Handbook Of Green Analytical Chemistry

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Handbook of Green Analytical Chemistry

Author : Miguel de la Guardia
Publisher : John Wiley & Sons
ISBN 13 : 111994094X
Total Pages : 569 pages
Book Rating : 4.1/5 (199 download)

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Book Synopsis Handbook of Green Analytical Chemistry by : Miguel de la Guardia

Download or read book Handbook of Green Analytical Chemistry written by Miguel de la Guardia and published by John Wiley & Sons. This book was released on 2012-02-23 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emerging field of green analytical chemistry is concerned with the development of analytical procedures that minimize consumption of hazardous reagents and solvents, and maximize safety for operators and the environment. In recent years there have been significant developments in methodological and technological tools to prevent and reduce the deleterious effects of analytical activities; key strategies include recycling, replacement, reduction and detoxification of reagents and solvents. The Handbook of Green Analytical Chemistry provides a comprehensive overview of the present state and recent developments in green chemical analysis. A series of detailed chapters, written by international specialists in the field, discuss the fundamental principles of green analytical chemistry and present a catalogue of tools for developing environmentally friendly analytical techniques. Topics covered include: Concepts: Fundamental principles, education, laboratory experiments and publication in green analytical chemistry. The Analytical Process: Green sampling techniques and sample preparation, direct analysis of samples, green methods for capillary electrophoresis, chromatography, atomic spectroscopy, solid phase molecular spectroscopy, derivative molecular spectroscopy and electroanalytical methods. Strategies: Energy saving, automation, miniaturization and photocatalytic treatment of laboratory wastes. Fields of Application: Green bioanalytical chemistry, biodiagnostics, environmental analysis and industrial analysis. This advanced handbook is a practical resource for experienced analytical chemists who are interested in implementing green approaches in their work.

Green Analytical Chemistry

Author : Mihkel Koel
Publisher : Royal Society of Chemistry
ISBN 13 : 1788017560
Total Pages : 350 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Green Analytical Chemistry by : Mihkel Koel

Download or read book Green Analytical Chemistry written by Mihkel Koel and published by Royal Society of Chemistry. This book was released on 2019-03-13 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical analysis requires solvents, reagents and energy and generates waste. The main goal of green analytical chemistry is to avoid or reduce the undesirable environmental side effects of chemical analysis, while preserving the classic analytical parameters of accuracy, sensitivity, selectivity and precision. This book portrays the current and changing situation concerning adoption of the principles of green chemistry as applied to analysis. It begins by looking at the advantages of and problems associated with on-site analysis and how analytical techniques can lead to increased productivity, efficiency and accuracy, and thereby reduce the consumption of materials. It then focuses on sample preparation techniques minimising solvent consumption or using alternative solvents, concepts and methods of improving the ‘greenness’ of instrumental analysis where miniaturization is an important part, separation methods from the perspective of green analytical chemistry and chemometrics approaches, which can reduce or can even remove the need for conventional steps in chemical analysis. Aimed at graduates and novices just entering the field, managers of analytical research laboratories, teachers of analytical chemistry and green public policy makers, this title will be a useful addition to any analytical scientist's library.

Green Analytical Chemistry

Author : Miguel de la Guardia
Publisher : Elsevier
ISBN 13 : 9780444537102
Total Pages : 268 pages
Book Rating : 4.5/5 (371 download)

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Book Synopsis Green Analytical Chemistry by : Miguel de la Guardia

Download or read book Green Analytical Chemistry written by Miguel de la Guardia and published by Elsevier. This book was released on 2010-10-27 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides basic coverage of the fundamentals and principles of green chemistry as it applies to chemical analysis. The main goal of Green Analytical Chemistry is to avoid or reduce the undesirable environmental side effects of chemical analysis, while preserving the classic analytical parameters of accuracy, sensitivity, selectivity, and precision. The authors review the main strategies for greening analytical methods, concentrating on minimizing sample preparation and handling, reducing solvent and reagent consumption, reducing energy consumption, minimizing of waste, operator safety and the economic savings that this approach offers. Suggestions are made to educators and editors to standardize terminology in order to facilitate the identification of analytical studies on green alternatives in the literature because there is not a wide and generalized use of a common term that can group efforts to prevent waste, avoid the use of potentially toxic reagents or solvents and those involving the decontamination of wastes. provides environmentally-friendly alternatives to established analytical practice focuses on the cost-saving opportunities offered emphasis on laboratory personnel safety

Green Analytical Chemistry

Author : Justyna Płotka-Wasylka
Publisher : Springer
ISBN 13 : 981139105X
Total Pages : 449 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Green Analytical Chemistry by : Justyna Płotka-Wasylka

Download or read book Green Analytical Chemistry written by Justyna Płotka-Wasylka and published by Springer. This book was released on 2019-08-02 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book explains the principles and fundamentals of Green Analytical Chemistry (GAC) and highlights the current developments and future potential of the analytical green chemistry-oriented applications of various solutions. The book consists of sixteen chapters, including the history and milestones of GAC; issues related to teaching of green analytical chemistry and greening the university laboratories; evaluation of impact of analytical activities on the environmental and human health, direct techniques of detection, identification and determination of trace constituents; new achievements in the field of extraction of trace analytes from samples characterized by complex composition of the matrix; “green” nature of the derivatization process in analytical chemistry; passive techniques of sampling of analytes; green sorption materials used in analytical procedures; new types of solvents in the field of analytical chemistry. In addition green chromatography and related techniques, fast tests for assessment of the wide spectrum of pollutants in the different types of the medium, remote monitoring of environmental pollutants, qualitative and comparative evaluation, quantitative assessment, and future trends and perspectives are discussed. This book appeals to a wide readership of the academic and industrial researchers. In addition, it can be used in the classroom for undergraduate and graduate Ph.D. students focusing on elaboration of new analytical procedures for organic and inorganic compounds determination in different kinds of samples characterized by complex matrices composition.Jacek Namieśnik was a Professor at the Department of Analytical Chemistry, Gdańsk University of Technology, Poland. Justyna Płotka-Wasylka is a teacher and researcher at the same department.

Green Approaches for Chemical Analysis

Author : Emanuela Gionfriddo
Publisher : Elsevier
ISBN 13 : 0128222352
Total Pages : 300 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Green Approaches for Chemical Analysis by : Emanuela Gionfriddo

Download or read book Green Approaches for Chemical Analysis written by Emanuela Gionfriddo and published by Elsevier. This book was released on 2022-09-22 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Green Approaches for Chemical Analysis addresses emerging trends and technologies for the development of green analytical methods. The book covers basic principles of Green Analytical Chemistry (GAC) and describes the most up-to-date strategies used in areas such as sample preparation, instrumental analysis, and use and synthesis of green solvents and sorbents for separation. Many applications of analytical methods are discussed from a “green perspective, such as multiresidue analysis, metabolomics, food analysis, environmental monitoring, and bio-clinical applications. Written by experts in their fields, the book's chapters offer a variety of green analytical solutions readers can apply to their own analytical needs. Combines an overview of the fundamental principles of Green Analytical Chemistry with applications in many various fields of research, including food, the environment and bioanalysis Gives a critical overview of current analytical strategies and the applicability of green alternatives for various analytical purposes, comparing the efficacy of these approaches Clarifies the link between analytical sample preparation and other methods

Challenges in Green Analytical Chemistry

Author : Salvador Garrigues
Publisher : Royal Society of Chemistry
ISBN 13 : 1788015371
Total Pages : 398 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Challenges in Green Analytical Chemistry by : Salvador Garrigues

Download or read book Challenges in Green Analytical Chemistry written by Salvador Garrigues and published by Royal Society of Chemistry. This book was released on 2020-05-13 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a key area of chemistry, improving the greenness of analytical techniques is of great interest to researchers. The last decade has seen some significant developments in this area, including the use of new smart materials as analytical tools. Covering topics including solvent selection, miniaturization and metrics for the evaluation of "greenness" this book will be of use to researchers, both in academia and in industry, interested in integrating safer and more sustainable analytical techniques into their work.

Handbook of Smart Materials in Analytical Chemistry

Author : Miguel de la Guardia
Publisher : John Wiley & Sons
ISBN 13 : 1119422612
Total Pages : 931 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Handbook of Smart Materials in Analytical Chemistry by : Miguel de la Guardia

Download or read book Handbook of Smart Materials in Analytical Chemistry written by Miguel de la Guardia and published by John Wiley & Sons. This book was released on 2019-01-22 with total page 931 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to smart materials and how they are used in sample preparation, analytical processes, and applications This comprehensive, two-volume handbook provides detailed information on the present state of new materials tailored for selective sample preparation and the legal frame and environmental side effects of the use of smart materials for sample preparation in analytical chemistry, as well as their use in the analytical processes and applications. It covers both methodological and applied analytical aspects, relating to the development and application of new materials for solid-phase extraction (SPE) and solid-phase microextraction (SPME), their use in the different steps and techniques of the analytical process, and their application in specific fields such as water, food, air, pharmaceuticals, clinical sciences and forensics. Every chapter in Handbook of Smart Materials in Analytical Chemistry is written by experts in the field to provide a comprehensive picture of the present state of this key area of analytical sciences and to summarize current applications and research literature in a critical way. Volume 1 covers New Materials for Sample Preparation and Analysis. Volume 2 handles Analytical Processes and Applications. Focuses on the development and applications of smart materials in analytical chemistry Covers both, methodological and applied analytical aspects, for the development of new materials and their use in the different steps and techniques of the analytical process and their application in specific fields Features applications in key areas including water, air, environment, pharma, food, forensic, and clinical Presents the available tools for the use of new materials suitable to aid recognition process to the sample preparation and analysis A key resource for analytical chemists, applied laboratories, and instrument companies Handbook of Smart Materials in Analytical Chemistry, 2V Set is an excellent reference book for specialists and advanced students in the areas of analytical chemistry, including both research and application environments.

Green Chemical Analysis and Sample Preparations

Author : Mahmoud H. El-Maghrabey
Publisher : Springer Nature
ISBN 13 : 3030965341
Total Pages : 553 pages
Book Rating : 4.0/5 (39 download)

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Book Synopsis Green Chemical Analysis and Sample Preparations by : Mahmoud H. El-Maghrabey

Download or read book Green Chemical Analysis and Sample Preparations written by Mahmoud H. El-Maghrabey and published by Springer Nature. This book was released on 2022-06-20 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the most recent trends for greening analytical activities beginning with an introduction to green analytical chemistry followed by a discussion of green analytical chemistry metrics and life-cycle assessment approach to analytical method development. The chapters discuss two main topics; first is the most recent techniques for greening sample pretreatment steps, and second is modern trends for tailoring analytical techniques and instrumentation to implement the green analytical chemistry concept. The role of different kinds of green solvents, such as ionic liquids, supercritical fluids, deep eutectic solvents, bio-based solvents, and surfactants, as well as nanomaterials and green sorption materials in greening sample extraction steps is also a focus of this book. Furthermore, different approaches for greening chromatography as a key analytical technique are discussed. The applications of nanomaterials in analytical procedures are deeply reviewed, and miniaturization of spectrometers is also discussed as a recently evolved approach for efficient green on-site analysis. This book will appeal to a wide readership of academic and industrial researchers in different fields. It can be used in the classroom for undergraduate and postgraduate students focusing on the development of new analytical procedures for organic and inorganic compounds determination in different kinds of samples characterized by complex matrices composition. The book will also be useful for researchers that are interested in both chemical analysis and environment protection.

Handbook on Metals in Clinical and Analytical Chemistry

Author : Hans Seiler
Publisher : CRC Press
ISBN 13 : 9780824790943
Total Pages : 778 pages
Book Rating : 4.7/5 (99 download)

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Book Synopsis Handbook on Metals in Clinical and Analytical Chemistry by : Hans Seiler

Download or read book Handbook on Metals in Clinical and Analytical Chemistry written by Hans Seiler and published by CRC Press. This book was released on 1994-01-25 with total page 778 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes general aspects of metals in clinical chemistry focusing not only on the physiology of metal ions and their analytical determination in biological materials, but also on their geochemical distribution, technical uses and environmental effects.

Handbook of Green Analytical Chemistry

Author : Miguel de la Guardia
Publisher : John Wiley & Sons
ISBN 13 : 0470972017
Total Pages : 569 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Handbook of Green Analytical Chemistry by : Miguel de la Guardia

Download or read book Handbook of Green Analytical Chemistry written by Miguel de la Guardia and published by John Wiley & Sons. This book was released on 2012-04-23 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emerging field of green analytical chemistry is concerned with the development of analytical procedures that minimize consumption of hazardous reagents and solvents, and maximize safety for operators and the environment. In recent years there have been significant developments in methodological and technological tools to prevent and reduce the deleterious effects of analytical activities; key strategies include recycling, replacement, reduction and detoxification of reagents and solvents. The Handbook of Green Analytical Chemistry provides a comprehensive overview of the present state and recent developments in green chemical analysis. A series of detailed chapters, written by international specialists in the field, discuss the fundamental principles of green analytical chemistry and present a catalogue of tools for developing environmentally friendly analytical techniques. Topics covered include: Concepts: Fundamental principles, education, laboratory experiments and publication in green analytical chemistry. The Analytical Process: Green sampling techniques and sample preparation, direct analysis of samples, green methods for capillary electrophoresis, chromatography, atomic spectroscopy, solid phase molecular spectroscopy, derivative molecular spectroscopy and electroanalytical methods. Strategies: Energy saving, automation, miniaturization and photocatalytic treatment of laboratory wastes. Fields of Application: Green bioanalytical chemistry, biodiagnostics, environmental analysis and industrial analysis. This advanced handbook is a practical resource for experienced analytical chemists who are interested in implementing green approaches in their work.

Handbook of Environmental Analysis

Author : Pradyot Patnaik
Publisher : CRC Press
ISBN 13 : 9781420065824
Total Pages : 824 pages
Book Rating : 4.0/5 (658 download)

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Book Synopsis Handbook of Environmental Analysis by : Pradyot Patnaik

Download or read book Handbook of Environmental Analysis written by Pradyot Patnaik and published by CRC Press. This book was released on 2010-05-21 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: A reflection of the myriad changes in the field of environmental analysis and the emergence of many new classes of pollutants in recent years, the second edition of Handbook of Environmental Analysis: Chemical Pollutants in Air, Water, Soil, and Solid Wastes covers all aspects of environmental analysis. Completely revised and updated to include new analytical techniques as well as additional chemical structures and reactions, this second edition retains the features — clarity of prose, pertinent examples, and authoritative coverage of a wide range of toxic pollutants — that made the first edition a bestseller. New and updated information in the Second Edition: Chapters on emerging pollutants such as pharmaceuticals, household products, nonionic surfactants, steroids, hormones, flame-retardants, and plasticizers Chapters on oxyhalides, glyphosate herbicides, oil and grease, disinfection by-products, and haloacetic acids A chapter on radioactivity Updated NIOSH methods on air analysis Revised content on gas chromatography and mass spectrometry US EPA and Standard Methods The book provides information on an array of topics from instrumentations, analytical techniques, and sample preparations to statistical calculations, chemical structures, and equations. It includes information on many alternative analytical procedures, making this edition more informative and versatile than its predecessor. It presents the tools and techniques required to measure a wide range of toxic pollutants in our environment.

Handbook of Green Chemistry

Author : Paul T. Anastas
Publisher :
ISBN 13 : 9783527314041
Total Pages : 0 pages
Book Rating : 4.3/5 (14 download)

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Book Synopsis Handbook of Green Chemistry by : Paul T. Anastas

Download or read book Handbook of Green Chemistry written by Paul T. Anastas and published by . This book was released on 2013-02-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited by the inventor of the 12 principles of Green Chemistry, Paul Anastas, the complete 12-volumes of Handbook of Green Chemistry will provide a one-stop resource covering green catalysis, green solvents, green products and green processes. Handbook of Green Chemistry covers highly topical areas in green chemistry such as feedstocks, green chemical engineering, green catalysis (homogeneous, heterogeneous and biocatalysis), separation techniques and solvents like supercritical fluids, ionic liquids and reactions in water. It covers the big environmental and product design issues faced by chemists such as how to make nanoscience greener, design safer, sustainable and less toxic chemicals and make chemical synthesis a greener and more sustainable process. In the final 3 volumes, Handbook of Green Chemistry will cover green products, the chemical engineering behind their processing and what makes a green product, vital in now this is key selling point for industry. Handbook of Green Chemistry publishes in four sets of three volumes. The first three sets are available to purchase now: Handbook of Green Chemistry: Green Catalysis Paul T. Anastas (Series Editor), Robert H. Crabtree (Editor) ISBN: 978-3-527-31577-2 Hardcover | 1082 pages | January 2009 Handbook of Green Chemistry: Green Solvents Paul T. Anastas (Series Editor), Walter Leitner (Editor), Philip G. Jessop (Editor), Chao-Jun Li (Editor), Peter Wasserscheid (Editor), Annegret Stark (Editor) ISBN: 978-3-527-31574-1 Hardcover | 1412 pages | April 2010 Handbook of Green Chemistry: Green Processes Paul T. Anastas (Series Editor), Chao-Jun Li (Volume Editor) Hardcover | 1300 pages | April 2012 ISBN: 978-3-527-31576-5 The remaining set, Handbook of Green Chemistry: Green Products , will publish in May 2015. Introductory Offer! Order the full Hanbook of Green Chemistry, 12 Volume Set before 31st August 2015 and take advantage of the special introductory price as listed at the top of this webpage. Prices will revert to £1605.00/€1890.00/$2720.00 thereafter.

Tools for Green Chemistry

Author : Evan S. Beach
Publisher : John Wiley & Sons
ISBN 13 : 3527695680
Total Pages : 425 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Tools for Green Chemistry by : Evan S. Beach

Download or read book Tools for Green Chemistry written by Evan S. Beach and published by John Wiley & Sons. This book was released on 2017-06-12 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 10 in the Handbook of Green Chemistry series provides useful and practical tools, databases, and laboratory approaches to support chemists working in both academia and industry in achieving their green chemistry goals. Among many other helpful techniques covered, the authors offer prediction software, life cycle assessment methodology, and screening tools.

Analytical Chemistry of Foods

Author : C.S. James
Publisher : Springer Science & Business Media
ISBN 13 : 1461521653
Total Pages : 180 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Analytical Chemistry of Foods by : C.S. James

Download or read book Analytical Chemistry of Foods written by C.S. James and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food laws were fIrst introduced in 1860 when an Act for Preventing the Adulteration of Articles of Food or Drink was passed in the UK. This was followed by the Sale of Food Act in 1875, also in the UK, and later, in the USA, by the Food and Drugs Act of 1906. These early laws were basically designed to protect consumers against unscrupulous adulteration of foods and to safeguard consumers against the use of chemical preservatives potentially harmful to health. Subsequent laws, introduced over the course of the ensuing century by various countries and organisations, have encompassed the features of the early laws but have been far wider reaching to include legislation relating to, for example, specifIc food products, specifIc ingredients and specifIc uses. Conforming to the requirements set out in many of these laws and guidelines requires the chemical and physical analysis of foods. This may involve qualitative analysis in the detection of illegal food components such as certain colourings or, more commonly, the quantitative estimation of both major and minor food constituents. This quantitative analysis of foods plays an important role not only in obtaining the required information for the purposes of nutritional labelling but also in ensuring that foods conform to desired flavour and texture quality attributes. This book outlines the range oftechniques available to the food analyst and the theories underlying the more commonly used analytical methods in food studies.

Sample Preparation Techniques in Analytical Chemistry

Author : Somenath Mitra
Publisher : John Wiley & Sons
ISBN 13 : 0471457825
Total Pages : 480 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Sample Preparation Techniques in Analytical Chemistry by : Somenath Mitra

Download or read book Sample Preparation Techniques in Analytical Chemistry written by Somenath Mitra and published by John Wiley & Sons. This book was released on 2004-04-07 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences.

Handbook of Green Chemistry

Author : Paul T. Anastas
Publisher :
ISBN 13 : 9783527314041
Total Pages : pages
Book Rating : 4.3/5 (14 download)

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Book Synopsis Handbook of Green Chemistry by : Paul T. Anastas

Download or read book Handbook of Green Chemistry written by Paul T. Anastas and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of Water Analysis, Third Edition

Author : Leo M.L. Nollet
Publisher : CRC Press
ISBN 13 : 1439889643
Total Pages : 999 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Handbook of Water Analysis, Third Edition by : Leo M.L. Nollet

Download or read book Handbook of Water Analysis, Third Edition written by Leo M.L. Nollet and published by CRC Press. This book was released on 2013-07-29 with total page 999 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensively revised and updated, Handbook of Water Analysis, Third Edition provides current analytical techniques for detecting various compounds in water samples. Maintaining the detailed and accessible style of the previous editions, this third edition demonstrates water sampling and preservation methods by enumerating different ways to measure chemical and radiological characteristics. It gives step-by-step descriptions of separation, residue determination, and clean-up techniques. See What’s New in the Second Edition: Includes five new chapters covering ammonia, nitrates, nitrites, and petroleum hydrocarbons, as well as organoleptical and algal analysis methodology Compares older methods still frequently used with recently developed protocols, and examines future trends Features a new section regarding organoleptical analysis of water acknowledging that ultimately the consumers of drinking water have the final vote over its quality with respect to odor, flavor, and color The book covers the physical, chemical, and other relevant properties of various substances found in water. It then describes the sampling, cleanup, extraction, and derivatization procedures, and concludes with detection methods. Illustrated with procedure flow charts and schematics, the text includes numerous tables categorizing methods according to type of component, origin of the water sample, parameters and procedures used, and application range. With contributions from international experts, the book guides you through the entire scientific investigation starting with a sampling strategy designed to capture the real-world situation as closely as possible, and ending with an adequate chemometrical and statistical treatment of the acquired data. By organizing data into more than 300 tables, graphs, and charts, and supplementing the text with equations and illustrations, the editors distill a wealth of knowledge into a single accessible reference.