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Methods Of Protein Microcharacterization
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Book Synopsis Methods of Protein Microcharacterization by : John E. Shively
Download or read book Methods of Protein Microcharacterization written by John E. Shively and published by Springer Science & Business Media. This book was released on 2008-02-22 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981
Book Synopsis Microcharacterization of Proteins by : Roland Kellner
Download or read book Microcharacterization of Proteins written by Roland Kellner and published by John Wiley & Sons. This book was released on 2008-07-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Both a thorough introduction and laboratory guide! This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing. The workshop was held in the Max Planck Institute for Biochemistry at Martinsried. It helped graduate students and researchers from academia and industry both to understand and apply themselves the most sophisticated methods available. This book offers a stimulating combination of basic concepts and practical applications - and is thus useful at all levels.
Book Synopsis Techniques in Protein Chemistry by :
Download or read book Techniques in Protein Chemistry written by and published by Academic Press. This book was released on 1996-05-23 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry VII, a valuable bench-top reference tool for protein chemists, features the most up-to-date advances in protein methodologies. Key Features * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Functional domain analysis * Protein design and engineering
Book Synopsis Proteins: Form and Function by : R.A. Bradshaw
Download or read book Proteins: Form and Function written by R.A. Bradshaw and published by Elsevier. This book was released on 2012-12-02 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are of fundamental importance in all aspects of cell structure and function. The study of proteins has always formed a central part of biochemistry, and recent expansion in the range and sophistication of available techniques has provided a wealth of new information. The current methods and approaches used to gain a better understanding of the structure of proteins are described within this book, while other articles focus on the role of proteins within the cell.Most of the articles have appeared previously in the monthly review journal Trends in Biochemical Sciences (TIBS), with a few commissioned specifically for this collection, which should appeal to students, lecturers and researchers interested in the form and function of proteins.
Book Synopsis A Practical Guide to Protein and Peptide Purification for Microsequencing by : Paul T. Matsudaira
Download or read book A Practical Guide to Protein and Peptide Purification for Microsequencing written by Paul T. Matsudaira and published by Elsevier. This book was released on 2012-12-02 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing
Book Synopsis Advances in Applied Microbiology by :
Download or read book Advances in Applied Microbiology written by and published by Academic Press. This book was released on 1991-07-15 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Applied Microbiology
Book Synopsis Synthetic Peptides by : Gregory Grant
Download or read book Synthetic Peptides written by Gregory Grant and published by Oxford University Press. This book was released on 2002-04-04 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first synthetic peptides were produced a century ago. In the ensuing period, they have developed as valuable research tools that are readily available to all researchers. However, since most researchers do not make their own peptides, they are often unfamiliar with not only the synthetic chemistry but also with important and useful aspects of design, analysis, handling, and applications. This volume is the second edition of a volume that was first published 10 years ago. It is written by experts in the field who provide detailed descriptions as well as practical advice for producing and using synthetic peptides. The various chapters cover peptide design considerations, the synthetic chemistry, the evaluation of the synthetic product, and the modern applications of synthetic peptides. This includes the basic principles of peptide structure, analysis and chain assembly as well as the latest in selective disulfide bond formation, new strategies for the production of large peptides, and sequencing peptides by mass spectrometry. This book was designed with the intent of providing useful information both for the novices to the field as well as more seasoned practitioners. Its contents will help prevent problems commonly encountered and allow scientists to optimize their use of synthetic peptides.
Book Synopsis Chemical Modification of Biological Polymers by : Roger L. Lundblad
Download or read book Chemical Modification of Biological Polymers written by Roger L. Lundblad and published by CRC Press. This book was released on 2016-04-19 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examining the chemical modification of biological polymers and the emerging applications of this technology, Chemical Modification of Biological Polymers reflects the change in emphasis in this subsection of biotechnology from the study of protein structure and function toward applications in therapeutics and diagnostics. HighlightsThe basic organi
Book Synopsis Protein Biotechnology by : Felix Franks
Download or read book Protein Biotechnology written by Felix Franks and published by Springer Science & Business Media. This book was released on 2008-02-22 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the servants of life. They occur in all com- nent parts of living organisms and are staggering in their fu- tional variety, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different p- teins, fulfilling a wide range of life-supporting roles. Additions to the total number of known proteins are being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation. The total international protein literature could fill a medi- sized building and is growing at an ever-increasing rate. The reader might be forgiven for asking whether yet another book on proteins, their properties, and functions can serve a useful purpose. An explanation of the origin of this book may serve as justification. The authors form the tutorial team for an int- sive postexperience course on protein characterization or- nized by the Center for Professional Advancement, East Brunswick, New Jersey, an educational foundation. The course was first mounted in Amsterdam in 1982 and has since been repeated several times, in both Amsterdam and the US, with participants from North America and most European countries. In a predecessor to this book, emphasis was placed on the role of protein isolation in the food industry, because at the time this reflected the interests of most of the participants at the course. Today, isolated proteins for food use are extracted from yeasts, fungal sources, legumes, oilseeds, cereals, and leaves.
Book Synopsis Fundamentals of Protein Biotechnology by : Stein
Download or read book Fundamentals of Protein Biotechnology written by Stein and published by Routledge. This book was released on 2017-10-19 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fills a gap between the existing studies of proteins, which tend to be highly technical and geared toward the practicing protein chemist, and biochemistry textbooks, which focus on general principles. Scientists cover a dozen topics by presenting fundamental principles, an overview, and the practica
Book Synopsis Amyloid, Prions, and Other Protein Aggregates by :
Download or read book Amyloid, Prions, and Other Protein Aggregates written by and published by Elsevier. This book was released on 1999-09-22 with total page 861 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Book Synopsis Characterization of Proteins by : Felix Franks
Download or read book Characterization of Proteins written by Felix Franks and published by Springer. This book was released on 2007-10-03 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the servants of life. They occur in all component parts of living organisms and are staggering in their functional var- ty, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different proteins, fulfilling a wide range of life-supporting roles. Their production is controlled by the cell’s genetic machinery, and a malfunction of even one protein in the cell will give rise to pathological symptoms. Additions to the total number of known proteins are constantly being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation; this latter technology has become known as protein engineering. The in vivo functioning of proteins depends critically on the chemical structure of individual peptide chains, but also on the detailed folding of the chains themselves and on their assembly into larger supramolecular structures. The molecules and their fu- tional assemblies possess a limited in vitro stability. Special methods are required for their intact isolation from the source material and for their analysis, both qualitatively and quantitatively. Proteins are also increasingly used as “industrial components,” e.g., in biosensors and immobilized enzymes, because of their specificity, selectivity, and sensitivity. This requires novel and refined proce- ing methods by which the protein isolate can be converted into a form in which it can be utilized.
Book Synopsis Techniques in Protein Modification by : Roger L. Lundblad
Download or read book Techniques in Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 1994-11-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.
Book Synopsis Chemical Reagents for Protein Modification by : Roger L. Lundblad
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2020-03-11 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
Book Synopsis Methods for Investigation of Amino Acid and Protein Metabolism by : Antoine E. El-Khoury
Download or read book Methods for Investigation of Amino Acid and Protein Metabolism written by Antoine E. El-Khoury and published by CRC Press. This book was released on 2017-10-05 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Containing all the new as well as classical methodologies used in the investigation of amino acid and protein metabolism in human and animal models, this book is needed because of the dramatic increase in research in this field. There is no other book currently on the market that covers these methods of investigation. Methods for Investigation of Amino Acid and Protein Metabolism explores areas such as amino acid transfer across tissue membranes, past and new applications using stable isotopes, protein synthesis in organs and tissues, and more. Because of the importance of research methods in the field of amino acid and protein nutrition and metabolism, this book facilitates the reader's integration of the concepts involved in these investigative research methods and their corollaries. In addition to helping any nutrition investigator design and conduct appropriate research protocols in this area of nutrition, this book assists students who are planning to investigate amino acid and protein metabolism in humans or laboratory animals.
Book Synopsis Laboratory Methodology in Biochemistry by : C. Fini
Download or read book Laboratory Methodology in Biochemistry written by C. Fini and published by CRC Press. This book was released on 2019-08-08 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry Physical Biochemistry Molecular Biology Clinical Biochemistry Biochemical Technology Applied Biochemistry Cellular Biology
Book Synopsis Capillary Electrophoresis in Analytical Biotechnology by : Pier Giorgio Righetti
Download or read book Capillary Electrophoresis in Analytical Biotechnology written by Pier Giorgio Righetti and published by CRC Press. This book was released on 1995-12-18 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new book on capillary electrophoresis (CE) is unique in its focus on biotechnology. It is devoted to proteins, peptides, and techniques especially useful in the area of recombinant DNA products. Emphasis is also placed on glycoproteins. Because of the growing role of the glycosylation process in CE, a comprehensive chapter on the subject acts as a book within a book. Although this well-known researcher in biotechnology presents a number of chapters extensively discussing theories, important practical aspects in the routine use of capillary electrophoresis are also covered.
