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Neuropeptide Protocols Methods In Molecular Biology
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Book Synopsis Neuropeptide Protocols. Methods in Molecular Biology by : G. Brent Irvine
Download or read book Neuropeptide Protocols. Methods in Molecular Biology written by G. Brent Irvine and published by . This book was released on 1997 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.
Author :Ambikaipakan Balasubramaniam Publisher :Springer Science & Business Media ISBN 13 :159259042X Total Pages :244 pages Book Rating :4.5/5 (925 download)
Book Synopsis Neuropeptide Y Protocols by : Ambikaipakan Balasubramaniam
Download or read book Neuropeptide Y Protocols written by Ambikaipakan Balasubramaniam and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: The observation that neuropeptide Y (NPY) is the most abundant peptide present in the mammalian nervous system and the finding that it elicits the most powerful orexigenic signal have led to active investigations of the properties of the NPY family of hormones, including peptide YY (PYY) and pancreatic polypeptide (PP). Nearly two decades of research have led to the identification of several NPY receptor subtypes and the development of useful receptor selective ligands. Moreover, these investigations have imp- cated NPY in the pathophysiology of a number of diseases, including feeding disorders, seizures, memory loss, anxiety, depression, and heart failure. Vigorous efforts are therefore continuing, not only to understand the bioche- cal aspects of NPY actions, but also toward developing NPY-based treatments for a variety of disorders. To facilitate these efforts, it was decided to produce the first handbook on NPY research techniques as part of the Methods in Molecular Biology Series. In compiling Neuropeptide Y Protocols, I have gathered contributions on techniques considered critical for the advancement of the NPY field from experts in various disciplines. Each chapter starts with a brief introduction, with Materials and Methods sections following. The latter sections are presented in an easy to follow step-by-step format. The last section of the chapter, Notes, highlights pitfalls and the maneuvers employed to overcome them. This information, not usually disseminated in standard research pub- cations, may prove extremely useful for investigators employing these te- niques in NYP research.
Book Synopsis Neuropeptides by : Adalberto Merighi
Download or read book Neuropeptides written by Adalberto Merighi and published by Humana Press. This book was released on 2017-04-30 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy. Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field. The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.
Book Synopsis Neuropeptide Protocols by : G. Brent Irvine
Download or read book Neuropeptide Protocols written by G. Brent Irvine and published by Humana. This book was released on 2013-09-10 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: After 20 years of intensive effort, novel neuropeptides continue to be discovered, and the field of neuropeptide research is still expanding. As new analytical techniques become available, their applicability to the study of neu ropeptides brings fresh insights into the properties and functions of these ubiq uitous chemical messengers. Presented in this single volume, Neuropeptide Protocols, are 33 chapters covering these new techniques, together with more established methods. Each contributor is actively engaged in neuropeptide research and so brings to his or her description an awareness of the practical problems inher ent in the method, and provides sound advice on how to overcome them. The format conforms to the style of previous books in the Methods in Molecular Biology series. Each chapter provides an instruction to the technique, and item ized list of equipment and reagents, and a step-by-step set of instructions to enable practitioners to reproduce the method. The Notes section gives insights into pitfalls or critical stages, tips to overcome these obstacles, and sugges tions for extensions or modifications of the basic protocol. Neuropeptide Protocols is intended as a benchtop manual providing the entire gamut of techniques that form the essential tool kit of the practicing neuropeptide researcher. It will be useful for those new to the field, as well as for established workers who wish to try a new technique for the first time.
Book Synopsis Neuropeptide Y Protocols by : Ambikaipakan Balasubramaniam
Download or read book Neuropeptide Y Protocols written by Ambikaipakan Balasubramaniam and published by Humana Press. This book was released on 2000-07-07 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: The observation that neuropeptide Y (NPY) is the most abundant peptide present in the mammalian nervous system and the finding that it elicits the most powerful orexigenic signal have led to active investigations of the properties of the NPY family of hormones, including peptide YY (PYY) and pancreatic polypeptide (PP). Nearly two decades of research have led to the identification of several NPY receptor subtypes and the development of useful receptor selective ligands. Moreover, these investigations have imp- cated NPY in the pathophysiology of a number of diseases, including feeding disorders, seizures, memory loss, anxiety, depression, and heart failure. Vigorous efforts are therefore continuing, not only to understand the bioche- cal aspects of NPY actions, but also toward developing NPY-based treatments for a variety of disorders. To facilitate these efforts, it was decided to produce the first handbook on NPY research techniques as part of the Methods in Molecular Biology Series. In compiling Neuropeptide Y Protocols, I have gathered contributions on techniques considered critical for the advancement of the NPY field from experts in various disciplines. Each chapter starts with a brief introduction, with Materials and Methods sections following. The latter sections are presented in an easy to follow step-by-step format. The last section of the chapter, Notes, highlights pitfalls and the maneuvers employed to overcome them. This information, not usually disseminated in standard research pub- cations, may prove extremely useful for investigators employing these te- niques in NYP research.
Download or read book Peptidomics written by Mikhail Soloviev and published by Humana. This book was released on 2016-08-23 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.
Book Synopsis Peptidases and Neuropeptide Processing by :
Download or read book Peptidases and Neuropeptide Processing written by and published by Academic Press. This book was released on 1995-02-08 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Comprehensive protocols included for: Enzymes involved in the activation of bioactive peptidases and proteins Prohormone/neuropeptide processing pathways Enzymes involved in peptide metabolism Posttranslational processing enzymes
Book Synopsis Neuropeptide Techniques by : Illana Gozes
Download or read book Neuropeptide Techniques written by Illana Gozes and published by Springer Science & Business Media. This book was released on 2007-11-13 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neuropeptides exhibit multiple functions as hormones and regulators. As such, many techniques are required to understand the contribution and functions of neuropeptides in health and disease processes. Research in the neuropeptide field is aimed toward a better understanding of the physiology of the human body and toward the development of better diagnostics and therapeutics. This book will familiarize the audience with forefront techniques in the field.
Book Synopsis Neuropeptide Technology by : P. Michael Conn
Download or read book Neuropeptide Technology written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 6: Neuropeptide Technology: Synthesis, Assay, Purification, and Processing describes procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into four sections— chemical synthesis and biosynthesis; measurement of neuropeptides; purification and characterization; and neuropeptide degrading and processing enzymes. In these sections, this book specifically discusses the synthesis of peptide substrates for protein kinase C; synthesis of glycosyl neuropeptides; and ultrastructural localization of peptides. The measurement of neurokinin B by radioimmunoassay; purification and characterization of neuroendocrine peptides from rat brain; and preparation of glia maturation factor ß are also elaborated. This text likewise covers the assays for arginine/lysine carboxypeptidases and enzymes that metabolize atrial natriuretic peptide. This publication is beneficial to neuroscientists and students researching on the synthesis, assay, purification, and processing of neuropeptides.
Book Synopsis Neuropeptide Analogs, Conjugates, and Fragments by : P. Michael Conn
Download or read book Neuropeptide Analogs, Conjugates, and Fragments written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 13: Neuropeptide Analogs, Conjugates, and Fragments covers the synthesis and characterization of peptide analogs, conjugates, and fragments, their use as ligands for receptors, and their role in the development and use of antisera. The book discusses techniques such as novel synthetic approaches; biotinylation; purification and characterization; radioligand techniques and assay development; use of agonists and antagonists; distinguishing receptor subtypes; conjugation to carrier proteins; antiidiotypic antibody development; and radiolabeling. Neuroscientists, biochemists, neurochemists, and pharmacologists will find the book useful.
Book Synopsis Peptide Nucleic Acids by : Peter E. Nielsen
Download or read book Peptide Nucleic Acids written by Peter E. Nielsen and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .
Book Synopsis Therapeutic Peptides by : Andrew E. Nixon
Download or read book Therapeutic Peptides written by Andrew E. Nixon and published by . This book was released on 2014 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.
Book Synopsis Neuropeptide Technology by : P. Michael Conn
Download or read book Neuropeptide Technology written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 5: Neuropeptide Technology: Gene Expression and Neuropeptide Receptors discusses procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into two sections—neuropeptide gene expression and neuropeptide receptors. In these sections, this book specifically discusses the assays for peptide products of somatostatin gene expression; molecular assays for rat thyrotropin-releasing hormone gene; and structure, expression, and chromosomal localization of human gene. The gastrin-releasing peptide or mammalian bombesin; semiquantitative analysis of cellular somatostatin mRNA Levels; and atrial natriuretic factor receptor assays are also elaborated. This text likewise covers the receptors for opioid peptides in brain and autoradiography of somatostatin receptors in rat cerebellum. This publication is beneficial to neuroscientists and students intending to acquire knowledge of gene expression and neuropeptide receptors.
Download or read book Peptidomics written by Michael Schrader and published by Humana. This book was released on 2019-06-04 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls. Authoritative and practical, Peptidomics: Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics.
Book Synopsis The Protein Protocols Handbook by : John M. Walker
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 1996 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: -- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students
Download or read book Oxytocin written by Eryn L. Werry and published by Humana. This book was released on 2021-11-08 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book explores techniques for further elucidating the peripheral and central roles of oxytocin as well as techniques key to oxytocin receptor-related drug discovery. The first set of chapters explore this neuropeptide’s peripheral and central effects, such as regulation of myometrial contraction, induction of cardioprotective effects, and the facilitation of pro-social behaviors. The book then continues by delving into a comprehensive pharmacological characterization of oxytocin receptor ligands and ligands of other key receptors such as the vasopressin receptor family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oxytocin: Methods and Protocols is an ideal guide for researchers seeking to further our knowledge of the varied and power effects of oxytocin within the central nervous system.
Book Synopsis Peptide Self-Assembly by : Bradley L. Nilsson
Download or read book Peptide Self-Assembly written by Bradley L. Nilsson and published by Humana. This book was released on 2019-05-24 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of self-assembled peptide materials, and structural and mechanistic analyses of pathological amyloid systems that provide novel ways to assess function of the various possible aggregates as well to determine how the structure of these materials correlates to function/dysfunction in the biological context. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Peptide Self-Assembly: Methods and Protocols aims to capture modern methods that span the breadth of the exciting and expanding field of peptide self-assembly.
