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Methods In Molecular Biology Free Radical And Antioxidant Protocols
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Book Synopsis Free Radicals and Antioxidant Protocols by : Rao M. Uppu
Download or read book Free Radicals and Antioxidant Protocols written by Rao M. Uppu and published by Humana Press. This book was released on 2012-04-06 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers.
Book Synopsis Methods in Molecular Biology: Free radical and antioxidant protocols by : John M. Walker
Download or read book Methods in Molecular Biology: Free radical and antioxidant protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Oxidants and Antioxidants by : Donald Armstrong
Download or read book Oxidants and Antioxidants written by Donald Armstrong and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with “oxidative stress” using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Western blotting are the most widely used. Several forms of therapy are now available for patients with increased oxidative stress. In addition to standard antioxidant therapy supplementation in vivo and in vitro, photodynamic therapy (PDT) employs excitation of a photon-emitting compound delivered systemically for free radical-mediated necrosis of affected tissues, and stem cells are also being used to induce signaling events or replace antioxidant enzymes.
Book Synopsis Oxidative Stress Biomarkers and Antioxidant Protocols by : Donald Armstrong
Download or read book Oxidative Stress Biomarkers and Antioxidant Protocols written by Donald Armstrong and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first protocols book, Free Radical and Antioxidant Protocols (1) was published in late 1998. Sections were divided into three parts, covering selected biochemical techniques for measuring oxidative stress, antioxidant (AOX) activity, and combined applications. In choosing the 40 methods to be included in that book, I realized there were considerably more of equal value than that which we could have presented in a single volume. To produce a comprehensive resource, this book and a third are being compiled to expand coverage of the field. A summary of papers (2) published on this important subject emphasizes the continuing rapid growth in oxidative stress investigations relating to our understanding of biochemical reactions, their relevance to pathophysiological mechanisms, how disease may arise, and how therapeutic intervention may be achieved(3). Although there is some overlap between the categories, the ana- sis shown below illustrates where current studies are concentrated and are almost evenly distributed between free radicals and AOX. Over the last 4 yr, there has been a 55% increase in the number of papers published in the area.
Book Synopsis Free Radical and Antioxidant Protocols by : Donald Armstrong
Download or read book Free Radical and Antioxidant Protocols written by Donald Armstrong and published by Springer. This book was released on 2010-10-28 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents an outstanding collection of state-of-the-art methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation and technology-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. The laboratory-tested free-radical assays described here in detail will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
Book Synopsis Advanced Protocols in Oxidative Stress I by : Donald Armstrong
Download or read book Advanced Protocols in Oxidative Stress I written by Donald Armstrong and published by Humana Press. This book was released on 2008-10-09 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Due to the dynamic nature of this topic, this book will be the first of several volumes of Advanced Protocols in Oxidative Stress, all part of the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters include a brief introduction to the material, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and ensuring replication of technology. Cutting-edge and convenient, Advanced Protocols in Oxidative Stress I is an ideal desk reference for scientists wishing to further this research in this exciting, unique and vital field of study.
Book Synopsis Techniques in Free Radical Research by : A.T. Diplock
Download or read book Techniques in Free Radical Research written by A.T. Diplock and published by Elsevier. This book was released on 1991-12-11 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage. Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
Book Synopsis Advanced Protocols in Oxidative Stress I by : D. Armstrong
Download or read book Advanced Protocols in Oxidative Stress I written by D. Armstrong and published by Methods in Molecular Biology. This book was released on 2008-10-09 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods.
Book Synopsis Methods in Molecular Biology: Oxidative stress biomarkers and antioxidant protocols by : John M. Walker
Download or read book Methods in Molecular Biology: Oxidative stress biomarkers and antioxidant protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Oxidative Stress and Antioxidant Protection by : Donald Armstrong
Download or read book Oxidative Stress and Antioxidant Protection written by Donald Armstrong and published by John Wiley & Sons. This book was released on 2016-04-11 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oxidative Stress and Antioxidant Protection: The Science of Free Radical Biology and Disease Oxidative Stress and Antioxidant Protection begins with a historical perspective of pioneers in oxidative stress with an introductory section that explains the basic principles related to oxidative stress in biochemistry and molecular biology, demonstrating both pathways and biomarkers. This section also covers diagnostic imaging and differential diagnostics. The following section covers psychological, physiologic, pharmacologic and pathologic correlates. This section addresses inheritance, gender, nutrition, obesity, family history, behavior modification, natural herbal-botanical products, and supplementation in the treatment of disease. Clinical trials are also summarized for major medical disorders and efficacy of treatment, with particular focus on inflammation, immune response, recycling, disease progression, outcomes and interventions. Each of the chapters describes what biomarker(s) and physiological functions may be relevant to a concept of specific disease and potential alternative therapy. The chapters cover medical terminology, developmental change, effects of aging, senescence, lifespan, and wound healing, and also illustrates cross-over exposure to other fields. The final chapter covers how and when to interpret appropriate data used in entry level biostatistics and epidemiology. Authored and edited by leaders in the field, Oxidative Stress and Antioxidant Protection will be an invaluable resource for students and researchers studying cell biology, molecular biology, and biochemistry, as well professionals in various health science fields.
Book Synopsis Methods in Molecular Biology: Oxidants and antioxidants: ultrastructure and molecular biology protocols by : John M. Walker
Download or read book Methods in Molecular Biology: Oxidants and antioxidants: ultrastructure and molecular biology protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Protocols in Oxidative Stress III by : Donald Armstrong
Download or read book Advanced Protocols in Oxidative Stress III written by Donald Armstrong and published by Humana. This book was released on 2016-09-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.
Book Synopsis Free Radical and Antioxidant Protocols by : Donald Armstrong, 3rd
Download or read book Free Radical and Antioxidant Protocols written by Donald Armstrong, 3rd and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents an outstanding collection of state-of-the-art methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation and technology-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. The laboratory-tested free-radical assays described here in detail will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
Book Synopsis Advanced Protocols in Oxidative Stress II by : Donald Armstrong
Download or read book Advanced Protocols in Oxidative Stress II written by Donald Armstrong and published by Humana. This book was released on 2016-08-23 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.
Book Synopsis Free Radicals, Antioxidants and Diseases by : Rizwan Ahmad
Download or read book Free Radicals, Antioxidants and Diseases written by Rizwan Ahmad and published by BoD – Books on Demand. This book was released on 2018-08-01 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: The current book entitled Free Radicals, Antioxidants, and Diseases gives an idea of detecting free radicals in vivo by newer techniques and provides insights into the roles played by various antioxidants in combating diseases caused by oxidative stress. The chapters included in this volume showcase new investigation in this field by the research groups around the world.
Book Synopsis Advanced Protocols in Oxidative Stress by :
Download or read book Advanced Protocols in Oxidative Stress written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Free Radicals in Biology and Medicine by : Barry Halliwell
Download or read book Free Radicals in Biology and Medicine written by Barry Halliwell and published by OUP Oxford. This book was released on 2015-08-21 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free Radicals in Biology and Medicine has become a classic text in the field of free radical and antioxidant research. Now in its fifth edition, the book has been comprehensively rewritten and updated whilst maintaining the clarity of its predecessors. Two new chapters discuss 'in vivo' and 'dietary' antioxidants, the first emphasising the role of peroxiredoxins and integrated defence mechanisms which allow useful roles for ROS, and the second containing new information on the role of fruits, vegetables, and vitamins in health and disease. This new edition also contains expanded coverage of the mechanisms of oxidative damage to lipids, DNA, and proteins (and the repair of such damage), and the roles played by reactive species in signal transduction, cell survival, death, human reproduction, defence mechanisms of animals and plants against pathogens, and other important biological events. The methodologies available to measure reactive species and oxidative damage (and their potential pitfalls) have been fully updated, as have the topics of phagocyte ROS production, NADPH oxidase enzymes, and toxicology. There is a detailed and critical evaluation of the role of free radicals and other reactive species in human diseases, especially cancer, cardiovascular, chronic inflammatory and neurodegenerative diseases. New aspects of ageing are discussed in the context of the free radical theory of ageing. This book is recommended as a comprehensive introduction to the field for students, educators, clinicians, and researchers. It will also be an invaluable companion to all those interested in the role of free radicals in the life and biomedical sciences.
