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Author : Yongming Li
Publisher : Ios PressInc
ISBN 13 : 9789051993172
Total Pages : 312 pages
Book Rating : 4.9/5 (931 download)
Download or read book Practical Protocols in Molecular Biology written by Yongming Li and published by Ios PressInc. This book was released on 1997-01-01 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Dario Leister
Publisher : Springer Science & Business Media
ISBN 13 : 1588296679
Total Pages : 1195 pages
Book Rating : 4.5/5 (882 download)
Download or read book Mitochondria written by Dario Leister and published by Springer Science & Business Media. This book was released on 2007-06-12 with total page 1195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.
Author : Khalid Z. Masoodi
Publisher : Academic Press
ISBN 13 : 012824450X
Total Pages : 220 pages
Book Rating : 4.1/5 (282 download)
Download or read book Advanced Methods in Molecular Biology and Biotechnology written by Khalid Z. Masoodi and published by Academic Press. This book was released on 2020-10-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author : Cheryl D. Helgason
Publisher : Humana Press
ISBN 13 : 9781493956524
Total Pages : 550 pages
Book Rating : 4.9/5 (565 download)
Download or read book Basic Cell Culture Protocols written by Cheryl D. Helgason and published by Humana Press. This book was released on 2016-08-23 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
Author : Robert J. Slater
Publisher : Springer Science & Business Media
ISBN 13 : 1603274057
Total Pages : 385 pages
Book Rating : 4.6/5 (32 download)
Download or read book Experiments in Molecular Biology written by Robert J. Slater and published by Springer Science & Business Media. This book was released on 2008-02-07 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research in the field of molecular biology has progressed at a fascinating rate in recent years. Much of this progress results from the development of new laboratory techniques that allow very precise fractionation and analysis of nucleic acids and proteins, as well as the construction of recom binant DNA molecules that can then be cloned and expressed in host cells. Progress has been so rapid that there has been a shortfall in the training of appropriately qualified staff. Many existing laboratory workers require retraining, and many educational institutions have had difficulty incor porating the new molecular biology techniques into their teaching programs. Although there are several manuals currently available that describe laboratory techniques in molecular biology, they are principally written for the indivi dual research worker and are not intended for use in the design of practical classes for students. The aim of this book is to provide just such a series of pro tocols for the teaching of practical molecular biology. The idea arose following the success of several Workshops in Molecular Biology, organized and taught by staff in the Biol ogy Department of the Hatfield Polytechnic. Gradually, the protocols used in the workshops have been incorporated into the Hatfield undergraduate and postgraduate teaching pro grams and have now been collected together to form a book.
Author : Lucília Domingues
Publisher : Springer Nature
ISBN 13 : 1071633589
Total Pages : 255 pages
Book Rating : 4.0/5 (716 download)
Download or read book PCR written by Lucília Domingues and published by Springer Nature. This book was released on 2023-09-23 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Author : Sandeep Singh
Publisher : Bentham Science Publishers
ISBN 13 : 981143929X
Total Pages : 186 pages
Book Rating : 4.8/5 (114 download)
Download or read book Protocols used in Molecular Biology written by Sandeep Singh and published by Bentham Science Publishers. This book was released on 2020-01-23 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Author : Frederick M. Ausubel
Publisher :
ISBN 13 :
Total Pages : 854 pages
Book Rating : 4.:/5 (318 download)
Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 1992 with total page 854 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Terence A. Brown
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 328 pages
Book Rating : 4.3/5 (91 download)
Download or read book Essential Molecular Biology written by Terence A. Brown and published by Oxford University Press, USA. This book was released on 1991 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to practical molecular biology aimed specifically at students and other research workers who have no prior experience of the subject. This first of two volumes describes fundamental techniques for handling and cloning DNA.
Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.6/5 (425 download)
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author : Dilip Kumar Arora
Publisher : Springer
ISBN 13 : 9783662509678
Total Pages : 0 pages
Book Rating : 4.5/5 (96 download)
Download or read book Analyzing Microbes written by Dilip Kumar Arora and published by Springer. This book was released on 2016-09-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.
Author : H. N. Thatoi
Publisher :
ISBN 13 : 9789385909160
Total Pages : 0 pages
Book Rating : 4.9/5 (91 download)
Download or read book Practical Biotechnology written by H. N. Thatoi and published by . This book was released on 2017-09-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces the different tools and methods of molecular biology from both a theoretical and practical perspective. Discusses the principles and procedures, their potential and drawbacks, involved in experiments in laboratories. Provides information on safety guidelines, ethical issues, genetic engineering work and laboratory set-ups. The books is aimed at advanced students, as well as research scientists and technicians.
Author : Adrian J. Harwood
Publisher :
ISBN 13 :
Total Pages : 534 pages
Book Rating : 4.3/5 (91 download)
Download or read book Basic DNA and RNA Protocols written by Adrian J. Harwood and published by . This book was released on 1996-02-19 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Basic DNA and RNA Protocols experienced researchers and innovators present an essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. Its outstanding, thoroughly up-to-date, and eminently practical protocols make it an essential tool for today's molecular biologist.
Author : Jonathan E. Hempel
Publisher : Humana
ISBN 13 : 9781493922680
Total Pages : 0 pages
Book Rating : 4.9/5 (226 download)
Download or read book Chemical Biology written by Jonathan E. Hempel and published by Humana. This book was released on 2015-01-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.
Author : Shafat A. Latoo
Publisher :
ISBN 13 : 9781685078621
Total Pages : pages
Book Rating : 4.0/5 (786 download)
Download or read book Laboratory Guide: Concepts and Protocols for Practical Courses in Biochemistry and Molecular Biology written by Shafat A. Latoo and published by . This book was released on 2022-09 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Guide: Concepts and Protocols for Practical Courses in Biochemistry and Molecular Biology communicates science to novice and advanced learners and teaches students about basic concepts and their practical applications in the laboratory. By understanding and properly applying the protocols included in this book, students will be able to complete their laboratory-based courses with ease.
Author : Pat Vaughan
Publisher : Springer Science & Business Media
ISBN 13 : 1592590683
Total Pages : 210 pages
Book Rating : 4.5/5 (925 download)
Download or read book DNA Repair Protocols written by Pat Vaughan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine iden- fied the collective DNA repair systems as its “Molecule of the Year” in 1994.
Author : P. V. G. K. Sarma
Publisher : MJP Publisher
ISBN 13 :
Total Pages : 228 pages
Book Rating : 4./5 ( download)
Download or read book MOLECULAR BIOLOGY written by P. V. G. K. Sarma and published by MJP Publisher. This book was released on 2019-06-05 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. DNA Isolation 2. Electrophoresis 3. Isolation of total RNA 4. Restriction Endonucleases 5. Enzymes Used in the DNA Manipulation Techniques 6. Hybridization Techniques 7. Polymerase Chain Reaction (PCR) 8. DNA Sequencing Techniques
