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Author : Per Åke Albertsson
Publisher :
ISBN 13 :
Total Pages : 244 pages
Book Rating : 4.:/5 (319 download)
Download or read book Partition of Cell Particles and Macromolecules written by Per Åke Albertsson and published by . This book was released on 1960 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Harry Walter
Publisher : Elsevier
ISBN 13 : 9780121821296
Total Pages : 768 pages
Book Rating : 4.8/5 (212 download)
Download or read book Aqueous Two-Phase Systems written by Harry Walter and published by Elsevier. This book was released on 1994-04-18 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Per Åke Albertsson
Publisher :
ISBN 13 :
Total Pages : 346 pages
Book Rating : 4.:/5 (4 download)
Download or read book Partition of Cell Particles and Macromolecules written by Per Åke Albertsson and published by . This book was released on 1971 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Kamalesh Sirkar
Publisher : Cambridge University Press
ISBN 13 : 0521895731
Total Pages : 923 pages
Book Rating : 4.5/5 (218 download)
Download or read book Separation of Molecules, Macromolecules and Particles written by Kamalesh Sirkar and published by Cambridge University Press. This book was released on 2014-01-16 with total page 923 pages. Available in PDF, EPUB and Kindle. Book excerpt: A modern separation process textbook written for advanced undergraduate and graduate level courses in chemical engineering.
Author : Munishwar N. Gupta
Publisher : Springer Science & Business Media
ISBN 13 : 9783764363055
Total Pages : 240 pages
Book Rating : 4.3/5 (63 download)
Download or read book Methods for Affinity-Based Separations of Enzymes and Proteins written by Munishwar N. Gupta and published by Springer Science & Business Media. This book was released on 2002-04-01 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Author : Harry Walter
Publisher : Elsevier
ISBN 13 : 0323142915
Total Pages : 731 pages
Book Rating : 4.3/5 (231 download)
Download or read book Partitioning In Aqueous Two – Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Author : Per-Ake Albertsson
Publisher : Wiley-Interscience
ISBN 13 : 9780471828204
Total Pages : 346 pages
Book Rating : 4.8/5 (282 download)
Download or read book Partition of Cell Particles and Macromolecules written by Per-Ake Albertsson and published by Wiley-Interscience. This book was released on 1986-03-28 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.
Author : Robert K. Scopes
Publisher : Springer Science & Business Media
ISBN 13 : 9780387940724
Total Pages : 408 pages
Book Rating : 4.9/5 (47 download)
Download or read book Protein Purification written by Robert K. Scopes and published by Springer Science & Business Media. This book was released on 1993-11-19 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
Author : Roza Maria Kamp
Publisher : Springer Science & Business Media
ISBN 13 : 3642592198
Total Pages : 311 pages
Book Rating : 4.6/5 (425 download)
Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.
Author : Thomas G. Pretlow
Publisher : Academic Press
ISBN 13 : 1483219410
Total Pages : 399 pages
Book Rating : 4.4/5 (832 download)
Download or read book Cell Separation written by Thomas G. Pretlow and published by Academic Press. This book was released on 2014-05-10 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including liver cells, epidermal Langerhans cells, isolation of oval cells, clonal analysis, and the purification of polymorphonuclear leukocytes. Organized into 17 chapters, this volume begins with an overview of the central role of the liver in the metabolism of the body. This text then provides the analysis of Langerhans cells that allow modulation of their function and provide approaches to the treatment of skin disease. Other chapters consider the biological significance of oval cells. This book discusses as well the elucidation of the mechanisms of cellular proliferation, function, and differentiation in living tissues. The final chapter deals with the important applications of cell culture that involve continuous cell lines. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.
Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
ISBN 13 : 1592590284
Total Pages : 442 pages
Book Rating : 4.5/5 (925 download)
Download or read book Aqueous Two-Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Author : Adam Baszkin
Publisher : CRC Press
ISBN 13 : 1482293226
Total Pages : 849 pages
Book Rating : 4.4/5 (822 download)
Download or read book Physical Chemistry of Biological Interfaces written by Adam Baszkin and published by CRC Press. This book was released on 1999-11-22 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the most important fundamental concepts of physicochemical interface science and a description of experimental techniques and applications of surface science in relation to biological systems. It explores artificial assemblies of lipids, proteins and polysaccharides that perform novel functions that living systems cannot duplicat
Author : Rob Phillips
Publisher : Garland Science
ISBN 13 : 1134111584
Total Pages : 1089 pages
Book Rating : 4.1/5 (341 download)
Download or read book Physical Biology of the Cell written by Rob Phillips and published by Garland Science. This book was released on 2012-10-29 with total page 1089 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Biology of the Cell is a textbook for a first course in physical biology or biophysics for undergraduate or graduate students. It maps the huge and complex landscape of cell and molecular biology from the distinct perspective of physical biology. As a key organizing principle, the proximity of topics is based on the physical concepts that
Author :
Publisher : Academic Press
ISBN 13 : 0080857310
Total Pages : 369 pages
Book Rating : 4.0/5 (88 download)
Download or read book Microcompartmentation and Phase Separation in Cytoplasm written by and published by Academic Press. This book was released on 1999-10-21 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.This volume provides an overview of major cytoplasmic properties and events which including cytoarchitecture and the physical properties of cytoplasm, molecular compartmentation and gradients, channeling, sorting, and trafficking. It also addresses physicochemical events, both measured and anticipated, which attend solutions under conditions prevailing in cytoplasm: molecular crowding. It summarizes the current state of knowledge in the field and considers questions such as how molecules in cytoplasm interact.
Author : That T. Ngo
Publisher : Springer Science & Business Media
ISBN 13 : 1489918728
Total Pages : 560 pages
Book Rating : 4.4/5 (899 download)
Download or read book Molecular Interactions in Bioseparations written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Author : Nikolai Lebovka
Publisher : CRC Press
ISBN 13 : 143984593X
Total Pages : 572 pages
Book Rating : 4.4/5 (398 download)
Download or read book Enhancing Extraction Processes in the Food Industry written by Nikolai Lebovka and published by CRC Press. This book was released on 2011-09-26 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extraction is an important operation in food engineering, enabling the recovery of valuable soluble components from raw materials. With increasing energy costs and environmental concerns, industry specialists are looking for improved techniques requiring less solvents and energy consumption. Enhancing Extraction Processes in the Food Industry is a comprehensive resource providing clear descriptions of the latest extraction methods and instruments used in food laboratories. The book begins with an overview of solvent extraction technology. It examines pulsed electric fields and their effect on food engineering, and the potential and limitations of microwave-assisted extraction. It explores diffusion processes and reviews what is known about electrical discharge processes in the extraction of biocompounds. Next, the book summarizes current knowledge on conventional and innovative techniques for the intensification of extractions from food and natural products, focusing on environmental impacts. It reviews recent developments in supercritical CO2 extraction of food and food products, describes the pressurized hot water extraction (PHWE) process, and examines future trends for PHWE. The book also examines essential oil extraction, and the tools and techniques of high pressure-assisted extraction. The authors demonstrate its application using litchi and longan fruits as examples. The final chapters focus on extrusion-assisted extraction, gas-assisted mechanical expression, mechanochemically assisted extraction, reverse micellar extraction, and aqueous two-phase extraction. The book concludes with a chapter on the treatment of soybeans through enzyme-assisted aqueous processing, examining the economics involved as well as the development of the process. A solid review of modern approaches that enhance extraction processes, this volume is destined to pave the way for future research and development in the field.
