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Author : John Tane Christeller
Publisher :
ISBN 13 :
Total Pages : 486 pages
Book Rating : 4.3/5 (129 download)
Download or read book Phosphoglycolate Phosphatase written by John Tane Christeller and published by . This book was released on 1974 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mary Joyce Abbate
Publisher :
ISBN 13 :
Total Pages : 252 pages
Book Rating : 4.3/5 (129 download)
Download or read book Variations in 3-phosphoglycerate Phosphatase and Phosphoglycolate Phosphatase Activities with Physiological Changes written by Mary Joyce Abbate and published by . This book was released on 1971 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Daria Marie Engelmann
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (137 download)
Download or read book Regulation of Mammalian Phosphoglycolate Phosphatase written by Daria Marie Engelmann and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Daria Marie Engelmann
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (137 download)
Download or read book Regulation of Mammalian Phosphoglycolate Phosphatase written by Daria Marie Engelmann and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Isreal Moreno
Publisher :
ISBN 13 :
Total Pages : 150 pages
Book Rating : 4.:/5 (959 download)
Download or read book Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus Aureus written by Isreal Moreno and published by . This book was released on 2016 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Staphylococcus aureus is a major cause of hospital-acquired infections. The multi-drug resistant nature of certain S. aureus strains makes the discovery of new drug targets for S. aureus vital. A newly discovered virulence factor from S. aureus was described as an ortholog of NagD from E. coli, a member of the nitrophenyl phosphatase family of the HAD (Haloacid Dehalogenase) superfamily. This thesis will show that this virulence factor is not an ortholog of NagD UMPase from E. coli, but rather a phosphoglycolate phosphatase (PGPase). If phosphoglycolate accumulates in the cell, it will inhibit the glycolytic enzyme triose phosphate isomerase (TPI). In S. aureus, TPI also serves as an adhesion protein that can bind to host cells; phosphoglycolate would interfere with this adhesion process and thus make it harder for S. aureus to infect host cells. Thus, this S. aureus PGPase may act as a virulence factor by degrading the TPI inhibitor phosphoglycolate. We cloned the gene, expressed and purified the protein, and determined and characterized its activity. We have subcloned this PGPase into a His·Tag vector, purified the protein using nickel affinity and size exclusion chromatography, and characterized enzymatic activity, optimal conditions (substrate, pH, and metal usage), and kinetics."--Abstract.
Author : Gabriela Segerer
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (11 download)
Download or read book Characterization of Cell Biological and Physiological Functions of the Phosphoglycolate Phosphatase AUM written by Gabriela Segerer and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Regina A. Puts
Publisher :
ISBN 13 :
Total Pages : 120 pages
Book Rating : 4.:/5 (233 download)
Download or read book PHO13 Phosphoglycolate Phosphatase from Saccharomyces Cerevisiae (baker's Yeast), a Member of the P-nitrophenylphosphatase Family of the Haloalkanoic Acid Dehalogenase (HAD) Superfamily written by Regina A. Puts and published by . This book was released on 2010 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The function of phosphoglycolate phosphatase (PGPase) in photosynthetic organisms such as green algae (Chlamydomonas reinhardti) is to recycle the 2-phosphoglycolate that is formed as a by-product of the Calvin cycle. The role of PGPase in nonphotosynthetic organisms is less understood, but one potential role that has been suggested is to remove the 2-phosphoglycolate that is formed as a result of DNA repair. This may be the biological role of PHO13 PGPase in Saccharomyces cerevisiae as well. PHO13 PGPase from S. cerevisiae is a member of the PGPase subfamily within the p-nitrophenylphosphatase (p-NPPase) family, which is within the Haloalkanoic Acid Dehalogenase (HAD) superfamily. PHO13 has been subcloned into pET19b to create PHO13(His·Tag). PHO13(HT) was overexpressed and found to be soluble. It purified well by Ni2+-NTA affinity chromatography followed by size-exclusion chromatography. Its expression, solubility, and activity appeared comparable to the native PHO13, but PHO13(HT) was easier to purify due to the histidine tag. Both enzymes had comparable specific activities for both p-nitrophenylphosphate (p-NPP) and 2-phosphoglycolate (PG), pH optima around pH 8.0, optimal activity with >/= 7 mM Mg2+, activity with Co2+ and Mn2+, negligible activity in the presence of Zn2+, and no activity for Ca2+. PHO13(HT) is now ready for x-ray crystal structure determination with our collaborator, Joseph Wedekind, at the University of Rochester."--Abstract.
Author : Dietmar Schomburg
Publisher : Springer Science & Business Media
ISBN 13 : 9783540439837
Total Pages : 528 pages
Book Rating : 4.4/5 (398 download)
Download or read book Class 3.1 Hydrolases V written by Dietmar Schomburg and published by Springer Science & Business Media. This book was released on 2002-11-13 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized – and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.
Author : William Duncan Patterson Stewart
Publisher : Univ of California Press
ISBN 13 : 9780520024106
Total Pages : 1020 pages
Book Rating : 4.0/5 (241 download)
Download or read book Algal Physiology and Biochemistry written by William Duncan Patterson Stewart and published by Univ of California Press. This book was released on 1974 with total page 1020 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : M. Gibbs
Publisher : Springer Science & Business Media
ISBN 13 : 3642672426
Total Pages : 597 pages
Book Rating : 4.6/5 (426 download)
Download or read book Photosynthesis II written by M. Gibbs and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: M. GIBBS and E. LATZKO In the preface to his Experiments upon Vegetables, INGEN-Housz wrote in 1779: "The discovery of Dr. PRIESTLEY that plants have a power of correcting bad air . . . shows . . . that the air, spoiled and rendered noxious to animals by their breath ing in it, serves to plants as a kind of nourishment. " INGEN-Housz then described his own experiments in which he established that plants absorb this "nourishment" more actively in brighter sunlight. By the turn of the eighteenth century, the "nourishment" was recognized to be CO . Photosynthetic CO2 assimilation, the 2 major subject of this encyclopedia volume, had been discovered. How plants assimilate the CO was a question several successive generations 2 of investigators were unable to answer; scientific endeavor is not a discipline in which it is easy to "put the cart before the horse". The horse, in this case, was the acquisition of radioactive isotopes of carbon, especially 14c. The cart which followed contained the Calvin cycle, formulated by CALVIN, BENSON and BASSHAM in the early 1950's after (a) their detection of glycerate-3-P as the first stable product of CO fixation, (b) their discovery, and that by HORECKER 2 and RACKER, of the COz-fixing enzyme RuBP carboxylase, and (c) the reports by GIBBS and by ARNON of an enzyme (NADP-linked GAP dehydrogenase) capable of using the reducing power made available from sunlight (via photo synthetic electron transport) to reduce the glycerate-3-P to the level of sugars.
Author :
Publisher : Academic Press
ISBN 13 : 0080984215
Total Pages : 433 pages
Book Rating : 4.0/5 (89 download)
Download or read book Enzymes of Primary Metabolism written by and published by Academic Press. This book was released on 2012-12-02 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: The series Methods in Plant Biochemistry provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. The historical background and current, most useful methods of analysis are described. Detailed discussions of the protocols and suitability of each technique are included. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature.**Conceived as a practical comparison to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source.
Author : Gerry Edwards
Publisher : Univ of California Press
ISBN 13 :
Total Pages : 558 pages
Book Rating : 4./5 ( download)
Download or read book C, C written by Gerry Edwards and published by Univ of California Press. This book was released on 1983 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Bernard L. Horecker
Publisher : Elsevier
ISBN 13 : 148321706X
Total Pages : 243 pages
Book Rating : 4.4/5 (832 download)
Download or read book Current Topics in Cellular Regulation written by Bernard L. Horecker and published by Elsevier. This book was released on 2014-06-28 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Cellular Regulation, Volume 7 presents the fundamental mechanisms involved in the regulation of diverse cellular activities, including cellular differentiation, intermediary metabolism, and the transfer of genetic information. This book provides information pertinent to the various aspects of cellular regulation. Organized into six chapters, this volume begins with an overview of the activity of RuDP carboxylase in plant photosynthesis that appears to be controlled by the relative concentrations of F6P and FDP. This text then examines the glycolate pathway of metabolism in plants and algae. Other chapters consider the sequence of events as well as the mechanisms of regulation that are involved in the formation of the fibrin clot. This book discusses as well the biology of spermatogenesis. The final chapter deals with the mechanism of enzyme synthesis and its regulation at the gene level. This book is a valuable resource for biochemists, biologists, and research workers.
Author : Dietmar Schomburg
Publisher : Springer Science & Business Media
ISBN 13 : 3642764630
Total Pages : 836 pages
Book Rating : 4.6/5 (427 download)
Download or read book Enzyme Handbook written by Dietmar Schomburg and published by Springer Science & Business Media. This book was released on 2013-12-11 with total page 836 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are applied in organic synthesis and in analytical chemistry, in industrial production processes of pharmaceuticals and in food processing. Finding a suitable enzyme for a desired transformation or with a de- fined specificity is not always an easy task. More than 3000 enzymes are well described to date. The Enzyme Handbook provides all the information for selecting the proper enzyme to perform defined transformations in a given environment. The Enzyme Handbook devotes a variable number of pages for each enzyme, depending on the amount of information available with the EC number as ordering criterion within a volume. Revised data sheets can be released for individual enzymes and newly characterized enzymes and they can easily be sorted into the binders at the appropriate place. Each data sheet is divided into 7 sections: - Nomenclature (EC number, Systematic name, Recommended name, Synonyms, CAS Reg. No.). - Reaction and specificity (Catalysed reaction, Reaction type, Natural substrates, Substrate spectrum, Product spectrum, Inhibitors, Cofactors/prosthetic groups, Metal compounds/ salts, Turnover number, Specific activity, KM-value, pH-optimum, pH-range, Temperature optimum, Temperature range). - Enzyme structure (Molecular weight, Subunits, Glyco-/Lipoprotein). - Isolation/Preparation (Source organism, Source tissue, Localisation in source, Purification, Crystallization, Cloned, Renatured). - Stability (pH, Temperature, Oxidation, Organic solvent, General stability information, Storage). - Cross-References (to Structure Data Banks). - Literature references.
Author : Michael A. Sirover
Publisher : Academic Press
ISBN 13 : 0128098988
Total Pages : 326 pages
Book Rating : 4.1/5 (28 download)
Download or read book Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) written by Michael A. Sirover and published by Academic Press. This book was released on 2017-05-22 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH): The Quintessential Moonlighting Protein in Normal Cell Function and in Human Disease examines the biochemical protein interactions of the multi-dimensional protein GAPDH, further considering the regulatory mechanisms through which cells control their functional diversity. This protein’s diverse activities range from nuclear tRNA export and the maintenance of genomic integrity, to cytoplasmic post-transcriptional control of gene expression and receptor mediated cell signaling, to membrane facilitation of iron metabolism, trafficking and fusion. This book will be of great interest to basic scientists, clinicians and students, including molecular and cell biologists, immunologists, pathologists and clinical researchers who are interested in the biochemistry of GAPDH in health and disease. Contextualizes how GAPDH is utilized by cells in vivo Provides detailed insight into GAPDH post-translational modifications, including functional diversity and its subcellular localization Includes forward-thinking exposition on tough topics, such as the exploration of how GAPDG performs functions, how it decides where it should be present and requisite structural requirements
Author : J. Biggins
Publisher : Springer Science & Business Media
ISBN 13 : 940170516X
Total Pages : 860 pages
Book Rating : 4.4/5 (17 download)
Download or read book Progress in Photosynthesis Research written by J. Biggins and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: These Proceedings comprise the majority of the scientific contributions that were presented at the VIIth International Congress on Photosynthesis. The Congress was held August 10-15 1986 in Providence, Rhode Island, USA on the campus of Brown University, and was the first in the series to be held on the North American continent. Despite the greater average travel distances involved the Congress was attended by over 1000 active participants of whom 25% were registered students. This was gratifying and indicated that photosynthesis will be weIl served by excellent young scientists in the future. As was the case for the VIth International Congress held in Brussels, articles for these Proceedings were delivered camera ready to expedite rapid publication. In editing the volumes it was interesting to re fleet on the impact that the recent advances in structure and molecular biology had in this Congress. It is clear that cognizance of structure and molecular genetics will be even more necessary in the design of experiments and the direction of future research.
Author :
Publisher :
ISBN 13 :
Total Pages : 1332 pages
Book Rating : 4.3/5 (243 download)
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1988 with total page 1332 pages. Available in PDF, EPUB and Kindle. Book excerpt:
