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Immunofluorescence
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Book Synopsis Handbook of Direct Immunofluorescence by : Douglas Fullen
Download or read book Handbook of Direct Immunofluorescence written by Douglas Fullen and published by JP Medical Ltd. This book was released on 2018-03-18 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunofluorescence is a key diagnostic tool in dermatopathology, and essential in the diagnosis of connective tissue diseases, vasculitis and other cutaneous disorders. The need to interpret the results of immunofluorescence testing, and correlate these with histopathological results, is a key skill required not just of dermatopathologists but also, increasingly, of dermatologists who either read the slides themselves or use a pathology lab or academic referral centre. Handbook of Direct Immunofluorescence covers not only day-to-day findings but also less common patterns and rarities, and gives information on important diagnostic pitfalls. Each chapter is dedicated to a specific disease and is introduced by concise text that describes the clinical presentation and pathogenesis: then, multiple images show the range of histopathological and immunofluorescence findings associated with the disease in question. Key points Practical, clinically oriented coverage provides invaluable resource for dermato-, oral and general pathologists, as well as dermatologists Includes invaluable information on technical aspects (specimen procurement, transportation, etc) as well as guidance on interpreting histopathological and immunofluorescence findings Pattern-based approach serves as logical framework for reaching a diagnosis, as well as understanding when to order additional tests and how to recognise nonspecific findings
Book Synopsis Basic Serological Testing by : Rowa Yousef Alhabbab
Download or read book Basic Serological Testing written by Rowa Yousef Alhabbab and published by Springer. This book was released on 2018-05-23 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing.
Download or read book Biobanking written by William H. Yong and published by Humana Press. This book was released on 2018-12-14 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.
Book Synopsis Mayo Clinic Atlas of Immunofluorescence in Dermatology by : Amer N. Kalaaji
Download or read book Mayo Clinic Atlas of Immunofluorescence in Dermatology written by Amer N. Kalaaji and published by CRC Press. This book was released on 2006-03-30 with total page 77 pages. Available in PDF, EPUB and Kindle. Book excerpt: Filling a gap in the literature, this atlas stands as a unique authoritative source to thoroughly review the many immunofluorescence patterns seen in immunodermatology for conditions such as immunobullous diseases, connective tissue diseases, vasculitis, and other miscellaneous dermatologic disorders.
Book Synopsis Manual of Biological Markers of Disease by : W.J. van Venrooij
Download or read book Manual of Biological Markers of Disease written by W.J. van Venrooij and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 711 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive reference work: This looseleaf work is an authoritative compilation of methods for the detection of autoantibodies (Section A: Methods of Autoantibody Detection); the structure, function, and molecular and biochemical concepts of autoantigens (Section B: Autoantigens); and the clinical significance of measuring autoantibodies in patients with rheumatic, connective tissue and autoimmune diseases (Section C: Clinical Significance of Autoantibodies). This unique work brings together all the molecular and medical information - very difficult to retrieve otherwise - in ONE publication. The Editors and contributors are leading experts in the immunological, molecular biological, and clinical fields. The format of this looseleaf publication allows regular updating of data as well as inclusion of new advances in research on autoimmunity. Until now, the work (Basic work including Supplement 1) included Section A, and the larger part of Section B, both in an attractive and robust ringbinder. Audience: By nature and design of this exciting reference work, it is especially aimed at scientists, including immunologists, pathologists and molecular biologists, and clinical chemists, as well as clinicians specializing in rheumatic diseases and autoimmune disorders, inflammation or clinical immunology. Supplement 2: This supplement primarily contains Section C (Clinical Significance of Autoantibodies). As in the other sections, the contents are presented in a consistently structured manner, beautifully illustrated with photos and schematic figures. Extensive literature references are provided. Also, this supplement includes an addition to Section B (Autoantigens), being chapter B.1.5: The Antigens Defined by Antikeratin Antibodies (AKA).
Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Book Synopsis Immunohistochemistry: Basics and Methods by : Igor B. Buchwalow
Download or read book Immunohistochemistry: Basics and Methods written by Igor B. Buchwalow and published by Springer Science & Business Media. This book was released on 2010-01-26 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Book Synopsis Immunohistochemistry and Immunocytochemistry by : Luis Del Valle
Download or read book Immunohistochemistry and Immunocytochemistry written by Luis Del Valle and published by . This book was released on 2022 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field.
Book Synopsis Renal Pathology in Biopsy by : H.U. Zollinger
Download or read book Renal Pathology in Biopsy written by H.U. Zollinger and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vor die Therapie setzten die Gotter die Diagnose. Otto NiigeJi Renal biopsy has decisively enriched renal diagnostics. Kidney diseases may be monitored during their entire course, and new techniques - such as immunofluorescence and electron microscopy - may be systematically applied, resulting in novel insights into the morphogenesis, pathogenesis, and etiology of kidney lesions. These insights, in turn, have served as new starting points, in the spirit of the quotation above, for the institution of causal therapy by the clinician. This work presents our findings based on 20 years of experience in evaluating renal biopsies. As of the end of 1974, our computer-supported, systematic clinical, morphologic, and follow-up evaluation of case material consisted of over 2000 biopsies, including 679 examined by electron microscopy and 400 by immunofluorescence microscopy. The subsequent 500 biopsies (400 studied by electron microscopy and 300 by immunofluorescence) were con sidered qualitatively only. In order to enhance qualitative findings with quantitative data, it was necessary to devise new methods for quantifying electron-microscopic findings. Additionally, we attempted to correlate cyto logic and immunofluorescent observations to integrate the isolated findings of electron microscopy into a vital cytologic pattern of reactions. We also attempted to evaluate the almost overwhelming flood of publications, especially those appearing within the last 10 years. The idea for this book was conceived a decade ago. At that time, however, our own experience in renal biopsy diagnostics seemed insufficient to sup port such a major undertaking.
Book Synopsis Monoclonal Antibodies by : James W. Goding
Download or read book Monoclonal Antibodies written by James W. Goding and published by . This book was released on 1986 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book represents the distillation and critical evaluation of many hundreds of publications relating to the production and use of antibodies. Therefore it is restricted to the "core" techniques of production and handling of antibodies, and their use in studies of antigen analysis, purification and localization.
Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck
Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-03-27 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
Book Synopsis Immunohistology in Diagnostic Pathology by : J. Charles Jennette
Download or read book Immunohistology in Diagnostic Pathology written by J. Charles Jennette and published by CRC Press. This book was released on 1988-11-30 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advantages and disadvantages of each immunostaining technique are discussed in this informative and useful text. Immunofluorescence and immunoenzyme staining procedures, including acquisition of reagents, tissue preparation, quality control and methodological trouble shooting, are highlighted. The use of immunohistology for diagnostic evaluation of Kidney diseases, skin diseases, lymphoproliferative diseases, difficult to diagnose neoplastic diseases, nervous system diseases, and infectious diseases is presented. This book is beneficial for diagnostic anatomic pathologists, and biomedical researchers. Chapters Cover: Advantages and disadvantages of each immunostaining technique Acquisition of reagents Tissue preparation Quality control Methodological trouble shooting
Download or read book Bioimaging written by Douglas E. Chandler and published by Jones & Bartlett Publishers. This book was released on 2009 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
Book Synopsis Plant Microtechniques and Protocols by : Edward Chee Tak Yeung
Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by Springer. This book was released on 2015-09-19 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Book Synopsis A Laboratory Guide to the Tight Junction by : Jianghui Hou
Download or read book A Laboratory Guide to the Tight Junction written by Jianghui Hou and published by Academic Press. This book was released on 2020-04-24 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. - Provides consistent and detailed research methods - Covers various cell, tissue and animal models - Includes step-by-step guidance from beginner to sophisticated levels
Book Synopsis Protein Localization by Fluorescence Microscopy by : Viki Allan
Download or read book Protein Localization by Fluorescence Microscopy written by Viki Allan and published by OUP Oxford. This book was released on 1999-11-18 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.
Book Synopsis Index-catalogue of Medical and Veterinary Zoology by :
Download or read book Index-catalogue of Medical and Veterinary Zoology written by and published by . This book was released on 1975 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt:
