Atomic Force Microscopy in Liquid

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Publisher : John Wiley & Sons
ISBN 13 : 3527327584
Total Pages : 385 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró

Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-05-14 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Encyclopedia of Biophysics

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Publisher : Springer
ISBN 13 : 9783642167119
Total Pages : 0 pages
Book Rating : 4.1/5 (671 download)

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Book Synopsis Encyclopedia of Biophysics by : Gordon Roberts

Download or read book Encyclopedia of Biophysics written by Gordon Roberts and published by Springer. This book was released on 2012-10-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia of Biophysics is envisioned both as an easily accessible source of information and as an introductory guide to the scientific literature. It includes entries describing both Techniques and Systems. In the Techniques entries, each of the wide range of methods which fall under the heading of Biophysics are explained in detail, together with the value and the limitations of the information each provides. Techniques covered range from diffraction (X-ray, electron and neutron) through a wide range of spectroscopic methods (X-ray, optical, EPR, NMR) to imaging (from electron microscopy to live cell imaging and MRI), as well as computational and simulation approaches. In the Systems entries, biophysical approaches to specific biological systems or problems – from protein and nucleic acid structure to membranes, ion channels and receptors – are described. These sections, which place emphasis on the integration of the different techniques, therefore provide an inroad into Biophysics from a biological more than from a technique-oriented physical/chemical perspective. Thus the Encyclopedia is intended to provide a resource both for biophysicists interested in methods beyond those used in their immediate sub-discipline and for those readers who are approaching biophysics from either a physical or biological background.

High-Speed Atomic Force Microscopy in Biology

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Author :
Publisher : Springer Nature
ISBN 13 : 3662647850
Total Pages : 327 pages
Book Rating : 4.6/5 (626 download)

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Book Synopsis High-Speed Atomic Force Microscopy in Biology by : Toshio Ando

Download or read book High-Speed Atomic Force Microscopy in Biology written by Toshio Ando and published by Springer Nature. This book was released on 2022-03-23 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Numerous examples of HS-AFM studies on proteins demonstrate how to apply this new form of microscopy to specific biological problems. Several precautions for successful imaging and the preparation of cantilever tips and substrate surfaces will greatly benefit first-time users of HS-AFM. In turn, the instrumentation techniques detailed in Chapter 4 can be skipped, but will be useful for engineers and scientists who want to develop the next generation of high-speed scanning probe microscopes for biology. The book is intended to facilitate the first-time use of this new technique, and to inspire students and researchers to tackle their own specific biological problems by directly observing dynamic events occurring in the nanoscopic world. Microscopy in biology has recently entered a new era with the advent of high-speed atomic force microscopy (HS-AFM). Unlike optical microscopy, electron microscopy, and conventional slow AFM, it allows us to directly observe biological molecules in physiological environments. Molecular “movies” created using HS-AFM can directly reveal how molecules behave and operate, without the need for subsequent complex analyses and roundabout interpretations. It also allows us to directly monitor morphological change in live cells, and dynamic molecular events occurring on the surfaces of living bacteria and intracellular organelles. As HS-AFM instruments were recently commercialized, in the near future HS-AFM is expected to become a common tool in biology, and will enhance and accelerate our understanding of biological phenomena.

Biophysical Analysis of Membrane Proteins

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Author :
Publisher : Wiley-Blackwell
ISBN 13 :
Total Pages : 376 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Biophysical Analysis of Membrane Proteins by : Eva Pebay-Peyroula

Download or read book Biophysical Analysis of Membrane Proteins written by Eva Pebay-Peyroula and published by Wiley-Blackwell. This book was released on 2008 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.

Noncontact Atomic Force Microscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 9783540431176
Total Pages : 468 pages
Book Rating : 4.4/5 (311 download)

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Book Synopsis Noncontact Atomic Force Microscopy by : S. Morita

Download or read book Noncontact Atomic Force Microscopy written by S. Morita and published by Springer Science & Business Media. This book was released on 2002-07-24 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since 1995, the noncontact atomic force microscope (NC-AFM) has achieved remarkable progress. Based on nanomechanical methods, the NC-AFM detects the weak attractive force between the tip of a cantilever and a sample surface. This method has the following characteristics: it has true atomic resolution; it can measure atomic force interactions, i.e. it can be used in so-called atomic force spectroscopy (AFS); it can also be used to study insulators; and it can measure mechanical responses such as elastic deformation. This is the first book that deals with all of the emerging NC-AFM issues.

Atomic Force Microscopy

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Author :
Publisher : Humana Press
ISBN 13 : 9781493988938
Total Pages : 372 pages
Book Rating : 4.9/5 (889 download)

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Book Synopsis Atomic Force Microscopy by : Nuno C. Santos

Download or read book Atomic Force Microscopy written by Nuno C. Santos and published by Humana Press. This book was released on 2018-10-30 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

Atomic Force Microscopy in Liquid

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527649824
Total Pages : 385 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró

Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-08-01 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Single Molecule Dynamics in Life Science

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Publisher : John Wiley & Sons
ISBN 13 : 3527312889
Total Pages : 347 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Single Molecule Dynamics in Life Science by : Toshio Yanagida

Download or read book Single Molecule Dynamics in Life Science written by Toshio Yanagida and published by John Wiley & Sons. This book was released on 2008-11-24 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Atomic Force Microscopy in Nanobiology

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Author :
Publisher : CRC Press
ISBN 13 : 9814411590
Total Pages : 444 pages
Book Rating : 4.8/5 (144 download)

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Book Synopsis Atomic Force Microscopy in Nanobiology by : Kunio Takeyasu

Download or read book Atomic Force Microscopy in Nanobiology written by Kunio Takeyasu and published by CRC Press. This book was released on 2016-04-19 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in atomic force microscopy (AFM) have been accomplished through various technical and instrumental innovations, including high-resolution and recognition imaging technology under physiological conditions, fast-scanning AFM, and general methods for cantilever modification and force measurement. All these techniques are now highly

Atomic Force Microscopy

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Author :
Publisher : Oxford University Press
ISBN 13 : 0199570450
Total Pages : 257 pages
Book Rating : 4.1/5 (995 download)

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Book Synopsis Atomic Force Microscopy by : Peter Eaton

Download or read book Atomic Force Microscopy written by Peter Eaton and published by Oxford University Press. This book was released on 2010-03-25 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopes are very important tools for the advancement of science and technology. This book provides an introduction to the microscopes so that scientists and engineers can learn both how to use them, and what they can do.

Atomic Force Microscopy

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1592596479
Total Pages : 388 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Atomic Force Microscopy by : Pier Carlo Braga

Download or read book Atomic Force Microscopy written by Pier Carlo Braga and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).

Control System Design for High-Speed Atomic Force Microscopy

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Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (131 download)

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Book Synopsis Control System Design for High-Speed Atomic Force Microscopy by : Nastaran Nikooienejad

Download or read book Control System Design for High-Speed Atomic Force Microscopy written by Nastaran Nikooienejad and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Video-rate atomic force microscopy is in high demand to visualize dynamic processes in realtime, whereas the functionality of a commercial atomic force microscope (AFM) is restricted to low-speed scans. In the past decades, extensive research efforts have aimed to reinforce the AFM structure toward high-speed atomic force microscopy. However, video-rate imaging of a relatively large scan area is still challenging due to the highly resonant nature of AFM and its conventional method of scanning. The AFM control system also needs to be significantly improved to harness the full potential of AFM mechanical structure at video rate and provide adequate robustness during scan. Therefore, besides the AFM configuration, scanning methods and control techniques contribute significantly to achieving the ultimate goal of sequential AFM imaging at video rate. This dissertation focuses on novel scanning methods and control design methodologies that facilitate sequential atomic force microscopy and improve positioning accuracy at high speed. First, we leverage a technique to smoothen the sequential cycloid trajectory and mitigate the sudden back and forth motions of a positioner in capturing successive frames of a scan. The resulting trajectory reduces the residual tracking error and enhances the AFM image quality. We also propose a systematic design methodology for a novel repetitive non-raster scan trajectory based on the rosette pattern. This pattern, generated by pure harmonic waveforms, can address the conventional issues with sequential non-raster imaging by enabling a smooth and continuous scan. We provide a thorvii ough mathematical analysis of the rosette pattern and a step-by-step design procedure for rosette scanning. We proceed by proposing high-precision model-based control design approaches for sequential AFM imaging using a microelectromechanical system (MEMS) nanopositioner. To precisely follow the reference setpoints in sequential cycloid and rosette scans, we design a tracking controller based on the internal model principle. The internal-model-based controller (IMBC) is intuitive and well-suited for tracking non-raster scan patterns. The controller incorporates the fundamental reference frequencies and their corresponding higher harmonics to reduce the deterministic error originated from uncompensated nonlinearities in the system. However, the resulting controller is of high order, and requires a priori knowledge of the dominant harmonics in the experimental tracking error. To resolve this, we develop a novel control scheme involving an internal-model-based control in feedback and an iterative learning control in feedforward. The internal-model-based controller only includes fundamental frequencies of the references while the iterative learning controller rejects the induced higher harmonics by learning from past experiences. The proposed control scheme is employed for tracking the rosette pattern at various scan rates. We investigate the performance of the proposed scanning methods and control techniques in closed-loop experiments. Finally, a series of high-quality images are obtained at high speed using a MEMS nanopositioner and a commercial AFM. A limiting factor toward high-speed atomic force microscopy is the lightly damped nature of the scanners. To increase the imaging bandwidth and scan speed, vibration control techniques have been practiced. Among them, fixed-structure strictly negative imaginary (SNI) controllers ensure robust stability of closed-loop system when the scanner incorporates collocated actuator and sensor pairs. In the third section of this dissertation, we present a convex synthesis of SNI controllers for a class of multi-input multi-output (MIMO) plants satisfying the negative imaginary property. The design procedure is based on the frequency response data of the plant, and the control objective is to minimize the distance between a desired and actual closed-loop frequency response. The controllers are experimentally implemented to augment damping to the fundamental resonant mode of a MEMS nanopositioner in a two-input two-output configuration.

Myosins

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Publisher : Springer Science & Business Media
ISBN 13 : 1402065191
Total Pages : 498 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Myosins by : Lynne M. Coluccio

Download or read book Myosins written by Lynne M. Coluccio and published by Springer Science & Business Media. This book was released on 2007-11-15 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly authoritative volume highlights the remarkable superfamily of molecular motors called myosins, which are involved in such diverse cellular functions as muscle contraction, intracellular transport, cell migration and cell division. In a timely compilation of chapters written by leading research groups that have made key discoveries in the field, the current understanding of the molecular mechanisms and biological functions of these intriguing proteins is explored.

Atomic Force Microscopy in Molecular and Cell Biology

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Author :
Publisher : Springer
ISBN 13 : 9811315108
Total Pages : 235 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Atomic Force Microscopy in Molecular and Cell Biology by : Jiye Cai

Download or read book Atomic Force Microscopy in Molecular and Cell Biology written by Jiye Cai and published by Springer. This book was released on 2018-11-03 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book addresses new achievements in AFM instruments – e.g. higher speed and higher resolution – and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc.

Nanoscale Imaging

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Publisher : Humana Press
ISBN 13 : 9781493985906
Total Pages : 600 pages
Book Rating : 4.9/5 (859 download)

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Book Synopsis Nanoscale Imaging by : Yuri L. Lyubchenko

Download or read book Nanoscale Imaging written by Yuri L. Lyubchenko and published by Humana Press. This book was released on 2018-06-29 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.

Cell Imaging Techniques

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Publisher : Springer Science & Business Media
ISBN 13 : 1592599931
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Cell Imaging Techniques by : Douglas J. Taatjes

Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Life at the Nanoscale

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Author :
Publisher : CRC Press
ISBN 13 : 981426797X
Total Pages : 460 pages
Book Rating : 4.8/5 (142 download)

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Book Synopsis Life at the Nanoscale by : Yves Dufrene

Download or read book Life at the Nanoscale written by Yves Dufrene and published by CRC Press. This book was released on 2019-03-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.