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Imaging Manipulation And Optogenetics In Zebrafish
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Book Synopsis Imaging, Manipulation and Optogenetics in Zebrafish by : Itia Amandine Favre‐Bulle
Download or read book Imaging, Manipulation and Optogenetics in Zebrafish written by Itia Amandine Favre‐Bulle and published by Springer. This book was released on 2018-08-12 with total page 99 pages. Available in PDF, EPUB and Kindle. Book excerpt: The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.
Book Synopsis Imaging, Manipulation and Optogenetics in Zebrafish by : Itia Amandine Favre-Bulle
Download or read book Imaging, Manipulation and Optogenetics in Zebrafish written by Itia Amandine Favre-Bulle and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force - in this case on otoliths (ear stones) - and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.
Book Synopsis Novel Tools for Simultaneous Optogenetic Manipulation and Calcium Imaging in the Zebrafish Nervous System by :
Download or read book Novel Tools for Simultaneous Optogenetic Manipulation and Calcium Imaging in the Zebrafish Nervous System written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Live Imaging In Zebrafish: Insights Into Development And Disease by : Karuna Sampath
Download or read book Live Imaging In Zebrafish: Insights Into Development And Disease written by Karuna Sampath and published by World Scientific. This book was released on 2010-08-30 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: The zebrafish embryo has now been well established as an organism of choice for the genetic and cell biological basis of vertebrate development. Large-scale forward genetic screens together with reverse genetic tools have resulted in a flood of new information on the regulation of vertebrate development. Furthermore, the unique transparency of the zebrafish embryo allows unprecedented access to cells and tissues and many important discoveries on gene function have already been made using live imaging techniques in this organism. This book successfully brings together state-of-the-art methodologies of live imaging, such as the use of fluorescence correlation spectroscopy (FCS), that will be useful for investigators in the field of developmental biology to address their favorite problem using cell biological approaches in the zebrafishOne of the key reasons that has made the zebrafish such a popular vertebrate model is its transparency and its potential for high-resolution cell biology and imaging. However, there is no comprehensive and up-to-date guide to the new “live imaging” tools that can now be used in the zebrafish. Furthermore, there are many new microscopy methods that have been adapted for use in zebrafish, and this book can be a good guide even for scientists already familiar with the imaging advantages of this model.
Book Synopsis Imaging Thoughts - an Investigation of Neural Circuits Encoding Changes in Behavior in Zebrafish by : Claire Sylvie Oldfield
Download or read book Imaging Thoughts - an Investigation of Neural Circuits Encoding Changes in Behavior in Zebrafish written by Claire Sylvie Oldfield and published by . This book was released on 2016 with total page 93 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experience influences how we perceive the world, how we interact with our environment, and how we develop. In fact, almost all animals can modify their behavior as a result of experience. Psychologists have long distinguished between different forms of learning and memory, and later determined that they are encoded in distinct brain areas. Neuroscientists dating back to Santiago Ramón y Cajal suggested that learning and memory might be encoded as changes in synaptic connections between neurons, but it wasn't until the second half of the 20th century that experimental evidence corroborated this idea. Specific firing patterns of neurons during learning results in strengthening or weakening of synapses, and morphological modifications that can lead to long lasting changes in neural circuits. The molecular mechanisms that drive these changes are remarkably conserved across vertebrates. However, understanding how synaptic plasticity is integrated at a network level remains a big challenge in neuroscience. Relatively few studies have focused on how neural circuits encode changes in behavior in a natural context. In this dissertation I present two such examples using calcium imaging and optogenetic techniques in larval zebrafish. First, in collaboration with others, I studied neural activity that drives the onset of the very first behavior of the fish, tail coiling. We characterized the transition of spontaneous activity in the spinal cord from a sporadic, uncorrelated state, to a regular and synchronized state. We determined that this transition occurred through coalescence of local microcircuits, and that inhibiting the early sporadic activity impaired normal development of the central pattern generator network. Second, I studied how experience of prey affects hunting behavior and underlying neural activity. I developed a paradigm to assess the effect of prior hunting on prey capture behavior and showed that experienced fish initiate more captures than their naïve counterparts. I next established that experience does not affect the ability of the fish to see prey. Rather, experience increases the probability that activity in visual areas will evoke a capture initiation. This is accomplished by increasing the impact of information transfer from visual to motor areas, possibly due to increased activity in forebrain areas for experienced fish.
Book Synopsis Whole-brain Voltage Imaging in Larval Zebrafish Using Light-field Microscopy by : Zeguan Wang
Download or read book Whole-brain Voltage Imaging in Larval Zebrafish Using Light-field Microscopy written by Zeguan Wang and published by . This book was released on 2020 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monitoring pan-neuronal electrical activity in awake vertebrates is a yet-to-be-achieved goal in neuroscience that will facilitate our efforts to understand how the brain processes information and mediates behaviors. Here we present Sparse Decomposition Light-Field Microscopy (SDLFM), a light-field microscopy method with improved resolution for volumetric neural activity imaging, and computational and experimental evaluations of its capacity for whole-brain voltage imaging in larval zebrafish. The work in this thesis includes three parts. First, we designed and implemented SDLFM and demonstrated its cellular-resolution ability in larval zebrafish expressing pan-neuronal GCaMP6. Second, we simulated the voltage imaging and the computation processes of SDLFM for zebrafish brains and found that in order to achieve a >0.9 correlation coefficient between the extracted SDLFM signals and the real voltage signals with our current hardware and voltage indicators, the proportion of the neurons randomly labeled with fluorescence in a zebrafish brain must be reduced to below 20%. Finally, we imaged live brains of zebrafish larvae expressing pan-neuronal Voltron525-ST and transient SomArchon at a volume rate of 307 Hz with a volumetric field-of-view of ~700 [mu]mx~400 [mu]mx~300 [mu]m. Although spontaneous spikes were traced in these recordings, we speculate some signals might result from noise and artifacts. In summary, we have developed SDLFM, which allows high-speed whole-brain imaging at cellular resolution in larval zebrafish. New camera sensors with higher quantum efficiency and larger dynamic range, and new voltage indicators with increased brightness and sensitivity are needed to achieve the signal-to-noise ratio required to record the voltage activity from a densely labeled fish brain.
Book Synopsis Robotic Micromanipulation of Zebrafish Larva by : Songlin Zhuang
Download or read book Robotic Micromanipulation of Zebrafish Larva written by Songlin Zhuang and published by Springer. This book was released on 2023-07-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers readers a series of robotic methods for manipulating zebrafish larva, one of the most popular model vertebrates widely used in biomedical research and clinical applications. The authors leverage advanced control theories, image processing algorithms, and artificial intelligence to establish a robot-assisted automated or semi-automated zebrafish larva-targeted micromanipulation system for different experimental purposes. The methods presented are generic and can be translated to manipulate other types of biological objects, such as embryos or cells. Coverage includes topics that span the procedures of manipulating zebrafish larva, such as in-plane positioning, three-dimensional orientation, deformation-controllable immobilization, organ-targeted microinjection, whole-organism imaging, and high-throughput trajectory tracking of zebrafish larvae group movement. Robotic Micromanipulation of Zebrafish Larva is written in a simple, clear, and easy-to-read style. It is an ideal reference for academic researchers and biomedical operators. It is also a valuable resource for students learning robotics, control and system theories, image processing, artificial intelligence, and biomedical engineering.
Download or read book Optogenetics written by Peter Hegemann and published by Walter de Gruyter. This book was released on 2013-08-28 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optogenetics combines genetic engineering with optics to observe and control the function of cells using light, with clinical implications for restoration of vision and treatment of neurological diseases. As a new discipline much of the basic science and methods are currently under investigation and active development, thus there is a strong need for introductory literature in this field. This graduate level textbook provides an overview of the field of optogenetics in 5 concise chapters: Optogenetic tools, Applications in cellular systems, Mapping neuronal networks, Clinical applications and Restoration of vision and hearing. The concept and content was developed with top international researchers and students at a prestigious Dahlem Conference workshop.
Book Synopsis Live cell imaging: Cell and developmental research bridging education, optical engineering, industry, software, shared facilities by : Michelle S. Itano
Download or read book Live cell imaging: Cell and developmental research bridging education, optical engineering, industry, software, shared facilities written by Michelle S. Itano and published by Frontiers Media SA. This book was released on 2023-04-28 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optogenetics written by Hiromu Yawo and published by Springer Nature. This book was released on 2021-01-05 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, now in a thoroughly revised second edition, offers a comprehensive review of the rapidly growing field of optogenetics, in which light-sensing proteins are genetically engineered into cells in order to acquire information on cellular physiology in optical form or to enable control of specific network in the brain upon activation by light. Light-sensing proteins of various living organisms are now available to be exogenously expressed in neurons and other target cells both in vivo and in vitro. Cellular functions can thus be manipulated or probed by light. The new edition documents fully the extensive progress since publication of the first edition to provide an up-to-date overview of the physical, chemical, and biological properties of light-sensing proteins and their application in biological systems, particularly in neuroscience but also in medicine and the optical sciences. Underlying principles are explained and detailed information provided on a wide range of optogenetic tools for the observation and control of cellular signaling and physiology, gene targeting technologies, and optical methods for biological applications. In presenting the current status of optogenetics and emerging directions, this milestone publication will be a “must read” for all involved in research in any way related to optogenetics.
Book Synopsis Genetics of Sleep and Sleep Disorders by : Philip Gehrman
Download or read book Genetics of Sleep and Sleep Disorders written by Philip Gehrman and published by Springer Nature. This book was released on with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optogenetics written by Thomas Knopfel and published by Elsevier. This book was released on 2012-04-19 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optogenetics is a fast-growing field concerning the invention, and use, of molecules that are genetically expressed in cells, and then either report on cellular physiology in optical form, or enable control of specific pathways in cells when actuated by light. This book reviews the current state, as well as the underlying principles and future directions, of a diversity of optogenetic tools of importance for observing and controlling cellular signaling and physiology. These tools are already revolutionizing neuroscience, and are starting to have impact on fields ranging from metabolism to development to cardiology. The book contains a dozen chapters written by world experts on these topics, half focusing on the optogenetic molecular tools themselves, and half on the genetic and hardware approaches that make them powerfully useful in intact tissues and organisms. Leading authors review the state-of-the-art in their field of investigation and provide their views and perspectives for future research Chapters are extensively referenced to provide readers with a comprehensive list of resources on the topics covered All chapters include comprehensive background information and are written in a clear form that is also accessible to the non-specialist
Book Synopsis All-Optical Methods to Study Neuronal Function by : Eirini Papagiakoumou
Download or read book All-Optical Methods to Study Neuronal Function written by Eirini Papagiakoumou and published by Springer Nature. This book was released on 2023-02-20 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this book cover topics over a broad range, from fundamental background information in both physiology and optics in the context of all-optical neurophysiology experiments, to the design principles and hardware implementation of optical methods used for photoactivation and imaging. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, All-Optical Methods to Study Neuronal Function is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors. This is an open access book.
Book Synopsis Cardiac Optogenetics: Using Light to Observe and Excite the Heart by : Tobias Bruegmann
Download or read book Cardiac Optogenetics: Using Light to Observe and Excite the Heart written by Tobias Bruegmann and published by Frontiers Media SA. This book was released on 2022-11-15 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topic Editor Godfrey Smith is the founder, director and honorary Chief Scientific Officer of Clyde Biosciences Ltd (UK). The other Topic Editors declare no competing interests with regard to the Research Topic subject.
Book Synopsis The Zebrafish in Biomedical Research by : Samuel Cartner
Download or read book The Zebrafish in Biomedical Research written by Samuel Cartner and published by Academic Press. This book was released on 2019-11-22 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Zebrafish in Biomedical Research: Biology, Husbandry, Diseases, and Research Applications is a comprehensive work that fulfills a critical need for a thorough compilation of information on this species. The text provides significant updates for working vivarium professionals maintaining zebrafish colonies, veterinarians responsible for their care and well-being, zoologists and ethologists studying the species, and investigators using the species to gain critical insights into human physiology and disease. As the zebrafish has become an important model organism for the study of vertebrate development and disease, organ function, behavior, toxicology, cancer, and drug discovery, this book presents an important resource for future research. Presents a complete view of the zebrafish, covering their biology, husbandry, diseases and research applications Includes the work of world-renowned authors Provides the first authoritative and comprehensive treatment of zebrafish in biomedical research as part of the ACLAM series
Book Synopsis Basic Electrophysiological Methods by : Ellen Covey
Download or read book Basic Electrophysiological Methods written by Ellen Covey and published by Oxford University Press. This book was released on 2015-02-25 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.
Book Synopsis Cancer and Zebrafish by : David M. Langenau
Download or read book Cancer and Zebrafish written by David M. Langenau and published by Springer. This book was released on 2016-05-10 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.
