Mass Spectrometry Of Glycoproteins

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Mass Spectrometry of Glycoproteins

Author : Arnaud Delobel
Publisher : Humana
ISBN 13 : 9781071612408
Total Pages : 403 pages
Book Rating : 4.6/5 (124 download)

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Book Synopsis Mass Spectrometry of Glycoproteins by : Arnaud Delobel

Download or read book Mass Spectrometry of Glycoproteins written by Arnaud Delobel and published by Humana. This book was released on 2021-07-04 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.

Mass Spectrometry of Glycoproteins

Author : Jennifer J. Kohler
Publisher : Humana Press
ISBN 13 : 9781493962037
Total Pages : 352 pages
Book Rating : 4.9/5 (62 download)

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Book Synopsis Mass Spectrometry of Glycoproteins by : Jennifer J. Kohler

Download or read book Mass Spectrometry of Glycoproteins written by Jennifer J. Kohler and published by Humana Press. This book was released on 2016-05-01 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive new resource in Springer s Methods in Molecular Biology series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry."

Mass Spectrometry–Based Glycoproteomics and Its Clinic Application

Author : Haojie Lu
Publisher : CRC Press
ISBN 13 : 100040661X
Total Pages : 216 pages
Book Rating : 4.0/5 (4 download)

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Book Synopsis Mass Spectrometry–Based Glycoproteomics and Its Clinic Application by : Haojie Lu

Download or read book Mass Spectrometry–Based Glycoproteomics and Its Clinic Application written by Haojie Lu and published by CRC Press. This book was released on 2021-09-20 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: As one of the most extensive and important protein post-translational modifications, glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently, researchers have focused on the need to characterize protein glycosylation sites, structures, and their degree of modification, to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative, quantitative, and data analysis, together with their clinical applications. Significant examples are discussed to illustrate the principles, laboratory protocols, and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine. Editor: Haojie Lu is a professor at Fudan University, specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.

Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry

Download Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry PDF Online Free

Author : Ganglong Yang
Publisher : Frontiers Media SA
ISBN 13 : 2889746704
Total Pages : 231 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry by : Ganglong Yang

Download or read book Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry written by Ganglong Yang and published by Frontiers Media SA. This book was released on 2022-03-14 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mass Spectrometry-Based Chemical Proteomics

Author : W. Andy Tao
Publisher : John Wiley & Sons
ISBN 13 : 1118970217
Total Pages : 448 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao

Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Essentials of Glycobiology

Author : Ajit Varki
Publisher : CSHL Press
ISBN 13 : 9780879696818
Total Pages : 694 pages
Book Rating : 4.6/5 (968 download)

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Book Synopsis Essentials of Glycobiology by : Ajit Varki

Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.

Mass Spectrometry

Author : Michael A. Madson
Publisher : Elsevier
ISBN 13 : 0128041358
Total Pages : 88 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Mass Spectrometry by : Michael A. Madson

Download or read book Mass Spectrometry written by Michael A. Madson and published by Elsevier. This book was released on 2016-05-06 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules. As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study. Authored by an analytical chemist with more than 30 years of experience in research and industry Serves as a quick reference in mass spectral analysis and carbohydrates Includes more than 60 figures to aid in the retention of key concepts

Functional and Structural Proteomics of Glycoproteins

Author : Raymond J. Owens
Publisher : Springer Science & Business Media
ISBN 13 : 9048193559
Total Pages : 189 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis Functional and Structural Proteomics of Glycoproteins by : Raymond J. Owens

Download or read book Functional and Structural Proteomics of Glycoproteins written by Raymond J. Owens and published by Springer Science & Business Media. This book was released on 2010-12-02 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been predicted that nearly half of all human proteins are glycosylated indicating the significance of glycoproteins in human health and disease. For example, the glycans attached to proteins have emerged as important biomarkers in the diagnosis of diseases such as cancers and play a significant role in how pathogenic viruses gain entry into human cells. The study of glycoproteins has now become a truly proteomic science. In the last few years, technology developments including in silico methods, high throughput separation and detection techniques have accelerated the characterization of glycoproteins in cells and tissues. Glyco-engineering coupled to rapid recombinant protein production has facilitated the determination of glycoprotein structures key to exploring and exploiting their functional roles. Each chapter in this volume is written by experts in the field and together provide a review of the state of the art in the emerging field of glycoproteomics.

Recombinant Glycoproteins

Author : Steven B. Bradfute
Publisher : Springer Nature
ISBN 13 : 1071636669
Total Pages : 357 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Recombinant Glycoproteins by : Steven B. Bradfute

Download or read book Recombinant Glycoproteins written by Steven B. Bradfute and published by Springer Nature. This book was released on 2024 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zusammenfassung: This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins

Mass Spectrometric Analysis of Selected Glycoproteins

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (681 download)

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Book Synopsis Mass Spectrometric Analysis of Selected Glycoproteins by :

Download or read book Mass Spectrometric Analysis of Selected Glycoproteins written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: (Uncorrected OCR) Abstract of thesis entitled MASS SPECTROMETRIC ANALYSIS OF SELECTED GLYCOPROTEINS Submitted by Chan Chun Yu for the degree of Master of Philosophy at The University of Hong Kong in February 2005 Fetuin from fetal calf serum (Sigma, F6131), a well-characterized glycoprotein that has a molecular weight of 48.4 kDa and 3 N-glycosylation sites, was used as a prototypical protein to develop mass spectrometric approaches toward the identification and sequence elucidation of the N-glycosylation sites of proteins. Fetuin was digested using trypsin (porcine) and deglycosylated using peptide N-glycosidase F (PNGase-F) prior to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometric analysis. For peptide mass fingerprinting, the experimental peak list obtained from the MALDI-TOF mass spectrum of the sample was compared with the theoretical peak list obtained from the National Center for Biotechnology Information sequence database. The identification of each N-glycosylation site was based on the 0.98-Da mass increase observed upon hydrolysis by the amidase PNGase-F of the glycosylamine linkage of each N-glycosylated asparagine, which formed an aspartic acid residue. The identities of the glycopeptides were verified through isotopic pattern analysis using the proposed amino acid composition and the ISOPRO shareware program. All the 3 potential N-glycosylation sites of fetuin were identified successfully in accordance with published results. The peptide sequence coverage was 75%. The developed strategy was then applied to map the N-glycosylation sites of the severe acute respiratory syndrome spike S glycoprotein, a novel glycoprotein that requires a more complex analysis for its total 23 potential N-glycosylation sites. The proposed peptide sequence and its potential N-glycosylation sites were elucidated using tandem mass spectrometry under low-energy collision-induced dissociation. The partial peptide sequence was determined by.

Glycoprotein Analysis

Author : Weston B Struwe
Publisher : Royal Society of Chemistry
ISBN 13 : 1839166444
Total Pages : 0 pages
Book Rating : 4.8/5 (391 download)

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Book Synopsis Glycoprotein Analysis by : Weston B Struwe

Download or read book Glycoprotein Analysis written by Weston B Struwe and published by Royal Society of Chemistry. This book was released on 2024-10-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glycoproteins are central to numerous cellular processes and are among the most structurally complex biomolecules in nature. This unique complexity stems from variability in complex oligosaccharides that are located throughout the protein, a feature that is profoundly important for regulating biomolecular interactions but also makes glycoproteins difficult to study. As such, glycoprotein analysis entails a range of techniques to bridge the knowledge gap between glycoprotein structure and biological function. This book serves as an authoritative guide to glycoprotein analysis, written by internationally recognised experts in the field and discussed in the context of real-world applications across the life sciences. It provides a wide-ranging assessment of the modern methods, from those used to characterise glycoprotein structure, to approaches proficient in uncovering the molecular mechanisms by which they function as well as those capable of measuring structural dynamics and macromolecular assembly. These methods differ to a large extent and include mass spectrometry, glycan/lectin arrays, nuclear magnetic resonance, infrared spectroscopy, scanning probe microscopy and high-performance liquid chromatography. Equally important are computational techniques, including molecular dynamics and bioinformatics, which are also covered and discussed in the wider context of glycoprotein analysis. Glycobiology is indeed a rapidly growing field and the development of advanced tools for glycoproteins analysis has been enabled by researchers from different backgrounds working to overcome long-standing analytical challenges and biological questions involving glycosylation. This book is intended to aid academic and professional researchers at various levels of their career to gain a deeper appreciation of cutting-edge methods in glycoprotein analysis and their applications in biomolecular research, biotherapeutic development, structural biology and biophysical chemistry.

Handbook of Glycomics

Author : Richard D. Cummings
Publisher : Academic Press
ISBN 13 : 0080920055
Total Pages : 507 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Handbook of Glycomics by : Richard D. Cummings

Download or read book Handbook of Glycomics written by Richard D. Cummings and published by Academic Press. This book was released on 2009-10-19 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Glycomics provides the first comprehensive overview of the emerging field of glycomics, defined as the study of all complex carbohydrates in an organism or cell ("the glycome"). Beginning with analytic approaches and bioinformatics, this work provides a detailed discussion of relevant databases, data integration, and analysis. It then moves on to a discussion of specific model organism and pathogen glycomes followed by therapeutic approaches to human disorders of glycosylization. Structure and function of glycomes are included along with state-of-the-art technologies and systems approaches to the analysis of glycans. Synthesizes contributions from experts in biology, chemistry, bioinformatics, biotechnology, and medicine Highlights chapters devoted to chemical synthesis, cancer glycomics and immune cell glycomics Includes discussions of proteomics, mass spectrometry, NMR, array technology, and transcriptomics analytic approaches

Transforming Glycoscience

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309260868
Total Pages : 171 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis Transforming Glycoscience by : National Research Council

Download or read book Transforming Glycoscience written by National Research Council and published by National Academies Press. This book was released on 2012-10-23 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new focus on glycoscience, a field that explores the structures and functions of sugars, promises great advances in areas as diverse as medicine, energy generation, and materials science, this report finds. Glycans-also known as carbohydrates, saccharides, or simply as sugars-play central roles in many biological processes and have properties useful in an array of applications. However, glycans have received little attention from the research community due to a lack of tools to probe their often complex structures and properties. Transforming Glycoscience: A Roadmap for the Future presents a roadmap for transforming glycoscience from a field dominated by specialists to a widely studied and integrated discipline, which could lead to a more complete understanding of glycans and help solve key challenges in diverse fields.

The Glycome

Author : Adeel Malik
Publisher : CRC Press
ISBN 13 : 1000344320
Total Pages : 305 pages
Book Rating : 4.0/5 (3 download)

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Book Synopsis The Glycome by : Adeel Malik

Download or read book The Glycome written by Adeel Malik and published by CRC Press. This book was released on 2021-07-07 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive understanding of the enigmatic identity of the glycome, a complex but important area of research that has been largely ignored due to its complexity. The authors thoroughly deal with almost all aspects of the glycome, i.e., elucidation of the glycan identity enigma and its role in regulation of the cellular process, and in disease etiology. The book bridges the knowledge gap in understanding the glycome, from being a cell signature to its applications in disease etiology. In addition, it details many of the major insights regarding the possible role of the glycome in various diseases as a therapeutic marker. The book systematically covers the major aspects of the glycome, including the significance of substituting the diverse monosaccharide units to glycoproteins, the role of glycans in disease pathologies, and the challenges and advances in glycobiology. The authors stress the significance and huge encoding power of carbohydrates as well as provide helpful insights in framing the bigger picture. The Glycome: Understanding the Diversity and Complexity of Glycobiology details state-of-the-art developments and emerging challenges of glycome biology, which are going to be key areas of future research, not only in the glycobiology field but also in pharmaceutics.

Structure Determination of Glycoproteins by Mass Spectrometry

Author : Chenhui Zeng
Publisher :
ISBN 13 :
Total Pages : 231 pages
Book Rating : 4.:/5 (353 download)

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Book Synopsis Structure Determination of Glycoproteins by Mass Spectrometry by : Chenhui Zeng

Download or read book Structure Determination of Glycoproteins by Mass Spectrometry written by Chenhui Zeng and published by . This book was released on 1996 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Glycobiophysics

Author : Yoshiki Yamaguchi
Publisher : Springer
ISBN 13 : 9811321582
Total Pages : 278 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Glycobiophysics by : Yoshiki Yamaguchi

Download or read book Glycobiophysics written by Yoshiki Yamaguchi and published by Springer. This book was released on 2018-11-27 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents state of the art biophysical approaches to issues in glycobiology that have cutting-edge applications. Despite the importance of glycosylation, the complexity, heterogeneity, and flexibility of the glycans have inhibited their study. Each chapter in this book explains very recent significant advances in biophysical approaches through the use of techniques such as NMR spectroscopy, mass spectrometry, single-molecule imaging, X-ray crystallography, high-speed atomic force microscopy, and computational simulation and their integrative application. Concrete examples are provided of the value of these techniques in addressing key problems in the field. In addition, significant functional glycobiological issues are considered. For example, glycolipids can form dynamic clusters on cell membranes and provide platforms for molecules involved in cell recognition and subsequent signal transduction. The detailed delineation of these molecular systems is discussed, revealing their structural complexity and ability to assemble transiently. This timely book will be of value for graduate students and postdocs interested in frontier topics in glycoscience and also for senior bio-researchers in academic and industrial fields.

Trace Determination of Glycoproteins by Mass Spectrometry Coupled to High Performance Liquid Chromatography and Capillary Electrophoresis

Download Trace Determination of Glycoproteins by Mass Spectrometry Coupled to High Performance Liquid Chromatography and Capillary Electrophoresis PDF Online Free

Author : Chuanliang Liu
Publisher :
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Trace Determination of Glycoproteins by Mass Spectrometry Coupled to High Performance Liquid Chromatography and Capillary Electrophoresis by : Chuanliang Liu

Download or read book Trace Determination of Glycoproteins by Mass Spectrometry Coupled to High Performance Liquid Chromatography and Capillary Electrophoresis written by Chuanliang Liu and published by . This book was released on 1995 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: