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Novel Detection Schemes And High Resolution Separations In Microcolumn Liquid Separations
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Book Synopsis Novel Detection Schemes and High Resolution Separations in Microcolumn Liquid Separations by : James N. Alexander
Download or read book Novel Detection Schemes and High Resolution Separations in Microcolumn Liquid Separations written by James N. Alexander and published by Uppsala Universitet. This book was released on 1999 with total page 41 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microcolumn Separations by : M.V. Novotny
Download or read book Microcolumn Separations written by M.V. Novotny and published by Elsevier. This book was released on 2011-09-22 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited by two of the pioneers of microcolumn chromatography and written by recognized experts in the field, this book summarizes advances in microcolumn liquid chromatography, capillary supercritical fluid chromatography and microelectrophoresis. Its unique combination of expert knowledge from leading laboratories in the USA, Japan and Switzerland, results in a particularly in-depth and comprehensive coverage of the various aspects of microcolumn separation methods.
Book Synopsis Microcolumn Separations by : Miloš V. Novotný
Download or read book Microcolumn Separations written by Miloš V. Novotný and published by . This book was released on 1985 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Column studies. Miniaturized systems. Spectroscopic detection. Electrochemical detection.
Book Synopsis High Resolution Separations by Liquid Chromatography and Capillary Electrophoresis by : Michelle M. Bushey
Download or read book High Resolution Separations by Liquid Chromatography and Capillary Electrophoresis written by Michelle M. Bushey and published by . This book was released on 1990 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advancements in Detection for Microcolumn Separations by : Keith Edward Fadgen
Download or read book Advancements in Detection for Microcolumn Separations written by Keith Edward Fadgen and published by . This book was released on 2001 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Hyphenated and Alternative Methods of Detection in Chromatography by : R. Andrew Shalliker
Download or read book Hyphenated and Alternative Methods of Detection in Chromatography written by R. Andrew Shalliker and published by CRC Press. This book was released on 2011-12-14 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: Widely employed for separating and detecting chemicals in solution, separation techniques are most often applied in tandem, subsequently referred to as hyphenated methods. Hyphenated and Alternative Methods of Detection in Chromatography details the development and application of mass spectral detection techniques coupled with gas phase and liquid
Book Synopsis A Century of Separation Science by : Haleem J. Issaq
Download or read book A Century of Separation Science written by Haleem J. Issaq and published by CRC Press. This book was released on 2001-12-19 with total page 782 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Century of Separation Science presents an extensive overview of the critical developments in separation science since 1900, covering recent advances in chromatography, electrophoresis, field-flow fractionation, countercurrent chromatography, and supercritical fluid chromatography for high-speed and high-throughput analysis.
Book Synopsis Instrumentation for Microcolumn Separations by : Murray Hackett
Download or read book Instrumentation for Microcolumn Separations written by Murray Hackett and published by . This book was released on 1991 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis DEVELOPMENT OF NOVEL METHODS FOR THE RAPID SEPARATION OF BIOMOLECULES by : Manasa Mamunooru
Download or read book DEVELOPMENT OF NOVEL METHODS FOR THE RAPID SEPARATION OF BIOMOLECULES written by Manasa Mamunooru and published by . This book was released on 2013 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful methods for the separation of biomolecules like amino acids, proteins, peptides, and DNA have been developed previously using HPLC, GC, GC-MS, and CE. Recently CE has become a routine laboratory technique in the analysis of biological molecules. Even though high-resolution separations with small sample volumes is the main advantage, CE is limited by lower sensitivity detection of analytes when universal detectors like UV absorption or refractive index detectors are used. Therefore, sensitivity enhancement can be obtained by either using different detection schemes or electrophoretically based pre- or on-line concentration methods. These can be grouped into two categories. The first category includes IEF, CGF or TGF where sensitivity is achieved through equilibrium electrofocusing. In these methods, electrophoresis and bulk solution is combined in the capillary or separation column to form a null velocity point, a point at which the net velocity of the analyte is zero. Using these methods 10-10,000 fold sensitivity enhancement is achieved. The second category uses velocity gradients but not the nul velocity for the enrichment of samples. These methods include FASS, LVSS, NSM, etc., which are applied for the analysis of small molecules, and 10-10,000 fold sensitivity enhancement is reported by using these methods. In this work, first GEITP an on-line preconcentration technique is applied for the detection of amino acids (using Trp and Tyr as model analytes). This work also established the effects of different parameters on enrichment. The parameters studied include effect of current flow acceleration across capillary inner diameter, the effect of leading electrolyte (LE) concentration on current density, and the effect of applied electric fields on the current density. To explore the application of GEITP in biological fluids, optimized parameters were developed for the detection and separation of Trp and Tyr in artificial cerebrospinal fluid (aCSF). Next, GEITP was applied for enrichment and separation of physiologically relevant concentrations of chromophore-derivatized Asp and Glu in high conductivity samples like artificial cerebrospinal fluid (aCSF). It was concluded from this work that the major factors which influence the enrichment is the ratio of current density to sample conductivity. Finally, GEITP is applied as a prior step before CZE to increase the resolution between analytes without using ampholyte mixtures. In this method GEITP was combined to CZE to achieve resolution adjustment between amino acids mixture using low pressure hydrodynamic flow during CZE without changing the separation column, field strength, or electrolyte system. In this work, a rapid CE method for extraction and analysis of amino acids in planarians, labeled with 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), was developed. This method was applied to detect the changes in the levels of amino acids when planarians were fed and starved. This method can be applied to study pharmacological effects in planaria, as it can monitor different amino acid levels with respect to feeding. Finally, ssDNA photoproducts of different lengths (11-mer and 63-mer) were separated using two different matrices, a traditional C18 and a PV/DBS (PLRP-S) matrix. A faster separation (within ~ 10 mins) was achieved for a 11-mer by the PLRP-S column. A separation was achieved in the PLRP-S column for the 63-mer while there was no separation in C18 column. Baseline resolution was not achieved. Therefore, C18 can best be used for small length DNA while PLRP-S can be applied for longer length DNA, as it is more hydrophobic than C18 column. Parameters can still be optimized for a baseline separation.
Book Synopsis Carbohydrate Analysis by Modern Liquid Phase Separation Techniques by : Ziad El Rassi
Download or read book Carbohydrate Analysis by Modern Liquid Phase Separation Techniques written by Ziad El Rassi and published by Elsevier. This book was released on 2021-08-15 with total page 956 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Book Synopsis Applications of Conductivity Detection in Microcolumn Separations by : Stephen E. Johnston
Download or read book Applications of Conductivity Detection in Microcolumn Separations written by Stephen E. Johnston and published by . This book was released on 2005 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Novel Approaches for the Chromatographic and Electrophoretic Separation of Molecules by : Amanda R. Meyer
Download or read book Novel Approaches for the Chromatographic and Electrophoretic Separation of Molecules written by Amanda R. Meyer and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: High-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are two well-established analytical separation techniques that are continuously being adapted for performing distinctive separations and analyses of multitudes of complex and/or unique samples. Since their introduction, these techniques have been pivotal in the discovery, analysis, and understanding of a variety of samples and still prove to be key analytical tools for biological investigation. Using these techniques, one can obtain a wide-range of valuable sample information from the hydrophobicity and molecular weights to size and charge distributions. Furthermore, these techniques allow for sample analysis, purification, and collection for additional sample analysis, such as mass spectrometry analysis. My doctoral dissertation encompasses the full scope of these two techniques and novel approaches for the investigation of distinct, relevant samples. Described herein is the fabrication of glass microfluidic devices used for CE and their diversity for numerous investigations. Chapter 2 shows that the resolution of the photomasks used in microchip fabrication does not alter the separation efficiency of the devices, as the separations remain diffusion-limited. Using an in-house built capillary electrophoresis system, wheat proteins were separated more than 25% faster than previously reported in literature, and the electropherograms used for sample varietal identification. The fabrication of a robust, portable CE system capable of performing biological analysis in microgravity and hypergravity environments is also discussed. The need for and features necessary to achieve a reliable, robust, automated system is further described in Chapter 4. Isolation and analysis of the pea aphid (Acyrthosiphon pisum) salivary secretions was completed for the first time using HPLC. By altering the aphid environment and the sample treatment parameters, sample concentrations were increased above the limit of detection. Coupled with mass spectrometry, identification of pea aphid salivary proteins such as exopeptidase, angiotensin converting enzyme, and Buchnera proteins has been achieved. Finally, a simplified contact conductivity detection system for the detection of jurkat cells was developed that surpasses current, complex optical systems. The experiments described in this dissertation demonstrate novel approaches for the preparation, separation, analysis, and identification of a wide variety of common, and uncommon, samples.
Book Synopsis Separation Techniques Applied to Omics Sciences by : Ana Valéria Colnaghi Simionato
Download or read book Separation Techniques Applied to Omics Sciences written by Ana Valéria Colnaghi Simionato and published by Springer Nature. This book was released on 2021-10-09 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.
Book Synopsis Detection Aspects of Microcolumn Liquid Chromatography by : Stephen C. Beale
Download or read book Detection Aspects of Microcolumn Liquid Chromatography written by Stephen C. Beale and published by . This book was released on 1989 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Separation, Purification and Identification by : Lesley E Smart
Download or read book Separation, Purification and Identification written by Lesley E Smart and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book looks at the common techniques used to prepare, purify and identify chemicals. Topics including distillation, recrystallisation, chromatography, elemental analysis, atomic absorption spectroscopy and mass spectrometry are discussed, and are illustrated on video on the accompanying CD-ROMs. Infrared and nuclear magnetic resonance spectroscopy are covered entirely through multi-media, with animations and virtual experiments. The reader is provided with examples for interpretation, and can draw in the structures using the software provided. There is also a set of interactive self-assessment questions. In all, the multi-media software suite comprises more than twelve hours of material. Separation, Purification and Identification concludes with a Case Study on Forensic Science, in which illustrations of criminal cases where spectroscopic techniques provided evidence are given. The Molecular World series provides an integrated introduction to all branches of chemistry for both students wishing to specialise and those wishing to gain a broad understanding of chemistry and its relevance to the everyday world and to other areas of science. The books, with their Case Studies and accompanying multi-media interactive CD-ROMs, will also provide valuable resource material for teachers and lecturers. (The CD-ROMs are designed for use on a PC running Windows 95, 98, ME or 2000.)
Book Synopsis Measurement of Quality in Microcolumn Separations by : Sheldon R. Sumpter
Download or read book Measurement of Quality in Microcolumn Separations written by Sheldon R. Sumpter and published by . This book was released on 1992 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Trace Analysis with Microcolumn Liquid Chromatography by : Milos Krejci
Download or read book Trace Analysis with Microcolumn Liquid Chromatography written by Milos Krejci and published by CRC Press. This book was released on 1992-04-15 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
