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Investigation Of Some Technical Aspects Of Horseradish Peroxidase And Alkaline Phosphatase Conjugates For Use In Elisa Tests Microform
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Book Synopsis Investigation of Some Technical Aspects of Horseradish Peroxidase and Alkaline Phosphatase Conjugates for Use in ELISA Tests [microform] by : Robin Kim King
Download or read book Investigation of Some Technical Aspects of Horseradish Peroxidase and Alkaline Phosphatase Conjugates for Use in ELISA Tests [microform] written by Robin Kim King and published by National Library of Canada. This book was released on 1982 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The following study was undertaken to produce enzyme-conjugates to be used for antigen and antibody detection of Coxsackieviruses B1-B6 and for the detection of Western Equine Encephalomyelitis virus in animal and arthropod tissues. Anti-Coxsackieviruses B1-B6 immune sera were obtained from guinea pigs immunized with viral antigens prepared in HeLa cells and anti-Western Equine Encephalomyelitis virus was prepared in mice using antigen prepared in suckling mouse brains. Antiguinea pig and anti-mouse enzyme-labelled conjugates were prepared using horseradish peroxidase and alkaline phosphatase by a periodate and a one-step glutaraldehyde conjugation procedure respectively. The horseradish peroxidase conjugate prepared failed to react in the ELISA test and yields were very low with little enzymatic activity. Conjugates prepared using alkaline phosphatase had enzymatic activities ranging from 195-510 IU/mL and were titred using polysterene microtitre plates coated with 10 yg/mL immunoglobulin. The conjugate titres correspondingly ranged from 1:100 to 1:500 depending on the enzymatic activity. Retention of the original enzymatic activity ranged from 16-65%. Passage of the conjugates through Sephacryl S-300 superfine gel filtration helped remove unconjugated enzyme and immunoglobulin from the conjugate. The alkaline phosphatase conjugates were used to titrate Coxsackieviruses B1-B6 using checkerboard titration systems. A positive test value was taken as two times the absorbance of the normal HeLa cell control provided the latter value was equal to or less than 0.150 units. The optimal antigen concentration was at a 10" 3 dilution of the infected HeLa cell cultures except for Coxsackievirus B3 which reacted optimally at a 10" 2 dilution. No reaction was evident in any of the control wells and their absorbances were zero. The antisera titres ranged from 1:80 to 1:1280 and these endpoints were clearly defined. Secondary experiments employing Coxsackie B-virus antigens in AGMK cell culture resulted in gross non-specific findings which were attributed to non-specific protein binding of the polysterene plates. Preliminary testing of the plates using 1% bovine albumin in the diluents employed during the assay failed to reduce the non-specificity of the plastics arid work is ongoing to solve the problem. The ELISA has shown applicability to Coxsackie B-virus detection and, likewise, should be an efficient method of detecting Western Equine Encephalomyelitis virus once the parameters of testing have been completely worked out.
Download or read book Canadiana written by and published by . This book was released on 1985 with total page 1158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis SINGLE MOLECULE STUDIES OF ENZYMES HORSERADISH PEROXIDASE AND ALKALINE PHOSPHATASE USING TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY AND CONFOCAL MICROSCOPY by : Leonora Kaldaras
Download or read book SINGLE MOLECULE STUDIES OF ENZYMES HORSERADISH PEROXIDASE AND ALKALINE PHOSPHATASE USING TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY AND CONFOCAL MICROSCOPY written by Leonora Kaldaras and published by . This book was released on 2013 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conformational dynamics plays an important role in determining protein reactivity including affinity to substrate and product, catalytic rate constant and product release process. Whereas the influence of conformational dynamics on kinetic parameters of enzymatic reaction have been widely studied by ensemble averaging techniques, in these experiments conformational variations tend to average out and therefore ensemble averaging methods lack the level of resolution necessary to characterize time dependent behavior of individual enzyme molecules, which is essential to understanding enzymatic catalysis. This thesis consists of two parts. The first part focuses on the new development of integrated spectroscopy system, which combined the advantages of wide plane view field of objective-type TIRFM imaging with high time-resolution confocal single-molecule spectroscopy to address the challenge of probing both temporally and spatially stochastic events of the single-molecule reactions, such as fluorogenic enzymatic reactions with enzyme tethered to a solution/glass interface. Tthe calibration of this integrated apparatus is demonstrated with fluorescence microspheres and the feasibility by spectroscopy measurement of single molecule Rhodamine 6G on glass surface. Finally, the application of the apparatus is demonstrated by studying spatially and temporally randomly distributed single-molecule enzymatic reaction of fluorogenic substrate, horseradish peroxidase-catalyzed oxidation of amplex red. The second part focuses on exploratory studies of enzyme Alkaline Phosphatase from E. coli and its potential to be studied on single molecule level using fluorogenic substrate 3-o-methylfluorescein phosphate (MFP) and two polarization total internal reflection fluorescence microscopy apparatus. Ensemble averaged kinetics data and several single molecule trajectories are presented here.
Book Synopsis Minimal Residual Disease in Melanoma by : U. Reinhold
Download or read book Minimal Residual Disease in Melanoma written by U. Reinhold and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge about diagnostic procedures in melanoma has in creased rapidly within the past few years. Single tumor cells have been identified in normal tissue such as sentinel lymph nodes, as well as in bone marrow, peripheral blood, and other bodily fluids and cells, by molecular technologies. The introduction of polymerase chain reaction-based methods can be regarded as a prototype of this dramatic development towards molecular approaches in new diagnostic procedures. This fact opens up the possibility of clinical use in patients and of influencing treatment strategies. Considerable discrepancies have been described, how ever, in the success rates of these new techniques for the detec tion of minimal residual disease in cancer patients. Despite fav orable results reported by different groups of investigators, it will take several years to define the clinical and pathophysiological relevance of new diagnostic procedures. The 1st International Symposium "Minimal Residual Disease in Melanoma: Biology, Detection and Clinical Relevance of Microme tastases", held in September 1999 in Homburg/Saar, Germany, fo cused on recent developments in this particular area of cancer re search. The purpose of the meeting was to stimulate discussion and exchange of new data and ideas by renowned international scientists. The aim of this volume is to summarize major topics of basic research and clinical investigations presented by invited experts in this fascinating but still. controversial field of melanoma re search.
Book Synopsis Photoproteins in Bioanalysis by : Sylvia Daunert
Download or read book Photoproteins in Bioanalysis written by Sylvia Daunert and published by John Wiley & Sons. This book was released on 2006-12-13 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Book Synopsis Technology in Forensic Science by : Deepak Rawtani
Download or read book Technology in Forensic Science written by Deepak Rawtani and published by John Wiley & Sons. This book was released on 2020-11-02 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book "Technology in Forensic Science" provides an integrated approach by reviewing the usage of modern forensic tools as well as the methods for interpretation of the results. Starting with best practices on sample taking, the book then reviews analytical methods such as high-resolution microscopy and chromatography, biometric approaches, and advanced sensor technology as well as emerging technologies such as nanotechnology and taggant technology. It concludes with an outlook to emerging methods such as AI-based approaches to forensic investigations.
Book Synopsis Japanese Encephalitis and West Nile Viruses by : John Mackenzie
Download or read book Japanese Encephalitis and West Nile Viruses written by John Mackenzie and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Japanese encephalitis and West Nile viruses are members of the Japanese encephalitis serological group of the genus Flavivirus and therefore closely related genetically and antigenically. They share a number of properties, including the use of birds as their major wildlife maintenance host and Culicine mosquitoes for transmission, and they are both associated with severe human disease, as well as fatal infections in horses. The emergence of these two viruses, and their well-established propensity to colonise new areas, make it timely to re-examine their ecology, biology, molecular structure, replication and epidemiology, and these therefore provide the focus of this volume.
Book Synopsis Antibody Engineering Volume 2 by : Roland E. Kontermann
Download or read book Antibody Engineering Volume 2 written by Roland E. Kontermann and published by Springer Science & Business Media. This book was released on 2010-03-10 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.
Book Synopsis Laboratory Guide to the Methods in Biochemical Genetics by : Nenad Blau
Download or read book Laboratory Guide to the Methods in Biochemical Genetics written by Nenad Blau and published by Springer Science & Business Media. This book was released on 2008-05-31 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory.
Book Synopsis Basic Techniques in Molecular Biology by : Stefan Surzycki
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Book Synopsis Enzymes in Industry by : Wolfgang Aehle
Download or read book Enzymes in Industry written by Wolfgang Aehle and published by John Wiley & Sons. This book was released on 2008-01-08 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading experts from all over the world present an overview of the use of enzymes in industry for: - the production of bulk products, such as glucose, or fructose - food processing and food analysis - laundry and automatic dishwashing detergents - the textile, pulp and paper and animal feed industries - clinical diagnosis and therapy - genetic engineering. The book also covers identification methods of new enzymes and the optimization of known ones, as well as the regulatory aspects for their use in industrial applications. Up to date and wide in scope, this is a chance for non-specialists to acquaint themselves with this rapidly growing field. '...The quality...is so great that there is no hesitation in recommending it as ideal reading for any student requiring an introduction to enzymes. ...Enzymes in Industry - should command a place in any library, industrial or academic, where it will be frequently used.' The Genetic Engineer and Biotechnologist 'Enzymes in Industry' is an excellent introduction into the field of applied enzymology for the reader who is not familiar with the subject. ... offers a broad overview of the use of enzymes in industrial applications. It is up-to-date and remarkable easy to read, despite the fact that almost 50 different authors contributed. The scientist involved in enzyme work should have this book in his or her library. But it will also be of great value to the marketing expert interested in the present use of enzymes and their future in food and nonfood applications.' Angewandte Chemie 'This book should be available to all of those working with, or aspiring to work with, enzymes. In particular academics should use this volume as a source book to ensure that their 'new' projects will not 'reinvent the wheel'.' Journal of Chemical Technology and Biotechnology
Book Synopsis Dorland's Dictionary of Medical Acronyms and Abbreviations E-Book by : Dorland
Download or read book Dorland's Dictionary of Medical Acronyms and Abbreviations E-Book written by Dorland and published by Elsevier Health Sciences. This book was released on 2015-07-24 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medical acronyms and abbreviations offer convenience, but those countless shortcuts can often be confusing. Now a part of the popular Dorland’s suite of products, this reference features thousands of terms from across various medical specialties. Its alphabetical arrangement makes for quick reference, and expanded coverage of symbols ensures they are easier to find. Effective communication plays an important role in all medical settings, so turn to this trusted volume for nearly any medical abbreviation you might encounter. Symbols section makes it easier to locate unusual or seldom-used symbols. Convenient alphabetical format allows you to find the entry you need more intuitively. More than 90,000 entries and definitions. Many new and updated entries including terminology in expanding specialties, such as Nursing; Physical, Occupational, and Speech Therapies; Transcription and Coding; Computer and Technical Fields. New section on abbreviations to avoid, including Joint Commission abbreviations that are not to be used. Incorporates updates suggested by the Institute for Safe Medication Practices (ISMP).
Author :Monika Schäfer-Korting Publisher :Springer Science & Business Media ISBN 13 :3642004776 Total Pages :506 pages Book Rating :4.6/5 (42 download)
Book Synopsis Drug Delivery by : Monika Schäfer-Korting
Download or read book Drug Delivery written by Monika Schäfer-Korting and published by Springer Science & Business Media. This book was released on 2010-03-10 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the view of most experts pharmacology is on drugs, targets, and actions. In the context the drug as a rule is seen as an active pharmaceutical ingredient and not as a complex mixture of chemical entities of a well defined structure. Today, we are becoming more and more aware of the fact that delivery of the active compound to the target site is a key. The present volume gives a topical overview on various modern approaches to drug targeting covering today’s options for specific carrier systems allowing successful drug treatment at various sites of the body difficult to address and allowing to increase the benefit-risk-ratio to the optimum possible.
Book Synopsis Animal Cell Culture Techniques by : Martin Clynes
Download or read book Animal Cell Culture Techniques written by Martin Clynes and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Book Synopsis Tissue Culture by : Paul F. Jr. Kruse
Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 897 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Book Synopsis Pharmacokinetics of Drugs by : Peter G. Welling
Download or read book Pharmacokinetics of Drugs written by Peter G. Welling and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: A compilation of researchers' experience in the areas of bioanalysis, pharmacokinetics, and drug metabolism, to present an up-to-date and comprehensive treatise on the application of these and related technologies in drug discovery, development, and clinical use. Contents cover descriptions of analytical methods, in vitro metabolism technology and membrane transport, reappraisal of classical pharmacokinetic problems, and the time course of drug action. The book concludes with a description of PET and imaging methods in pharmacokinetics and an appendix containing a critical appraisal of computer methods and pharmacokinetic software available for PCs.
Book Synopsis Antibody Engineering Volume 1 by : Roland E. Kontermann
Download or read book Antibody Engineering Volume 1 written by Roland E. Kontermann and published by Springer Science & Business Media. This book was released on 2010-03-10 with total page 770 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.
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