Pcr In Neuroscience

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PCR in Neuroscience

Author :
Publisher : Elsevier
ISBN 13 : 9780080536453
Total Pages : 455 pages
Book Rating : 4.5/5 (364 download)

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Book Synopsis PCR in Neuroscience by :

Download or read book PCR in Neuroscience written by and published by Elsevier. This book was released on 1995-04-13 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) Complete retrieval of novel expressed genes by PCR without screening a library Quantitation by PCR Mutagenesis by PCR PCR in AIDS research Simple and effective protocols for PCR on archival specimens

Methods in Neurosciences: PCR in neuroscience

Author : P. Michael Conn
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (289 download)

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Book Synopsis Methods in Neurosciences: PCR in neuroscience by : P. Michael Conn

Download or read book Methods in Neurosciences: PCR in neuroscience written by P. Michael Conn and published by . This book was released on 1989 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Author : John T. Corthell
Publisher : Academic Press
ISBN 13 : 0128015276
Total Pages : 125 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls by : John T. Corthell

Download or read book Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls written by John T. Corthell and published by Academic Press. This book was released on 2014-04-16 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Methods in Neurosciences

Author : P. Michael Conn
Publisher :
ISBN 13 : 9780121852672
Total Pages : pages
Book Rating : 4.8/5 (526 download)

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Book Synopsis Methods in Neurosciences by : P. Michael Conn

Download or read book Methods in Neurosciences written by P. Michael Conn and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic Science Methods for Clinical Researchers

Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 382 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

In Situ Hybridization in Neurobiology

Author : James H. Eberwine
Publisher :
ISBN 13 : 9780195075076
Total Pages : 230 pages
Book Rating : 4.0/5 (75 download)

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Book Synopsis In Situ Hybridization in Neurobiology by : James H. Eberwine

Download or read book In Situ Hybridization in Neurobiology written by James H. Eberwine and published by . This book was released on 1994 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.

Methods of Behavior Analysis in Neuroscience

Author : Jerry J. Buccafusco
Publisher : CRC Press
ISBN 13 : 1420041819
Total Pages : 341 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Methods of Behavior Analysis in Neuroscience by : Jerry J. Buccafusco

Download or read book Methods of Behavior Analysis in Neuroscience written by Jerry J. Buccafusco and published by CRC Press. This book was released on 2000-08-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using the most well-studied behavioral analyses of animal subjects to promote a better understanding of the effects of disease and the effects of new therapeutic treatments on human cognition, Methods of Behavior Analysis in Neuroscience provides a reference manual for molecular and cellular research scientists in both academia and the pharmaceutic

Cyclin Dependent Kinase 5 (Cdk5)

Author : Nancy Y. Ip
Publisher : Springer Science & Business Media
ISBN 13 : 0387788875
Total Pages : 326 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Cyclin Dependent Kinase 5 (Cdk5) by : Nancy Y. Ip

Download or read book Cyclin Dependent Kinase 5 (Cdk5) written by Nancy Y. Ip and published by Springer Science & Business Media. This book was released on 2009-02-28 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyclin Dependent Kinase 5 provides a comprehensive and up-to-date collection of reviews on the discovery, signaling mechanisms and functions of Cdk5, as well as the potential implication of Cdk5 in the treatment of neurodegenerative diseases. Since the identification of this unique member of the Cdk family, Cdk5 has emerged as one of the most important signal transduction mediators in the development, maintenance and fine-tuning of neuronal functions and networking. Further studies have revealed that Cdk5 is also associated with the regulation of neuronal survival during both developmental stages and in neurodegenerative diseases. These observations indicate that precise control of Cdk5 is essential for the regulation of neuronal survival. The pivotal role Cdk5 appears to play in both the regulation of neuronal survival and synaptic functions thus raises the interesting possibility that Cdk5 inhibitors may serve as therapeutic treatment for a number of neurodegenerative diseases.

Current Protocols in Neuroscience

Author : Jacqueline N. Crawley
Publisher : Current Protocols
ISBN 13 : 9780471163619
Total Pages : pages
Book Rating : 4.1/5 (636 download)

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Book Synopsis Current Protocols in Neuroscience by : Jacqueline N. Crawley

Download or read book Current Protocols in Neuroscience written by Jacqueline N. Crawley and published by Current Protocols. This book was released on 1997-06-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Protocols in Neuroscience (CPN) draws from techniques in molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience to meet the specific needs of researchers in the full range of disciplines that is involved in studying the brain, nervous system, and corresponding behaviors. The editorial board of CPN have assembled an outstanding range of methods to enable users to explore their fields in greater depth and branch into related areas. The one-volume, looseleaf manual features carefully edited techniques with authors' troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates, filed into the looseleaf, keep you and your laboratory current with the latest developments in this rapidly changing field. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Pharmacology, and Toxicology.

Current Laboratory Methods in Neuroscience Research

Author : Huangui Xiong
Publisher : Springer
ISBN 13 : 9781493952168
Total Pages : 0 pages
Book Rating : 4.9/5 (521 download)

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Book Synopsis Current Laboratory Methods in Neuroscience Research by : Huangui Xiong

Download or read book Current Laboratory Methods in Neuroscience Research written by Huangui Xiong and published by Springer. This book was released on 2016-08-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Real-time PCR

Author : M Dorak
Publisher : Garland Science
ISBN 13 : 1134183992
Total Pages : 484 pages
Book Rating : 4.1/5 (341 download)

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Book Synopsis Real-time PCR by : M Dorak

Download or read book Real-time PCR written by M Dorak and published by Garland Science. This book was released on 2007-01-24 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

Understanding PCR

Author : Sarah Maddocks
Publisher : Academic Press
ISBN 13 : 0128026979
Total Pages : 98 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Understanding PCR by : Sarah Maddocks

Download or read book Understanding PCR written by Sarah Maddocks and published by Academic Press. This book was released on 2016-10-27 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

Neuroimaging in Developmental Clinical Neuroscience

Author : Judith M. Rumsey
Publisher : Cambridge University Press
ISBN 13 : 1139476750
Total Pages : 473 pages
Book Rating : 4.1/5 (394 download)

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Book Synopsis Neuroimaging in Developmental Clinical Neuroscience by : Judith M. Rumsey

Download or read book Neuroimaging in Developmental Clinical Neuroscience written by Judith M. Rumsey and published by Cambridge University Press. This book was released on 2009-02-19 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroimaging offers tremendous opportunities for gaining insights into normative development and a wide array of developmental neuropsychiatric disorders. Focusing on ontogeny, this text covers basic processes involved in both healthy and atypical maturation, and also addresses the range of neuroimaging techniques most widely used for studying children. This book will enable you to understand normative structural and functional brain maturation and the mechanisms underlying basic developmental processes; become familiar with current knowledge and hypotheses concerning the neural bases of developmental neuropsychiatric disorders; and learn about neuroimaging techniques, including their unique strengths and limitations. Coverage includes normal developmental processes, atypical processing in developmental neuropsychiatric disorders, ethical issues, neuroimaging techniques and their integration with psychopharmacologic and molecular genetic research approaches, and future directions. This comprehensive volume is an essential resource for neurologists, neuropsychologists, psychiatrists, pediatricians, and radiologists concerned with normal development and developmental neuropsychiatric disorders.

Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 030916785X
Total Pages : 223 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research by : National Research Council

Download or read book Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research written by National Research Council and published by National Academies Press. This book was released on 2003-08-22 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of these animals, and guidance on adapting these guidelines to various situations without hindering the research process. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research offers a more in-depth treatment of concerns specific to these disciplines than any previous guide on animal care and use. It treats on such important subjects as: The important role that the researcher and veterinarian play in developing animal protocols. Methods for assessing and ensuring an animal's well-being. General animal-care elements as they apply to neuroscience and behavioral research, and common animal welfare challenges this research can pose. The use of professional judgment and careful interpretation of regulations and guidelines to develop performance standards ensuring animal well-being and high-quality research. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research treats the development and evaluation of animal-use protocols as a decision-making process, not just a decision. To this end, it presents the most current, in-depth information about the best practices for animal care and use, as they pertain to the intricacies of neuroscience and behavioral research.

Creating Modern Neuroscience: The Revolutionary 1950s

Author : Gordon M. Shepherd
Publisher : Oxford University Press
ISBN 13 : 0195391500
Total Pages : 337 pages
Book Rating : 4.1/5 (953 download)

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Book Synopsis Creating Modern Neuroscience: The Revolutionary 1950s by : Gordon M. Shepherd

Download or read book Creating Modern Neuroscience: The Revolutionary 1950s written by Gordon M. Shepherd and published by Oxford University Press. This book was released on 2010 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: For modern scientists, history often starts with last week's journals and is regarded as largely a quaint interest compared with the advances of today. However, this book makes the case that, measured by major advances, the greatest decade in the history of brain studies was mid-twentieth century, especially the 1950s. The first to focus on worldwide contributions in this period, the book ranges through dozens of astonishing discoveries at all levels of the brain, from DNA (Watson and Crick), through growth factors (Hamburger and Levi-Montalcini), excitability (Hodgkin and Huxley), synapses (Katz and Eccles), dopamine and Parkinson's (Carlsson), visual processing (Hartline and Kuffler), the cortical column (Mountcastle), reticular activating system (Morruzzi and Magoun) and REM sleep (Aserinsky), to stress (Selye), learning (Hebb) and memory (HM and Milner). The clinical fields are also covered, from Cushing and Penfield, psychosurgery and brain energy metabolism (Kety), to most of the major psychoactive drugs in use today (beginning with Delay and Deniker), and much more.The material has been the basis for a highly successful advanced undergraduate and graduate course at Yale, with the classic papers organized and accessible on the web. There is interest for a wide range of readers, academic, and lay because there is a focus on the creative process itself, on understanding how the combination of unique personalities, innovative hypotheses, and new methods led to the advances. Insight is given into this process through describing the struggles between male and female, student and mentor, academic and private sector, and the roles of chance and persistence. The book thus provides a new multidisciplinary understanding of the revolution that created the modern field of neuroscience and set the bar for judging current and future advances.

Cellular and Molecular Methods in Neuroscience Research

Author : Adalberto Merighi
Publisher : Springer Science & Business Media
ISBN 13 : 0387224602
Total Pages : 307 pages
Book Rating : 4.3/5 (872 download)

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Book Synopsis Cellular and Molecular Methods in Neuroscience Research by : Adalberto Merighi

Download or read book Cellular and Molecular Methods in Neuroscience Research written by Adalberto Merighi and published by Springer Science & Business Media. This book was released on 2007-12-14 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Handbook of Molecular-Genetic Techniques for Brain and Behavior Research

Author : Wim E. Crusio
Publisher : Elsevier
ISBN 13 : 9780080536866
Total Pages : 962 pages
Book Rating : 4.5/5 (368 download)

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Book Synopsis Handbook of Molecular-Genetic Techniques for Brain and Behavior Research by : Wim E. Crusio

Download or read book Handbook of Molecular-Genetic Techniques for Brain and Behavior Research written by Wim E. Crusio and published by Elsevier. This book was released on 1999-10-18 with total page 962 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book gives a broad overview of recombinant DNA techniques for the behavioral neuroscientist, with illustrative examples of applications. Species covered include rodents (mainly mice), Drosophila melanogaster, Caenorhabditis elegans and Danio rerio. Experimental techniques required to characterize the behavioral phenotypes of mutant animals is provided. Several aspects of novel molecular-genetic techniques are overviewed and possible research strategies are explained. The sections of the book start with general descriptions of techniques followed by illustrative examples. It is divided into six sections. Section 1, bioinformatics and genomics research. Section 2, top-down strategies, where the researcher starts with the phenotype and then analyzes the associated genes; bottom-up strategies, where the physiological chain leading to a phenotype is analyzed starting from the gene product. Section 3, transgenic approaches in rodents including overexpressing foreign genes and gene-targeting; systemic manipulation approaches directly targeting the central nervous system and methods used with invertebrates. Section 4, methods used to evaluate relevant behavioral phenotypes, including learning and aggression. Section 5, examples on molecular brain research in man. Section 6, ethical aspects of research in this field.