Practical Protein Electrophoresis for Genetic Research

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Publisher :
ISBN 13 :
Total Pages : 140 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Practical Protein Electrophoresis for Genetic Research by : George Acquaah

Download or read book Practical Protein Electrophoresis for Genetic Research written by George Acquaah and published by . This book was released on 1992 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book enables the novice to understand the "whys" and "hows" of electrophoresis and to initiate and complete an electrophoretic investigation from beginning laboratory organization to publishing results.

Protein Electrophoresis

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Publisher : Humana Press
ISBN 13 : 9781493962419
Total Pages : 664 pages
Book Rating : 4.9/5 (624 download)

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Book Synopsis Protein Electrophoresis by : Biji T. Kurien

Download or read book Protein Electrophoresis written by Biji T. Kurien and published by Humana Press. This book was released on 2016-05-01 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each chapter in this Methods in Molecular Biology book details a specific electrophoretic variant, so that scientists can perform new techniques without difficulty. Includes step-by-step protocols, troubleshooting advice and tips on avoiding pitfalls."

Gel Electrophoresis of Proteins

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Publisher : OUP Oxford
ISBN 13 : 0191565636
Total Pages : 378 pages
Book Rating : 4.1/5 (915 download)

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Book Synopsis Gel Electrophoresis of Proteins by : B. D. Hames

Download or read book Gel Electrophoresis of Proteins written by B. D. Hames and published by OUP Oxford. This book was released on 1998-10-01 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.

Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

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Publisher : Springer Science & Business Media
ISBN 13 : 9783540657927
Total Pages : 272 pages
Book Rating : 4.6/5 (579 download)

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Book Synopsis Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods by : T. Rabilloud

Download or read book Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods written by T. Rabilloud and published by Springer Science & Business Media. This book was released on 1999-09-17 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.

Electrophoretic Separation of Proteins

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Publisher :
ISBN 13 : 9781493987931
Total Pages : 523 pages
Book Rating : 4.9/5 (879 download)

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Book Synopsis Electrophoretic Separation of Proteins by : Biji T. Kurien

Download or read book Electrophoretic Separation of Proteins written by Biji T. Kurien and published by . This book was released on with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Gel Detection and Imaging

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Publisher : Humana
ISBN 13 : 9781493993819
Total Pages : 289 pages
Book Rating : 4.9/5 (938 download)

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Book Synopsis Protein Gel Detection and Imaging by : Biji T. Kurien

Download or read book Protein Gel Detection and Imaging written by Biji T. Kurien and published by Humana. This book was released on 2019-08-24 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.

Capillary Electrophoresis of Proteins and Peptides

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Publisher :
ISBN 13 : 9781493940141
Total Pages : 234 pages
Book Rating : 4.9/5 (41 download)

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Book Synopsis Capillary Electrophoresis of Proteins and Peptides by : Nguyet Thuy Tran

Download or read book Capillary Electrophoresis of Proteins and Peptides written by Nguyet Thuy Tran and published by . This book was released on 2016 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study."--OCLC.

Analysis of Protein Electrophoresis Gel Images

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Publisher :
ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.:/5 (647 download)

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Book Synopsis Analysis of Protein Electrophoresis Gel Images by : Yassin Labyed

Download or read book Analysis of Protein Electrophoresis Gel Images written by Yassin Labyed and published by . This book was released on 2007 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Electrophoresis in Practice

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Publisher : Wiley-Blackwell
ISBN 13 : 9783527303007
Total Pages : 0 pages
Book Rating : 4.3/5 (3 download)

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Book Synopsis Electrophoresis in Practice by : Reiner Westermeier

Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by Wiley-Blackwell. This book was released on 2001-03-21 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is your "Laboratory Guide" for successful electrophoretic separations ! Organized in two parts it gives the reader a thorough presentation of the fundamentals followed by a detailed description of 15 of the most common methods currently in use. The Third Edition now includes the latest developments in 2D-electrophoresis as well as an overview of the proteome analysis methodology. From reviews of the previous editions: ... The rigorous description of each method along with the extensive figures will easily allow the novice to reproduce these methods in the laboratory... The Analyst ... Perhaps the most important point about the book is that all the recipes provided do actually work. ... Journal of Laboratory Medicine ... an excellent book which we recommend greatly and which has not to be missed in any laboratory of cellular and molecular biology which respects it self ! Cellular and Molecular Biology ... As a comprehensive guide to the huge variety of electrophoretic methods now available, this is very good value ... The superb ... troubleshooting appendix almost justifies the price on ist own ... Laboratory Equipment Digest

Protein Function

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Publisher :
ISBN 13 : 9780199636303
Total Pages : 334 pages
Book Rating : 4.6/5 (363 download)

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Book Synopsis Protein Function by : Thomas E. Creighton

Download or read book Protein Function written by Thomas E. Creighton and published by . This book was released on 1997 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: The highly successful original volume, Protein Function: A Practical Approach, has been completely updated and extended to produce this second edition. Nevertheless, the goal remains the same: the present experimental procedures for analysing protein function that are applicable to many proteins and that can be performed in a typical laboratory without the need for specialized equipment. The latest techniques are described in step-by-step detail for protein identification by two-dimensional gel electrophoresis, the expression of cloned genes in E. coli, the preparative folding of proteins, ligand-binding studies, ligand blotting, affinity labelling, and chemical modification of proteins. The assay, purification, and analysis of sequence-specific DNA-binding proteins are also extensively covered, as is the use of chemical cross-linking to probe protein function.

Clinical Applications of Capillary Electrophoresis

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Publisher : Springer Science & Business Media
ISBN 13 : 1592596894
Total Pages : 215 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Clinical Applications of Capillary Electrophoresis by : Stephen M. Palfrey

Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.

Protein Electrophoresis in Clinical Diagnosis

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Publisher : CRC Press
ISBN 13 : 1444113992
Total Pages : 415 pages
Book Rating : 4.4/5 (441 download)

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Book Synopsis Protein Electrophoresis in Clinical Diagnosis by : David Keren

Download or read book Protein Electrophoresis in Clinical Diagnosis written by David Keren and published by CRC Press. This book was released on 2003-09-26 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of High-Resolution Electrophorsesis and Immunofixation 2e, there have been ever-increasing advances in the analyses of proteins, by electrophoresis in particular. Protein Electrophoresis in Clinical Diagnosis shows the changes in both techniques and interpretation, presenting a comprehensive review of serum protein techniques,

DNA Electrophoresis

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Publisher : Humana
ISBN 13 : 9781493959983
Total Pages : 0 pages
Book Rating : 4.9/5 (599 download)

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Book Synopsis DNA Electrophoresis by : Svetlana Makovets

Download or read book DNA Electrophoresis written by Svetlana Makovets and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Handbook of Detection of Enzymes on Electrophoretic Gels

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Publisher : CRC Press
ISBN 13 : 100065415X
Total Pages : 1137 pages
Book Rating : 4.0/5 (6 download)

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Book Synopsis Handbook of Detection of Enzymes on Electrophoretic Gels by : Gennady P. Manchenko

Download or read book Handbook of Detection of Enzymes on Electrophoretic Gels written by Gennady P. Manchenko and published by CRC Press. This book was released on 2002-12-26 with total page 1137 pages. Available in PDF, EPUB and Kindle. Book excerpt: Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish

Principles of Plant Genetics and Breeding

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Publisher : John Wiley & Sons
ISBN 13 : 1444309013
Total Pages : 584 pages
Book Rating : 4.4/5 (443 download)

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Book Synopsis Principles of Plant Genetics and Breeding by : George Acquaah

Download or read book Principles of Plant Genetics and Breeding written by George Acquaah and published by John Wiley & Sons. This book was released on 2009-03-12 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until recently, plant breeders have depended primarily on classicaltools to develop new and improved products for producers andconsumers. However, with the advent of biotechnology, breeders areincreasingly incorporating molecular tools in their breeding work.In recognition of the current state of methods and theirapplication, this text introduces both classical and moleculartools for plant breeding. Topics such as biotechnology in plant breeding, intellectualproperty, risks, emerging concepts (decentralized breeding, organicbreeding), and more are addressed in this state of the art text.The final 8 chapters provide a useful reference on breeding thelargest and most common crops. In addition, over 25 plant breedersshare their professional experiences while illustrating concepts inthe text. Features include: Comprehensive presentation of both classical and molecularplant breeding tools Industry highlight essays from over 25 professional plantbreeders Chapter introductions, summaries and discussion questions Easy reference glossary Reference chapters on breeding 8 of the largest and most commoncrops Artwork from the book is available to instructors online at ahref="http://www.blackwellpublishing.com/acquaah"www.blackwellpublishing.com/acquaah/a.An Instructor manual CD-ROM for this title is available. Pleasecontact our Higher Education team at ahref="mailto:[email protected]"[email protected]/afor more information.

The Molecular Basis of Plant Genetic Diversity

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Publisher : BoD – Books on Demand
ISBN 13 : 9535101579
Total Pages : 374 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis The Molecular Basis of Plant Genetic Diversity by : Mahmut Caliskan

Download or read book The Molecular Basis of Plant Genetic Diversity written by Mahmut Caliskan and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Molecular Basis of Plant Genetic Diversity presents chapters revealing the magnitude of genetic variations existing in plant populations. Natural populations contain a considerable genetic variability which provides a genomic flexibility that can be used as a raw material for adaptation to changing environmental conditions. The analysis of genetic diversity provides information about allelic variation at a given locus. The increasing availability of PCR-based molecular markers allows the detailed analyses and evaluation of genetic diversity in plants and also, the detection of genes influencing economically important traits. The purpose of the book is to provide a glimpse into the dynamic process of genetic variation by presenting the thoughts of scientists who are engaged in the generation of new ideas and techniques employed for the assessment of genetic diversity, often from very different perspectives. The book should prove useful to students, researchers, and experts in the area of conservation biology, genetic diversity, and molecular biology.

Electrophoresis in Practice

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Publisher : John Wiley & Sons
ISBN 13 : 3527338802
Total Pages : 470 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Electrophoresis in Practice by : Reiner Westermeier

Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-05-16 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.