Methods Of Dna Extraction

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Molecular Microbial Diagnostic Methods

Author : Nigel Cook
Publisher : Academic Press
ISBN 13 : 0124171702
Total Pages : 268 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Molecular Microbial Diagnostic Methods by : Nigel Cook

Download or read book Molecular Microbial Diagnostic Methods written by Nigel Cook and published by Academic Press. This book was released on 2015-10-06 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Microbial Diagnostic Methods: Pathways to Implementation for the Food and Water Industry was developed by recognized and experienced highlevel scientists. It’s a comprehensive and detailed reference that uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. This book discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The content is also pertinent for anyone carrying out microbiological analysis at the research level, and for scientists developing methods, as it focuses on the requirements of end-users. Includes information on how to introduce and implement molecular methods for routine monitoring in food and water laboratories Discusses the importance of robust validation of molecular methods as alternatives to existing standard methods to help ensure the production of defendable results Highlights potential issues with respect to successful implementation of these methods

PCR Protocols

Author : John M. S. Bartlett
Publisher : Springer Science & Business Media
ISBN 13 : 1592593844
Total Pages : 1083 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis PCR Protocols by : John M. S. Bartlett

Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Biotechnologies for Plant Mutation Breeding

Author : Joanna Jankowicz-Cieslak
Publisher : Springer
ISBN 13 : 3319450212
Total Pages : 343 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Biotechnologies for Plant Mutation Breeding by : Joanna Jankowicz-Cieslak

Download or read book Biotechnologies for Plant Mutation Breeding written by Joanna Jankowicz-Cieslak and published by Springer. This book was released on 2016-12-08 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Plant Molecular Biology Manual

Author : Stanton Gelvin
Publisher : Springer Science & Business Media
ISBN 13 : 940115242X
Total Pages : 138 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Plant Molecular Biology Manual by : Stanton Gelvin

Download or read book Plant Molecular Biology Manual written by Stanton Gelvin and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fingerprinting Methods Based on Arbitrarily Primed PCR

Author : Maria R. Micheli
Publisher : Springer Science & Business Media
ISBN 13 : 3642604412
Total Pages : 435 pages
Book Rating : 4.6/5 (426 download)

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Book Synopsis Fingerprinting Methods Based on Arbitrarily Primed PCR by : Maria R. Micheli

Download or read book Fingerprinting Methods Based on Arbitrarily Primed PCR written by Maria R. Micheli and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes. The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.

Plant Genomes: Methods for Genetic and Physical Mapping

Author : J.S. Beckmann
Publisher : Springer Science & Business Media
ISBN 13 : 9401124426
Total Pages : 233 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Plant Genomes: Methods for Genetic and Physical Mapping by : J.S. Beckmann

Download or read book Plant Genomes: Methods for Genetic and Physical Mapping written by J.S. Beckmann and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: JACQUES S. BECKMANN & THOMAS C. OSBORN Extraordinary progress has been made in the analyses of the genetic structures of higher eukaryotic genomes. Only ten years elapsed between the initial proposals to use molecular DNA markers for the generation of a complete linkage map of the human genome [5, 17] and the first description of a 10 centimorgan map of one of its chromosomes [22], soon to be followed by others. The availability of molecular DNA markers, henceforth called genomic markers [for a review of their properties see 1, 2, 20], represents a milestone in genetics by providing the capacity for complete genetic coverage of all genomes. It is important to remember that the nature of the DNA polymorphism or of the specific method used to uncover it can be quite different for different marker loci. The genetic variation detected can be a result of a simple point mutation, a DNA insertion/deletion event, or a change in repeat copy number at some hypervariable DNA [11] or micro satellite [21] motif. Currently, the methods of detection can involve use of restriction endonucleases, nucleic acid hybridization, or DNA sequence amplification. Each of these sources of var iation and methods of detection can have utility for different applications. Furthermore, new approaches for the detection of DNA polymorphism are constantly emerging. The primary concern here is that the monitored poly morphism defines a genetic marker 'useful' for the desired application.

DNA Binding and DNA Extraction

Author : Chunxu Zhou
Publisher : Nova Science Publishers
ISBN 13 : 9781614709589
Total Pages : 0 pages
Book Rating : 4.7/5 (95 download)

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Book Synopsis DNA Binding and DNA Extraction by : Chunxu Zhou

Download or read book DNA Binding and DNA Extraction written by Chunxu Zhou and published by Nova Science Publishers. This book was released on 2012-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the authors present current research in the study of DNA binding and extraction methods, applications, and limitations. Topics discussed in this compilation include DNA binding to cationic polymers and its implications on gene therapy; metal based anticancer drug-DNA binding; non-covalent DNA-AuNP's interactions; prospects of nanoparticle DNA binding; extraction and purification of DNA from USAB reactor sludge samples; isolation of genomic DNA from microbes and microbial communities; dengue virus RNA extraction challenges and isolating bacterial and viral nucleic acids from clinical samples for diagnostic testing.

Low-Cost Methods for Molecular Characterization of Mutant Plants

Author : Bradley J. Till
Publisher : Springer
ISBN 13 : 3319162594
Total Pages : 42 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Low-Cost Methods for Molecular Characterization of Mutant Plants by : Bradley J. Till

Download or read book Low-Cost Methods for Molecular Characterization of Mutant Plants written by Bradley J. Till and published by Springer. This book was released on 2015-05-08 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

The Nucleic Acid Protocols Handbook

Author : Ralph Rapley
Publisher : Springer Science & Business Media
ISBN 13 : 1592590381
Total Pages : 997 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis The Nucleic Acid Protocols Handbook by : Ralph Rapley

Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

Techniques in Molecular Systematics and Evolution

Author : Rob DeSalle
Publisher : Birkhäuser
ISBN 13 : 3034881258
Total Pages : 414 pages
Book Rating : 4.0/5 (348 download)

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Book Synopsis Techniques in Molecular Systematics and Evolution by : Rob DeSalle

Download or read book Techniques in Molecular Systematics and Evolution written by Rob DeSalle and published by Birkhäuser. This book was released on 2013-12-01 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the

Advanced Techniques in Diagnostic Microbiology

Author : Yi-Wei Tang
Publisher : Springer Science & Business Media
ISBN 13 : 0387328920
Total Pages : 550 pages
Book Rating : 4.3/5 (873 download)

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Book Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang

Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer Science & Business Media. This book was released on 2007-01-16 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.

Molecular Techniques in Taxonomy

Author : Godfrey M. Hewitt
Publisher : Springer Science & Business Media
ISBN 13 : 3642839622
Total Pages : 404 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Molecular Techniques in Taxonomy by : Godfrey M. Hewitt

Download or read book Molecular Techniques in Taxonomy written by Godfrey M. Hewitt and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.

Basic Techniques in Molecular Biology

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Basic Techniques in Molecular Biology by : Stefan Surzycki

Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Molecular Tools for Screening Biodiversity

Author : A. Karp
Publisher : Springer Science & Business Media
ISBN 13 : 9400900198
Total Pages : 482 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Molecular Tools for Screening Biodiversity by : A. Karp

Download or read book Molecular Tools for Screening Biodiversity written by A. Karp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mark Chase There are many literature resources available to molecular biologists wishing to assess genetic variation, but the myriad of techniques and approaches potentially available to the plant breeder and the evolutionary biologist is truly bewildering, and most have never been evaluated side-by-side on the same sets of samples. Additionally, it is often not recognized that tools that are useful for breeders can often be adapted for use in evolutionary studies and vice versa, but this is generally the case. The borderline between population genetics and phylogenetics is vague and difficult to assess, and a combination of both types of tools is best when it is not clear with which area one is dealing. Furthermore, it is not now appropriate to use just one type of marker in any kind of study; most markers have the potential to misinform under certain conditions, so it is always wise to incorporate at least two different types of assessments into any project. This volume is designed to facilitate this sort of multiple approach and provides comparative data on most currently available methods so that researchers can more intelligently select those appropriate to their area of interest, regardless of whether it is in the realm of breeding or evolutionary biology.

4th International Conference on Biomedical Engineering in Vietnam

Author : Vo Van Toi
Publisher : Springer Science & Business Media
ISBN 13 : 3642321828
Total Pages : 424 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis 4th International Conference on Biomedical Engineering in Vietnam by : Vo Van Toi

Download or read book 4th International Conference on Biomedical Engineering in Vietnam written by Vo Van Toi and published by Springer Science & Business Media. This book was released on 2012-09-21 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the proceedings of the Fourth International Conference on the Development of Biomedical Engineering in Vietnam which was held in Ho Chi Minh City as a Mega-conference. It is kicked off by the Regenerative Medicine Conference with the theme “BUILDING A FACE” USING A REGENERATIVE MEDICINE APPROACH”, endorsed mainly by the Tissue Engineering and Regenerative Medicine International Society (TERMIS). It is followed by the Computational Medicine Conference, endorsed mainly by the Computational Surgery International Network (COSINE) and the Computational Molecular Medicine of German National Funding Agency; and the General Biomedical Engineering Conference, endorsed mainly by the International Federation for Medical and Biological Engineering (IFMBE). It featured the contributions of 435 scientists from 30 countries, including: Australia, Austria, Belgium, Canada, China, Finland, France, Germany, Hungary, India, Iran, Italy, Japan, Jordan, Korea, Malaysia, Netherlands, Pakistan, Poland, Russian Federation, Singapore, Spain, Switzerland, Taiwan, Turkey, Ukraine, United Kingdom, United States, Uruguay and Viet Nam.

Current Protocols in Molecular Biology

Author :
Publisher :
ISBN 13 : 9780471503385
Total Pages : pages
Book Rating : 4.5/5 (33 download)

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Book Synopsis Current Protocols in Molecular Biology by :

Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Polymerase Chain Reaction

Author : Kary B. Mullis
Publisher : Springer Science & Business Media
ISBN 13 : 1461202574
Total Pages : 464 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis The Polymerase Chain Reaction by : Kary B. Mullis

Download or read book The Polymerase Chain Reaction written by Kary B. Mullis and published by Springer Science & Business Media. This book was released on 2012-02-02 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...