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Author : So Iwata
Publisher : Internat'l University Line
ISBN 13 : 9780963681799
Total Pages : 392 pages
Book Rating : 4.6/5 (817 download)
Download or read book Methods and Results in Crystallization of Membrane Proteins written by So Iwata and published by Internat'l University Line. This book was released on 2003 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Isabel Moraes
Publisher : Springer
ISBN 13 : 3319350722
Total Pages : 184 pages
Book Rating : 4.3/5 (193 download)
Download or read book The Next Generation in Membrane Protein Structure Determination written by Isabel Moraes and published by Springer. This book was released on 2016-08-23 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.
Author : Hartmut Michel
Publisher : CRC Press
ISBN 13 : 1351088173
Total Pages : 376 pages
Book Rating : 4.3/5 (51 download)
Download or read book Crystallization of Membrane Proteins written by Hartmut Michel and published by CRC Press. This book was released on 2018-01-18 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The precise knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action. It also lays the foundation for rational protein and drug design. The only method to obtain this knowledge is still crystallography. At present, the structures of about 400 proteins are known at or nearly at atomic proteins. However, only two of them are membrane proteins or complexes of the membrane proteins. The reasons for the difference is not the crystals of membrane proteins resists forming special problems when being analysed. The reason is that the membrane proteins resist into forming into well-ordered crystals. The intention of this book is to help to produce well-ordered crystals proteins and to provide guidelines, it is aimed at both biochemists and protein crystallographer‘s.
Author : Terese M. Bergfors
Publisher : Internat'l University Line
ISBN 13 : 0972077448
Total Pages : 505 pages
Book Rating : 4.9/5 (72 download)
Download or read book Protein Crystallization written by Terese M. Bergfors and published by Internat'l University Line. This book was released on 2009 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Academic Press
ISBN 13 : 9780080961590
Total Pages : 336 pages
Book Rating : 4.9/5 (615 download)
Download or read book Membrane Protein Crystallization written by and published by Academic Press. This book was released on 2009-05-29 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.
Author : Carola Hunte
Publisher : Elsevier
ISBN 13 : 0080536174
Total Pages : 316 pages
Book Rating : 4.0/5 (85 download)
Download or read book Membrane Protein Purification and Crystallization written by Carola Hunte and published by Elsevier. This book was released on 2003-01-05 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins
Author : Alexander Wlodawer
Publisher : Humana Press
ISBN 13 : 9781493969982
Total Pages : 672 pages
Book Rating : 4.9/5 (699 download)
Download or read book Protein Crystallography written by Alexander Wlodawer and published by Humana Press. This book was released on 2017-06-02 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.
Author : Arnaud Ducruix
Publisher :
ISBN 13 : 0199636788
Total Pages : 460 pages
Book Rating : 4.1/5 (996 download)
Download or read book Crystallization of Nucleic Acids and Proteins written by Arnaud Ducruix and published by . This book was released on 1999 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucleic Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucleic Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.
Author : Sukarno Ferreira
Publisher : BoD – Books on Demand
ISBN 13 : 9535110101
Total Pages : 436 pages
Book Rating : 4.5/5 (351 download)
Download or read book Advanced Topics on Crystal Growth written by Sukarno Ferreira and published by BoD – Books on Demand. This book was released on 2013-02-20 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crystal growth is the key step of a great number of very important applications. The development of new devices and products, from the traditional microelectronic industry to pharmaceutical industry and many others, depends on crystallization processes. The objective of this book is not to cover all areas of crystal growth but just present, as specified in the title, important selected topics, as applied to organic and inorganic systems. All authors have been selected for being key researchers in their field of specialization, working in important universities and research labs around the world. The first section is mainly devoted to biological systems and covers topics like proteins, bone and ice crystallization. The second section brings some applications to inorganic systems and describes more general growth techniques like chemical vapor crystallization and electrodeposition. This book is mostly recommended for students working in the field of crystal growth and for scientists and engineers in the fields of crystalline materials, crystal engineering and the industrial applications of crystallization processes.
Author : Jens Dietrich
Publisher : Imperial College Press
ISBN 13 : 186094678X
Total Pages : 154 pages
Book Rating : 4.8/5 (69 download)
Download or read book Strategies for Two-dimensional Crystallization of Proteins Using Lipid Monolayers written by Jens Dietrich and published by Imperial College Press. This book was released on 2005 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Strategies for Two-Dimensional Crystallization of Proteins UsingLipid Monolayers presents an overview of different methods thatlead to structure determination by electron microscopy. These methodshave proven to be extremely successful, especially for elucidating thestructure of membrane proteins.
Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
ISBN 13 : 159259400X
Total Pages : 330 pages
Book Rating : 4.5/5 (925 download)
Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Author : Ales Iglic
Publisher : Academic Press
ISBN 13 : 9780123812674
Total Pages : 296 pages
Book Rating : 4.8/5 (126 download)
Download or read book Advances in Planar Lipid Bilayers and Liposomes written by Ales Iglic and published by Academic Press. This book was released on 2010-11-03 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Planar Lipid Bilayers and Liposomes, Volume 9, continues to include invited chapters on a broad range of topics, covering both main arrangements of the reconstituted system, namely planar lipid bilayers and spherical liposomes. The invited authors present the latest results in this exciting multidisciplinary field of their own research group. Many of the contributors working in both fields over many decades were in close collaboration with the late Prof. H. Ti Tien, the founding editor of this book series. There are also chapters written by some of the younger generation of scientists included in this series. This volume keeps in mind the broader goal with both systems, planar lipid bilayers and spherical liposomes, which is the further development of this interdisciplinary field worldwide. Incorporates contributions from newcomers and established and experienced researchers Explores the planar lipid bilayer systems and spherical liposomes from both theoretical and experimental perspectives Serves as an indispensable source of information for new scientists
Author : Jean-Jacques Lacapere
Publisher : Humana Press
ISBN 13 : 9781493971497
Total Pages : 419 pages
Book Rating : 4.9/5 (714 download)
Download or read book Membrane Protein Structure and Function Characterization written by Jean-Jacques Lacapere and published by Humana Press. This book was released on 2017-08-10 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author : Jaime Gómez Morales
Publisher : MDPI
ISBN 13 : 3039214039
Total Pages : 184 pages
Book Rating : 4.0/5 (392 download)
Download or read book Biological Crystallization written by Jaime Gómez Morales and published by MDPI. This book was released on 2019-09-23 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: For at least six hundred million years, life has been a fascinating laboratory of crystallization, referred to as biomineralization. During this huge lapse of time, many organisms from diverse phyla have developed the capability to precipitate various types of minerals, exploring distinctive pathways for building sophisticated structural architectures for different purposes. The Darwinian exploration was performed by trial and error, but the success in terms of complexity and efficiency is evident. Understanding the strategies that those organisms employ for regulating the nucleation, growth, and assembly of nanocrystals to build these sophisticated devices is an intellectual challenge and a source of inspiration in fields as diverse as materials science, nanotechnology, and biomedicine. However, “Biological Crystallization” is a broader topic that includes biomineralization, but also the laboratory crystallization of biological compounds such as macromolecules, carbohydrates, or lipids, and the synthesis and fabrication of biomimetic materials by different routes. This Special Issue collects 15 contributions ranging from biological and biomimetic crystallization of calcium carbonate, calcium phosphate, and silica-carbonate self-assembled materials to the crystallization of biological macromolecules. Special attention has been paid to the fundamental phenomena of crystallization (nucleation and growth), and the applications of the crystals in biomedicine, environment, and materials science.
Author : Terese M. Bergfors
Publisher : International Univ Line
ISBN 13 : 9780963681751
Total Pages : 306 pages
Book Rating : 4.6/5 (817 download)
Download or read book Protein Crystallization written by Terese M. Bergfors and published by International Univ Line. This book was released on 1999 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Naomi E. Chayen
Publisher : Internat'l University Line
ISBN 13 : 9780972077439
Total Pages : 300 pages
Book Rating : 4.0/5 (774 download)
Download or read book Protein Crystallization Strategies for Structural Genomics written by Naomi E. Chayen and published by Internat'l University Line. This book was released on 2007 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mark R. Sanderson
Publisher : OUP Oxford
ISBN 13 : 0191523429
Total Pages : 304 pages
Book Rating : 4.1/5 (915 download)
Download or read book Macromolecular Crystallography written by Mark R. Sanderson and published by OUP Oxford. This book was released on 2007-08-23 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of accurate molecular structures is a pre-requisite for rational drug design, and for structure-based function studies to aid the development of effective therapeutic agents and drugs. The successful determination of the complete genome (genetic sequence) of several species (including humans) has recently directed scientific attention towards identifying the structure and function of the complete complement of proteins that make up that species; a new and rapidly growing field of study called 'structural genomics'. There are now several important and well-funded global initiatives in operation to identify all of the proteins of key model species. One of the main requirements for these initiatives is a high-throughput crystallization facility to speed-up the protein identification process. The extent to which these technologies have advanced, calls for an updated review of current crystallographic theory and practice. This practical reference book features the latest conventional and high-throughput methods, and includes contributions from a team of internationally recognized leaders and experts. It will be of relevance and use to graduate students, research scientists and professionals currently working in the field of conventional and high-throughput macromolecular crystallography.
