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Monoclonal Hybridoma Antibodies
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Book Synopsis Monoclonal Antibody Production by : National Research Council
Download or read book Monoclonal Antibody Production written by National Research Council and published by National Academies Press. This book was released on 1999-05-06 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Book Synopsis Monoclonal Antibodies by : Roger H. Kennett
Download or read book Monoclonal Antibodies written by Roger H. Kennett and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid oma technology has been observing how the implications of the initial observa tions gradually spread first among the practitioners of immunology and immu nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.
Book Synopsis Monoclonal Antibodies by : Vincent Ossipow
Download or read book Monoclonal Antibodies written by Vincent Ossipow and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.
Book Synopsis Monoclonal Antibody Production Techniques and Applications by : Lawrence B. Schook
Download or read book Monoclonal Antibody Production Techniques and Applications written by Lawrence B. Schook and published by New York : Dekker. This book was released on 1987 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Practical Guide to Monoclonal Antibodies by : J. Eryl Liddell
Download or read book A Practical Guide to Monoclonal Antibodies written by J. Eryl Liddell and published by John Wiley & Sons. This book was released on 1991-08-26 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Book Synopsis Monoclonal Hybridoma Antibodies by : John G.R. Hurrell
Download or read book Monoclonal Hybridoma Antibodies written by John G.R. Hurrell and published by CRC Press. This book was released on 2018-05-04 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.
Book Synopsis Human Hybridomas and Monoclonal Antibodies by : Edgar Engleman
Download or read book Human Hybridomas and Monoclonal Antibodies written by Edgar Engleman and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.
Book Synopsis Methods for Production of Monoclonal Antibodies by : Geoffrey Josiah Letchworth
Download or read book Methods for Production of Monoclonal Antibodies written by Geoffrey Josiah Letchworth and published by . This book was released on 1984 with total page 58 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Hybridoma Technology in the Biosciences and Medicine by : Timothy Springer
Download or read book Hybridoma Technology in the Biosciences and Medicine written by Timothy Springer and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Monoclonal Antibodies in Biotechnology by : Kenneth C. McCullough
Download or read book Monoclonal Antibodies in Biotechnology written by Kenneth C. McCullough and published by Cambridge University Press. This book was released on 2009-03-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a complete description of the principles, methodologies and applications of monoclonal antibodies, one of the most exciting developments to occur in biotechnology in recent years, and a powerful technology for modern industry and science. The immune system and the role of the antibody are described and full details are given on how the hybridomas are formed, isolated, and maintained in culture such that the required antibody can be produced to a high degree of purity. The authors describe all the methodologies involved, all the reagents and solutions and all the assay conditions required for their production. The material is presented to enable research and development managers to make choices as to which are the most suitable techniques for their requirements. The book is comprehensively referenced, and will be a necessary resource for all those who are involved with this technology.
Book Synopsis Monoclonal Antibodies by : Johann H. Peters
Download or read book Monoclonal Antibodies written by Johann H. Peters and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present new version of this popular laboratory manual is at the same time the first one of this text in the English language - and this makes me even a little proud, as it reminds me of probably the first collection of monoclonal recipes in English, written by myself, which circulated for a couple of years in many laboratories. In the meantime many researchers have put enormous effort into improving methods for monoclonal antibody production. The proce dures have become more and more standardized and by this have more and more lost the character of magic secrets. Hinrich Peters and Horst Baumgarten, who had followed this good tradition already in the previous edition, written in German, suc ceeded in making laboratory tricks teachable. They had contributed their own experiences in cell culture and immunology, and were able to engage a number of experienced authors to contribute to the work. They were all willing to follow the general concept of this book, which contains a brief theoretical background for the methods described and presents the procedures in a highly organized structure. So the book has retained its shape as a "cook-book", which I especially like.
Book Synopsis Monoclonal Antibodies by : Maher Albitar
Download or read book Monoclonal Antibodies written by Maher Albitar and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.
Book Synopsis Monoclonal Antibodies in Biotechnology by : Kenneth C. McCullough
Download or read book Monoclonal Antibodies in Biotechnology written by Kenneth C. McCullough and published by Cambridge University Press. This book was released on 1990-04-19 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry.
Book Synopsis Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas by : A.M. Campbell
Download or read book Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas written by A.M. Campbell and published by Elsevier. This book was released on 2000-04-01 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
Book Synopsis Exquisite Specificity by : Alberto Cambrosio
Download or read book Exquisite Specificity written by Alberto Cambrosio and published by New York : Oxford University Press. This book was released on 1995 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Based on an extensive reading of the literature in the field, interviews with leading participants and on-site observation, the book follows events, materials and actors through a twenty-year period during which a laboratory technique was transformed into one of the most successful tools of modern biotechnology. Beginning with an analysis of the ambiguities surrounding the initial status of monoclonal antibodies, the book goes on to explore the various practices and forms of knowledge involved in the reproduction of hybridoma technology. The analysis of the worldwide diffusion of monoclonal antibodies and their consequent transformation by users is complemented by a close-up account of their adoption within two laboratories.
Author :Committee on Methods of Producing Monoclonal Antibodies Publisher :National Academies Press ISBN 13 :0309519047 Total Pages :74 pages Book Rating :4.3/5 (95 download)
Book Synopsis Monoclonal Antibody Production by : Committee on Methods of Producing Monoclonal Antibodies
Download or read book Monoclonal Antibody Production written by Committee on Methods of Producing Monoclonal Antibodies and published by National Academies Press. This book was released on 1999-05-20 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) in early 1997 to prohibit the use of an animal in the production of mAb (monoclonal antibodies). NIH responded late in 1997, asserting that continued use of the mouse method for producing mAb was scientifically required. In a second petition, in early 1998, AAVS did not accept the NIH response. NIH asked the National Research Council to form a committee to study this issue. The Committee on Methods of Producing Monoclonal Antibodies was composed of 11 experts with extensive experience in biomedical research, laboratory animal medicine, pain research, animal welfare, and patient advocacy. The committee was asked to determine whether there is a scientific necessity for producing mAb by the mouse method and, if so, to recommend ways to minimize any pain or distress that might be associated with the method. The committee was also to determine whether there are regulatory requirements for the mouse method and to summarize the current stage of development of tissue-culture methods.
Book Synopsis Methods of Hybridoma Formation by : Arie H. Bartal
Download or read book Methods of Hybridoma Formation written by Arie H. Bartal and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laymen often consider modern laboratory research to be based on an endless array of sophisticated technologies whose complex capabilities are as important to the outcome of any project as the inventiveness and creativity of the scientists who employ them. Scientists at times may share this point of view until they are con fronted by unexpected findings that demand new approaches, and they discover that yesterday's "sophisticated tools" are today's "blunt instruments." This experience provides a more sobering view of the current state of our scientific methods. It also serves as an impetus for the further development of technology that prepares us for the next stage of advance. Immunologists were confronted by such a technological crises in the late 1970s when they finally were forced to admit that poly clonal antibodies, although quite sensitive reagents, were not spe cific enough to answer many of the questions then confronting virologists and tumor biologists. The answer to the need for specific ity came with the development of monoclonal antibody technology. In the last ten years there have been considerable advances in monoclonal antibody techniques. Today these reagents are much more versatile than they were initially and can be applied to a broad range of problems. Still, most workers who are using these anti bodies are convinced that their potential is far from exhausted, and that at least in some fields we are currently in the early stages of learning how to use them properly.
