Author : Yuan Liu (Ph.D.)
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)
Book Synopsis Method Development and Application of Mass Spectrometry for Better Understanding of Disease Mechanisms and Diagnosis by : Yuan Liu (Ph.D.)
Download or read book Method Development and Application of Mass Spectrometry for Better Understanding of Disease Mechanisms and Diagnosis written by Yuan Liu (Ph.D.) and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry is a very powerful tool for identifying and quantifying biomolecules of interest, including metabolites, drug compounds, proteins, and post-translational modifications of (PTMs) of proteins. In mass spectrometry (MS)-based analytical techniques, liquid-chromatography coupled with tandem mass spectrometry (LC-MS/MS) is widely used for biomolecule analysis, including relative and absolute quantification of metabolites, proteins and PTMs. Data dependent acquisition (DDA) and data-independent acquisition (DIA) are two main methods for quantification of biomolecules in LC-MS/MS. By integrating isotopic and/or isobaric tags labeling, the DDA method enables precise absolute quantification and high-throughput analysis of biomolecules, facilitating biomarker discovery and elucidation of disease mechanisms. In contrast, DIA has garnered significant interest due to its exceptional reproducibility and depth in identifying and quantifying biomolecules. In this dissertation work, I apply these isotopic/isobaric tag labeling and label-free strategies to investigate potential biomarkers in the serum of Alzheimer's disease (AD) and colorectal cancer (CRC). Additionally, I explore the crosstalk between pancreatic stellate cells and pancreatic cancer cells using multi-faceted MS approaches.In addition to LC-MS/MS, mass spectrometry imaging (MSI) offers unparalleled insights into the spatial distribution of biomolecules across organs, tissues, and cell cultures, further enhancing our understanding of biological systems. We develop a tagging method to enhance the identification, quantification, and visualization of amine-containing metabolites by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Furthermore, I optimize a workflow for rapid sample preparation and high throughput MSI of biomolecule distribution in a widely used three-dimensional cell culture system - spheroids. In summary, the integration of various mass spectrometry techniques and method development discussed in this dissertation demonstrates that combining different mass spectrometry approaches can provide a more comprehensive molecular picture of the complex biological system, ultimately enhancing our understanding of disease mechanisms and advancing diagnosis and treatment options.