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Author : Patrick Echlin
Publisher :
ISBN 13 :
Total Pages : 400 pages
Book Rating : 4.2/5 (258 download)
Download or read book Microprobe Analysis in Biology and Medicine written by Patrick Echlin and published by . This book was released on 1978 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Karl Zierold
Publisher : Springer Science & Business Media
ISBN 13 : 364274477X
Total Pages : 331 pages
Book Rating : 4.6/5 (427 download)
Download or read book Electron Probe Microanalysis written by Karl Zierold and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Author : Peter Ingram
Publisher :
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.3/5 (91 download)
Download or read book Microprobe Analysis in Medicine written by Peter Ingram and published by . This book was released on 1989 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: A work reviewing the applications of new microprobe analysis techniques in medicine. Authoritative chapters illustrate a variety of techniques and demonstrate appropriate applications to specific diseases. Key coverage includes pulmonary pathology and asbestoses.
Author : Yvan Llabador
Publisher : World Scientific
ISBN 13 : 9789810223625
Total Pages : 292 pages
Book Rating : 4.2/5 (236 download)
Download or read book Applications of Nuclear Microprobe in the Life Sciences written by Yvan Llabador and published by World Scientific. This book was released on 1998 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: "... this book will be particularly useful to the many biologists encountering problems of elemental analysis & analytical imaging who wish to have a clear yet comprehensive introduction to the principles, the conditions of application & the potential of nuclear microprobes compared with those of alternative methods." Journal of Trace & Microprobe Techniques, 1999
Author : Peter Ingram
Publisher : Academic Press
ISBN 13 : 0080524567
Total Pages : 573 pages
Book Rating : 4.0/5 (85 download)
Download or read book Biomedical Applications of Microprobe Analysis written by Peter Ingram and published by Academic Press. This book was released on 1999-10-29 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter
Author : Battelle Conference Center
Publisher :
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.3/5 (91 download)
Download or read book Microprobe Analysis of Biological Systems written by Battelle Conference Center and published by . This book was released on 1981 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microprobe Analysis of Biological Systems ...
Author : Claude Lechene
Publisher : Elsevier
ISBN 13 : 0323143342
Total Pages : 695 pages
Book Rating : 4.3/5 (231 download)
Download or read book Microbeam Analysis in Biology written by Claude Lechene and published by Elsevier. This book was released on 2012-12-02 with total page 695 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.
Author : Armand Verbueken
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (91 download)
Download or read book Application of Laser Microprobe Mass Analysis (LAMMA) to Biology and Medicine written by Armand Verbueken and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Roger Theisen
Publisher : Springer
ISBN 13 :
Total Pages : 186 pages
Book Rating : 4.F/5 ( download)
Download or read book Quantitative Electron Microprobe Analysis written by Roger Theisen and published by Springer. This book was released on 1965 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Electron "licroprobe X-!{ay Analyscr conceivcd b ' R C.\S'L\I: \G and A. Cl'!: '\ lEI( in 1949 has been developcd as an extremelv po\\'crful tool in spcctrochcmical analysis for a wide range of applications, ranging from qualitative elcmcntary distribution studies, to highly localiscd quantitatin analysis on a one micron scale. \\'ith the increasing number oi' versatile instruments, commcrcially available, the domain of applications - in metallurgy, solid state physics, mineralogy and geology, biology and medicine, arts and archeology - is rapidly expanding, particularly because reliable quantitative analyses can be achieved. It is well established that in multicomponent specimens, the relative x-ray intensity generated by the electron bombardment - i.e. the intensity ratio of the characteristic x-ray radiation emitted under identical experimental conditions by the specimen and a calibration standard - is not directly correlated to the elementary mass concentration. The use of a wide scale of carefully prepared homogeneous calibration standards is generally very tedious and restricted to binar)' systems. For more complex specimens, the conversion of recorded x-ra)' intensity ratios to elementary mass concentration requires, besides carefule selection of experimental conditions, an adequate correction calculation to take account oi' the various physical phenomenas occurring in the tarp;et - electron retardation, electron backseattering, x-ray excitation efficieney, fluorescence enhaneement by eharaeteristic and continuous radiation and x-ray mass absorption.
Author : David A. Erasmus
Publisher :
ISBN 13 :
Total Pages : 288 pages
Book Rating : 4.:/5 (42 download)
Download or read book Electron Probe Microanalysis in Biology written by David A. Erasmus and published by . This book was released on 1978 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : M.L. Wolbarsht
Publisher : Springer Science & Business Media
ISBN 13 : 1461307457
Total Pages : 307 pages
Book Rating : 4.4/5 (613 download)
Download or read book Laser Applications in Medicine and Biology written by M.L. Wolbarsht and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diversity of the chapters presented in this volume illustrates not only the many applications of lasers, but also the fact that, in many cases, these are not new uses of lasers, but rather improvements of laser techniques already widely accepted in both research and clinical situations. Biological reactions to some special aspects of laser exposure continue to show new effects, which have implications for the ever-present topic of laser safety. Such biological reactions are included in fields of research which depend on properties of electromagnetic radiation exposure only possible with lasers, for example, the short pulses necessary for the temperature-jump experiments reviewed by Reiss: Speciality lasers, such as the transverse excitation atmospheric (TEA) or excimer lasers, add new wavelengths and pulse domains to those already available for biological application. A description of these new types of lasers by Osgood is included to indicate new possibilities for future use and to avoid limiting our coverage to well-developed present-day applications. Hillenkamp and Kaufmann describe a microprobe mass spectrograph for analysis of the minute amounts of material evaporated by a laser pulse. The analytical possibilities of this instrument are far-reaching, and some of the various results are described to illustrate the power of their method, as well as to show the types of problems that are suitable for it. The initial steps in photosynthesis have become the subject of intensive investigation.
Author : Lylia Khassanova
Publisher : John Libbey Eurotext
ISBN 13 : 9782742004294
Total Pages : 692 pages
Book Rating : 4.0/5 (42 download)
Download or read book Metal Ions in Biology and Medicine written by Lylia Khassanova and published by John Libbey Eurotext. This book was released on 1990-05 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the Seventh International Symposium on Metal Ions in Biology & Medicine held in Saint Petersburg State University, Saint Petersburg, Russia, on 5-9 May 2002.
Author : U. Lüttge
Publisher : Springer Science & Business Media
ISBN 13 : 3642874967
Total Pages : 245 pages
Book Rating : 4.6/5 (428 download)
Download or read book Microautoradiography and Electron Probe Analysis written by U. Lüttge and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the light beam I transmitted by the film is in itself a measure of grain density if the luminous flux 10 incident on the grains is kept constant. This approach has been used in a large number of measuring setups.
Author : David C. Sigee
Publisher : Cambridge University Press
ISBN 13 : 9780521415309
Total Pages : 356 pages
Book Rating : 4.4/5 (153 download)
Download or read book X-ray Microanalysis in Biology written by David C. Sigee and published by Cambridge University Press. This book was released on 1993-04-22 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes an integrated approach to the use of X-ray microanalysis in biology.
Author : Theodore Alvin Hall
Publisher :
ISBN 13 :
Total Pages : 464 pages
Book Rating : 4.3/5 (91 download)
Download or read book Microprobe Analysis as Applied to Cells and Tissues written by Theodore Alvin Hall and published by . This book was released on 1974 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Symposium on Recent Developments in Research Methods and Instrumentation, October 2-6, 1967, at the Clinical Center Auditorium, National Institutes of Health, Bethesda, Md., Held in Conjunction with the Seventeenth Annual Research Equipment Exhibit written by and published by . This book was released on 1967 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Abraham Boekestein
Publisher : Springer Science & Business Media
ISBN 13 : 3709166799
Total Pages : 271 pages
Book Rating : 4.7/5 (91 download)
Download or read book Electron Microbeam Analysis written by Abraham Boekestein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: This supplement of Mikrochimica Acta contains selected papers from the Second Workshop of the European Microbeam Analysis Society (EMAS) "Modern Developments and Applications in Microbeam Analysis", on which took place in May 1991 in Dubrovnik (Yugoslavia). EMAS was founded in 1987 by members from almost all European countries, in order to stimulate research, applications and development of all forms of microbeam methods. One of the most important activities EMAS is the organisation of biannual workshops for demonstrating the current status and developing trends of microbeam methods. For this meeting, EMAS chose to highlight the following topics: electron-beam microanalysis (EPMA) of thin films and quantitative analysis of ultra-light elements, Auger electron spectroscopy (AES), electron energy loss spec trometry (EELS), high-resolution transmission electron microscopy (HRTEM), quantitative analysis of biological samples and standard-less electron-beam microanalysis. Seven introductory lectures and almost seventy poster presentations were given by speakers from twelve European and two non-European (U.S.A. and Argentina) countries were made. One cannot assume that all fields of research in Europe were duly represented, but a definite trend is discernible. EPMA with wavelength-dispersive spectrometry (WDS) or energy-dispersive spectrometry (EDS) is the method with by far the widest range of applications, followed by TEM with EELS and then AES. There are also interesting suggestions for the further development of new appa ratus with new fields of application. Applications are heavily biased towards materials science (thin films in microelectronics and semicon ductors), ceramics and metallurgy, followed by analysis of biological and mineral samples.
