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Molecular Cloning And Functional Characterization Of Goldfish Alpha 2 Adrenergic Receptors
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Book Synopsis Molecular Cloning and Functional Characterization of Goldfish Alpha-2 Adrenergic Receptors by : Hoi-yan Chan (M.Phil.)
Download or read book Molecular Cloning and Functional Characterization of Goldfish Alpha-2 Adrenergic Receptors written by Hoi-yan Chan (M.Phil.) and published by . This book was released on 2004 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Cloning and Functional Characterization of Goldfish Alpha-2 Adrenergic Receptors by : Hoi-Yan Chan
Download or read book Molecular Cloning and Functional Characterization of Goldfish Alpha-2 Adrenergic Receptors written by Hoi-Yan Chan and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Molecular Cloning and Functional Characterization of Goldfish Alpha-2 Adrenergic Receptors" by Hoi-yan, Chan, 陳凱恩, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled MOLECULAR CLONING AND FUNCTIONAL CHARACTERIZATION OF GOLDFISH ALPHA-2 ADRENERGIC RECEPTORS Submitted by Chan Hoi Yan for the degree of Master of Philosophy at The University of Hong Kong in September 2004 In mammals, adrenergic stimulation at the hypothalamic level through alpha-2 adrenergic receptors can induce growth hormone secretion from the pituitary. However, the alpha-2 receptor subtype(s) responsible for this stimulatory action has not been elucidated. In the goldfish, we have previously shown that alpha-2 activation at the pituitary level can suppress growth hormone release and that removal of alpha-2 inhibition can trigger a rebound of growth hormone secretion. These findings have prompted us to speculate that alpha-2 adrenergic receptors should be expressed in goldfish pituitary cells and be responsible for the growth hormone regulation by adrenergic input at the pituitary level. As a first step to study the alpha-2 receptor subtype(s) expressed in the goldfish pituitary, molecular cloning was performed to establish the structural identity of the goldfish alpha-2A, alpha-2B, and alpha-2C adrenergic receptors. By library screening and 3'/5' RACE, the complementary DNAs (cDNAs) for alpha-2A, alpha-2B, and alpha-2C adrenergic receptors with the sizes of 2.72 Kb, 2.86 Kb, and 1.72 Kb, respectively, have been isolated from the brain-pituitary axis of the goldfish. The open-reading frames of these cDNAs encode proteins of 383 to 505 amino acids in size with seven transmembrane domains typical of those of the G protein-coupled receptors. Sequence analysis also reveals that these goldfish alpha-2A, alpha-2B, and alpha-2C adrenergic receptors are structurally homologous to the corresponding receptor subtypes in mammals. By reverse transcription polymerase chain reaction (RT-PCR), the transcripts of alpha-2A and alpha-2B adrenergic receptors were identified in many tissues in the goldfish, including the heart, liver, gill, intestine, kidney, muscle, spleen, pituitary, and various brain segments. A similar expression pattern was also noted for alpha-2C adrenergic receptor, except that its mRNA was not detected in the intestine and its expression in the brain was restricted to the brain stem and spinal cord. To characterize the pharmacological and biochemical properties of these newly cloned receptors, functional expression of goldfish alpha-2A, alpha-2B, and alpha-2C adrenergic receptors was conducted in Chinese hamster ovary (CHO) cells. In this case, the receptor binding properties of these goldfish adrenergic receptors were tested using a radioreceptor binding approach. Although the order of affinity of the respective alpha-2A, alpha-2B, and alpha-2C adrenergic receptors is different from that reported in mammals, these receptors consistently exhibited a high affinity for ligands selective for alpha-2 but not for alpha-1 and beta adrenergic receptors. Furthermore, activation of these receptors by norepinephrine or the alpha-2 agonist + + clonidine could trigger rapid activation of the Na /H exchanger, an inhibition of 2+ cyclic AMP production and a decrease in intracellular Ca levels. These results, taken together, have confirmed that these newly cloned receptors are functional alpha-2 adrenergic receptors in the goldfish. The cDNAs for the goldfish alpha-2A, alpha-2B, and alpha-2C
Book Synopsis Molecular Cloning and Functional Characterization of a Goldfish Glucagon-Like Receptor by : 莫佩兒
Download or read book Molecular Cloning and Functional Characterization of a Goldfish Glucagon-Like Receptor written by 莫佩兒 and published by . This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Cloning and Functional Characterization of a Goldfish Glucagon-like Receptor by : Pui-yee Mok
Download or read book Molecular Cloning and Functional Characterization of a Goldfish Glucagon-like Receptor written by Pui-yee Mok and published by . This book was released on 1997 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Cloning and Functional Characterization of a Goldfish Growth Hormone-Releasing Hormone Receptor by : 陳冠榮
Download or read book Molecular Cloning and Functional Characterization of a Goldfish Growth Hormone-Releasing Hormone Receptor written by 陳冠榮 and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Molecular Cloning and Functional Characterization of a Goldfish Growth Hormone-releasing Hormone Receptor" by 陳冠榮, Koon-wing, Chan, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3121392 Subjects: Molecular cloning Growth hormone releasing factor Hormone receptors Goldfish - Physiology
Book Synopsis Molecular Cloning and Functional Characterization of a Goldfish Growth Hormone-releasing Hormone Receptor by : Koon-wing Chan
Download or read book Molecular Cloning and Functional Characterization of a Goldfish Growth Hormone-releasing Hormone Receptor written by Koon-wing Chan and published by . This book was released on 1996 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Cloning and Functional Characterization of a Goldfish Pituitary Adenylate Cyclase Activating Polypeptide Receptor by : Ling-cheung Shea (William)
Download or read book Molecular Cloning and Functional Characterization of a Goldfish Pituitary Adenylate Cyclase Activating Polypeptide Receptor written by Ling-cheung Shea (William) and published by . This book was released on 1998 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis MOLECULAR CLONING & CHARACTERI by : 許建熙
Download or read book MOLECULAR CLONING & CHARACTERI written by 許建熙 and published by Open Dissertation Press. This book was released on 2017-01-27 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Molecular Cloning and Characterization of Goldfish (Carassius Auratus) Mu-opioid Receptor" by 許建熙, Kin-hi, Raymond, Hui, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3122445 Subjects: Opioids - Receptors Goldfish - Genetics Molecular biology
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1828 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification and Functional Characterization of Two Novel [alpha]1-adrenergic Receptor-interacting Proteins by : Peter A. Clapp
Download or read book Identification and Functional Characterization of Two Novel [alpha]1-adrenergic Receptor-interacting Proteins written by Peter A. Clapp and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Ongoing Research in Jawed Fish Immunity: Structural and Functional Studies at the Protein and Cellular Levels by : Monica Imarai
Download or read book Ongoing Research in Jawed Fish Immunity: Structural and Functional Studies at the Protein and Cellular Levels written by Monica Imarai and published by Frontiers Media SA. This book was released on 2020-06-04 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of jawed fish immune systems obtained in the last 15 years has been mostly obtained through the sequencing of genomes of different fish species and from the use of high-throughput techniques such as transcriptomic analysis and RNA sequencing, which has allowed characterization of immune gene responses at the transcriptomic level. Although these have been important tools for exploring the complexities of the immune responses of fish species, the next generation of knowledge requires the use of new tools and methods capable of unveiling the diversity of immune cells and molecules in jawed fish, the network of interactions and responses, and the mechanisms leading to immune protection against pathogens. For more than 10 years, many laboratories have been engaged in developing antibodies against key molecules of jawed fish immune systems in several different species because the lack of sequence conservation of most immune genes makes it impossible to use the large panel of antibodies against mammalian molecules for recognition of fish molecules. The numerous genes encoding cytokines and receptors have now been used to make recombinant proteins available, allowing studies of the complex network of cytokine and receptor functions central to fish immune responses. From many laboratories, new and interesting knowledge about immune cells, their functions and interactions can be studied with the availability of new tools and methods have begun to provide a clear understanding fish immunity at the protein and cellular levels. This Research Topic gives a comprehensive overview of the current knowledge of jawed fish immune responses with a particular emphasis on structural and functional studies at the protein and cellular levels.
Book Synopsis Knobil and Neill's Physiology of Reproduction by : Ernst Knobil
Download or read book Knobil and Neill's Physiology of Reproduction written by Ernst Knobil and published by Gulf Professional Publishing. This book was released on 2006 with total page 1780 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 3rd edition, the first new one in ten years, includes coverage of molecular levels of detail arising from the last decade's explosion of information at this level of organismic organization. There are 5 new Associate Editors and about 2/3 of the chapters have new authors. Chapters prepared by return authors are extensively revised. Several new chapters have been added on the topic of pregnancy, reflecting the vigorous investigation of this topic during the last decade.The information covered includes both human and experimental animals; basic principels are sought, and information at the organismic and molecular levels are presented. *The leading comprehensive work on the physiology of reproduction*Edited and authored by the world's leading scientists in the field*Is a synthesis of the molecular, cellular, and organismic levels of organization*Bibliogrpahics of chapters are extensive and cover all the relevant literature
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2160 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Book Synopsis Hormones and Reproduction of Vertebrates by : David O. Norris
Download or read book Hormones and Reproduction of Vertebrates written by David O. Norris and published by Academic Press. This book was released on 2010-11-25 with total page 1681 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series of volumes represents a comprehensive and integrated treatment of reproduction in vertebrates from fishes of all sorts through mammals. It is designed to provide a readable, coordinated description of reproductive basics in each group of vertebrates as well as an introduction to the latest trends in reproductive research and our understanding of reproductive events. Whereas each chapter and each volume is intended to stand alone as a review of that topic or vertebrate group, respectively, the volumes are prepared so as to provide a thorough topical treatment across the vertebrates. Terminology has been standardized across the volumes to reduce confusion where multiple names exist in the literature, and a comprehensive glossary of these terms and their alternative names is provided. A complete, essential and up to date reference for research scientists working on vertebrate hormones and reproduction - and on animlals as models in human reproductive research Covers the endocrinology, neuroendocrinology, physiology, behaviour and anatomy of vertebrate reproduction Structured coverage of the major themes for all five vertebrate groups allows a consistent treatment for all Special chapters elaborate on features specific to individual vertebrate groups and to comparative aspects, similarities and differences between them
Book Synopsis Hormones and Reproduction of Vertebrates, Volume 1 by : David Norris
Download or read book Hormones and Reproduction of Vertebrates, Volume 1 written by David Norris and published by Academic Press. This book was released on 2011-05-04 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series of volumes represents a comprehensive and integrated treatment of reproduction in vertebrates from fishes of all sorts through mammals. It is designed to provide a readable, coordinated description of reproductive basics in each group of vertebrates as well as an introduction to the latest trends in reproductive research and our understanding of reproductive events. Whereas each chapter and each volume is intended to stand alone as a review of that topic or vertebrate group, respectively, the volumes are prepared so as to provide a thorough topical treatment across the vertebrates. Terminology has been standardized across the volumes to reduce confusion where multiple names exist in the literature, and a comprehensive glossary of these terms and their alternative names is provided. A complete, essential and up to date reference for research scientists working on vertebrate hormones and reproduction - and on animlals as models in human reproductive research Covers the endocrinology, neuroendocrinology, physiology, behaviour and anatomy of vertebrate reproduction Structured coverage of the major themes for all five vertebrate groups allows a consistent treatment for all Special chapters elaborate on features specific to individual vertebrate groups and to comparative aspects, similarities and differences between them
Book Synopsis Genetic Dissection of Important Traits in Aquaculture: Genome-scale Tools Development, Trait Localization and Regulatory Mechanism Exploration by : Peng Xu
Download or read book Genetic Dissection of Important Traits in Aquaculture: Genome-scale Tools Development, Trait Localization and Regulatory Mechanism Exploration written by Peng Xu and published by Frontiers Media SA. This book was released on 2020-07-31 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plasticity in the Vertebrate Pituitary, Including Regulatory Mechanisms by : Finn-Arne Weltzien
Download or read book Plasticity in the Vertebrate Pituitary, Including Regulatory Mechanisms written by Finn-Arne Weltzien and published by Frontiers Media SA. This book was released on 2021-07-14 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
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