Gold And Silver Staining

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Gold and Silver Staining

Author : Gerhard W. Hacker
Publisher : CRC Press
ISBN 13 : 9780849313929
Total Pages : 264 pages
Book Rating : 4.3/5 (139 download)

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Book Synopsis Gold and Silver Staining by : Gerhard W. Hacker

Download or read book Gold and Silver Staining written by Gerhard W. Hacker and published by CRC Press. This book was released on 2002-04-17 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process. Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps. What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.

Gold and Silver Staining

Author : Gerhard W. Hacker
Publisher : CRC Press
ISBN 13 : 1420040235
Total Pages : 265 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Gold and Silver Staining by : Gerhard W. Hacker

Download or read book Gold and Silver Staining written by Gerhard W. Hacker and published by CRC Press. This book was released on 2002-04-17 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a

Immunogold-Silver Staining

Author : M. A. Hayat
Publisher : CRC Press
ISBN 13 : 1040290531
Total Pages : 337 pages
Book Rating : 4.0/5 (42 download)

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Book Synopsis Immunogold-Silver Staining by : M. A. Hayat

Download or read book Immunogold-Silver Staining written by M. A. Hayat and published by CRC Press. This book was released on 2024-11-01 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

The Glass Painter's Method

Author : David Williams
Publisher :
ISBN 13 : 9781999618933
Total Pages : 242 pages
Book Rating : 4.6/5 (189 download)

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Book Synopsis The Glass Painter's Method by : David Williams

Download or read book The Glass Painter's Method written by David Williams and published by . This book was released on 2018-11-12 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve you well if: You're new to traditional stained glass painting and are uncertain which brushes, paints and tools to buy - this book will tell you what you want to know. The accompanying 12 free videos will also introduce you to the key techniques. You've painted stained glass for a while but still worry "Is it my lack of skill which is the problem, or is it my brushes, paints and tools which stop me achieving the results I want?" - this book will guide you to the answer. And the 23 free designs will give you an excellent resource with which to practise. You're a teacher who wants your students to experience the joy of mastering this wonderful craft - this book will show you the smallest, most effective set of brushes, paints and tools to place before them so they can triumph. From designers and painters Williams & Byrne, and featuring entries from the journal of the forgotten Victorian craftsman Nathaniel Somers, this book will demonstrate how few possessions you really need to paint stained glass. This is Book 1 of The Glass Painter's Method.

Clean My Space

Author : Melissa Maker
Publisher : Penguin
ISBN 13 : 0735214670
Total Pages : 218 pages
Book Rating : 4.7/5 (352 download)

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Book Synopsis Clean My Space by : Melissa Maker

Download or read book Clean My Space written by Melissa Maker and published by Penguin. This book was released on 2017-03-07 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: The wildly popular YouTube star behind Clean My Space presents the breakthrough solution to cleaning better with less effort Melissa Maker is beloved by fans all over the world for her completely re-engineered approach to cleaning. As the dynamic new authority on home and living, Melissa knows that to invest any of our precious time in cleaning, we need to see big, long-lasting results. So, she developed her method to help us get the most out of our effort and keep our homes fresh and welcoming every day. In her long-awaited debut book, she shares her revolutionary 3-step solution: • Identify the most important areas (MIAs) in your home that need attention • Select the proper products, tools, and techniques (PTT) for the job • Implement these new cleaning routines so that they stick Clean My Space takes the chore out of cleaning with Melissa’s incredible tips and cleaning hacks (the power of pretreating!) her lightning fast 5-10 minute “express clean” routines for every room when time is tightest, and her techniques for cleaning even the most daunting places and spaces. And a big bonus: Melissa gives guidance on the best non-toxic, eco-conscious cleaning products and offers natural cleaning solution recipes you can make at home using essential oils to soothe and refresh. With Melissa’s simple groundbreaking method you can truly live in a cleaner, more cheerful, and calming home all the time.

The AGT Cytogenetics Laboratory Manual

Author : Marilyn S. Arsham
Publisher : John Wiley & Sons
ISBN 13 : 1119061229
Total Pages : 1216 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis The AGT Cytogenetics Laboratory Manual by : Marilyn S. Arsham

Download or read book The AGT Cytogenetics Laboratory Manual written by Marilyn S. Arsham and published by John Wiley & Sons. This book was released on 2017-04-24 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

Author : Alexander D. Hyatt
Publisher : CRC Press
ISBN 13 : 9780849367595
Total Pages : 476 pages
Book Rating : 4.3/5 (675 download)

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Book Synopsis Immuno-Gold Electron Microscopy in Virus Diagnosis and Research by : Alexander D. Hyatt

Download or read book Immuno-Gold Electron Microscopy in Virus Diagnosis and Research written by Alexander D. Hyatt and published by CRC Press. This book was released on 1992-11-10 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Nanobiotechnology

Author : Christof M. Niemeyer
Publisher : John Wiley & Sons
ISBN 13 : 3527605916
Total Pages : 491 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Nanobiotechnology by : Christof M. Niemeyer

Download or read book Nanobiotechnology written by Christof M. Niemeyer and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology is the key technology of the 21st century. The possibility to exploit the structures and processes of biomolecules for novel functional materials, biosensors, bioelectronics and medical applications has created the rapidly growing field of nanobiotechnology. Designed as a broad survey of the field, this book combines contributions from bioorganic and bioinorganic chemistry, molecular biology, materials science and bioanalytics to fathom the full scope of current and future developments. It is divided into four main sections: * Interphase Systems * Protein-based Nanostructures * DNA-based Nanostructures * Nanoanalytics Each chapter describes in detail currently available methods and contains numerous references to the primary literature, making this the perfect "field guide" for chemists, biologists and materials scientists who want to explore the fascinating world of nanobiotechnology.

The Albumen & Salted Paper Book

Author : James M. Reilly
Publisher : HP Trade
ISBN 13 :
Total Pages : 152 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis The Albumen & Salted Paper Book by : James M. Reilly

Download or read book The Albumen & Salted Paper Book written by James M. Reilly and published by HP Trade. This book was released on 1980 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Colloidal Gold

Author :
Publisher : Elsevier
ISBN 13 : 0323138810
Total Pages : 565 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Colloidal Gold by :

Download or read book Colloidal Gold written by and published by Elsevier. This book was released on 2012-12-02 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. - Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry - Methods for preparing colloidal gold particles of different sizes - Protein A-gold, protein G-gold, and lectin-gold techniques - The use of resins and thin cryosections - Multiple labeling

Molecular Morphology in Human Tissues

Author : Gerhard W. Hacker
Publisher : CRC Press
ISBN 13 : 0203503481
Total Pages : 328 pages
Book Rating : 4.2/5 (35 download)

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Book Synopsis Molecular Morphology in Human Tissues by : Gerhard W. Hacker

Download or read book Molecular Morphology in Human Tissues written by Gerhard W. Hacker and published by CRC Press. This book was released on 2004-12-29 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more pre

RNA Methodologies

Author : Robert E. Farrell Jr.
Publisher : Elsevier
ISBN 13 : 0080454763
Total Pages : 794 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.

Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Colloidal Gold

Author :
Publisher : Elsevier
ISBN 13 : 0323139027
Total Pages : 517 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Colloidal Gold by :

Download or read book Colloidal Gold written by and published by Elsevier. This book was released on 2012-12-02 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. - Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books - Problems and limitations of techniques are discussed - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields

Analytical Morphology

Author : Jiang Gu
Publisher : Springer Science & Business Media
ISBN 13 : 1461241669
Total Pages : 298 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Analytical Morphology by : Jiang Gu

Download or read book Analytical Morphology written by Jiang Gu and published by Springer Science & Business Media. This book was released on 2013-12-11 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Modern Methods in Analytical Morphology

Author : J. Gu
Publisher : Springer Science & Business Media
ISBN 13 : 1461525322
Total Pages : 441 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Modern Methods in Analytical Morphology by : J. Gu

Download or read book Modern Methods in Analytical Morphology written by J. Gu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: While advances in modem medicine largely parallel our understanding of morphology, discoveries in morphology are propelled by developments of new tools and means to visualize and measure tissue elements. The invention of dissecting, light, fluorescence and electron microscopes together with advances in labeling and staining techniques are among the stepping stones of morphological progress. Today, we are in an exciting new era when classical morphology is being combined with developments from other disciplines. The combination of morphology and immunology resulted in immunocytochemistry; morphology and molecular biology led to in situ hybridization and in situ PCR. Adding computer science to morphology gave birth to image analysis. Combining laser technology and the microsope evolved into confocal microscope. For more than a decade, modem morphology has continued to develop by merging with other disciplines at a rate that is still gathering momentum, providing exciting and dynamic new frontiers for other biological fields. "Modem Methods in Analytical Morphology," based largely on the "First International Workshop on Modem Methods in Analytical Histochemistry, "is an updated review of the current trends in the field. It covers an extensive array of new technical developments in major disciplines of modem morphology. The authors are not only leaders in their fields but also have extensive "hands on" experience with "bench work. " Their chapters are written in a comprehensive manner including discussion of both theoretical considerations and practical applications to give the readers a broad view of the topics covered.

Basic and Advanced Laboratory Techniques in Histopathology and Cytology

Author : Pranab Dey
Publisher : Springer Nature
ISBN 13 : 9811966168
Total Pages : 350 pages
Book Rating : 4.8/5 (119 download)

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Book Synopsis Basic and Advanced Laboratory Techniques in Histopathology and Cytology by : Pranab Dey

Download or read book Basic and Advanced Laboratory Techniques in Histopathology and Cytology written by Pranab Dey and published by Springer Nature. This book was released on 2023-01-01 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

On Divers Arts

Author : Theophilus
Publisher : Courier Corporation
ISBN 13 : 0486145417
Total Pages : 276 pages
Book Rating : 4.4/5 (861 download)

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Book Synopsis On Divers Arts by : Theophilus

Download or read book On Divers Arts written by Theophilus and published by Courier Corporation. This book was released on 2012-04-30 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: "I have made it my concern to hunt out this technique for your study as I learned it by looking and listening." On Divers Arts, c. 1122, is the oldest extant manual on artistic crafts to be written by a practicing artist. Before Theophilus, manuscripts on the arts came from scholars and philosophers standing outside the actual profession. On Divers Arts describes actual 12th-century techniques in painting, glass, and metalwork, which the Benedictine author wished to pass on to those gifted by God with a talent for making beautiful things. Theophilus teaches, with rigorous attention to fact but also with great reverence the making of pigments for fresco painting, the manufacture of glue, the technique of gold leaf on parchment (the first recorded European reference to true paper), how to blow glass and design stained glass windows, how to fashion gold and silver chalices, and how to make a pipe organ and church bells. Precise instruction on enameling, chasing, repoussé, niello, and beaded wire work prove Theophilus's first-hand knowledge of his craft. While 90 percent of Theophilus's writing is sound technical knowledge, medieval folk lore occasionally spices his text: "Tools are also made harder by hardening them in the urine of a small red-headed boy than by doing so in plain water." But the magnificent fact of On Divers Art remains its status as the first technical treatise on painting, glass, and metalwork, for which actual specimens still survive. The editors have taken care to ensure both philological and technological accuracy for this authoritative edition of a medieval classic, a manual of great importance to craftsmen, historians of art and science, and all who delight in the making of the beautiful.