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Molecular Biological Enhancement Of Coal Biodesulfurization Quarterly Technical Report September 1 1993 November 30 1993
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Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Quarterly Technical Report, September 1, 1993--November 30, 1993 by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Quarterly Technical Report, September 1, 1993--November 30, 1993 written by and published by . This book was released on 1993 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: IGT has developed a microbial culture of Rhodococcus rhodochrous, designated as IGTS8, that is capable of specifically cleaving carbon-sulfur bonds in a range of organosulfur model compounds and is capable of removing organic sulfur from coal and petroleum without significantly sacrificing the calorific value of the fuel. Although IGTS8 possesses the ability to specifically remove organic sulfur from coal, a major research need is to develop improved strains of microorganisms that possess higher levels of desulfurization activity and therefore will permit more favorable biodesulfurization process conditions: faster rates, more complete removal, and smaller reactor size. strain improvement is the single most important aspect to the development of a practical coal biodesulfurization process and accordingly is the focus of research in this project. During this quarter the promoter probe vectors that were constructed last quarter were found to be unstable in E. coli. Fragments of R. rhodochrous IGTS8 chromosomal DNA were cloned into pRCAT3 and pRCM1 (previously described in final ICCI report 1993). Many derivatives of pRCM1 and pRCAT3 receiving inserts that regulated the expression of chloramphenicol resistance in Rhodococcus rhodochrous IGTS8 proved to be unstable in E. coli frequently yielding plasmids containing deletions. Stable inserts have been observed ranging from 100 bp to 2.0 kb that regulated expression in Rhodococcus rhodochrous IGTS8. Subtractive hybridization studies continue, several candidates have been isolated and are being confirmed for inducible promoters. Primer extension analysis of the Rhodococcus rhodochrous IGTS8 16S RNA promoter region was initiated this quarter.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Final Technical Report, September 1, 1993--August 31, 1994 by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Final Technical Report, September 1, 1993--August 31, 1994 written by and published by . This book was released on 1994 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Energy Research Abstracts written by and published by . This book was released on 1995 with total page 782 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. [Quarterly] Technical Report, December 1, 1993--February 28, 1994 by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. [Quarterly] Technical Report, December 1, 1993--February 28, 1994 written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: IGT has developed a microbial culture of Rhodococcus rhodochrous, IGTS8, that is capable of specifically cleaving carbon-sulfur bonds in a range of organosulfur model compounds and is capable of removing organic sulfur from coal and petroleum. Although IGTS8 possesses the ability to specifically remove organic sulfur from coal, a major research need is to develop improved strain's of microorganisms that possess higher levels of desulfurization activity and therefore wall permit more favorable biodesulfurization process conditions: faster rates, mare complete removal, and smaller reactor size. Strain improvement is the single most important aspect to the development of a practical coal biodesulfurization process and accordingly is the focus of research in this project. Several possible strong promoters have been isolated and are in the process of being analyzed. When these promoters have been characterized for inducibility, strength, transcriptional start sites and other physical properties, they will be placed in front of the desulfurization genes and expression will be monitored. Improved promoter probe vectors have been constructed, allowing a conclusive screen of all putative Rhodococcus promoters. With the improved methodologies in the handling of Rhodococcus RNA, we have begun to gauge promoter expression using Northern blots. During this quarter we have constructed and successfully used a promoter probe vector using the [beta]-galactosidane gene from E. coli. A chromosomal promoter library was constructed upstream from the [beta]-galactosidase gene. Over 200 colonies were isolated that yielded [beta]-galactosidase activity.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Tenth Quarterly Technical Progress Report, [September--December 1991]. by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Tenth Quarterly Technical Progress Report, [September--December 1991]. written by and published by . This book was released on 1991 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The specific technical objectives of the project are to: clone and characterize the genes encoding the enzymes of the ''4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fast-growing chemolithotrophic bacterium; and conduct a batch-mode optimization/analysis of scale-up variables.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Eleventh Quarterly Technical Progress Report by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Eleventh Quarterly Technical Progress Report written by and published by . This book was released on 1992 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: (1) clone and characterize the genes encoding the enzymes of the ''4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; (2) return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; (3) transfer this pathway into a fast-growing chemolithotrophic bacterium; (4) conduct a batch-mode optimization/analysis of scale-up variables. By letter of September 3, 1991, from the Project Manager at Department of Energy, Pittsburgh Energy Technology Center, these objectives of this project were redirected toward finding and developing suitable vectors for Thiobacillus strains. All work on bacterial strains from Lehigh University was terminated since they did not contain desulfurization traits represented by the ''4S'' pathway.
Book Synopsis Government Reports Annual Index by :
Download or read book Government Reports Annual Index written by and published by . This book was released on 1995 with total page 1686 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sections 1-2. Keyword Index.--Section 3. Personal author index.--Section 4. Corporate author index.-- Section 5. Contract/grant number index, NTIS order/report number index 1-E.--Section 6. NTIS order/report number index F-Z.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Technical Report, March 1, 1994--May 31, 1994 by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Technical Report, March 1, 1994--May 31, 1994 written by and published by . This book was released on 1994 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: IGT has developed a microbial culture of Rhodococcus rhodochrous, designated as IGTS8, that is capable of specifically cleaving carbon-sulfur bonds in a range of organosulfur model compounds and is capable of removing organic sulfur from coal and petroleum without significantly sacrificing the calorific value of the fuel. Although IGTS8 possesses the ability to specifically remove organic sulfur from coal, a major research need is to develop improved strains of microorganisms that possess higher levels of desulfurization activity and therefore will permit more favorable biodesulfurization process conditions: faster rates, more complete removal, and smaller reactor size. Strain improvement is the single most important aspect to the development of a practical coal biodesulfurization process and accordingly is the focus of research in this project. During this quarter the DNA sequences and transcriptional initiation sites of several promoters derived from the chromosome of Rhodococcus rhodochrous IGTS8 were determined. Several strong promoters have been used to replace the promoter of the desulfurization genes with the intent of obtaining cultures with enhanced levels of desulfurization activity. The evaluation of the ability of improved cultures to desulfurize coal will be evaluated during the final quarter of this project.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Third Quarterly Technical Progress Report by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Third Quarterly Technical Progress Report written by and published by . This book was released on 1990 with total page 21 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: Clone and characterize the genes encoding the enzymes of the ''4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; Return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; Transfer this pathway into a fast-growing chemolithotrophic bacterium; Conduct a batch-mode optimization/analysis of scale-up variables.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. First Quarterly Technical Progress Report by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. First Quarterly Technical Progress Report written by and published by . This book was released on 1989 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: Clone and characterize the genes encoding the enzymes of the ''4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fast-growing chemolithotropic bacterium; conduct a batch-mode optimization/analysis of scale-up variables.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Fourth Quarterly Technical Progress Report by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Fourth Quarterly Technical Progress Report written by and published by . This book was released on 1990 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the ''4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fast-growing chemolithotropic bacterium; conduct a batch-mode optimization/analysis of scale-up variables.
Book Synopsis Molecular Biological Enhancement of Coal Desulfurization. Eleventh Quarterly Technical Progress Report by :
Download or read book Molecular Biological Enhancement of Coal Desulfurization. Eleventh Quarterly Technical Progress Report written by and published by . This book was released on 1991 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the 4̀̀S̀̀ pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of the organism; transfer this pathway into a fast-growing chemolithotrophic bacterium; conduct a batch-mode optimization/analysis of scale-up variables.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Ninth Quarterly Technical Progress Report by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Ninth Quarterly Technical Progress Report written by and published by . This book was released on 1991 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the ''4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fast-growing chemolithotropic bacterium; conduct a batch-mode optimization/analysis of scale-up variables.
Book Synopsis Reactivity of Target Compounds for Chemical Coal Desulfurization. Quarterly Technical Report, September 1, 1993--November 30, 1993 by :
Download or read book Reactivity of Target Compounds for Chemical Coal Desulfurization. Quarterly Technical Report, September 1, 1993--November 30, 1993 written by and published by . This book was released on 1993 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt: This project seeks to determine the identity of representative organosulfur compounds removed by coal desulfurization reactions. Demineralized coals from the Illinois Basin Coal Sample Program are solvent extracted and the extracts fractionated to separate and concentrate the organosulfur compounds for analysis by Gas Chromatography/Mass Spectroscopy. After the sulfur compounds are characterized, the parent extracts will be subjected to reactions previously shown to reduce the organic sulfur content of Illinois coals. The treated extracts will be fractionated and analyzed for organosulfur content by the same method to determine which compounds reacted during the chemical treatment. Finally, the original coal will be subjected to chemical desulfurization, extraction, fractionation and analysis in order to correlate changes in organic sulfur content of the coal with reactions of specific sulfur compounds. These compounds can thus be reliably considered as target molecules for the next generation of desulfurization processes. Work during this quarter included studies to increase the amount of soluble material extracted from coal by several solvents and development of standard chromatographic methods for fractionation and analysis of soluble coal fractions. Pyridine solubility of several coals is enhanced by removal of mineral matter prior to extraction. The effect is modest (12% relative increase) for Illinois No. 6 coal and greater for lower rank coal. Ultrasound and swelling agents cause a large increase in pyridine solubility. Although toluene extracts much less material from Illinois coals than pyridine, removal of mineral matter prior to extraction causes a relatively larger increase in extraction yield (60 - 80% relative increase). Expected aromatic sulfur compounds such as dibenzothiophene have been detected by GC/MS analysis of non-polar fractions of Illinois No. 6 extracts and several oxidized thiophenes were detected in extracts of weathered IBC-105 coal.
Book Synopsis Government Reports Announcements & Index by :
Download or read book Government Reports Announcements & Index written by and published by . This book was released on 1994 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Final Technical Report by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Final Technical Report written by and published by . This book was released on 1992 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress is reported in understanding Thiobacillus molecular biology, specifically in the area of vector development. At the initiation of this program, the basic elements needed for performing genetic engineering in T. ferrooxidans were either not yet developed. Improved techniques are described which will make it easier to construct and analyze the genetic structure and metabolism of recombinant T. ferrooxidans. The metabolism of the model organic sulfur compound dibenzothiophene (DBT) by certain heterotrophic bacteria was confirmed and characterized. Techniques were developed to analyze the metabolites of DBT, so that individual 4S pathway metabolites could be distinguished. These techniques are expected to be valuable when engineering organic sulfur metabolism in Thiobacillus. Strain isolation techniques were used to develop pure cultures of T. ferrooxidans seven of which were assessed as potential recombinant hosts. The mixotrophic strain T. coprinus was also characterized for potential use as an electroporation host. A family of related Thiobacillus plasmids was discovered in the seven strains of P. ferrooxidans mentioned above. One of these plasmids, pTFI91, was cloned into a pUC-based plasmid vector, allowing it to propagate in E. coli. A key portion of the cloned plasmid was sequenced. This segment, which is conserved in all of the related plasmids characterized, contains the vegetative origin of DNA replication, and fortuitously, a novel insertion sequence, designated IS3091. The sequence of the DNA origin revealed that these Thiobacillus plasmids represent a unique class of replicons not previously described. The potentially useful insertion sequence IS3091 was identified as a new member of a previously undefined family of insertion sequences which include the E. coli element IS30.
Book Synopsis Molecular Biological Enhancement of Coal Biodesulfurization. Seventh Quarter Report, May--July 1990 by :
Download or read book Molecular Biological Enhancement of Coal Biodesulfurization. Seventh Quarter Report, May--July 1990 written by and published by . This book was released on 1990 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall objective of this project is to sue molecular genetics to develop strains of bacteria with enhanced ability to remove sulfur from coal and to obtain data that will allow the performance and economics of a coal biodesulfurization process to be predicted. The work planned for the current quarter (May 1990 to July 1990) includes the following activities: (1) Construct a cloning vector that can be used in Rhodococcus rhodochrous IGTS8 from the small cryptic plasmid found in Rhodococcus rhodochrous ATCC 190607; (2) Develop techniques for the genetic analysis of IGTS8; (3) Continue biochemical experiments, particularly those that may allow the identification of desulfurization-related enzymes; (4) Continue experiments with coal to determine the kinetics of organic sulfur removal.
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