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Plant Microtechniques And Protocols
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Book Synopsis Plant Microtechniques and Protocols by : Edward Chee Tak Yeung
Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by Springer. This book was released on 2015-09-19 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Book Synopsis Plant Microtechniques and Protocols by : Edward Chee Tak Yeung
Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach. The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time. This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.
Book Synopsis Plant Microtechnique and Microscopy by : Steven E. Ruzin
Download or read book Plant Microtechnique and Microscopy written by Steven E. Ruzin and published by Oxford University Press, USA. This book was released on 1999 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.
Author :Víctor M. Loyola-Vargas Publisher :Springer Science & Business Media ISBN 13 :1592599591 Total Pages :389 pages Book Rating :4.5/5 (925 download)
Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas
Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
Book Synopsis Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region by : Duong Tan Nhut
Download or read book Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region written by Duong Tan Nhut and published by Springer Nature. This book was released on 2022-04-05 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.
Book Synopsis The Plant Cell Wall by : Zoë A. Popper
Download or read book The Plant Cell Wall written by Zoë A. Popper and published by Humana. This book was released on 2020-07-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field.
Book Synopsis Protocols for Macroalgae Research by : Bénédicte Charrier
Download or read book Protocols for Macroalgae Research written by Bénédicte Charrier and published by CRC Press. This book was released on 2018-04-17 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.
Book Synopsis Biological Microtechnique by : Jeremy Sanderson
Download or read book Biological Microtechnique written by Jeremy Sanderson and published by Garland Science. This book was released on 2020-08-13 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: A completely new practical guide to both new and classical methods of slide-making which is easy-to-read and easy-to-understand. Biological Microtechnique contains a wealth of practical detail which will provide a firm grounding in preparative methods for light microscopy.
Download or read book Plant Cell Biology written by and published by Academic Press. This book was released on 2020-08-31 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis. Provide in-depth procedures and application notes from selected experts who developed the methods Each chapter will include figures and movies as appropriate to explain complex techniques Chapters will include caveats of techniques and future prospects
Book Synopsis Plant-Pathogen Interactions by : Nora A. Foroud
Download or read book Plant-Pathogen Interactions written by Nora A. Foroud and published by Springer Nature. This book was released on 2023-05-30 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed collection focuses on current methodologies for studying various crop diseases. The book explores phytopathogen detection, host-pathogen interactions at the earliest stages of colonization, RNA-seq analyses, as well as host responses downstream of, or coincidental to, gene expression and a newly established protocol for gene editing of Medicago sativa. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant-Pathogen Interactions serves as an ideal guide for researchers working in the vital area of plant diseases.
Book Synopsis Plants, Politics and Empire in Ancient Rome by : Annalisa Marzano
Download or read book Plants, Politics and Empire in Ancient Rome written by Annalisa Marzano and published by Cambridge University Press. This book was released on 2022-10-13 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book investigates the cultural and political dimension of Roman arboriculture and the associated movement of plants from one corner of the empire to the other. It uses the convergent perspectives offered by textual and archaeological sources to sketch a picture of large-scale arboriculture as a phenomenon primarily driven by elite activity and imperialism. Arboriculture had a clear cultural role in the Roman world: it was used to construct the public persona of many elite Romans, with the introduction of new plants from far away regions or the development of new cultivars contributing to the elite competitive display. Exotic plants from conquered regions were also displayed as trophies in military triumphs, making plants an element of the language of imperialism. Annalisa Marzano argues that the Augustan era was a key moment for the development of arboriculture and identifies colonists and soldiers as important agents contributing to plant dispersal and diversity.
Book Synopsis Mountainous Forest Ecosystems: Challenges and Management Implications by : Shreekar Pant
Download or read book Mountainous Forest Ecosystems: Challenges and Management Implications written by Shreekar Pant and published by Frontiers Media SA. This book was released on 2023-11-13 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Frontiers in Phytolith Research by : Martin John Hodson
Download or read book Frontiers in Phytolith Research written by Martin John Hodson and published by Frontiers Media SA. This book was released on 2020-06-25 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols by : Yung-I Lee
Download or read book Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols written by Yung-I Lee and published by Humana Press. This book was released on 2018-05-27 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Book Synopsis Progress in Adhesion and Adhesives, Volume 4 by : K. L. Mittal
Download or read book Progress in Adhesion and Adhesives, Volume 4 written by K. L. Mittal and published by John Wiley & Sons. This book was released on 2019-07-11 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: A solid collection of interdisciplinary review articles on the latest developments in adhesion science and adhesives technology With the ever-increasing amount of research being published, it is a Herculean task to be fully conversant with the latest research developments in any field, and the arena of adhesion and adhesives is no exception. Thus, topical review articles provide an alternate and very efficient way to stay abreast of the state-of-the-art in many subjects representing the field of adhesion science and adhesives. Based on the success of the preceding volumes in this series "Progress in Adhesion and Adhesives"), the present volume comprises 9 review articles published in Volume 6 (2018) of Reviews of Adhesion and Adhesives. The subject of these reviews fall into the following general areas: 1. Adhesion to wood and wood bonds 2. Adhesive joints 3. Adhesion in microelectronic packaging 4. Surface modification 5. Contact angle, wettability and surface free energy. The topics covered include: Adhesion phenomena in microelectronic packaging; adhesives for wood and lignocellulosic materials; adhesion to wood and lignocellulosic materials; adhesively bonded lap joints having bi-adhesive and modulus-graded bondlines; adhesion between compounded elastomers; applications of contact angle measurements in pharmaceuticals and foods; oxygen or ammonia plasma treatment of polyolefin surfaces; surface free energy determination of powders and particles; wood bonds; and dispersion adhesion forces between macroscopic objects.
Book Synopsis Techniques for Work with Plant and Soil Nematodes by : Roland N. Perry
Download or read book Techniques for Work with Plant and Soil Nematodes written by Roland N. Perry and published by CABI. This book was released on 2020-11-26 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included.
Book Synopsis Abu Tbeirah Excavations I. Area 1 by : Licia Romano
Download or read book Abu Tbeirah Excavations I. Area 1 written by Licia Romano and published by Sapienza Università Editrice. This book was released on 2019-06-07 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the results of the archaeological activities and specialistic studies carried out at the site of Abu Tbeirah (Nasiriyah, Province of Dhi Qar, southern Iraq) by the Iraqi-Italian joint mission of the Iraqi State Board of Antiquities and Heritage and of Sapienza, led by F. D’Agostino and L. Romano (Dipartimento – Istituto Italiano di Studi Orientali). In the volume the accomplishments of the first seven campaigns (2011-2016) are introduced together with an assessment of the palaeo-environment and landscape surrounding the site. After an introduction to the reasons that led to start the archaeological activities in Abu Tbeirah, written by HE Dr A. Al-Hamdani, Minister of Culture of the Republic of Iraq, the diggings in the south-eastern Area 1 are presented (the cemetery and the other activities identified immediately under the top-soil and the last phase of Building A). A preliminary assessment on the Early-Dynastic III/Akkadian Transition pottery horizon (2450-2150 BC) is presented as well. At the same time, the multifaceted analyses and studies, carried out on Abu Tbeirah’s site and findings, are included in the volume.
