Integrin Protocols

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Integrin Protocols

Author : Anthony R. Howlett
Publisher : Springer Science & Business Media
ISBN 13 : 159259249X
Total Pages : 296 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Integrin Protocols by : Anthony R. Howlett

Download or read book Integrin Protocols written by Anthony R. Howlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Integrin Protocols, Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the RNA and protein levels-and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. The emphasis is always on the practical steps necessary for experimental success and robust results. Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis, as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.

The Integrin Interactome

Author : Miguel Vicente-Manzanares
Publisher : Humana
ISBN 13 : 9781071609613
Total Pages : 315 pages
Book Rating : 4.6/5 (96 download)

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Book Synopsis The Integrin Interactome by : Miguel Vicente-Manzanares

Download or read book The Integrin Interactome written by Miguel Vicente-Manzanares and published by Humana. This book was released on 2020-11-20 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.

The Integrin Interactome

Author : Miguel Vicente-Manzanares
Publisher : Humana
ISBN 13 : 9781071609644
Total Pages : 315 pages
Book Rating : 4.6/5 (96 download)

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Book Synopsis The Integrin Interactome by : Miguel Vicente-Manzanares

Download or read book The Integrin Interactome written by Miguel Vicente-Manzanares and published by Humana. This book was released on 2021-12-04 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.

Integrin and Cell Adhesion Molecules

Author : Motomu Shimaoka
Publisher : Humana Press
ISBN 13 : 9781493958085
Total Pages : 529 pages
Book Rating : 4.9/5 (58 download)

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Book Synopsis Integrin and Cell Adhesion Molecules by : Motomu Shimaoka

Download or read book Integrin and Cell Adhesion Molecules written by Motomu Shimaoka and published by Humana Press. This book was released on 2016-08-23 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.

Integrins

Author :
Publisher : Elsevier
ISBN 13 : 0080917291
Total Pages : 297 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Integrins by :

Download or read book Integrins written by and published by Elsevier. This book was released on 2012-12-02 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrins: Molecular and Biological Responses to the Extracellular Matrix will help basic, applied, and clinical researchers keep up with the explosion of literature on the integrin family of proteins. This volume extends material previously covered in Receptors for Extracellular Matrix. It addresses some of the most exciting areas of integrin biology, including the varied roles of integrins in cell division, differentiation, movement, wound healing, inflammation, thrombosis, osteoporosis, and cancer. Describes key aspects of integrin structure, function, and biology Covers collagen receptors, epithelial cell integrins, leukocyte integrins, platelet integrins, integrin signaling, and integrin antagonists Investigates the expression and role of integrins during development and in the cytoskeleton Includes the actions and influences of integrins in inflammation, thrombosis, and osteoporosis

Integrin Targeting Systems for Tumor Diagnosis and Therapy

Author : Eleonora Patsenker
Publisher : Humana
ISBN 13 : 9781493992478
Total Pages : 226 pages
Book Rating : 4.9/5 (924 download)

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Book Synopsis Integrin Targeting Systems for Tumor Diagnosis and Therapy by : Eleonora Patsenker

Download or read book Integrin Targeting Systems for Tumor Diagnosis and Therapy written by Eleonora Patsenker and published by Humana. This book was released on 2019-05-17 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume collects a variety of techniques and methodologies developed to facilitate research on integrin biology and to identify ideal targets and approaches for the treatment of multiple organ diseases, with a focus on cancer in particular. The chapters consecutively describe the tools for structural analysis, identification and detection of integrins as biomarkers, and include thorough laboratory and clinically-related methods on different strategies for generation, synthesis and evaluation of probes, carriers, peptides or small particles for integrin targeting, imaging, and drug delivery. As part of the Methods in Pharmacology and Toxicology series, this book contains the practical details that are invaluable in the laboratory. Authoritative and advantageous, Integrin Targeting Systems for Tumor Diagnosis and Therapy serves readers from a wide spectrum, including researchers and students seeking an overview of existing developments, as well as leading professionals aiming to become more familiar with integrin-related innovative technologies in cancer research.

Adhesion Protein Protocols

Author : Amanda S. Coutts
Publisher : Springer Science & Business Media
ISBN 13 : 1597453536
Total Pages : 237 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Adhesion Protein Protocols by : Amanda S. Coutts

Download or read book Adhesion Protein Protocols written by Amanda S. Coutts and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.

Integrin-Ligand Interaction

Author : Johannes A. Elbe
Publisher : Springer Science & Business Media
ISBN 13 : 1475740646
Total Pages : 277 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Integrin-Ligand Interaction by : Johannes A. Elbe

Download or read book Integrin-Ligand Interaction written by Johannes A. Elbe and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt:

T Cell Protocols

Author : Kelly P. Kearse
Publisher : Springer Science & Business Media
ISBN 13 : 1592596827
Total Pages : 357 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis T Cell Protocols by : Kelly P. Kearse

Download or read book T Cell Protocols written by Kelly P. Kearse and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology. Invariably, in putting together a book like this it is difficult to decide which methods to include and which to leave out. To this end methods were selected from a variety of disciplines, including cellular immunology, b- chemistry, and molecular biology, to try to provide something of interest for everyone who works on T-cell development and activation. I would like to mention that my primary reason for agreeing to put this book together is that, when I was a graduate student, I purchased a copy of Selected Methods in Cellular Immunology by Mishell and Shigii which proved a tremendous help in learning the basics of one-and two- dimensional gel te- niques (and other methods). The cover has long since fallen off, but it still remains one of my most valued reference books for the laboratory. It is my hope that T Cell Protocols: Development and Activation will prove similarly useful to current and future scientists wishing to learn new methods for expl- ing the development and activation of T cells.

Integrin Protocols

Author : Anthony R. Howlett
Publisher : Humana Press
ISBN 13 : 9780896035690
Total Pages : 296 pages
Book Rating : 4.0/5 (356 download)

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Book Synopsis Integrin Protocols by : Anthony R. Howlett

Download or read book Integrin Protocols written by Anthony R. Howlett and published by Humana Press. This book was released on 1999-07-15 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the RNA and protein levels-and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.

I Domain Integrins

Author : Donald Gullberg
Publisher : Springer
ISBN 13 : 9401791538
Total Pages : 188 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis I Domain Integrins by : Donald Gullberg

Download or read book I Domain Integrins written by Donald Gullberg and published by Springer. This book was released on 2014-07-14 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: The integrin family is composed of 24 members and approximately ten years ago (2003) we published a book devoted to the nine I domain integrin subunits. In this second edition, I am pleased that most of the original authors have been able to contribute to the updated version. I domain containing integrins include collagen receptors and leukocyte receptors. In 2003 the knockout mouse phenotypes for all of the I domain integrins had not yet been published; they are now, and are summarized and discussed in this edition. Interestingly, a recent 10 integrin mutation in dogs has indicated that collagen-binding integrins in the musculoskeletal system might have much more severe phenotypes in larger animals/humans compared to the mild integrin phenotypes observed in collagen-binding integrin deficient mice. This finding is further discussed in the book. In the cancer field, the microenvironment is taking center stage, and here collagen receptors on fibroblasts are predicted to play important roles in paracrine signaling, in regulating tissue stiffness and matrix remodeling. New technologies, new mouse models in combination with analyses of I integrins in larger animals/humans are thus predicted to increase our knowledge about this group of receptors. With this in mind we look forward to another 10 years of research with I domain integrins.

Integrins

Author :
Publisher : Elsevier
ISBN 13 : 9780080551388
Total Pages : 616 pages
Book Rating : 4.5/5 (513 download)

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Book Synopsis Integrins by :

Download or read book Integrins written by and published by Elsevier. This book was released on 2007-08-27 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: An integrin, or integrin receptor, is an integral membrane protein in the plasma membrane of cells. It plays a role in the attachment of a cell to the extracellular matrix (ECM) and to other cells, and in signal transduction from the ECM to the cell. There are many types of integrin, and many cells have multiple types on their surface. Integrins are of vital importance to all metazoans, from humans to sponges. This volume in Methods in Enzymology presents methods for studying integrins.

Developmental Biology Protocols

Author : Rocky S. Tuan
Publisher : Springer Science & Business Media
ISBN 13 : 1592590659
Total Pages : 537 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Developmental Biology Protocols by : Rocky S. Tuan

Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Calpain Methods and Protocols

Author : John S. Elce
Publisher : Springer Science & Business Media
ISBN 13 : 1592590500
Total Pages : 340 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Calpain Methods and Protocols by : John S. Elce

Download or read book Calpain Methods and Protocols written by John S. Elce and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of Calpain Methods and Protocols is quite straightf- ward: it is to present the actual experimental methods used in many different laboratories for the study of calpain. It will provide the vital experimental detail, and the discussion of possible pitfalls, for which the standard journals no longer provide space. This will make it as easy as possible for investi- tors interested in calpain to adopt established methods without repeating old mistakes, and to adapt and apply these methods in novel approaches to the many outstanding calpain questions. These questions range from purely biochemical problems of protein structure and enzyme regulation at the molecular level, through large areas of cell biology, to applied and clinical aspects of calpain function in human d- ease. Within this panoply of topics, a wide range of investigators will find many fascinating and as yet unanswered questions about calpain. Calpain Methods and Protocols will provide instant access to many essential te- niques, while saving them the time and effort involved in developing a new method. In addition to questions relating to the normal physiological roles of the calpains, there is considerable evidence that inappropriate calpain activity may have pathological effects in many tissues, for example, following ischemia. This provides a major stimulus for the development of specific calpain inhi- tors for therapeutic purposes, and for the development of methods to evaluate such inhibitors.

Flavoprotein Protocols

Author : Steven K. Chapman
Publisher : Springer Science & Business Media
ISBN 13 : 159259266X
Total Pages : 257 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Flavoprotein Protocols by : Steven K. Chapman

Download or read book Flavoprotein Protocols written by Steven K. Chapman and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.

Metastasis Research Protocols

Author : Susan A. Brooks
Publisher : Springer Science & Business Media
ISBN 13 : 1592591361
Total Pages : 323 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Metastasis Research Protocols by : Susan A. Brooks

Download or read book Metastasis Research Protocols written by Susan A. Brooks and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: The process of metastasis formation is hugely complex, as described in the introductory chapter of this book, and this complexity has led us to compile two volumes of methods, from a vastly divergent background that attempts to encompass the whole spectrum of cancer biology. This first volume, Metastasis Research Protocols: Analysis of Cells and Tissues, concentrates on analysis and mapping of molecules produced by cells and tissues and analysis of the molecular biology underlying their expression, whereas the second volume, Metastasis Research Protocols: Cell Behavior In Vitro and In Vivo,focuses sharply on the determination of cell behavior in vitro and in vivo. We have deliberately included chapters describing well-established and familiar te- niques (for example, SDS-PAGE and Western blotting [Chapter 11], and immunocytochemistry [Chapter 2]) in addition to the newer and more speci- ized approaches and specific examples of their application, because—although the methodology is readily available in the published literature and established in many laboratories—we wished these volumes to “stand alone” and to make accessible here the standard techniques that underpin much metastasis research for both the newcomer to the field and the seasoned researcher. Undoubtedly, owing to the complexity of the metastatic cascade and the wealth of research techniques involved in scientific approaches to its unraveling, and despite our best efforts to make these volumes as comprehensive as seems feasible, this is a tall order, and there will inevitably be omissions. For these we apologize.

Integrins in Health and Disease

Author : Donald Gullberg
Publisher : Springer
ISBN 13 : 9783031237836
Total Pages : 0 pages
Book Rating : 4.2/5 (378 download)

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Book Synopsis Integrins in Health and Disease by : Donald Gullberg

Download or read book Integrins in Health and Disease written by Donald Gullberg and published by Springer. This book was released on 2024-03-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrins are heterodimeric cell surface receptors which anchor cells to different extracellular matrix proteins or act as cell-cell receptors. They play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, wound healing, and regulation of cell growth, but also in numerous pathological conditions such as autoimmunity, infectious disease, and carcinogenesis. This book aims to provide readers a summary of the most important integrins and their respective biological functions. Readers will learn about knockout- and animal models to study the functionality of key collagen-, laminin-, and nephronectin-binding integrins. Additionally, the role of integrins in pathological tissue remodeling in joints and in developing and diseased cardiac tissue are discussed. Reviews of the current knowledge of the role of integrins in tissue and tumor fibrosis, angiogenesis and tumor progression are an important part of this work. Finally, the book discusses integrins in the context of the immune system, how to target integrin-ligand interactions with antibodies, and the role of integrins as receptors for bacterial and viral cell invasion. Both experienced researchers and clinicians, as well as PhD students who wish to study the extracellular matrix and cell adhesion molecules will find “Integrins in Health and Disease - Key Effectors of Cell-Matrix and Cell-Cell Interactions” authoritative, easily accessible, and vastly informative. The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology and the International Society for Matrix Biology.