1/5 (12 download)

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Book Synopsis Animal Cell Biotechnology by : Hansjörg Hauser

Download or read book Animal Cell Biotechnology written by Hansjörg Hauser and published by Walter de Gruyter GmbH & Co KG. This book was released on 2014-11-10 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Principles and Techniques of Biochemistry and Molecular Biology

Author : Keith Wilson
Publisher : Cambridge University Press
ISBN 13 : 0521516358
Total Pages : 769 pages
Book Rating : 4.5/5 (215 download)

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Book Synopsis Principles and Techniques of Biochemistry and Molecular Biology by : Keith Wilson

Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Cell Culture Engineering

Author : Gyun Min Lee
Publisher : John Wiley & Sons
ISBN 13 : 3527343342
Total Pages : 436 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Cell Culture Engineering by : Gyun Min Lee

Download or read book Cell Culture Engineering written by Gyun Min Lee and published by John Wiley & Sons. This book was released on 2020-01-13 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

The AGT Cytogenetics Laboratory Manual

Author : Marilyn S. Arsham
Publisher : John Wiley & Sons
ISBN 13 : 1119061229
Total Pages : 1216 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis The AGT Cytogenetics Laboratory Manual by : Marilyn S. Arsham

Download or read book The AGT Cytogenetics Laboratory Manual written by Marilyn S. Arsham and published by John Wiley & Sons. This book was released on 2017-04-24 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.

Animal Cell Culture

Author : R. Ian Freshney
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 358 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Animal Cell Culture by : R. Ian Freshney

Download or read book Animal Cell Culture written by R. Ian Freshney and published by Oxford University Press, USA. This book was released on 1992 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.

Introduction to Cell and Tissue Culture

Author : Jennie P. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 0585275718
Total Pages : 247 pages
Book Rating : 4.5/5 (852 download)

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Book Synopsis Introduction to Cell and Tissue Culture by : Jennie P. Mather

Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Animal Cell Culture

Author : Mohamed Al-Rubeai
Publisher : Springer
ISBN 13 : 3319103202
Total Pages : 766 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Animal Cell Culture by : Mohamed Al-Rubeai

Download or read book Animal Cell Culture written by Mohamed Al-Rubeai and published by Springer. This book was released on 2014-11-28 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Engineering Principles in Biotechnology

Author : Wei-Shou Hu
Publisher : John Wiley & Sons
ISBN 13 : 1119159024
Total Pages : 502 pages
Book Rating : 4.1/5 (191 download)

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Book Synopsis Engineering Principles in Biotechnology by : Wei-Shou Hu

Download or read book Engineering Principles in Biotechnology written by Wei-Shou Hu and published by John Wiley & Sons. This book was released on 2017-11-13 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a short introduction to the engineering principles of harnessing the vast potential of microorganisms, and animal and plant cells in making biochemical products. It was written for scientists who have no background in engineering, and for engineers with minimal background in biology. The overall subject dealt with is process. But the coverage goes beyond the process of biomanufacturing in the bioreactor, and extends to the factory of cell’s biosynthetic machinery. Starting with an overview of biotechnology and organism, engineers are eased into biochemical reactions and life scientists are exposed to the technology of production using cells. Subsequent chapters allow engineers to be acquainted with biochemical pathways, while life scientist learn about stoichiometric and kinetic principles of reactions and cell growth. This leads to the coverage of reactors, oxygen transfer and scale up. Following three chapters on biomanufacturing of current and future importance, i.e. cell culture, stem cells and synthetic biology, the topic switches to product purification, first with a conceptual coverage of operations used in bioseparation, and then a more detailed analysis to provide a conceptual understanding of chromatography, the modern workhorse of bioseparation. Drawing on principles from engineering and life sciences, this book is for practitioners in biotechnology and bioengineering. The author has used the book for a course for advanced students in both engineering and life sciences. To this end, problems are provided at the end of each chapter.