Practical Flow Cytometry in Haematology

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Publisher : John Wiley & Sons
ISBN 13 : 1118746899
Total Pages : 416 pages
Book Rating : 4.1/5 (187 download)

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Book Synopsis Practical Flow Cytometry in Haematology by : Mike Leach

Download or read book Practical Flow Cytometry in Haematology written by Mike Leach and published by John Wiley & Sons. This book was released on 2015-06-01 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Practical Flow Cytometry in Haematology Diagnosis

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 9780470671207
Total Pages : 0 pages
Book Rating : 4.6/5 (712 download)

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Book Synopsis Practical Flow Cytometry in Haematology Diagnosis by : Mike Leach

Download or read book Practical Flow Cytometry in Haematology Diagnosis written by Mike Leach and published by John Wiley & Sons. This book was released on 2013-04-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

Practical Flow Cytometry

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 0471434035
Total Pages : 736 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro

Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

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Author :
Publisher : Cambridge University Press
ISBN 13 : 1108548830
Total Pages : 248 pages
Book Rating : 4.1/5 (85 download)

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Book Synopsis Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by : Anna Porwit

Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.

Flow Cytometry

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Publisher : Springer Science & Business Media
ISBN 13 : 1597454516
Total Pages : 294 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Flow Cytometry by : Marion G. Macey

Download or read book Flow Cytometry written by Marion G. Macey and published by Springer Science & Business Media. This book was released on 2007-11-03 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Laboratory Hematology Practice

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Publisher : John Wiley & Sons
ISBN 13 : 1444398571
Total Pages : 777 pages
Book Rating : 4.4/5 (443 download)

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Book Synopsis Laboratory Hematology Practice by : Kandice Kottke-Marchant

Download or read book Laboratory Hematology Practice written by Kandice Kottke-Marchant and published by John Wiley & Sons. This book was released on 2012-06-06 with total page 777 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for all those working in the field.

Immunophenotyping for Haematologists

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Publisher : John Wiley & Sons
ISBN 13 : 1119606152
Total Pages : 144 pages
Book Rating : 4.1/5 (196 download)

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Book Synopsis Immunophenotyping for Haematologists by : Barbara J. Bain

Download or read book Immunophenotyping for Haematologists written by Barbara J. Bain and published by John Wiley & Sons. This book was released on 2020-10-13 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers clear and concise instruction on running, reporting and interpreting immunophenotyping studies Written by two well-known haematology educators and experts on the topic, Immunophenotyping for Haematologists contains an introduction to running, reporting and interpreting immunophenotyping studies. The book offers a unique approach to the topic by putting the focus on clinical and laboratory haematologists who are not routinely involved in running and reporting on immunophenotyping studies. Immunophenotyping using flow cytometry has become the method of choice in identifying and sorting cells within complex populations, for example, the analysis of immune or neoplastic cells in a blood sample. The text reviews the purpose and principles of immunophenotyping and includes an introduction and explanation of the principles and the role of immunophenotyping. The authors examine immunophenotypic characteristics of the disease groups commonly encountered and identify the features that differentiate malignant cells from normal cells. To enhance understanding, the book contains multiple choice and extended matching questions which integrates immunophenotyping with clinicopathological features and the results of other investigations to mimic everyday practice. This important book: Provides a concise introduction to running, reporting and interpreting immunophenotyping studies Contains a list of all the antibody specificities currently widely used in diagnosis and disease monitoring Presents an ideal reference for use in laboratories, including immunophenotyping laboratories Aids in the interpretation by covering immunophenotypic characteristics of commonly encountered disease groups Identifies the features that differentiate malignant cells from their normal counterparts Written for haematologists working in both laboratory and clinical haematology, Immunophenotyping for Haematologists is a much-needed reference for understanding and interpreting immunophenotyping studies.

Flow Cytometry of Hematological Malignancies

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 111961130X
Total Pages : 464 pages
Book Rating : 4.1/5 (196 download)

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Book Synopsis Flow Cytometry of Hematological Malignancies by : Claudio Ortolani

Download or read book Flow Cytometry of Hematological Malignancies written by Claudio Ortolani and published by John Wiley & Sons. This book was released on 2021-04-19 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow Cytometry of Hematological Malignancies Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes: Explorations of more than 70 antigens Full-color illustrations throughout New descriptions of recently discovered markers WHO classifications of hematological neoplastic diseases Helpful tips for result interpretation and analysis Featuring all this and more, Flow Cytometry of Hematological Malignancies, Second Edition, is an invaluable resource for both trainee and experienced hematologists, hematopathologists, oncologists, and pathologists, as well as medical students and diagnostic lab technicians.

Dacie and Lewis Practical Haematology E-Book

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Author :
Publisher : Elsevier Health Sciences
ISBN 13 : 0702069256
Total Pages : 600 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Dacie and Lewis Practical Haematology E-Book by : Barbara J. Bain

Download or read book Dacie and Lewis Practical Haematology E-Book written by Barbara J. Bain and published by Elsevier Health Sciences. This book was released on 2016-08-11 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: For more than 65 years, this best-selling text by Drs. Barbara J. Bain, Imelda Bates, and Mike A. Laffan has been the worldwide standard in laboratory haematology. The 12th Edition of Dacie and Lewis Practical Haematology continues the tradition of excellence with thorough coverage of all of the techniques used in the investigation of patients with blood disorders, including the latest technologies as well as traditional manual methods of measurement. You’ll find expert discussions of the principles of each test, possible causes of error, and the interpretation and clinical significance of the findings. A unique section on haematology in under-resourced laboratories. Ideal as a laboratory reference or as a comprehensive exam study tool. Each templated, easy-to-follow chapter has been completely updated, featuring new information on haematological diagnosis, molecular testing, blood transfusion- and much more. Complete coverage of the latest advances in the field. An expanded section on coagulation now covers testing for new anticoagulants and includes clinical applications of the tests.

A History of Haematology

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Publisher : Oxford University Press
ISBN 13 : 0198717601
Total Pages : 247 pages
Book Rating : 4.1/5 (987 download)

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Book Synopsis A History of Haematology by : Shaun R. McCann

Download or read book A History of Haematology written by Shaun R. McCann and published by Oxford University Press. This book was released on 2016 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Blood has long been an object of intrigue for many of the world's philosophers and physicians, and references to it have existed since the earliest studies of human anatomy. Herodotus of Halicarnassus, whose writings 500 years before the birth of Christ drew on stories collected during his widespread travels, was amongst the first to identify the ritualistic and medical significance of blood. However, despite this long established history, haematology as a medical specialty is relatively new. A History of Haematology: From Herodotus to HIV traces the history of haematology from biblical times to the present, discussing the major defining discoveries in the specialty, ranging from war as a catalyst for the development of new techniques in blood transfusion, to the medical response to the HIV/AIDS epidemic. In this beautifully illustrated and passionately rendered history of the field of haematology, Professor Shaun McCann traces the remarkable developments within haematology and the work of the scientists and pioneers central to these advances. This engaging and authoritative history will appeal to a wide audience including haematologists, nurses and other health care workers in haematology, as well as medical students, and general physicians with an interest in haematology.

Diagnostic Techniques in Hematological Malignancies

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Publisher : Cambridge University Press
ISBN 13 : 1139491733
Total Pages : 349 pages
Book Rating : 4.1/5 (394 download)

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Book Synopsis Diagnostic Techniques in Hematological Malignancies by : Wendy N. Erber

Download or read book Diagnostic Techniques in Hematological Malignancies written by Wendy N. Erber and published by Cambridge University Press. This book was released on 2010-11-11 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diagnosis and monitoring of hematological malignancies is complex and requires a systematic approach. Morphology, cell phenotyping, cytogenetics and molecular genetics are essential, and the results must be integrated. Diagnostic Techniques in Hematological Malignancies details the principles and applications of each of these test types in the diagnosis of hematological malignancies in blood and bone marrow. The first section describes the test modalities – including methodological principles, data interpretation and limitations – and is illustrated by clinical examples. The second section focuses on the clinical entities, detailing the most appropriate tests for diagnosis, staging and monitoring of different hematological malignancies and includes test utilization to identify prognostic markers and potential therapeutic targets. With contributions from multiple international experts, this illustrated book is an essential resource for qualified and trainee hematologists, oncologists, and pathologists. It's a practical and useful guide, providing a rational and structured approach to the laboratory assessment of hematological malignancies.

Oxford Handbook of Clinical Haematology

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Publisher : Oxford University Press, USA
ISBN 13 : 019922739X
Total Pages : 881 pages
Book Rating : 4.1/5 (992 download)

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Book Synopsis Oxford Handbook of Clinical Haematology by : Drew Provan

Download or read book Oxford Handbook of Clinical Haematology written by Drew Provan and published by Oxford University Press, USA. This book was released on 2009-02-19 with total page 881 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing essential information needed in clinical practice for the diagnosis and management of patients with blood disorders, this handbook covers haematological investigations and their interpretation, and commonly used protocols.

Flow Cytometry in Hematopathology

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Publisher : Springer Science & Business Media
ISBN 13 : 1592593542
Total Pages : 220 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Flow Cytometry in Hematopathology by : Doyen T. Nguyen

Download or read book Flow Cytometry in Hematopathology written by Doyen T. Nguyen and published by Springer Science & Business Media. This book was released on 2002-11-26 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature.

Diagnostic Hematology

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Publisher : CRC Press
ISBN 13 : 9780750642477
Total Pages : 480 pages
Book Rating : 4.6/5 (424 download)

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Book Synopsis Diagnostic Hematology by : Doyen Nguyen

Download or read book Diagnostic Hematology written by Doyen Nguyen and published by CRC Press. This book was released on 2000-06-05 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic hematology is a problem-solving process involving the gathering and analysis of the relevant findings in each case. Based on the combination of clinical and laboratory data, an experienced diagnostician can formulate the overall 'pattern' of a case. The 'pattern approach' to differential diagnosis is an integral component of the diagnostic reasoning of many expert hematologists. In this unique textbook the authors present a logical and systematic approach to diagnosis, based on the patterns derived from peripheral blood analysis, flow cytometry immunophenotyping studies and bone marrow examination. Unlike standard textbooks which are organized by specific diseases and thereby assume the reader has reached a diagnosis, this text presents the diagnostic problem as it is investigated in practice. The patterns formed by different blood and bone marrow disorders are described in detail and illustrated with color photomicrographs, or flow cytometry graphics. The reader is taken through the process of reaching the differential diagnosis. Advice is given on the proper approach to diagnostic work-up and how to avoid common diagnostic pitfalls. The companion CD-ROM, with full instructions given in the appendix, offers the reader the chance to assess real test results, and see the patterns which lead to the final interpretation in over 200 cases. As a result the reader will develop the skill of pattern recognition and be able to incorporate this diagnostic approach in practice. This is an essential text for physicians practicing hematology and hematopathology, especially those interpreting peripheral blood, flow cytometry and bone marrow specimens; also for technologists and laboratory staff responsible for the screening of full blood counts, the initial review of blood/bone marrow films or immunophenotyping data. System requirements: Windows 95, Windows 98 or Windows NT. Screen resolution of 800 x 600 with 16 million colours (24-bit). Minimum display: at least 65,000 colours (16-bit).

Flow Cytometry and Cell Sorting

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Publisher : Springer Science & Business Media
ISBN 13 : 3662041294
Total Pages : 365 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Flow Cytometry and Cell Sorting by : Andreas Radbruch

Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Flow Cytometry in Neoplastic Hematology

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Author :
Publisher : CRC Press
ISBN 13 : 9781841847023
Total Pages : 0 pages
Book Rating : 4.8/5 (47 download)

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Book Synopsis Flow Cytometry in Neoplastic Hematology by : Wojciech Gorczyca

Download or read book Flow Cytometry in Neoplastic Hematology written by Wojciech Gorczyca and published by CRC Press. This book was released on 2010-02-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

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Author :
Publisher : Cambridge University Press
ISBN 13 : 1107503833
Total Pages : 247 pages
Book Rating : 4.1/5 (75 download)

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Book Synopsis Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by : Anna Porwit

Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.