Molecular Diagnostic Methods For Bacteria And Fungi Detection

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Molecular diagnostic methods for bacteria and fungi detection

Author : Laura Cerqueira
Publisher : Frontiers Media SA
ISBN 13 : 2832550266
Total Pages : 100 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Molecular diagnostic methods for bacteria and fungi detection by : Laura Cerqueira

Download or read book Molecular diagnostic methods for bacteria and fungi detection written by Laura Cerqueira and published by Frontiers Media SA. This book was released on 2024-06-12 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microorganisms, like bacteria and fungi, are ubiquitous worldwide and can have different roles in human’s lives. Some will bring beneficial effects which are exploited and used in industrial and agricultural sectors. Contrariwise, some are responsible for several life-threatening diseases. Microbial analysis, surveillance and research is therefore crucial. Until recently, the classical culturing methods were widely used to study bacteria and fungi. However these methods, although considered the gold standard, are becoming now obsolete since they tend to be time-consuming, have low sensitivity and are unable to detect some cellular morphological states, as the viable but non-culturable (VBNC) state, leading to false negative results. Moving away from the classical methods, microbial detection is now evolving to new effective and rapid diagnostics.

Molecular Methods for Microbial Identification and Typing

Author : K.J. Towner
Publisher : Springer Science & Business Media
ISBN 13 : 9401115060
Total Pages : 208 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Molecular Methods for Microbial Identification and Typing by : K.J. Towner

Download or read book Molecular Methods for Microbial Identification and Typing written by K.J. Towner and published by Springer Science & Business Media. This book was released on 2013-03-07 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: The accurate identification and typing of microbes is essential for workers active in all fields of microbiology. Many examples of modern molecular methods have been concealed in scientific and medical literature but this introductory text considers the possible applications of such methods and compares their advantages and disadvantages.

Diagnostic Bacteriology Protocols

Author : Louise O’Connor
Publisher : Springer Science & Business Media
ISBN 13 : 1597451436
Total Pages : 226 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Diagnostic Bacteriology Protocols by : Louise O’Connor

Download or read book Diagnostic Bacteriology Protocols written by Louise O’Connor and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.

Evolving Landscape of Molecular Diagnostics

Author : Mrutyunjay Suar
Publisher : Elsevier
ISBN 13 : 0323993176
Total Pages : 276 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Evolving Landscape of Molecular Diagnostics by : Mrutyunjay Suar

Download or read book Evolving Landscape of Molecular Diagnostics written by Mrutyunjay Suar and published by Elsevier. This book was released on 2024-06-27 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Evolving Landscape of Molecular Diagnostics: Applications and Techniques presents cutting-edge reviews on current and emerging technologies in the diagnosis of microbial infections. The book discusses the fundamentals of Molecular Diagnostics for bacteria, fungi, viruses and a variety of state-of-the-art diagnostics tools and techniques for obtaining qualitative and quantitative results. It includes topics such as Next generation sequencing and application of "OMICS" for early disease diagnosis. Sections cover the entire spectrum of emerging diagnostic tools and techniques, as well as the principles, pros and cons of each method and applications for diagnosis of infectious disease in plants, humans and veterinary. This is a resource for both researchers and students working in clinical microbiology, infectious biology, applied life sciences and scientists working in the clinical diagnostic industry. - Provides a wide range of diagnostics tools and technologies in the field of infectious biology - Presents discussions on emerging technologies for early disease diagnosis and novel diagnostics techniques for detection of COVID, Urea Breath Tests for Detection of Helicobacter pylori, Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis, Molecular imaging, Lab-on-a-chip Technologies, and several others - Analyzes literature reviews, underpinning methodologies, and opportunities and limitations - Includes case studies and examples that highlight applications using the underpinning techniques

Molecular Microbial Diagnostic Methods

Author : Nigel Cook
Publisher : Academic Press
ISBN 13 : 0124171702
Total Pages : 268 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Molecular Microbial Diagnostic Methods by : Nigel Cook

Download or read book Molecular Microbial Diagnostic Methods written by Nigel Cook and published by Academic Press. This book was released on 2015-10-06 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Microbial Diagnostic Methods: Pathways to Implementation for the Food and Water Industry was developed by recognized and experienced highlevel scientists. It's a comprehensive and detailed reference that uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. This book discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The content is also pertinent for anyone carrying out microbiological analysis at the research level, and for scientists developing methods, as it focuses on the requirements of end-users. - Includes information on how to introduce and implement molecular methods for routine monitoring in food and water laboratories - Discusses the importance of robust validation of molecular methods as alternatives to existing standard methods to help ensure the production of defendable results - Highlights potential issues with respect to successful implementation of these methods

Molecular Microbiology

Author : David H. Persing
Publisher : John Wiley & Sons
ISBN 13 : 1555819079
Total Pages : 835 pages
Book Rating : 4.5/5 (558 download)

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Book Synopsis Molecular Microbiology by : David H. Persing

Download or read book Molecular Microbiology written by David H. Persing and published by John Wiley & Sons. This book was released on 2020-07-24 with total page 835 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.

Molecular Diagnostics

Author : E. van Pelt-Verkuil
Publisher : Springer
ISBN 13 : 9811045119
Total Pages : 241 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Molecular Diagnostics by : E. van Pelt-Verkuil

Download or read book Molecular Diagnostics written by E. van Pelt-Verkuil and published by Springer. This book was released on 2017-07-19 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: This two volume book set provides a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are currently used in a wide variety of disciplines. The volumes cover: Clinical microbiology and virology Clinical chemistry Pathology Veterinary medicine Plant Pathology Food safety The two volumes are written by experts in their respective fields, who have, together with the editors, combined years of experience in the development, application and quality control of molecular diagnostic methods. The first book is devoted to the theory and backgrounds of molecular techniques, amplification technology, next generation sequencing and bioinformatics for molecular laboratory diagnostics. As a fundament of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is extensively discussed. The second book highlights the applications of these methods in the various diagnostic laboratories. These two full-colour well-illustrated volumes are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic tests and for those who wish to expand their knowledge on the current molecular biological revolution. The extensive information in both books highlights the current trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.

Molecular Methods in Plant Disease Diagnostics

Author : Neil Boonham
Publisher : CABI
ISBN 13 : 1780641478
Total Pages : 214 pages
Book Rating : 4.7/5 (86 download)

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Book Synopsis Molecular Methods in Plant Disease Diagnostics by : Neil Boonham

Download or read book Molecular Methods in Plant Disease Diagnostics written by Neil Boonham and published by CABI. This book was released on 2016-04-19 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.

Oxford Textbook of Medical Mycology

Author : Christopher C. Kibbler
Publisher : Oxford University Press
ISBN 13 : 0198755384
Total Pages : 401 pages
Book Rating : 4.1/5 (987 download)

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Book Synopsis Oxford Textbook of Medical Mycology by : Christopher C. Kibbler

Download or read book Oxford Textbook of Medical Mycology written by Christopher C. Kibbler and published by Oxford University Press. This book was released on 2017-12-14 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Oxford Textbook of Medical Mycology is a comprehensive reference text which brings together the science and medicine of human fungal disease. Written by a leading group of international authors to bring a global expertise, it is divided into sections that deal with the principles of mycology, the organisms, a systems based approach to management, fungal disease in specific patient groups, diagnosis, and treatment. The detailed clinical chapters take account of recent international guidelines on the management of fungal disease. With chapters covering recent developments in taxonomy, fungal genetics and other 'omics', epidemiology, pathogenesis, and immunology, this textbook is well suited to aid both scientists and clinicians. The extensive illustrations, tables, and in-depth coverage of topics, including discussion of the non-infective aspects of allergic and toxin mediated fungal disease, are designed to aid the understanding of mechanisms and pathology, and extend the usual approach to fungal disease. This textbook is essential reading for microbiologists, research scientists, infectious diseases clinicians, respiratory physicians, and those managing immunocompromised patients. Part of the Oxford Textbook in Infectious Disease and Microbiology series, it is also a useful companion text for students and trainees looking to supplement mycology courses and microbiology training.

Fungal Diagnostics

Author : Louise O'Connor
Publisher : Humana Press
ISBN 13 : 9781493959808
Total Pages : 228 pages
Book Rating : 4.9/5 (598 download)

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Book Synopsis Fungal Diagnostics by : Louise O'Connor

Download or read book Fungal Diagnostics written by Louise O'Connor and published by Humana Press. This book was released on 2016-05-01 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology book focuses specifically on fungal detection in clinical settings, including fungal diagnostics, environmental testing, agriculture and food production and veterinary diagnostics. Offers materials lists, tips and protocols."

Global Infectious Disease Surveillance and Detection

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309111145
Total Pages : 284 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Global Infectious Disease Surveillance and Detection by : Institute of Medicine

Download or read book Global Infectious Disease Surveillance and Detection written by Institute of Medicine and published by National Academies Press. This book was released on 2007-11-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.

Developing Norms for the Provision of Biological Laboratories in Low-Resource Contexts

Author : National Academies of Sciences, Engineering, and Medicine
Publisher : National Academies Press
ISBN 13 : 0309486564
Total Pages : 121 pages
Book Rating : 4.3/5 (94 download)

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Book Synopsis Developing Norms for the Provision of Biological Laboratories in Low-Resource Contexts by : National Academies of Sciences, Engineering, and Medicine

Download or read book Developing Norms for the Provision of Biological Laboratories in Low-Resource Contexts written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2019-05-01 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: On June 27-28, 2018, the U.S. National Academies of Sciences, Engineering, and Medicine (the National Academies) convened an international workshop in Amsterdam, the Netherlands, on developing norms for the provision of laboratories in low-resource contexts. The U.S. Department of State's Biosecurity Engagement Program requested that the National Academies organize this workshop to engage an international group of organizations that provide funding for construction, upgrades, and maintenance of biological laboratories in countries without the means to build such labs themselves. Twenty-one people from 19 organizations participated. The intent was to advance the conversation about the identification and application of guiding principles and common norms for use by these organizations in their grants, partnerships, and aid. This publication summarizes the presentations and discussions from the workshop.

Advanced Techniques in Diagnostic Microbiology

Author : Yi-Wei Tang
Publisher : Springer Science & Business Media
ISBN 13 : 1461439698
Total Pages : 943 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang

Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer Science & Business Media. This book was released on 2012-09-13 with total page 943 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the United States, hospitals annually report over 5 million cases of infectious-disease-related illnesses: clinical microbiology laboratories in these hospitals are engaged in detecting and identifying the pathogenic microorganisms in clinical specimens collected from these patients with suspected infections. Clearly, the timely and accurate detection/identification of these microbial pathogens is critical for patient treatment decisions and outcomes for millions of patients each year. Despite an appreciation that the outcome of an infectious-disease-related illness is directly related to the time required to detect and identify a microbial pathogen, clinical microbiology laboratories in the United States as well as worldwide have long been hampered by traditional culture-based assays, which may require prolonged incubation time for slowly growing microorganisms such as Mycobacterium tuberculosis. Moreover, traditional culture-based assays often require multiple steps with additional time needed for discernment of species and/or detection of antimicrobial resistance. Finally, these traditional, slow multistep culture-based assays are labor-intensive and required skilled clinical microbiologists at the bench. Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition again provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a “techniques” section describing the latest molecular techniques and an “applications” section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.

Diagnostic Bacteriology Protocols

Author : Louise O’Connor
Publisher : Humana Press
ISBN 13 : 9781588295941
Total Pages : 226 pages
Book Rating : 4.2/5 (959 download)

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Book Synopsis Diagnostic Bacteriology Protocols by : Louise O’Connor

Download or read book Diagnostic Bacteriology Protocols written by Louise O’Connor and published by Humana Press. This book was released on 2006-04-21 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.

Molecular Identification of Fungi

Author : Youssuf Gherbawy
Publisher : Springer Science & Business Media
ISBN 13 : 3642050425
Total Pages : 511 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis Molecular Identification of Fungi by : Youssuf Gherbawy

Download or read book Molecular Identification of Fungi written by Youssuf Gherbawy and published by Springer Science & Business Media. This book was released on 2010-03-03 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi enjoy great popularity in pharmaceutical, agricultural, and biotechnological applications. Recent advances in the decipherment of whole fungal genomes promise an acceleration of these trends. This timely book links scientists from different parts of the world who are interested in the molecular identification of fungi combined with the exploration of the fungal biodiversity in different ecosystems. It provides a compendium for scientists who rely on a rapid and reliable detection of fungal specimens in environmental as well as clinical resources in order to ensure the benefit of industrial and clinical applications. Chapters focus on the opportunities and limits of the molecular marker-mediated identification of fungi. Various methods, procedures and strategies are outlined. Furthermore, the book offers an update of the current progress in the development of fungal molecular techniques, and draws attention to potential and associated problems, as well as integrating theory and practice.

Identification of Pathogenic Fungi

Author : Colin K. Campbell
Publisher : John Wiley & Sons
ISBN 13 : 1118520076
Total Pages : 351 pages
Book Rating : 4.1/5 (185 download)

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Book Synopsis Identification of Pathogenic Fungi by : Colin K. Campbell

Download or read book Identification of Pathogenic Fungi written by Colin K. Campbell and published by John Wiley & Sons. This book was released on 2013-01-25 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of Identification of Pathogenic Fungi, there has been incredible progress in the diagnosis, treatment and prevention of fungal diseases: new methods of diagnosis have been introduced, and new antifungal agents have been licensed for use. However, these developments have been offset by the emergence of resistance to several classes of drugs, and an increase in infections caused by fungi with innate resistance to one or more classes. Identification of Pathogenic Fungi, Second Edition, assists in the identification of over 100 of the most significant organisms of medical importance. Each chapter is arranged so that the descriptions for similar organisms may be found on adjacent pages. Differential diagnosis details are given for each organism on the basis of both colonial appearance and microscopic characteristics for the organisms described. In this fully updated second edition, a new chapter on the identification of fungi in histopathological sections and smears has been added, while colour illustrations of cultures and microscopic structures have been included, and high quality, four colour digital images are incorporated throughout.

Molecular Detection of Human Bacterial Pathogens

Author : Dongyou Liu
Publisher : CRC Press
ISBN 13 : 143981239X
Total Pages : 1258 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Molecular Detection of Human Bacterial Pathogens by : Dongyou Liu

Download or read book Molecular Detection of Human Bacterial Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2011-04-18 with total page 1258 pages. Available in PDF, EPUB and Kindle. Book excerpt: As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses th