Handbook Of Electroporation

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Handbook of Electroporation

Author : Damijan Miklavčič
Publisher : Springer
ISBN 13 : 9783319328850
Total Pages : 0 pages
Book Rating : 4.3/5 (288 download)

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Book Synopsis Handbook of Electroporation by : Damijan Miklavčič

Download or read book Handbook of Electroporation written by Damijan Miklavčič and published by Springer. This book was released on 2017-09-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of 156 chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The Handbook's 276 authors have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook. The book was edited by an international team of Section Editors: P. Thomas Vernier, Boris Rubinsky, Juergen Kolb, Damijan Miklavcic, Marie-Pierre Rols, Javier Raso, Richard Heller, Gregor Serša, Dietrich Knorr, and Eugene Vorobiev.

Electroporation and Electrofusion in Cell Biology

Author : C.A. Jordan
Publisher : Springer Science & Business Media
ISBN 13 : 1489925287
Total Pages : 435 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Electroporation and Electrofusion in Cell Biology by : C.A. Jordan

Download or read book Electroporation and Electrofusion in Cell Biology written by C.A. Jordan and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.

Guide to Electroporation and Electrofusion

Author : Donald C. Chang
Publisher : Academic Press
ISBN 13 : 0080917275
Total Pages : 569 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Guide to Electroporation and Electrofusion by : Donald C. Chang

Download or read book Guide to Electroporation and Electrofusion written by Donald C. Chang and published by Academic Press. This book was released on 2012-12-02 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Key Features * Covers fundamentals of electroporation and electrofusion in detail * Molecular events * Mechanisms * Kinetics * Gives extensive practical information * The latest applications * Controlling parameters to maximize efficiency * Available instrumentation * Presents applications of electroporation and electrofusion in current research situations * State-of-the-art modifications to electrical pulses and generators * Application of electroporation and electrofusion to unique, alternative cell and tissue types * Gives straightforward, detailed, easy-to-follow protocols for * Formation of human hybridomas * Introduction of genetic material into plant cells and pollen * Transfection of mammalian cells * Transformation of bacteria, plants, and yeast * Production of altered embryos * Optimization of electroporation by using reporter genes * Comprehensive and up-to-date * Convenient bench-top format * Approximately 125 illustrations complement the text * Complete references with article titles * Written by leading authorities in electroporation and electrofusion

Electrotransformation of Bacteria

Author : Natalie Eynard
Publisher : Springer Science & Business Media
ISBN 13 : 366204305X
Total Pages : 296 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Electrotransformation of Bacteria by : Natalie Eynard

Download or read book Electrotransformation of Bacteria written by Natalie Eynard and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.

Electroporation in Veterinary Oncology Practice

Author : Joseph A. Impellizeri
Publisher : Springer Nature
ISBN 13 : 3030806685
Total Pages : 354 pages
Book Rating : 4.0/5 (38 download)

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Book Synopsis Electroporation in Veterinary Oncology Practice by : Joseph A. Impellizeri

Download or read book Electroporation in Veterinary Oncology Practice written by Joseph A. Impellizeri and published by Springer Nature. This book was released on 2021-11-20 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first edited collection on veterinary applications of electroporation. Written by an international team of experts, this book presents worldwide emerging therapy options for cancer treatments in veterinary oncology practice. Electroporation offers a precision tool to target cancer cells without destroying surrounding tissue structures. The opening of tumor cell membranes facilitates local control of solid tumors either through the delivery of chemotherapeutics or by direct ablation of tissues using electric fields. In addition, transfer of gene-based products into the cancer cells can be used for genetic vaccination to achieve systemic responses and cancer control. Readers will discover valuable reference texts for practitioner education, including chapters on electrodes for unique anatomical access and treatment planning for deep-seated tumors, different immunotherapy applications with gene electrotransfer, calcium electroporation, irreversible electroporation applications and combinations with other common treatments such as surgery, radiation therapy and chemotherapy. Therapy options with electroporation are gaining interest around the world in both human and veterinary oncology, making this book valuable for oncologists, surgeons, primary care veterinarians, residents, interns and students at veterinary schools, where teaching of Electrochemotherapy will become part of the curriculum. Featuring cross-disciplinary authorship, this work further contributes to advances in comparative medicine and translation research in support of the One Health concept.

Irreversible Electroporation

Author : Boris Rubinsky
Publisher : Springer Science & Business Media
ISBN 13 : 364205420X
Total Pages : 320 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis Irreversible Electroporation by : Boris Rubinsky

Download or read book Irreversible Electroporation written by Boris Rubinsky and published by Springer Science & Business Media. This book was released on 2009-11-25 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-thermal irreversible electroporation is a new minimally invasive surgical p- cedure with unique molecular selectivity attributes – in fact it may be considered the first clinical molecular surgery procedure. Non-thermal irreversible electro- ration is a molecular selective mode of cell ablation that employs brief electrical fields to produce nanoscale defects in the cell membrane, which can lead to cell death, without an effect on any of the other tissue molecules. The electrical fields can be produced through contact by insertion of electrode needles around the undesirable tissue and non-invasively by electromagnetic induction. This new - dition to the medical armamentarium requires the active involvement and is of interest to clinical physicians, medical researchers, mechanical engineers, che- cal engineers, electrical engineers, instrumentation designers, medical companies and many other fields and disciplines that were never exposed in their training to irreversible electroporation or to a similar concept. This edited book is designed to be a comprehensive introduction to the field of irreversible electroporation to those that were not exposed or trained in the field before and can also serve as a reference manual. Irreversible electroporation is broad and interdisciplinary. Therefore, we have made an attempt to cover every one of the various aspects of the field from an introductory basic level to state of the art.

Clinical Aspects of Electroporation

Author : Stephen T. Kee
Publisher : Springer Science & Business Media
ISBN 13 : 1441983635
Total Pages : 251 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Clinical Aspects of Electroporation by : Stephen T. Kee

Download or read book Clinical Aspects of Electroporation written by Stephen T. Kee and published by Springer Science & Business Media. This book was released on 2011-04-06 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electroporation is the forefront in tumor ablation. This book presents the basic principles and clinical applications of electroporation, including the latest research results and patient data. A comprehensive approach to the basic science behind the development of this ground-breaking technique and its introduction into clinical practice, the book discusses the entire spectrum of currently available reversible treatments, the emerging irreversible applications, and their impact on patient care. Clinical Aspects of Electroporation is the first book intended for clinicians on this extremely important and rapidly developing field.

Advanced Electroporation Techniques in Biology and Medicine

Author : Andrei G. Pakhomov
Publisher : CRC Press
ISBN 13 : 1439819076
Total Pages : 542 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Advanced Electroporation Techniques in Biology and Medicine by : Andrei G. Pakhomov

Download or read book Advanced Electroporation Techniques in Biology and Medicine written by Andrei G. Pakhomov and published by CRC Press. This book was released on 2010-06-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: A reflection of the intense study of the effects of electromagnetic fields on living tissues that has taken place during the last decades, Advanced Electroporation Techniques in Biology and Medicine summarizes most recent experimental findings and theories related to permeabilization of biomembranes by pulsed electric fields. Edited by experts and

Electromanipulation of Cells

Author : Ulrich Zimmermann
Publisher : CRC Press
ISBN 13 : 9780849344763
Total Pages : 422 pages
Book Rating : 4.3/5 (447 download)

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Book Synopsis Electromanipulation of Cells by : Ulrich Zimmermann

Download or read book Electromanipulation of Cells written by Ulrich Zimmermann and published by CRC Press. This book was released on 1996-02-16 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electromanipulation of Cells is the first comprehensive, balanced overview of this dynamic discipline. Edited by leading authorities in the field, the book surveys state-of-the-art research as well as recent practical applications of electric field technologies.

Handbook of Biochemistry and Molecular Biology, Fourth Edition

Author : Roger L. Lundblad
Publisher : CRC Press
ISBN 13 : 1466515155
Total Pages : 1098 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Handbook of Biochemistry and Molecular Biology, Fourth Edition by : Roger L. Lundblad

Download or read book Handbook of Biochemistry and Molecular Biology, Fourth Edition written by Roger L. Lundblad and published by CRC Press. This book was released on 2010-05-21 with total page 1098 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fourth edition of the Handbook of Biochemistry and Molecular Biology represents a dramatic revision — the first in two decades — of one of biochemistry's most referenced works. This edition gathers a wealth of information not easily obtained, including information not found on the web. Offering a molecular perspective not available 20 years ago, it provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents. Just a small sampling of the wealth of information found inside the handbook: Buffers and buffer solutions Heat capacities and combustion levels Reagents for the chemical modification of proteins Comprehensive classification system for lipids Biological characteristics of vitamins A huge variety of UV data Recommendations for nomenclature and tables in biochemical thermodynamics Guidelines for NMR measurements for determination of high and low pKa values Viscosity and density tables Chemical and physical properties of various commercial plastics Generic source-based nomenclature for polymers Therapeutic enzymes About the Editors: Roger L. Lundblad, Ph.D. Roger L. Lundblad is a native of San Francisco, California. He received his undergraduate education at Pacific Lutheran University and his PhD degree in biochemistry at the University of Washington. After postdoctoral work in the laboratories of Stanford Moore and William Stein at the Rockefeller University, he joined the faculty of the University of North Carolina at Chapel Hill. He joined the Hyland Division of Baxter Healthcare in 1990. Currently Dr. Lundblad is an independent consultant and writer in biotechnology in Chapel Hill, North Carolina. He is an adjunct Professor of Pathology at the University of North Carolina at Chapel Hill and Editor-in-Chief of the Internet Journal of Genomics and Proteomics. Fiona M. Macdonald, Ph.D., F.R.S.C. Fiona M. Macdonald received her BSc in chemistry from Durham University, UK. She obtained her PhD in inorganic biochemistry at Birkbeck College, University of London, studying under Peter Sadler. Having spent most of her career in scientific publishing, she is now at Taylor and Francis and is involved in developing chemical information products.

Plant Cell Electroporation And Electrofusion Protocols

Author : Jac A. Nickoloff
Publisher : Springer Science & Business Media
ISBN 13 : 1592595421
Total Pages : 209 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Cell Electroporation And Electrofusion Protocols by : Jac A. Nickoloff

Download or read book Plant Cell Electroporation And Electrofusion Protocols written by Jac A. Nickoloff and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna tives to electric field-based methods, citing the advantages and limita tions of the various methods for achieving specific goals.

1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies

Author : Tomaz Jarm
Publisher : Springer
ISBN 13 : 9812878173
Total Pages : 455 pages
Book Rating : 4.8/5 (128 download)

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Book Synopsis 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies by : Tomaz Jarm

Download or read book 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies written by Tomaz Jarm and published by Springer. This book was released on 2015-08-31 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the proceedings of the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies (WC2015). The congress took place in Portorož, Slovenia, during the week of September 6th to 10th, 2015. The scientific part of the Congress covered different aspects of electroporation and related technologies and included the following main topics: · Application of pulsed electric fields technology in food: challenges and opportunities · Electrical impedance measurement for assessment of electroporation yield · Electrochemistry and electroporation · Electroporation meets electrostimulation · Electrotechnologies for food and biomass treatment · Food and biotechnology applications · In vitro electroporation - basic mechanisms · Interfacial behaviour of lipid-assemblies, membranes and cells in electric fields · Irreversible electroporation in clinical use · Medical applications: electrochemotherapy · Medical applications: gene therapy · Non-electric field-based physical methods inducing cell poration and enhanced molecule transfer · Non-thermal plasmas for food safety, environmental applications and medical treatments · PEF for the food industry: fundamentals and applications · PEF proce ss integration - complex process chains and process combinations in the food industry · Predictable animal models · Pulsed electric fields and electroporation technologies in bioeconomy · Veterinary medical applications

Handbook of Biological Effects of Electromagnetic Fields, Third Edition - 2 Volume Set

Author : Charles Polk
Publisher : CRC Press
ISBN 13 : 9780849306419
Total Pages : 636 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Handbook of Biological Effects of Electromagnetic Fields, Third Edition - 2 Volume Set by : Charles Polk

Download or read book Handbook of Biological Effects of Electromagnetic Fields, Third Edition - 2 Volume Set written by Charles Polk and published by CRC Press. This book was released on 1995-12-21 with total page 636 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of this book has been recognized as the standard reference on biological effects of electric and magnetic fields from DC to microwaves. But much has changed in this science since the book's original publication in 1986. With contributions from eighteen leading researchers, this latest edition includes authoritative discussions of many new developments and will quickly become the new, must-have resource handbook. Dielectric properties of biological tissue are thoroughly examined, followed by chapters on physical mechanisms and biological effects of static and extremely low frequency magnetic fields. New chapters on topics that were treated very briefly in the first edition now receive extensive treatment. These topics include electric and magnetic fields for bone and soft tissue repair, electroporation, and epidemiology of ELF health effects. The chapter on computer methods for predicting field intensity has been substantially revised to describe new numerical techniques developed within the last few years and includes calculations of power absorbed in the human head from cellular telephones. The chapter discussing experimental results on RF interaction with living matter now contains information on effects of very high power, very short duration pulses. A new appendix on safety standards is based on the latest publications of governmental, as well as quasi-governmental organizations (such as the U.S. Council on Radiation Protection) in the United States, Europe, and Australia. With all its revisions, this updated version of the CRC Handbook of Biological Effects of Electromagnetic Fields provides the most comprehensive overview available of this rapidly changing science.

Electrotechnologies for Extraction from Food Plants and Biomaterials

Author : Eugene Vorobiev
Publisher : Springer Science & Business Media
ISBN 13 : 0387793747
Total Pages : 286 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Electrotechnologies for Extraction from Food Plants and Biomaterials by : Eugene Vorobiev

Download or read book Electrotechnologies for Extraction from Food Plants and Biomaterials written by Eugene Vorobiev and published by Springer Science & Business Media. This book was released on 2009-02-28 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently, the electrotechnologies based on the effects of pulsed electric fields (PEF), such as ohmic heating (OH) and DC electric field, have gained real interest in the field of food processing. These techniques efficiently enhance methods of extraction from food plants and dehydration of biosolids. The PEF and pulsed OH techniques preserve the nutritional, functional, structural and sensory properties of products better than conventional extraction technologies. The electrofiltration and electro-osmotic dewatering can be very effective for the separation of bioproducts and dehydration of food wastes. The first source book in the field, this book gives an overview the fundamental principles of electrical techniques, electrophysical properties of foods and agricultural products, application of various emerging electrotechnologies for enhancing the solid-liquid separation and drying processes, extraction techniques of pigments, processing methods of different in-plant tissues and biosolids, electro-osmotic dewatering and electrofiltration of biomaterials, recent industrial- scale gains, and other aspects. Each chapter is complementary to other chapters and addresses the latest efforts in the field.

Structure and Dynamics of Membranes

Author : R. Lipowsky
Publisher : Elsevier
ISBN 13 : 9780080541914
Total Pages : 1052 pages
Book Rating : 4.5/5 (419 download)

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Book Synopsis Structure and Dynamics of Membranes by : R. Lipowsky

Download or read book Structure and Dynamics of Membranes written by R. Lipowsky and published by Elsevier. This book was released on 1995-06-15 with total page 1052 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first volume of the Handbook deals with the amazing world of biomembranes and lipid bilayers. Part A describes all aspects related to the morphology of these membranes, beginning with the complex architecture of biomembranes, continues with a description of the bizarre morphology of lipid bilayers and concludes with technological applications of these membranes. The first two chapters deal with biomembranes, providing an introduction to the membranes of eucaryotes and a description of the evolution of membranes. The following chapters are concerned with different aspects of lipids including the physical properties of model membranes composed of lipid-protein mixtures, lateral phase separation of lipids and proteins and measurement of lipid-protein bilayer diffusion. Other chapters deal with the flexibility of fluid bilayers, the closure of bilayers into vesicles which attain a large variety of different shapes, and applications of lipid vesicles and liposomes. Part B covers membrane adhesion, membrane fusion and the interaction of biomembranes with polymer networks such as the cytoskeleton. The first two chapters of this part discuss the generic interactions of membranes from the conceptual point of view. The following two chapters summarize the experimental work on two different bilayer systems. The next chapter deals with the process of contact formation, focal bounding and macroscopic contacts between cells. The cytoskeleton within eucaryotic cells consists of a network of relatively stiff filaments of which three different types of filaments have been identified. As explained in the next chapter much has been recently learned about the interaction of these filaments with the cell membrane. The final two chapters deal with membrane fusion.

Electroporation Protocols

Author : Shulin Li
Publisher : Humana Press
ISBN 13 : 9781617378201
Total Pages : 524 pages
Book Rating : 4.3/5 (782 download)

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Book Synopsis Electroporation Protocols by : Shulin Li

Download or read book Electroporation Protocols written by Shulin Li and published by Humana Press. This book was released on 2010-12-10 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this volume, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA in order to better treat disease. Each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology. Notes on troubleshooting and avoiding known pitfalls are helpfully provided.

Handbook of Food Preservation

Author : M. Shafiur Rahman
Publisher : CRC Press
ISBN 13 : 9780824702090
Total Pages : 832 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Handbook of Food Preservation by : M. Shafiur Rahman

Download or read book Handbook of Food Preservation written by M. Shafiur Rahman and published by CRC Press. This book was released on 1999-01-21 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: With over 2900 references, tables, and drawings, this book covers a wide variety of conventional and potential food preservation techniques. Emphasizing practical, cost-effective, and safe strategies, the book facilitates the selection of the best food ingredients and preservation techniques. It covers postharvest handling, explains conventional preservation methods, details the use of natural antimicrobials, antioxidants, edible coating, nitrites, food packaging, and HACCP in food safety. Highlighting the effects of preservation methods on the functional and sensory properties of foods, the book also features the exact mode or mechanisms involved in each preservation method.