Hplc Of Peptides And Proteins

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HPLC of Peptides and Proteins

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
ISBN 13 : 1592597424
Total Pages : 411 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis HPLC of Peptides and Proteins by : Marie-Isabel Aguilar

Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

A Practical Guide to Protein and Peptide Purification for Microsequencing

Author : Paul T. Matsudaira
Publisher : Elsevier
ISBN 13 : 0080924611
Total Pages : 205 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis A Practical Guide to Protein and Peptide Purification for Microsequencing by : Paul T. Matsudaira

Download or read book A Practical Guide to Protein and Peptide Purification for Microsequencing written by Paul T. Matsudaira and published by Elsevier. This book was released on 2012-12-02 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing

Peptide Characterization and Application Protocols

Author : Gregg B. Fields
Publisher : Humana Press
ISBN 13 : 9781617376375
Total Pages : 342 pages
Book Rating : 4.3/5 (763 download)

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Book Synopsis Peptide Characterization and Application Protocols by : Gregg B. Fields

Download or read book Peptide Characterization and Application Protocols written by Gregg B. Fields and published by Humana Press. This book was released on 2010-11-19 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.

Total Chemical Synthesis of Proteins

Author : Ashraf Brik
Publisher : John Wiley & Sons
ISBN 13 : 3527346600
Total Pages : 626 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Total Chemical Synthesis of Proteins by : Ashraf Brik

Download or read book Total Chemical Synthesis of Proteins written by Ashraf Brik and published by John Wiley & Sons. This book was released on 2021-06-08 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.

HPLC of Proteins, Peptides and Polynucleotides

Author : Milton T. W. Hearn
Publisher :
ISBN 13 :
Total Pages : 800 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis HPLC of Proteins, Peptides and Polynucleotides by : Milton T. W. Hearn

Download or read book HPLC of Proteins, Peptides and Polynucleotides written by Milton T. W. Hearn and published by . This book was released on 1991 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: HPLC is High Performance Liquid Chromatography.

Preparative Chromatography for Separation of Proteins

Author : Arne Staby
Publisher : John Wiley & Sons
ISBN 13 : 1119031176
Total Pages : 608 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Preparative Chromatography for Separation of Proteins by : Arne Staby

Download or read book Preparative Chromatography for Separation of Proteins written by Arne Staby and published by John Wiley & Sons. This book was released on 2017-02-02 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers

Protein Structure Analysis

Author : Roza Maria Kamp
Publisher : Springer Science & Business Media
ISBN 13 : 3642592198
Total Pages : 311 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Protein Structure Analysis by : Roza Maria Kamp

Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

HPLC for Pharmaceutical Scientists

Author : Yuri V. Kazakevich
Publisher : John Wiley & Sons
ISBN 13 : 0470087943
Total Pages : 1136 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis HPLC for Pharmaceutical Scientists by : Yuri V. Kazakevich

Download or read book HPLC for Pharmaceutical Scientists written by Yuri V. Kazakevich and published by John Wiley & Sons. This book was released on 2007-02-16 with total page 1136 pages. Available in PDF, EPUB and Kindle. Book excerpt: HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.

HPLC in Enzymatic Analysis

Author : Edward F. Rossomando
Publisher : John Wiley & Sons
ISBN 13 : 0470111003
Total Pages : 480 pages
Book Rating : 4.4/5 (71 download)

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Book Synopsis HPLC in Enzymatic Analysis by : Edward F. Rossomando

Download or read book HPLC in Enzymatic Analysis written by Edward F. Rossomando and published by John Wiley & Sons. This book was released on 2009-09-25 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.

Food Analysis by HPLC

Author : Leo M.L. Nollet
Publisher : CRC Press
ISBN 13 : 1439830851
Total Pages : 1078 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Food Analysis by HPLC by : Leo M.L. Nollet

Download or read book Food Analysis by HPLC written by Leo M.L. Nollet and published by CRC Press. This book was released on 2012-11-16 with total page 1078 pages. Available in PDF, EPUB and Kindle. Book excerpt: For food scientists, high-performance liquid chromatography (HPLC) is a powerful tool for product composition testing and assuring product quality. Since the last edition of this volume was published, great strides have been made in HPLC analysis techniques-with particular attention given to miniaturization, automatization, and green chemistry. Tho

Sample Preparation in LC-MS Bioanalysis

Author : Wenkui Li
Publisher : John Wiley & Sons
ISBN 13 : 111927429X
Total Pages : 384 pages
Book Rating : 4.1/5 (192 download)

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Book Synopsis Sample Preparation in LC-MS Bioanalysis by : Wenkui Li

Download or read book Sample Preparation in LC-MS Bioanalysis written by Wenkui Li and published by John Wiley & Sons. This book was released on 2019-03-12 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes) Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor) Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates) Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.

Protein and Peptide Analysis by Mass Spectrometry

Author : J. R. Chapman
Publisher : Springer Science & Business Media
ISBN 13 :
Total Pages : 368 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Protein and Peptide Analysis by Mass Spectrometry by : J. R. Chapman

Download or read book Protein and Peptide Analysis by Mass Spectrometry written by J. R. Chapman and published by Springer Science & Business Media. This book was released on 1996-08-19 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.

Quantitative Structure

Author : Roman Kaliszan
Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 328 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Quantitative Structure by : Roman Kaliszan

Download or read book Quantitative Structure written by Roman Kaliszan and published by Wiley-Interscience. This book was released on 1987-10-14 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of computers in numerical characterization of molecular structures has given chemists fundamentally new information on chemical structures, leading to major developments in physical, analytical, and medicinal chemistry. This book, written by a pioneer in the field, extends and updates research on quantitative structure retention relationships (QSRR) by consolidating and critically reviewing the extensive literature on the subject while providing basic theoretical and practical information required in all investigations involving chromatography, analytical chemistry, biochemistry, and pharmaceutical research. Coverage includes detailed discussions of the general theories and mechanisms of chromatographic separations, prediction of retention coefficients, statistical techniques and formal requirements of QSRR studies, specific applications of chromatographic data, and much more. Also provides several carefully selected figures and tables plus extensive bibliographies.

Applications of HPLC in Biochemistry

Author : A. Fallon
Publisher : Elsevier
ISBN 13 : 0080858864
Total Pages : 355 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Applications of HPLC in Biochemistry by : A. Fallon

Download or read book Applications of HPLC in Biochemistry written by A. Fallon and published by Elsevier. This book was released on 1987-07-01 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.

Hplc Of Biological Macro- Molecules, Revised And Expanded

Author : Karen M. Gooding
Publisher : CRC Press
ISBN 13 : 9780203911594
Total Pages : 806 pages
Book Rating : 4.9/5 (115 download)

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Book Synopsis Hplc Of Biological Macro- Molecules, Revised And Expanded by : Karen M. Gooding

Download or read book Hplc Of Biological Macro- Molecules, Revised And Expanded written by Karen M. Gooding and published by CRC Press. This book was released on 2002-01-08 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

Basic HPLC and CE of Biomolecules

Author : Robert L. Cunico
Publisher : Bay Bioanalytical Laboratory, Incorporated
ISBN 13 : 9780966322903
Total Pages : 0 pages
Book Rating : 4.3/5 (229 download)

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Book Synopsis Basic HPLC and CE of Biomolecules by : Robert L. Cunico

Download or read book Basic HPLC and CE of Biomolecules written by Robert L. Cunico and published by Bay Bioanalytical Laboratory, Incorporated. This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of HPLC

Author : Danilo Corradini
Publisher : CRC Press
ISBN 13 : 1420016946
Total Pages : 718 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Handbook of HPLC by : Danilo Corradini

Download or read book Handbook of HPLC written by Danilo Corradini and published by CRC Press. This book was released on 2016-04-19 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the