Light Sheet Fluorescence Microscopy

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Light Sheet Fluorescence Microscopy

Author : Emmanuel G. Reynaud
Publisher : John Wiley & Sons
ISBN 13 : 3527341358
Total Pages : 421 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Light Sheet Fluorescence Microscopy by : Emmanuel G. Reynaud

Download or read book Light Sheet Fluorescence Microscopy written by Emmanuel G. Reynaud and published by John Wiley & Sons. This book was released on 2024-04-08 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: An indispensable guide to a novel, revolutionary fluorescence microscopy technique! Light sheet-based fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a “do-it-yourself” light sheet microscope, making the technique affordable for every laboratory. This important book: Serves as an easy-to-understand introduction to light sheet-based fluroescence Includes numerous tips and tricks for advanced practitioners Provides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi Aimed at cell biologists, biophysicists, developmental biologists, and neurobiologists, Light Sheet-based Fluorescence Microscopy offers a comprehensive overview of the and the most recent applications of this microscopy technique.

Fluorescence Microscopy

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
ISBN 13 : 3527687726
Total Pages : 508 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck

Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-03-27 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

Light Sheet Based Fluorescence Microscopy

Author : Ernst H. K. Stelzer
Publisher : Wiley-Blackwell
ISBN 13 : 9783527321131
Total Pages : 250 pages
Book Rating : 4.3/5 (211 download)

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Book Synopsis Light Sheet Based Fluorescence Microscopy by : Ernst H. K. Stelzer

Download or read book Light Sheet Based Fluorescence Microscopy written by Ernst H. K. Stelzer and published by Wiley-Blackwell. This book was released on 2013-08-05 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book introducing a revolutionary new imaging technology, light sheet fluorescence microscopy. Written in a comprehensive fashion by the same people who developed this technique, this treatise is a must have for everyone who plans to work with the new technology.

Fluorescence Microscopy

Author : Anda Cornea
Publisher : Elsevier
ISBN 13 : 0124167136
Total Pages : 261 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Fluorescence Microscopy by : Anda Cornea

Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Quantitative Imaging in Cell Biology

Author :
Publisher : Academic Press
ISBN 13 : 0124202012
Total Pages : 609 pages
Book Rating : 4.1/5 (242 download)

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Book Synopsis Quantitative Imaging in Cell Biology by :

Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

The Light-Sheet Microscopy

Author : Rolf Theodor Borlinghaus
Publisher : Springer Nature
ISBN 13 : 3658327685
Total Pages : 38 pages
Book Rating : 4.6/5 (583 download)

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Book Synopsis The Light-Sheet Microscopy by : Rolf Theodor Borlinghaus

Download or read book The Light-Sheet Microscopy written by Rolf Theodor Borlinghaus and published by Springer Nature. This book was released on 2021-06-01 with total page 38 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential explains what distinguishes light sheet microscopy from ordinary light microscopy. The author briefly examines the history of such principles, focusing on the technical concepts. Finally, current manifestations are presented without descending into the depths of the art of engineering. The unusual feature of light-sheet microscopy is not only that observation and illumination take place at a right angle, but also that this type of microscopy gains in particular from the fact that the type of illumination only passes through a very small part of the specimen. The appropriate selection of optical elements ensures that the observed image no longer contains any blurred parts. This Springer essential is a translation of the original German 1st edition essentials, Die Lichtblattmikroskopie by Rolf Theodor Borlinghaus, published by Springer Fachmedien Wiesbaden GmbH, part of Springer Nature in 2017. The translation was done with the help of artificial intelligence (machine translation by the service DeepL.com). A subsequent human revision was done primarily in terms of content, so that the book will read stylistically differently from a conventional translation. Springer Nature works continuously to further the development of tools for the production of books and on the related technologies to support the authors.

High Resolution Imaging in Microscopy and Ophthalmology

Author : Josef F. Bille
Publisher : Springer
ISBN 13 : 3030166384
Total Pages : 407 pages
Book Rating : 4.0/5 (31 download)

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Book Synopsis High Resolution Imaging in Microscopy and Ophthalmology by : Josef F. Bille

Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Colloids and the Ultramicroscope

Author : Richard Adolf Zsigmondy
Publisher :
ISBN 13 :
Total Pages : 290 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Colloids and the Ultramicroscope by : Richard Adolf Zsigmondy

Download or read book Colloids and the Ultramicroscope written by Richard Adolf Zsigmondy and published by . This book was released on 1909 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Understanding Light Microscopy

Author : Jeremy Sanderson
Publisher : John Wiley & Sons
ISBN 13 : 0470973757
Total Pages : 848 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Understanding Light Microscopy by : Jeremy Sanderson

Download or read book Understanding Light Microscopy written by Jeremy Sanderson and published by John Wiley & Sons. This book was released on 2019-05-06 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Microscopy Techniques

Author : Jens Rietdorf
Publisher : Springer Science & Business Media
ISBN 13 : 9783540236986
Total Pages : 344 pages
Book Rating : 4.2/5 (369 download)

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Book Synopsis Microscopy Techniques by : Jens Rietdorf

Download or read book Microscopy Techniques written by Jens Rietdorf and published by Springer Science & Business Media. This book was released on 2005-06-23 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts

Introduction to Optical Microscopy

Author : Jerome Mertz
Publisher : Cambridge University Press
ISBN 13 : 1108428304
Total Pages : 475 pages
Book Rating : 4.1/5 (84 download)

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Book Synopsis Introduction to Optical Microscopy by : Jerome Mertz

Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Fast Image Restoration in Light-sheet Fluorescence Microscopy with Extended Depth of Field Using GPUs

Author : David Castillo Andreo
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (112 download)

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Book Synopsis Fast Image Restoration in Light-sheet Fluorescence Microscopy with Extended Depth of Field Using GPUs by : David Castillo Andreo

Download or read book Fast Image Restoration in Light-sheet Fluorescence Microscopy with Extended Depth of Field Using GPUs written by David Castillo Andreo and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Light sheet fluorescence microscopy (LSFM) is used in many biological research experiments that require fast three-dimensional (3D) imaging up to a few volumes per second. Wavefront coding (WFC) microscopy provides LSFM with 3D real-time imaging capabilities introducing a controlled aberration that extends the depth of field (DOF) of the microscope objective. Resulting images, however, are blurred and need to be processed to recover the original sharpness in a CPU-intensive deconvolution which makes real-time visualization unattainable. We present computational tools based on GPU parallel computing to achieve real-time deconvolution and visualization of the images obtained with WFC light-sheet microscopy.

Fluorescence Microscopy

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
ISBN 13 : 3527338373
Total Pages : 504 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck

Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-06-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

Fluorescence Microscopy in Life Sciences

Author : Juan Carlos Stockert
Publisher : Bentham Science Publishers
ISBN 13 : 1681085186
Total Pages : 751 pages
Book Rating : 4.6/5 (81 download)

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Book Synopsis Fluorescence Microscopy in Life Sciences by : Juan Carlos Stockert

Download or read book Fluorescence Microscopy in Life Sciences written by Juan Carlos Stockert and published by Bentham Science Publishers. This book was released on 2017-12-15 with total page 751 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

The Proceedings of the International Conference on Sensing and Imaging

Author : Ming Jiang
Publisher : Springer
ISBN 13 : 3319916599
Total Pages : 421 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis The Proceedings of the International Conference on Sensing and Imaging by : Ming Jiang

Download or read book The Proceedings of the International Conference on Sensing and Imaging written by Ming Jiang and published by Springer. This book was released on 2018-09-18 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book collects a number of papers presented at the International Conference on Sensing and Imaging, which was held at Chengdu University of Information Technology on June 5-7, 2017. Sensing and imaging is an interdisciplinary field covering a variety of sciences and techniques such as optics, electricity, magnetism, heat, sound, mathematics, and computing technology. The field has diverse applications of interest such as sensing techniques, imaging, and image processing techniques. This book will appeal to professionals and researchers within the field.

Cell Imaging Techniques

Author : Douglas J. Taatjes
Publisher : Springer Science & Business Media
ISBN 13 : 1592599931
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Cell Imaging Techniques by : Douglas J. Taatjes

Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Superresolution Optical Microscopy

Author : Barry R. Masters
Publisher : Springer
ISBN 13 : 9783030216931
Total Pages : 0 pages
Book Rating : 4.2/5 (169 download)

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Book Synopsis Superresolution Optical Microscopy by : Barry R. Masters

Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer. This book was released on 2021-08-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.