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Author : François A. Roch
Publisher :
ISBN 13 :
Total Pages : 412 pages
Book Rating : 4.E/5 ( download)
Download or read book Microbial Degradation of Trichloroethylene and Toluene written by François A. Roch and published by . This book was released on 1995 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mark Edmund Fuller
Publisher :
ISBN 13 :
Total Pages : 402 pages
Book Rating : 4.:/5 (55 download)
Download or read book The Effects of Trichloroethylene (TCE) and Toluene on Soil Microbial Community Structure and Function written by Mark Edmund Fuller and published by . This book was released on 1995 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Der-Yi Mu
Publisher :
ISBN 13 :
Total Pages : 184 pages
Book Rating : 4.:/5 (53 download)
Download or read book Effect of Acclimation on Biodegradation of Trichloroethylene and Toluene by Soil Microbial Populations written by Der-Yi Mu and published by . This book was released on 1993 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Elica Monique Moss
Publisher :
ISBN 13 :
Total Pages : 290 pages
Book Rating : 4.3/5 (129 download)
Download or read book Microbial Community Structure in a Trichloroethylene Contaminated Aquifer During Toluene Stimulated Bioremediation written by Elica Monique Moss and published by . This book was released on 2004 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Nagarjuna Reddy Bommareddy
Publisher :
ISBN 13 :
Total Pages : 258 pages
Book Rating : 4.:/5 (345 download)
Download or read book Biodegradation of Trichloroethylene with Toluene as Cometabolite by Indigenous Microbial Populations in Soil written by Nagarjuna Reddy Bommareddy and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Astrid E. Mars
Publisher :
ISBN 13 : 9789036709811
Total Pages : 111 pages
Book Rating : 4.7/5 (98 download)
Download or read book Bacterial Degradation of Toluene, Chlorobenzene, and Trichloroethylene written by Astrid E. Mars and published by . This book was released on 1998 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zsfassung in niederländ. Sprache.
Author : Richard A. Snyder
Publisher :
ISBN 13 :
Total Pages : 12 pages
Book Rating : 4.3/5 (91 download)
Download or read book In Situ Bioremediation of Trichloroethylene Using Burkholderia Cepacia G4 PR1 written by Richard A. Snyder and published by . This book was released on 1998 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Alternative Substrates for Estimating TCE-degrading Capabilities of Toluene-oxidizing Bacteria written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the primary impediments to the implementation of bioremediation is uncertainty about success in the field. Soils and microbial populations are heterogeneous and it is difficult to extrapolate biodegradation rates from small samples to field scale. While biodegradation rates can be estimated from microcosm studies, in situ methods offer a more meaningful gauge of resident microbial activity. One method used to estimate biodegradation rates in the field is the newly developed Push-Pull technique. While this technique can be conducted on site, it is normally not possible to use target pollutants as the reactive substrates. Consequently, alternative, benign reactive tracers must be used. Ideally, these alternative, reactive tracers interrogate the same enzyme systems that are responsible for the biodegradation of the target pollutant. The objective of this study was to develop a reactive tracer system that could be used to assess toluene-dependent trichloroethylene (TCE) degradation. Our approach has been to determine whether a series of pure strains of toluene-oxidizing bacteria (Burkholderia cepacia G4, Pseudomonas putida F1, Pseudomonas putida mt2, Pseudomonas mendocina KR1), each with different toluene-oxidizing enzymes systems, are capable of cometabolically oxidizing a series of eleven potential alternative substrates. These substrates include simple alkenes, alkanes, and cyclic alkanes. The kinetics (Ks and Vmax) of the biotransformation of these compounds have been determined. While oxidation products were observed for a number of these substrates in connection with one or more of the test organisms, isobutylene was co-oxidized by all test organisms. Oxidation of isobutylene by each organism yielded kinetics constants comparable to the corresponding kinetics of TCE degradation. The enzyme system expressed by Burkholderia cepacia G4 catalyzed the epoxidation of isobutylene while the remaining enzyme systems catalyzed allylic alcohol formation. Isobutylene.
Author :
Publisher :
ISBN 13 :
Total Pages : 11 pages
Book Rating : 4.:/5 (227 download)
Download or read book The Mechanism of Trichloroethylene Oxidation by Toluene Dioxygenase: Implications for Bioremediation written by and published by . This book was released on 1991 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: The oxidation of trichloroethylene (TCE) by toluene dioxygenase in vivo and in vitro has been investigated. In a previous study, the initial rate of TCE oxidation by Pseudomonas putida Fl declined rapidly. This was shown in vivo, in the present study, to be due to toluene dioxygenase-dependent activation of TCE to produce reactive intermediates. Carbon-14 label from metabolism of radioactive TCE was incorporated into proteins, small molecules, DNA, RNA, and lipid. Alkylation of proteins, including toluene dioxygenase, caused metabolic poisoning and the loss of TCE-degrading ability. P'. putida was able to recover from TCE-mediated cytotoxicity. Toluene dioxygenase enzyme components were purified from recombinant E. coli strains by the use of conventional chromatographic methods. The major products of TCE oxidation by purified toluene dioxygenase have been identified as formic acid and glyoxylic acid. An additional minor product was shown to result from the alkylation of reduced pyridine dinucleotide (NADPH) by a reactive TCE intermediate generated by the toluene dioxygenase catalyzed reaction. Experiments with 14C-TCE showed that enzyme inactivation was due to non-specific alkylation of the proteins by diffusible reactive intermediates. A recombinant E. coli expressing cloned toluene dioxygenase genes oxidized TCE. The rate of TCE oxidation by the E. coli strain was slower than that of P. putida Fl but the rates were sustained for a longer time. The use of recombinant strains or gratuitously induced P. putida Fl could have important implications for bioremediation. Furthermore, a greater understanding of the mechanism of toluene dioxygenase inactivation will be crucial for developing optimum systems for TCE-biotreatment.
Author : Michael T. Begonia
Publisher :
ISBN 13 :
Total Pages : 106 pages
Book Rating : 4.:/5 (548 download)
Download or read book Degradation of Trichloroethylene by Pseudomonas Putida F1 Grown on Toluene and Its Degradation Product Benzyl Alcohol written by Michael T. Begonia and published by . This book was released on 2003 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sachiyo Iwashita
Publisher :
ISBN 13 :
Total Pages : 302 pages
Book Rating : 4.:/5 (574 download)
Download or read book Enhancing Aerobic Biological Degradation of Trichloroethylene and Metal Lubricants written by Sachiyo Iwashita and published by . This book was released on 2004 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jessica R. Hanson
Publisher :
ISBN 13 :
Total Pages : 176 pages
Book Rating : 4.:/5 (62 download)
Download or read book Biodegradation of Toluene and Methyl Tert-butyl Ether (MTBE) by Pure Bacterial Cultures written by Jessica R. Hanson and published by . This book was released on 2001 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Justin Thomas Shingleton
Publisher :
ISBN 13 :
Total Pages : 330 pages
Book Rating : 4.:/5 (43 download)
Download or read book Degradation of Toluene and Trichloroethylene in a Radial Flow Reactor written by Justin Thomas Shingleton and published by . This book was released on 1998 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 204 pages
Book Rating : 4.:/5 (727 download)
Download or read book Comparative Plant Uptake and Microbial Degradation of Trichloroethylene in the Rhizospheres of Five Plant Species-- Implications for Bioremediation of Contaminated Surface Soils written by and published by . This book was released on 1992 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this study was to collect data that would provide a foundation for the concept of using vegetation to enhance in situ bioremediation of contaminated surface soils. Soil and vegetation (Lespedeza cuneata, Paspalum notatum, Pinus taeda, and Solidago sp.) samples from the Miscellaneous Chemicals Basin (MCB) at the Savannah River Site were used in tests to identify critical plant and microbiological variables affecting the fate of trichloroethylene (TCE) in the root zone. Microbiological assays including phospholipid acid analyses, and 14C-acetate incorporation were conducted to elucidate differences in rhizosphere and nonvegetated soil microbial communities from the MCB. The microbial activity, biomass, and degradation of TCE in rhizosphere soils were significantly greater than corresponding nonvegetated soils. Vegetation had a positive effect on microbial degradation of 14C-TCE in whole-plant experiments. Soils from the MCB containing Lespedeza cuneata, Pinus taeda, and Glycine max mineralized greater than 25% of the 14C- TCE added compared with less than 20% in nonvegetated soils. Collectively, these results provide evidence for the positive role of vegetation in enhancing biodegradation.
Author : J. H. Wolfram
Publisher :
ISBN 13 :
Total Pages : 46 pages
Book Rating : 4.:/5 (227 download)
Download or read book The Microbial Degradation of TCE (Trichloroethylene). written by J. H. Wolfram and published by . This book was released on 1987 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this research was to characterize naturally occurring microorganisms capable of degrading trichloroethylene (TCE). Microorganisms tolerant to TCE were isolated from an aeration tower at Wurtsmith AFB MI and from industrial sludges at Hill AFB UT. Isolates from the Wurtsmith samples exhibited the highest density of cells with TCE as the only apparent organic carbon source and were selected as the inoculum for degradation studies. However, the capability of selected microorganisms to degrade TCE was not substantiated in both gas chromatography analyses and samples inoculated in culture bottles. Experiments using radiolabeled carbon-14 to study assimilation suggest that TCE is metabolized into a polar form by the microorganism. Subsequent cultures grown in radiolabeled TCE did not duplicate the previous results possible because of contamination, selection of variants or loss of culture. The organism responsible or the apparent TCE degradation may belong in the genus Citrobacter.
Author : Robert E. Hinchee
Publisher :
ISBN 13 :
Total Pages : 360 pages
Book Rating : 4.F/5 ( download)
Download or read book Bioremediation of Chlorinated Solvents written by Robert E. Hinchee and published by . This book was released on 1995 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soil and groundwater contamination stemming from the release of various chlorinated compounds into the environment is a significant and difficult site remediation challenge. The articles in this collection discuss the use of aerobic and anaerobic biological degradation to dehalogenate sites contaminated with pesticides and chlorinated solvents such as trichloroethylene, tetrachloroethene, tetrachloromethene, perchloroethylene, carbon tetrachloride, pentachlorophenol, and chlorinated benzene. Bench- and field-scale studies of the biological processes associated with in situ dechlorination of soil and aquifers are described. Discussed are the uses of microcosm studies and numerical simulation of dechlorination to manage system operation. Site characteristics (e.g., hydraulic properties, temperature, nitrogen availability) and their effect on the stability of the methanotrophic community are examined. Methods discussed include the use of air venting, alternative electron donors, biofilm reactors, surfactants, municipal digester sludge, iron enhancement, and sulfate reduction to improve conditions for the microbial consortia that effect dechlorination.
Author : Todd Alan Anderson
Publisher :
ISBN 13 :
Total Pages : 187 pages
Book Rating : 4.:/5 (132 download)
Download or read book Comparative Plant Uptake and Microbial Degradation of Trichloroethylene in the Rhizospheres of Five Plant Species written by Todd Alan Anderson and published by . This book was released on 1994 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt:
