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In Vitro Characterization Of The Transcript Cleavage Reaction Of Ecoli Rna Polymerase
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Book Synopsis In Vitro Characterization of the Transcript Cleavage Reaction of E.coli RNA Polymerase by : Jeremy William Burt
Download or read book In Vitro Characterization of the Transcript Cleavage Reaction of E.coli RNA Polymerase written by Jeremy William Burt and published by . This book was released on 2003 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biochemical and Genetic Characterization of the Transcription Elongation Factor TFIIS from the Yeast Saccharomyces Cerevisiae by : Karen Renee Christie
Download or read book Biochemical and Genetic Characterization of the Transcription Elongation Factor TFIIS from the Yeast Saccharomyces Cerevisiae written by Karen Renee Christie and published by . This book was released on 1995 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of the process of transcriptional elongation is an important control mechanism in the expression of some genes. To fully understand this form of regulation will require better understanding of the functions of transcription elongation factors. The goal of this work was to characterize the transcription elongation factor TFIIS from Saccharomyces cerevisiae, originally called P37. I demonstrated that, like the mammalian TFIIS proteins, the yeast protein stimulates RNA polymerase II to cleave the nascent RNA transcript and to read-through an intrinsic block to elongation. Investigation of the protein-protein contacts between TFIIS and RNA polymerase II indicated that the carboxyl-terminal domain of the largest subunit, subunit four, and subunit seven of the polymerase are not required for TFIIS to promote cleavage and read-through by the polymerase. In addition the carboxyl-terminal half of the yeast TFIIS protein is sufficient for both of these in vitro activities. This result is consistent with the previous results demonstrating the carboxyl-terminus of mouse TFIIS was sufficient to activate RNA polymerase in vitro.
Book Synopsis Guide to Yeast Genetics and Molecular Biology by : Christine Guthrie
Download or read book Guide to Yeast Genetics and Molecular Biology written by Christine Guthrie and published by . This book was released on 1991-01-28 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein
Book Synopsis Genetic and Genomic Analyses of the Saccharomyces Cerevisiae Transcript Elongation Factor, TFIIS by : Caren Jody Stark
Download or read book Genetic and Genomic Analyses of the Saccharomyces Cerevisiae Transcript Elongation Factor, TFIIS written by Caren Jody Stark and published by . This book was released on 2003 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2004 with total page 882 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lasso Peptides written by Yanyan Li and published by Springer. This book was released on 2014-10-21 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lasso peptides form a growing family of fascinating ribosomally-synthesized and post-translationally modified peptides produced by bacteria. They contain 15 to 24 residues and share a unique interlocked topology that involves an N-terminal 7 to 9-residue macrolactam ring where the C-terminal tail is threaded and irreversibly trapped. The ring results from the condensation of the N-terminal amino group with a side-chain carboxylate of a glutamate at position 8 or 9, or an aspartate at position 7, 8 or 9. The trapping of the tail involves bulky amino acids located in the tail below and above the ring and/or disulfide bridges connecting the ring and the tail. Lasso peptides are subdivided into three subtypes depending on the absence (class II) or presence of one (class III) or two (class I) disulfide bridges. The lasso topology results in highly compact structures that give to lasso peptides an extraordinary stability towards both protease degradation and denaturing conditions. Lasso peptides are generally receptor antagonists, enzyme inhibitors and/or antibacterial or antiviral (anti-HIV) agents. The lasso scaffold and the associated biological activities shown by lasso peptides on different key targets make them promising molecules with high therapeutic potential. Their application in drug design has been exemplified by the development of an integrin antagonist based on a lasso peptide scaffold. The biosynthesis machinery of lasso peptides is therefore of high biotechnological interest, especially since such highly compact and stable structures have to date revealed inaccessible by peptide synthesis. Lasso peptides are produced from a linear precursor LasA, which undergoes a maturation process involving several steps, in particular cleavage of the leader peptide and cyclization. The post-translational modifications are ensured by a dedicated enzymatic machinery, which is composed of an ATP-dependent cysteine protease (LasB) and a lactam synthetase (LasC) that form an enzymatic complex called lasso synthetase. Microcin J25, produced by Escherichia coli AY25, is the archetype of lasso peptides and the most extensively studied. To date only around forty lasso peptides have been isolated, but genome mining approaches have revealed that they are widely distributed among Proteobacteria and Actinobacteria, particularly in Streptomyces, making available a rich resource of novel lasso peptides and enzyme machineries towards lasso topologies.
Book Synopsis Regulation of Gene Expression in Escherichia coli by : E. C. C. Lin
Download or read book Regulation of Gene Expression in Escherichia coli written by E. C. C. Lin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.
Author :Andrew Arthur Travers Publisher :Springer Science & Business Media ISBN 13 :9780412259906 Total Pages :194 pages Book Rating :4.2/5 (599 download)
Book Synopsis DNA-Protein Interactions by : Andrew Arthur Travers
Download or read book DNA-Protein Interactions written by Andrew Arthur Travers and published by Springer Science & Business Media. This book was released on 1993-04-30 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: The binding of proteins to DNA and the manipulation of DNA by proteins are crucial aspects of the biological role of DNA in the living cell. This book provides a comprehensive and lucid discussion of the molecular interactions involved.
Book Synopsis Transcription by : Ronald C. Conaway
Download or read book Transcription written by Ronald C. Conaway and published by Raven Press (ID). This book was released on 1994 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a coherent account of many productive lines of investigation, organized as a series of mini-reviews that focus on major research areas including studies on the structure and mechanisms of action of bacterial, viral, and eukaryotic RNA polymerases, and the transcription factors that control their activities. Each review provides a brief but up-to-date account of the progress of research in a particular area, a discussion of the major issues and questions driving that research, and a brief description of the evolving approaches and technologies used to address those questions. Annotation copyright by Book News, Inc., Portland, OR
Book Synopsis In Vitro Analysis of Rifampicin Resistant Escherichia Coli RNA Polymerases by : David Edward Solow-Cordero
Download or read book In Vitro Analysis of Rifampicin Resistant Escherichia Coli RNA Polymerases written by David Edward Solow-Cordero and published by . This book was released on 1995 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2068 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Book Synopsis Structural and Functional Characterization of Escherichia Coli RNA Polymerase Ternary Complexes During Transcript Elongation and Termination by : Susan Marie Uptain
Download or read book Structural and Functional Characterization of Escherichia Coli RNA Polymerase Ternary Complexes During Transcript Elongation and Termination written by Susan Marie Uptain and published by . This book was released on 1997 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part A by : Daniel Herschlag
Download or read book Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part A written by Daniel Herschlag and published by Academic Press. This book was released on 2009-11-16 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. - Expert researchers introduce the most advanced technologies and techniques in protein structure and folding - Includes techniques on tiling assays
Book Synopsis The Enzyme Reference by : Daniel L. Purich
Download or read book The Enzyme Reference written by Daniel L. Purich and published by Elsevier. This book was released on 2003-01-04 with total page 940 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this work is to provide a fuller spectrum of information in a single source on enzyme-catalyzed reactions than is currently available in any published reference work or as part of any Internet database. The Enzyme Reference: A Comprehensive Guidebook to Enzyme Nomenclature, Reactions, and Methods includes 20,000 review articles and seminal research papers. Additionally, it provides a novel treatment of so-called ATPase and GTPase reactions to account for the noncovalent substratelike and productlike states of molecular motors, elongation factors, transporters, DNA helicases, G-reulatory proteins, and other energases. - Includes a compendium of over 6,000 enzyme reactions (including enzyme commission numbers, alternative names, substrates, products, alternative substrates, and properties) - Covers over 900 chemical structures of key metabolites and cofactors - Index directs readers to the exact pages for over 9,500 enzyme names
Download or read book Research Awards Index written by and published by . This book was released on 1982 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
